Oceanococcus atlanticus
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3284 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y1SCE4|A0A1Y1SCE4_9GAMM LuxR family transcriptional regulator OS=Oceanococcus atlanticus OX=1317117 GN=ATO7_11898 PE=4 SV=1
MM1 pKa = 7.73 TIQKK5 pKa = 8.93 TGLAVLGLTAAAMVGCGGGGGGGGSSDD32 pKa = 3.87 PTPTPTPTPTATPGAGDD49 pKa = 4.08 SVCDD53 pKa = 3.79 EE54 pKa = 4.75 SFVSCSGTNATLSGTIDD71 pKa = 3.25 KK72 pKa = 10.89 DD73 pKa = 3.72 FTLSTDD79 pKa = 4.16 FNWFLDD85 pKa = 3.59 GFVQVGNGNVEE96 pKa = 3.97 ITSNAQANDD105 pKa = 3.05 LRR107 pKa = 11.84 NNGVEE112 pKa = 4.01 LTIPAGTHH120 pKa = 4.03 VKK122 pKa = 10.46 ALEE125 pKa = 4.13 TGVLIVTRR133 pKa = 11.84 GNRR136 pKa = 11.84 LIANGTAANPITFSSVDD153 pKa = 3.66 DD154 pKa = 4.67 GFDD157 pKa = 4.19 GLGEE161 pKa = 3.94 WGGVVLQGFAPQFGAGGTGNCFGDD185 pKa = 3.54 NNYY188 pKa = 10.34 CNVAGEE194 pKa = 4.55 GGSDD198 pKa = 3.14 VAFYY202 pKa = 10.6 GGKK205 pKa = 10.14 YY206 pKa = 9.24 PADD209 pKa = 3.35 NSGSIRR215 pKa = 11.84 YY216 pKa = 8.84 VRR218 pKa = 11.84 IAEE221 pKa = 4.45 GGLVAGPNNEE231 pKa = 4.11 VNGLTLQGVGHH242 pKa = 7.2 ATTIEE247 pKa = 4.08 YY248 pKa = 10.21 VQVHH252 pKa = 6.33 NNLDD256 pKa = 4.23 DD257 pKa = 4.11 GVEE260 pKa = 4.1 WFGGTVNAKK269 pKa = 10.18 YY270 pKa = 10.37 LVLTGNDD277 pKa = 4.58 DD278 pKa = 4.52 DD279 pKa = 7.02 DD280 pKa = 4.77 IDD282 pKa = 4.24 YY283 pKa = 11.56 DD284 pKa = 3.74 EE285 pKa = 5.51 GYY287 pKa = 10.28 KK288 pKa = 11.09 GNIQFAIVKK297 pKa = 9.74 KK298 pKa = 10.32 SSNDD302 pKa = 3.15 APQGSNDD309 pKa = 3.25 PRR311 pKa = 11.84 GIEE314 pKa = 4.23 ANSSDD319 pKa = 4.26 EE320 pKa = 4.74 DD321 pKa = 3.88 FVPQTTASLANILLIGNTVSANEE344 pKa = 3.65 PGMRR348 pKa = 11.84 LRR350 pKa = 11.84 GALTTSIFNTALVGWDD366 pKa = 3.71 KK367 pKa = 11.61 GCIRR371 pKa = 11.84 IDD373 pKa = 4.42 DD374 pKa = 4.66 ADD376 pKa = 3.99 TNADD380 pKa = 3.57 DD381 pKa = 5.35 VADD384 pKa = 3.67 QFSNVSLEE392 pKa = 4.2 NVFGDD397 pKa = 4.21 CAGGFYY403 pKa = 8.92 THH405 pKa = 7.11 EE406 pKa = 5.06 DD407 pKa = 3.52 ADD409 pKa = 4.05 STAGGVGSQTISLDD423 pKa = 3.07 DD424 pKa = 4.2 AYY426 pKa = 10.73 AINEE430 pKa = 4.19 GFASLASATNIVAVDD445 pKa = 3.69 NGSGFAFEE453 pKa = 4.22 TTDD456 pKa = 3.21 YY457 pKa = 11.1 VGAVEE462 pKa = 5.33 PGTSMGDD469 pKa = 3.13 AWWAGWTIPGSLSDD483 pKa = 4.44 ADD485 pKa = 4.12 EE486 pKa = 4.95 APASASFVSCNDD498 pKa = 2.51 STMICEE504 pKa = 4.08 VTGTIDD510 pKa = 3.22 EE511 pKa = 5.47 DD512 pKa = 4.25 YY513 pKa = 10.38 TFVAGYY519 pKa = 7.95 TWVLNGLVKK528 pKa = 10.94 VGDD531 pKa = 3.99 GNVEE535 pKa = 4.06 INSEE539 pKa = 4.12 SQADD543 pKa = 3.76 QIKK546 pKa = 10.8 ADD548 pKa = 3.79 GVTLTIRR555 pKa = 11.84 PGVDD559 pKa = 2.95 VKK561 pKa = 10.78 GTEE564 pKa = 3.98 TGVLVVTRR572 pKa = 11.84 GSKK575 pKa = 10.73 LIANGSASAPITFSSLDD592 pKa = 3.57 EE593 pKa = 4.55 NFDD596 pKa = 4.45 GLGEE600 pKa = 4.03 WGGVVLQGFAPQFGAGNTGNCHH622 pKa = 6.16 GANSYY627 pKa = 11.29 CNVAGEE633 pKa = 4.52 GGGGVAFYY641 pKa = 10.96 GGDD644 pKa = 3.65 DD645 pKa = 3.66 PADD648 pKa = 3.39 NSGSLRR654 pKa = 11.84 YY655 pKa = 10.08 VRR657 pKa = 11.84 IAEE660 pKa = 4.48 GGLVAGPNNEE670 pKa = 4.11 VNGLTLQGVGYY681 pKa = 7.25 MTSLDD686 pKa = 3.98 YY687 pKa = 11.1 IQVHH691 pKa = 6.17 NNLDD695 pKa = 4.34 DD696 pKa = 4.11 GVEE699 pKa = 4.1 WFGGTVNAKK708 pKa = 10.11 HH709 pKa = 6.32 LVLTGNDD716 pKa = 3.82 DD717 pKa = 4.09 DD718 pKa = 7.3 DD719 pKa = 5.42 IDD721 pKa = 4.24 FDD723 pKa = 4.14 EE724 pKa = 5.78 GYY726 pKa = 10.36 KK727 pKa = 11.14 GNIQYY732 pKa = 10.93 AIVIKK737 pKa = 10.5 SDD739 pKa = 2.86 NATPQGSNDD748 pKa = 3.19 PRR750 pKa = 11.84 GIEE753 pKa = 4.23 ANSSDD758 pKa = 4.19 EE759 pKa = 5.17 DD760 pKa = 4.07 YY761 pKa = 11.56 VPQTQATLANILLVGNTVSANEE783 pKa = 3.65 PGMRR787 pKa = 11.84 LRR789 pKa = 11.84 GALTVSIFNTALTGWDD805 pKa = 3.74 EE806 pKa = 4.12 GCIRR810 pKa = 11.84 IDD812 pKa = 4.59 DD813 pKa = 4.52 ADD815 pKa = 4.12 TNGDD819 pKa = 3.62 DD820 pKa = 4.06 TNDD823 pKa = 3.39 AFSDD827 pKa = 3.75 VTLVNILGNCSGGLYY842 pKa = 6.85 THH844 pKa = 7.52 RR845 pKa = 11.84 DD846 pKa = 3.1 ADD848 pKa = 3.67 SQTNYY853 pKa = 10.05 VSNAVNLNQALVVTGAAAQVSAPSITAVANGSGFSFDD890 pKa = 3.15 EE891 pKa = 4.15 TNYY894 pKa = 10.1 IGAVAPGTNVADD906 pKa = 3.78 AWWNGWTIPNSVSVDD921 pKa = 3.17
Molecular weight: 94.81 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.675
IPC2_protein 3.706
IPC_protein 3.757
Toseland 3.528
ProMoST 3.91
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.706
Rodwell 3.579
Grimsley 3.427
Solomon 3.757
Lehninger 3.706
Nozaki 3.859
DTASelect 4.139
Thurlkill 3.579
EMBOSS 3.706
Sillero 3.884
Patrickios 1.303
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.786
Protein with the highest isoelectric point:
>tr|A0A1Y1SIR0|A0A1Y1SIR0_9GAMM Uncharacterized protein OS=Oceanococcus atlanticus OX=1317117 GN=ATO7_04910 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVIKK11 pKa = 10.52 RR12 pKa = 11.84 KK13 pKa = 8.26 RR14 pKa = 11.84 THH16 pKa = 5.92 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.13 GGRR28 pKa = 11.84 RR29 pKa = 11.84 VINARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.85 GRR39 pKa = 11.84 HH40 pKa = 5.12 SLTVV44 pKa = 3.06
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3284
0
3284
1129603
27
2975
344.0
37.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
11.645 ± 0.06
1.003 ± 0.015
6.12 ± 0.036
5.63 ± 0.036
3.495 ± 0.022
7.908 ± 0.041
2.378 ± 0.021
4.671 ± 0.032
2.822 ± 0.034
10.96 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.364 ± 0.022
2.958 ± 0.026
4.908 ± 0.026
4.576 ± 0.029
6.897 ± 0.043
5.877 ± 0.035
4.729 ± 0.031
7.059 ± 0.038
1.492 ± 0.017
2.507 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here