Streptomyces gilvigriseus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptomycetales; Streptomycetaceae; Streptomyces

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4337 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J7C8L0|A0A1J7C8L0_9ACTN Helix-turn-helix transcriptional regulator OS=Streptomyces gilvigriseus OX=1428644 GN=BIV57_08895 PE=4 SV=1
MM1 pKa = 7.52AAGAEE6 pKa = 4.42PTDD9 pKa = 3.61QTGPADD15 pKa = 4.14PSDD18 pKa = 3.81PADD21 pKa = 4.15PSDD24 pKa = 3.9AEE26 pKa = 3.96PRR28 pKa = 11.84AACVLCGKK36 pKa = 8.37PTDD39 pKa = 4.98LPDD42 pKa = 3.65STPGRR47 pKa = 11.84TLCPRR52 pKa = 11.84CDD54 pKa = 3.28WEE56 pKa = 4.28EE57 pKa = 4.17TVNSQCTGG65 pKa = 3.06

Molecular weight: 6.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J7C7F2|A0A1J7C7F2_9ACTN Uncharacterized protein OS=Streptomyces gilvigriseus OX=1428644 GN=BIV57_10855 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84ARR15 pKa = 11.84THH17 pKa = 5.69GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84GILASRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.63GRR40 pKa = 11.84AALSAA45 pKa = 3.96

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4337

0

4337

1434332

29

2330

330.7

35.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.657 ± 0.065

0.747 ± 0.011

5.785 ± 0.032

5.479 ± 0.044

2.611 ± 0.021

9.741 ± 0.041

2.242 ± 0.017

3.085 ± 0.025

2.109 ± 0.032

10.349 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.751 ± 0.015

1.801 ± 0.021

6.233 ± 0.04

2.791 ± 0.028

8.193 ± 0.047

4.968 ± 0.032

5.772 ± 0.043

8.189 ± 0.032

1.506 ± 0.015

1.989 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski