Bat polyomavirus 6a
Average proteome isoelectric point is 5.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0D5ZYL6|A0A0D5ZYL6_9POLY Small t antigen OS=Bat polyomavirus 6a OX=1623685 PE=4 SV=1
MM1 pKa = 7.31 GALLAVLAEE10 pKa = 4.31 VFDD13 pKa = 4.55 LAATTGLSVDD23 pKa = 3.57 SFLTGEE29 pKa = 4.4 AFTTAEE35 pKa = 4.17 VLQSHH40 pKa = 7.13 IANLVTYY47 pKa = 9.75 GGLSEE52 pKa = 5.27 AEE54 pKa = 3.8 ALAAAEE60 pKa = 4.48 VSQEE64 pKa = 4.02 AYY66 pKa = 10.37 SALTSLTSNFPRR78 pKa = 11.84 AFAALAGTEE87 pKa = 3.82 ALAVGSLVIGSATAAALAPYY107 pKa = 8.79 TFDD110 pKa = 3.22 YY111 pKa = 8.75 STPIANLNVDD121 pKa = 3.4 MALQVWQPNWDD132 pKa = 4.25 DD133 pKa = 3.23 IFFPGVVPFARR144 pKa = 11.84 IVNYY148 pKa = 9.84 IDD150 pKa = 3.56 PANWAAYY157 pKa = 9.76 LYY159 pKa = 9.93 QAVGRR164 pKa = 11.84 YY165 pKa = 7.22 FWEE168 pKa = 4.19 TAQRR172 pKa = 11.84 TGQHH176 pKa = 6.24 LIEE179 pKa = 5.69 HH180 pKa = 6.53 EE181 pKa = 4.15 VRR183 pKa = 11.84 EE184 pKa = 4.32 VARR187 pKa = 11.84 EE188 pKa = 3.76 VGQRR192 pKa = 11.84 TVQSVSEE199 pKa = 3.87 TLARR203 pKa = 11.84 YY204 pKa = 9.22 FEE206 pKa = 4.06 NARR209 pKa = 11.84 WAVSHH214 pKa = 6.78 LSSNVYY220 pKa = 10.4 SGLQNYY226 pKa = 7.06 YY227 pKa = 10.03 TEE229 pKa = 5.13 LGPLRR234 pKa = 11.84 PHH236 pKa = 5.8 QVRR239 pKa = 11.84 AVNRR243 pKa = 11.84 RR244 pKa = 11.84 LGRR247 pKa = 11.84 EE248 pKa = 3.47 LPTRR252 pKa = 11.84 YY253 pKa = 9.72 NLEE256 pKa = 3.98 TPRR259 pKa = 11.84 PQDD262 pKa = 3.09 KK263 pKa = 10.96 ASAQYY268 pKa = 8.41 VTKK271 pKa = 10.7 SDD273 pKa = 3.74 APGGAHH279 pKa = 6.4 QRR281 pKa = 11.84 VTPDD285 pKa = 2.33 WMLPLILGLYY295 pKa = 10.55 GDD297 pKa = 4.68 ITPSWEE303 pKa = 3.81 ATLEE307 pKa = 4.05 DD308 pKa = 3.98 LEE310 pKa = 4.45 EE311 pKa = 4.39 EE312 pKa = 4.35 EE313 pKa = 4.89 EE314 pKa = 4.29 EE315 pKa = 4.78 EE316 pKa = 4.66 EE317 pKa = 4.79 NGPKK321 pKa = 9.81 KK322 pKa = 10.36 KK323 pKa = 10.22 KK324 pKa = 9.31 PRR326 pKa = 11.84 RR327 pKa = 11.84 GKK329 pKa = 10.15 KK330 pKa = 5.74 QTKK333 pKa = 9.91 ANSNSSAA340 pKa = 3.2
Molecular weight: 37.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.976
IPC2_protein 5.041
IPC_protein 4.965
Toseland 4.889
ProMoST 5.067
Dawson 4.927
Bjellqvist 5.054
Wikipedia 4.762
Rodwell 4.851
Grimsley 4.813
Solomon 4.914
Lehninger 4.876
Nozaki 5.041
DTASelect 5.13
Thurlkill 4.876
EMBOSS 4.8
Sillero 5.118
Patrickios 3.91
IPC_peptide 4.927
IPC2_peptide 5.118
IPC2.peptide.svr19 5.064
Protein with the highest isoelectric point:
>tr|A0A0D5ZZ24|A0A0D5ZZ24_9POLY Large T antigen OS=Bat polyomavirus 6a OX=1623685 PE=4 SV=1
MM1 pKa = 7.66 DD2 pKa = 4.87 HH3 pKa = 7.31 ALTRR7 pKa = 11.84 EE8 pKa = 3.84 EE9 pKa = 4.5 SVRR12 pKa = 11.84 LMQLLKK18 pKa = 11.02 LPMEE22 pKa = 4.28 QYY24 pKa = 11.86 GNFSLMRR31 pKa = 11.84 KK32 pKa = 9.53 AFLRR36 pKa = 11.84 EE37 pKa = 4.23 CKK39 pKa = 10.0 NLHH42 pKa = 6.42 PDD44 pKa = 2.95 KK45 pKa = 11.24 GGNPEE50 pKa = 4.08 LAKK53 pKa = 10.59 EE54 pKa = 4.41 LITLYY59 pKa = 10.67 RR60 pKa = 11.84 KK61 pKa = 10.23 LEE63 pKa = 4.16 EE64 pKa = 3.86 QAPPYY69 pKa = 7.55 GTPEE73 pKa = 3.51 WDD75 pKa = 3.28 RR76 pKa = 11.84 WWEE79 pKa = 3.84 EE80 pKa = 3.8 FNDD83 pKa = 4.17 LFCHH87 pKa = 5.08 EE88 pKa = 4.52 TFDD91 pKa = 4.9 RR92 pKa = 11.84 SDD94 pKa = 3.67 DD95 pKa = 3.71 EE96 pKa = 4.29 EE97 pKa = 4.28 EE98 pKa = 4.03 QRR100 pKa = 11.84 KK101 pKa = 8.95 RR102 pKa = 11.84 PASPEE107 pKa = 4.01 SEE109 pKa = 4.47 PEE111 pKa = 4.14 CSQATPPKK119 pKa = 9.81 KK120 pKa = 9.94 KK121 pKa = 10.54 KK122 pKa = 9.62 KK123 pKa = 9.8 DD124 pKa = 3.51 HH125 pKa = 6.69 PKK127 pKa = 11.04 DD128 pKa = 3.55 MPEE131 pKa = 4.59 DD132 pKa = 3.57 LQQFLSNAILSNKK145 pKa = 7.54 TVSSFLIFTTAEE157 pKa = 4.16 KK158 pKa = 9.98 STLLYY163 pKa = 10.79 GKK165 pKa = 10.29 LRR167 pKa = 11.84 DD168 pKa = 3.55 RR169 pKa = 11.84 FKK171 pKa = 10.01 ATFVSRR177 pKa = 11.84 HH178 pKa = 4.94 KK179 pKa = 10.58 QQGSDD184 pKa = 3.11 GEE186 pKa = 4.39 AFVFLITPSRR196 pKa = 11.84 HH197 pKa = 4.53 RR198 pKa = 11.84 VSAINNFCNSLCSVSFIIVKK218 pKa = 9.98 GVIKK222 pKa = 10.19 EE223 pKa = 4.03 YY224 pKa = 10.8 NLYY227 pKa = 10.39 VHH229 pKa = 6.95 LCVEE233 pKa = 5.04 PYY235 pKa = 10.91 VKK237 pKa = 10.38 LQEE240 pKa = 4.7 SIPGGLSRR248 pKa = 11.84 DD249 pKa = 3.68 FFDD252 pKa = 4.47 TPEE255 pKa = 3.82 EE256 pKa = 3.88 AAKK259 pKa = 10.37 NVSWKK264 pKa = 9.92 MIAEE268 pKa = 4.53 FALDD272 pKa = 4.64 LMCDD276 pKa = 4.07 DD277 pKa = 4.91 IFLLMGMYY285 pKa = 10.12 KK286 pKa = 10.2 EE287 pKa = 4.19 FAEE290 pKa = 5.05 DD291 pKa = 3.41 PKK293 pKa = 11.48 SCAKK297 pKa = 10.55 CEE299 pKa = 3.77 AKK301 pKa = 10.27 IISDD305 pKa = 3.76 HH306 pKa = 5.72 FQNHH310 pKa = 4.18 FHH312 pKa = 6.88 HH313 pKa = 6.85 YY314 pKa = 9.89 EE315 pKa = 3.87 NALLFLDD322 pKa = 4.82 CRR324 pKa = 11.84 NQKK327 pKa = 10.21 AICQQAVDD335 pKa = 3.89 GVIAHH340 pKa = 6.58 RR341 pKa = 11.84 RR342 pKa = 11.84 VQTAQLSRR350 pKa = 11.84 EE351 pKa = 3.93 QQLAEE356 pKa = 3.92 RR357 pKa = 11.84 FKK359 pKa = 11.32 KK360 pKa = 10.61 LFEE363 pKa = 4.26 KK364 pKa = 10.39 MEE366 pKa = 4.3 NLFGARR372 pKa = 11.84 SKK374 pKa = 9.88 VTLRR378 pKa = 11.84 TYY380 pKa = 9.45 MAGVAWFDD388 pKa = 3.69 SLFPDD393 pKa = 4.28 KK394 pKa = 11.0 SFKK397 pKa = 10.6 EE398 pKa = 3.84 LLLDD402 pKa = 4.37 FLEE405 pKa = 4.74 CMVNNVPKK413 pKa = 10.08 KK414 pKa = 10.23 RR415 pKa = 11.84 YY416 pKa = 8.33 WFFTGPVNTGKK427 pKa = 7.57 TTLAAALLDD436 pKa = 3.8 LCGGKK441 pKa = 9.46 SLNVNMPFEE450 pKa = 4.38 KK451 pKa = 10.86 LNFEE455 pKa = 4.68 LGVAIDD461 pKa = 3.38 QFMVIFEE468 pKa = 4.27 DD469 pKa = 3.55 VKK471 pKa = 11.36 GQVSEE476 pKa = 4.31 NKK478 pKa = 9.82 NLPTGQGINNLDD490 pKa = 3.79 HH491 pKa = 7.04 LRR493 pKa = 11.84 DD494 pKa = 3.89 YY495 pKa = 11.54 LDD497 pKa = 3.82 GAVKK501 pKa = 10.66 VNLEE505 pKa = 4.2 KK506 pKa = 10.85 KK507 pKa = 9.75 HH508 pKa = 6.21 LNKK511 pKa = 10.01 KK512 pKa = 8.41 SQIFPPGIVTANEE525 pKa = 3.9 YY526 pKa = 10.32 KK527 pKa = 10.82 VPFTLRR533 pKa = 11.84 VRR535 pKa = 11.84 FCKK538 pKa = 9.96 TLTFQFRR545 pKa = 11.84 KK546 pKa = 10.01 NLYY549 pKa = 8.98 LSLRR553 pKa = 11.84 KK554 pKa = 8.42 TEE556 pKa = 4.53 DD557 pKa = 2.94 LGTYY561 pKa = 9.68 RR562 pKa = 11.84 VLQNGICLLLFLVYY576 pKa = 10.18 HH577 pKa = 6.27 CEE579 pKa = 3.76 IEE581 pKa = 4.75 DD582 pKa = 4.44 FSEE585 pKa = 4.43 CLHH588 pKa = 6.1 KK589 pKa = 9.58 TVEE592 pKa = 3.95 QWKK595 pKa = 9.43 EE596 pKa = 3.82 RR597 pKa = 11.84 ISQEE601 pKa = 3.86 VSLGDD606 pKa = 3.63 YY607 pKa = 11.19 LDD609 pKa = 4.2 FKK611 pKa = 11.84 SNIQKK616 pKa = 10.66 GNYY619 pKa = 8.55 ILQTTEE625 pKa = 3.66 SQGPSTQEE633 pKa = 3.91 CEE635 pKa = 4.25 TEE637 pKa = 4.27 DD638 pKa = 3.99 FVNN641 pKa = 3.91
Molecular weight: 74.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.998
IPC2_protein 6.02
IPC_protein 6.071
Toseland 6.3
ProMoST 6.453
Dawson 6.389
Bjellqvist 6.389
Wikipedia 6.389
Rodwell 6.376
Grimsley 6.402
Solomon 6.389
Lehninger 6.376
Nozaki 6.664
DTASelect 6.825
Thurlkill 6.854
EMBOSS 6.81
Sillero 6.766
Patrickios 4.279
IPC_peptide 6.402
IPC2_peptide 6.634
IPC2.peptide.svr19 6.665
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4
0
4
1509
162
641
377.3
42.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.025 ± 1.657
2.319 ± 0.775
4.771 ± 0.403
7.753 ± 0.615
5.103 ± 0.938
5.5 ± 0.753
1.922 ± 0.382
3.777 ± 0.396
6.296 ± 1.191
10.404 ± 0.649
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.988 ± 0.285
5.036 ± 0.335
5.434 ± 0.898
4.705 ± 0.127
4.904 ± 0.318
5.898 ± 0.268
5.699 ± 0.653
6.494 ± 0.665
1.458 ± 0.429
3.512 ± 0.385
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here