Sedimentibacter saalensis
Average proteome isoelectric point is 6.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3564 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A562JH01|A0A562JH01_9FIRM Vacuolar-type H+-ATPase subunit E/Vma4 OS=Sedimentibacter saalensis OX=130788 GN=LY60_00812 PE=3 SV=1
MM1 pKa = 7.42 KK2 pKa = 10.37 VLSEE6 pKa = 4.12 LKK8 pKa = 10.29 YY9 pKa = 10.31 SEE11 pKa = 3.71 SHH13 pKa = 5.34 EE14 pKa = 4.26 WVKK17 pKa = 11.14 VEE19 pKa = 4.55 GNKK22 pKa = 10.41 AYY24 pKa = 9.76 IGITDD29 pKa = 3.67 YY30 pKa = 11.65 AQDD33 pKa = 3.45 HH34 pKa = 6.84 LGDD37 pKa = 3.78 VVYY40 pKa = 11.29 VDD42 pKa = 4.4 LPEE45 pKa = 5.0 VDD47 pKa = 3.96 TEE49 pKa = 4.21 IEE51 pKa = 4.07 AGGQCIVLEE60 pKa = 4.31 SVKK63 pKa = 10.54 AASEE67 pKa = 4.34 VFSPLSGMVVEE78 pKa = 4.93 INEE81 pKa = 4.39 SLSDD85 pKa = 3.54 NPSFINEE92 pKa = 4.24 SPYY95 pKa = 10.35 EE96 pKa = 3.76 AWLIAVEE103 pKa = 4.15 MSDD106 pKa = 3.42 PSEE109 pKa = 4.11 LSGLMSAEE117 pKa = 4.58 DD118 pKa = 3.76 YY119 pKa = 11.39 EE120 pKa = 4.86 KK121 pKa = 10.65 ICDD124 pKa = 3.47
Molecular weight: 13.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.762
IPC2_protein 3.923
IPC_protein 3.846
Toseland 3.681
ProMoST 3.923
Dawson 3.808
Bjellqvist 3.999
Wikipedia 3.694
Rodwell 3.694
Grimsley 3.592
Solomon 3.795
Lehninger 3.745
Nozaki 3.923
DTASelect 4.05
Thurlkill 3.706
EMBOSS 3.706
Sillero 3.961
Patrickios 0.947
IPC_peptide 3.795
IPC2_peptide 3.948
IPC2.peptide.svr19 3.883
Protein with the highest isoelectric point:
>tr|A0A562JL39|A0A562JL39_9FIRM Dienelactone hydrolase OS=Sedimentibacter saalensis OX=130788 GN=LY60_00030 PE=4 SV=1
MM1 pKa = 7.95 SSRR4 pKa = 11.84 QGGNNSRR11 pKa = 11.84 EE12 pKa = 4.08 DD13 pKa = 3.29 RR14 pKa = 11.84 GDD16 pKa = 3.33 RR17 pKa = 11.84 NDD19 pKa = 3.12 RR20 pKa = 11.84 MGNKK24 pKa = 9.07 RR25 pKa = 11.84 RR26 pKa = 11.84 PRR28 pKa = 11.84 KK29 pKa = 9.39 KK30 pKa = 9.55 VCQFCQEE37 pKa = 4.15 KK38 pKa = 9.04 ATHH41 pKa = 6.84 IDD43 pKa = 3.66 YY44 pKa = 11.15 KK45 pKa = 11.37 DD46 pKa = 3.18 LGKK49 pKa = 10.5 LKK51 pKa = 10.51 KK52 pKa = 9.46 YY53 pKa = 4.42 TTEE56 pKa = 3.8 RR57 pKa = 11.84 GKK59 pKa = 10.14 ILPRR63 pKa = 11.84 RR64 pKa = 11.84 ITGACAKK71 pKa = 8.41 HH72 pKa = 4.94 QRR74 pKa = 11.84 MVTRR78 pKa = 11.84 AIKK81 pKa = 10.06 RR82 pKa = 11.84 ARR84 pKa = 11.84 TIALMPYY91 pKa = 9.89 VADD94 pKa = 3.71
Molecular weight: 10.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.324
IPC2_protein 9.823
IPC_protein 10.701
Toseland 11.023
ProMoST 10.804
Dawson 11.082
Bjellqvist 10.818
Wikipedia 11.316
Rodwell 11.301
Grimsley 11.111
Solomon 11.272
Lehninger 11.228
Nozaki 11.008
DTASelect 10.804
Thurlkill 11.008
EMBOSS 11.433
Sillero 11.023
Patrickios 11.038
IPC_peptide 11.272
IPC2_peptide 9.926
IPC2.peptide.svr19 8.581
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3564
0
3564
1119672
38
2069
314.2
35.24
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.467 ± 0.047
1.171 ± 0.018
5.594 ± 0.034
7.432 ± 0.045
4.331 ± 0.031
6.706 ± 0.04
1.485 ± 0.017
9.148 ± 0.042
7.895 ± 0.037
8.726 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.064 ± 0.024
5.82 ± 0.028
3.027 ± 0.022
2.498 ± 0.018
3.363 ± 0.029
6.408 ± 0.029
5.21 ± 0.032
6.871 ± 0.032
0.7 ± 0.011
4.082 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here