Diaphorobacter sp. HDW4B

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Diaphorobacter; unclassified Diaphorobacter

Average proteome isoelectric point is 6.74

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5487 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G8C063|A0A6G8C063_9BURK Poly(A) polymerase I OS=Diaphorobacter sp. HDW4B OX=2714925 GN=pcnB PE=3 SV=1
MM1 pKa = 7.6ANDD4 pKa = 4.64TITIYY9 pKa = 10.76GAAPNHH15 pKa = 4.97NTGPKK20 pKa = 8.57VAYY23 pKa = 9.62EE24 pKa = 4.19VFTNQAVDD32 pKa = 3.55GVLVHH37 pKa = 6.91HH38 pKa = 6.36KK39 pKa = 9.27TVIYY43 pKa = 10.5TNAQQVSGVQLAVQPLVNNPPVDD66 pKa = 4.21PDD68 pKa = 3.89DD69 pKa = 3.99TNTAYY74 pKa = 9.88KK75 pKa = 10.51NATLTVSAANGVLNGASDD93 pKa = 4.66PDD95 pKa = 3.69TGDD98 pKa = 3.09ILSILWFQLPGDD110 pKa = 4.01TTVYY114 pKa = 10.88AAGQTANIAGVGTLQINADD133 pKa = 3.38GSYY136 pKa = 10.96VFTPAANYY144 pKa = 8.91VGPVPVSTYY153 pKa = 10.08TVTDD157 pKa = 3.84GNGGLDD163 pKa = 3.37TSTLTITMANNDD175 pKa = 3.8PPIDD179 pKa = 3.98GNEE182 pKa = 4.08VEE184 pKa = 4.75TTPQGTPISKK194 pKa = 10.1NVLSNATDD202 pKa = 3.73PQNDD206 pKa = 4.0PLSITGFVVGGTPYY220 pKa = 11.01NPGDD224 pKa = 3.61TATLPGVGTLLINADD239 pKa = 3.27GSYY242 pKa = 11.05VFTPLVTYY250 pKa = 9.68TGAVPQATYY259 pKa = 9.59TVSDD263 pKa = 3.21GHH265 pKa = 7.49GGVDD269 pKa = 3.81TSTLDD274 pKa = 3.19ITITPHH280 pKa = 5.94VPAAPASTPEE290 pKa = 4.09DD291 pKa = 3.55TPVPVTGVTIPPAGATVTTTVTVTNGTATVSTGGATVTGNGTSTITITGTPAQVNAALSGLTFTNTPDD359 pKa = 3.57YY360 pKa = 10.8NGPASVTITPDD371 pKa = 2.8NGAAPQTVPIPVTPVVDD388 pKa = 3.78VTDD391 pKa = 4.28DD392 pKa = 4.01ARR394 pKa = 11.84TTPLNTPVTIPVLANDD410 pKa = 3.74SFEE413 pKa = 4.14NPARR417 pKa = 11.84VISEE421 pKa = 3.97INGQAVTVGTPITVTNGTVTVQADD445 pKa = 3.4NTVIFTPDD453 pKa = 2.85KK454 pKa = 10.72DD455 pKa = 4.14YY456 pKa = 11.55TGTAQFTYY464 pKa = 8.94TVSAGGVKK472 pKa = 8.6EE473 pKa = 4.13TANVTVTVNGSTAAPTPVPVGGSLFGGLMVTLMAAWGMRR512 pKa = 11.84NLRR515 pKa = 11.84RR516 pKa = 11.84RR517 pKa = 11.84KK518 pKa = 9.03PP519 pKa = 3.26

Molecular weight: 52.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G8BX64|A0A6G8BX64_9BURK Tripartite tricarboxylate transporter substrate binding protein OS=Diaphorobacter sp. HDW4B OX=2714925 GN=G7048_12370 PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 7.79TRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.79GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5487

0

5487

1819837

32

4011

331.7

36.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.899 ± 0.041

0.957 ± 0.012

5.214 ± 0.024

5.271 ± 0.034

3.509 ± 0.022

8.106 ± 0.033

2.246 ± 0.019

4.654 ± 0.026

3.919 ± 0.03

10.094 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.693 ± 0.019

3.122 ± 0.023

5.022 ± 0.021

4.214 ± 0.024

6.035 ± 0.038

6.057 ± 0.03

5.569 ± 0.051

7.65 ± 0.03

1.495 ± 0.013

2.274 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski