Cytobacillus horneckiae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Cytobacillus

Average proteome isoelectric point is 6.3

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5301 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N0ZCJ1|A0A2N0ZCJ1_9BACI Prolyl 4-hydroxylase subunit alpha OS=Cytobacillus horneckiae OX=549687 GN=CWS20_20390 PE=4 SV=1
MM1 pKa = 7.74DD2 pKa = 3.24QRR4 pKa = 11.84LIYY7 pKa = 10.9KK8 pKa = 8.06MVQNCLKK15 pKa = 10.49QYY17 pKa = 10.16MEE19 pKa = 5.37DD20 pKa = 3.79PSSLKK25 pKa = 9.63MDD27 pKa = 3.64SDD29 pKa = 4.11AFAEE33 pKa = 4.24IYY35 pKa = 10.54EE36 pKa = 4.54KK37 pKa = 10.62IKK39 pKa = 10.09TSQNEE44 pKa = 4.15DD45 pKa = 3.31LDD47 pKa = 4.57SDD49 pKa = 4.16LNEE52 pKa = 4.88IIDD55 pKa = 4.11DD56 pKa = 3.85VVYY59 pKa = 10.24GYY61 pKa = 8.84VTDD64 pKa = 3.57SPYY67 pKa = 10.85FF68 pKa = 3.74

Molecular weight: 8.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N0Z8R8|A0A2N0Z8R8_9BACI Uncharacterized protein OS=Cytobacillus horneckiae OX=549687 GN=CWS20_26790 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.59RR3 pKa = 11.84TFQPNKK9 pKa = 8.24RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84SKK14 pKa = 9.59VHH16 pKa = 5.83GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSATGRR28 pKa = 11.84NVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.2 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5301

0

5301

1490556

26

2807

281.2

31.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.996 ± 0.036

0.734 ± 0.011

5.042 ± 0.031

7.621 ± 0.038

4.574 ± 0.031

6.74 ± 0.029

2.073 ± 0.017

8.334 ± 0.04

7.162 ± 0.035

9.711 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.824 ± 0.016

4.729 ± 0.029

3.476 ± 0.021

3.661 ± 0.026

3.821 ± 0.022

6.197 ± 0.028

5.172 ± 0.022

6.582 ± 0.026

0.994 ± 0.01

3.556 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski