Sedimentibacter saalensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Tissierellia; Tissierellia incertae sedis; Sedimentibacter

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3564 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A562JH01|A0A562JH01_9FIRM Vacuolar-type H+-ATPase subunit E/Vma4 OS=Sedimentibacter saalensis OX=130788 GN=LY60_00812 PE=3 SV=1
MM1 pKa = 7.42KK2 pKa = 10.37VLSEE6 pKa = 4.12LKK8 pKa = 10.29YY9 pKa = 10.31SEE11 pKa = 3.71SHH13 pKa = 5.34EE14 pKa = 4.26WVKK17 pKa = 11.14VEE19 pKa = 4.55GNKK22 pKa = 10.41AYY24 pKa = 9.76IGITDD29 pKa = 3.67YY30 pKa = 11.65AQDD33 pKa = 3.45HH34 pKa = 6.84LGDD37 pKa = 3.78VVYY40 pKa = 11.29VDD42 pKa = 4.4LPEE45 pKa = 5.0VDD47 pKa = 3.96TEE49 pKa = 4.21IEE51 pKa = 4.07AGGQCIVLEE60 pKa = 4.31SVKK63 pKa = 10.54AASEE67 pKa = 4.34VFSPLSGMVVEE78 pKa = 4.93INEE81 pKa = 4.39SLSDD85 pKa = 3.54NPSFINEE92 pKa = 4.24SPYY95 pKa = 10.35EE96 pKa = 3.76AWLIAVEE103 pKa = 4.15MSDD106 pKa = 3.42PSEE109 pKa = 4.11LSGLMSAEE117 pKa = 4.58DD118 pKa = 3.76YY119 pKa = 11.39EE120 pKa = 4.86KK121 pKa = 10.65ICDD124 pKa = 3.47

Molecular weight:
13.67 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A562JL39|A0A562JL39_9FIRM Dienelactone hydrolase OS=Sedimentibacter saalensis OX=130788 GN=LY60_00030 PE=4 SV=1
MM1 pKa = 7.95SSRR4 pKa = 11.84QGGNNSRR11 pKa = 11.84EE12 pKa = 4.08DD13 pKa = 3.29RR14 pKa = 11.84GDD16 pKa = 3.33RR17 pKa = 11.84NDD19 pKa = 3.12RR20 pKa = 11.84MGNKK24 pKa = 9.07RR25 pKa = 11.84RR26 pKa = 11.84PRR28 pKa = 11.84KK29 pKa = 9.39KK30 pKa = 9.55VCQFCQEE37 pKa = 4.15KK38 pKa = 9.04ATHH41 pKa = 6.84IDD43 pKa = 3.66YY44 pKa = 11.15KK45 pKa = 11.37DD46 pKa = 3.18LGKK49 pKa = 10.5LKK51 pKa = 10.51KK52 pKa = 9.46YY53 pKa = 4.42TTEE56 pKa = 3.8RR57 pKa = 11.84GKK59 pKa = 10.14ILPRR63 pKa = 11.84RR64 pKa = 11.84ITGACAKK71 pKa = 8.41HH72 pKa = 4.94QRR74 pKa = 11.84MVTRR78 pKa = 11.84AIKK81 pKa = 10.06RR82 pKa = 11.84ARR84 pKa = 11.84TIALMPYY91 pKa = 9.89VADD94 pKa = 3.71

Molecular weight:
10.93 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3564

0

3564

1119672

38

2069

314.2

35.24

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.467 ± 0.047

1.171 ± 0.018

5.594 ± 0.034

7.432 ± 0.045

4.331 ± 0.031

6.706 ± 0.04

1.485 ± 0.017

9.148 ± 0.042

7.895 ± 0.037

8.726 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.064 ± 0.024

5.82 ± 0.028

3.027 ± 0.022

2.498 ± 0.018

3.363 ± 0.029

6.408 ± 0.029

5.21 ± 0.032

6.871 ± 0.032

0.7 ± 0.011

4.082 ± 0.032

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski