Amino acid dipepetide frequency for Diaphorobacter sp. HDW4B

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.229AlaAla: 15.229 ± 0.148
1.231AlaCys: 1.231 ± 0.027
6.145AlaAsp: 6.145 ± 0.069
6.053AlaGlu: 6.053 ± 0.069
3.834AlaPhe: 3.834 ± 0.054
9.474AlaGly: 9.474 ± 0.085
2.56AlaHis: 2.56 ± 0.039
5.168AlaIle: 5.168 ± 0.058
4.763AlaLys: 4.763 ± 0.067
13.11AlaLeu: 13.11 ± 0.099
3.588AlaMet: 3.588 ± 0.049
3.439AlaAsn: 3.439 ± 0.055
5.597AlaPro: 5.597 ± 0.07
5.803AlaGln: 5.803 ± 0.069
7.127AlaArg: 7.127 ± 0.067
7.225AlaSer: 7.225 ± 0.078
6.174AlaThr: 6.174 ± 0.082
8.391AlaVal: 8.391 ± 0.083
1.674AlaTrp: 1.674 ± 0.033
2.408AlaTyr: 2.408 ± 0.041
0.0AlaXaa: 0.0 ± 0.0
Cys
1.139CysAla: 1.139 ± 0.025
0.113CysCys: 0.113 ± 0.008
0.55CysAsp: 0.55 ± 0.018
0.514CysGlu: 0.514 ± 0.015
0.321CysPhe: 0.321 ± 0.014
0.953CysGly: 0.953 ± 0.022
0.258CysHis: 0.258 ± 0.013
0.459CysIle: 0.459 ± 0.013
0.321CysLys: 0.321 ± 0.013
0.807CysLeu: 0.807 ± 0.019
0.27CysMet: 0.27 ± 0.013
0.271CysAsn: 0.271 ± 0.012
0.469CysPro: 0.469 ± 0.018
0.27CysGln: 0.27 ± 0.013
0.471CysArg: 0.471 ± 0.019
0.596CysSer: 0.596 ± 0.017
0.662CysThr: 0.662 ± 0.031
0.801CysVal: 0.801 ± 0.018
0.125CysTrp: 0.125 ± 0.008
0.198CysTyr: 0.198 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
6.698AspAla: 6.698 ± 0.073
0.453AspCys: 0.453 ± 0.015
2.738AspAsp: 2.738 ± 0.043
3.202AspGlu: 3.202 ± 0.046
2.018AspPhe: 2.018 ± 0.038
4.406AspGly: 4.406 ± 0.06
1.07AspHis: 1.07 ± 0.027
2.513AspIle: 2.513 ± 0.038
2.146AspLys: 2.146 ± 0.038
5.065AspLeu: 5.065 ± 0.054
1.455AspMet: 1.455 ± 0.029
1.434AspAsn: 1.434 ± 0.03
2.611AspPro: 2.611 ± 0.04
1.72AspGln: 1.72 ± 0.032
2.832AspArg: 2.832 ± 0.044
2.655AspSer: 2.655 ± 0.041
2.482AspThr: 2.482 ± 0.044
4.304AspVal: 4.304 ± 0.057
0.95AspTrp: 0.95 ± 0.022
1.386AspTyr: 1.386 ± 0.029
0.0AspXaa: 0.0 ± 0.0
Glu
5.901GluAla: 5.901 ± 0.078
0.461GluCys: 0.461 ± 0.015
2.317GluAsp: 2.317 ± 0.037
2.676GluGlu: 2.676 ± 0.047
1.891GluPhe: 1.891 ± 0.031
3.568GluGly: 3.568 ± 0.047
1.474GluHis: 1.474 ± 0.029
2.629GluIle: 2.629 ± 0.044
2.311GluLys: 2.311 ± 0.038
5.818GluLeu: 5.818 ± 0.067
1.35GluMet: 1.35 ± 0.029
1.476GluAsn: 1.476 ± 0.032
2.366GluPro: 2.366 ± 0.04
2.861GluGln: 2.861 ± 0.044
4.303GluArg: 4.303 ± 0.054
2.961GluSer: 2.961 ± 0.043
2.433GluThr: 2.433 ± 0.04
3.942GluVal: 3.942 ± 0.055
0.834GluTrp: 0.834 ± 0.021
1.137GluTyr: 1.137 ± 0.026
0.0GluXaa: 0.0 ± 0.0
Phe
4.108PheAla: 4.108 ± 0.049
0.401PheCys: 0.401 ± 0.017
2.374PheAsp: 2.374 ± 0.04
2.045PheGlu: 2.045 ± 0.036
1.337PhePhe: 1.337 ± 0.033
3.347PheGly: 3.347 ± 0.056
0.712PheHis: 0.712 ± 0.02
1.567PheIle: 1.567 ± 0.032
1.398PheLys: 1.398 ± 0.032
2.765PheLeu: 2.765 ± 0.041
0.886PheMet: 0.886 ± 0.025
1.27PheAsn: 1.27 ± 0.026
1.51PhePro: 1.51 ± 0.034
1.118PheGln: 1.118 ± 0.023
1.666PheArg: 1.666 ± 0.033
2.316PheSer: 2.316 ± 0.043
2.049PheThr: 2.049 ± 0.032
2.771PheVal: 2.771 ± 0.04
0.54PheTrp: 0.54 ± 0.019
0.912PheTyr: 0.912 ± 0.025
0.0PheXaa: 0.0 ± 0.0
Gly
8.708GlyAla: 8.708 ± 0.103
0.885GlyCys: 0.885 ± 0.023
3.949GlyAsp: 3.949 ± 0.049
4.239GlyGlu: 4.239 ± 0.056
3.155GlyPhe: 3.155 ± 0.044
6.848GlyGly: 6.848 ± 0.104
1.763GlyHis: 1.763 ± 0.037
4.121GlyIle: 4.121 ± 0.066
3.865GlyLys: 3.865 ± 0.053
7.948GlyLeu: 7.948 ± 0.085
2.53GlyMet: 2.53 ± 0.04
2.628GlyAsn: 2.628 ± 0.051
2.819GlyPro: 2.819 ± 0.046
3.207GlyGln: 3.207 ± 0.051
4.293GlyArg: 4.293 ± 0.05
5.094GlySer: 5.094 ± 0.066
4.763GlyThr: 4.763 ± 0.074
6.574GlyVal: 6.574 ± 0.073
1.406GlyTrp: 1.406 ± 0.033
2.263GlyTyr: 2.263 ± 0.037
0.0GlyXaa: 0.0 ± 0.0
His
2.726HisAla: 2.726 ± 0.048
0.304HisCys: 0.304 ± 0.011
1.16HisAsp: 1.16 ± 0.025
1.197HisGlu: 1.197 ± 0.026
0.915HisPhe: 0.915 ± 0.023
1.979HisGly: 1.979 ± 0.036
0.591HisHis: 0.591 ± 0.022
1.071HisIle: 1.071 ± 0.024
0.673HisLys: 0.673 ± 0.019
2.171HisLeu: 2.171 ± 0.035
0.615HisMet: 0.615 ± 0.018
0.553HisAsn: 0.553 ± 0.015
1.411HisPro: 1.411 ± 0.026
0.764HisGln: 0.764 ± 0.02
1.309HisArg: 1.309 ± 0.031
1.196HisSer: 1.196 ± 0.025
1.048HisThr: 1.048 ± 0.024
1.689HisVal: 1.689 ± 0.032
0.46HisTrp: 0.46 ± 0.017
0.624HisTyr: 0.624 ± 0.02
0.0HisXaa: 0.0 ± 0.0
Ile
6.48IleAla: 6.48 ± 0.064
0.48IleCys: 0.48 ± 0.017
3.089IleAsp: 3.089 ± 0.046
3.021IleGlu: 3.021 ± 0.045
1.391IlePhe: 1.391 ± 0.03
4.223IleGly: 4.223 ± 0.06
1.0IleHis: 1.0 ± 0.024
1.789IleIle: 1.789 ± 0.036
1.71IleLys: 1.71 ± 0.038
3.457IleLeu: 3.457 ± 0.044
0.872IleMet: 0.872 ± 0.024
1.627IleAsn: 1.627 ± 0.03
2.239IlePro: 2.239 ± 0.032
1.55IleGln: 1.55 ± 0.035
2.514IleArg: 2.514 ± 0.043
2.849IleSer: 2.849 ± 0.041
2.923IleThr: 2.923 ± 0.043
3.692IleVal: 3.692 ± 0.043
0.579IleTrp: 0.579 ± 0.019
1.056IleTyr: 1.056 ± 0.023
0.0IleXaa: 0.0 ± 0.0
Lys
4.7LysAla: 4.7 ± 0.057
0.181LysCys: 0.181 ± 0.011
2.106LysAsp: 2.106 ± 0.035
2.056LysGlu: 2.056 ± 0.038
1.122LysPhe: 1.122 ± 0.029
2.805LysGly: 2.805 ± 0.043
0.875LysHis: 0.875 ± 0.026
1.774LysIle: 1.774 ± 0.032
1.755LysLys: 1.755 ± 0.041
4.032LysLeu: 4.032 ± 0.05
0.965LysMet: 0.965 ± 0.025
1.335LysAsn: 1.335 ± 0.033
2.454LysPro: 2.454 ± 0.042
1.679LysGln: 1.679 ± 0.033
2.515LysArg: 2.515 ± 0.038
2.332LysSer: 2.332 ± 0.038
2.277LysThr: 2.277 ± 0.034
2.934LysVal: 2.934 ± 0.047
0.491LysTrp: 0.491 ± 0.018
0.799LysTyr: 0.799 ± 0.021
0.0LysXaa: 0.0 ± 0.0
Leu
12.571LeuAla: 12.571 ± 0.096
1.079LeuCys: 1.079 ± 0.027
5.26LeuAsp: 5.26 ± 0.066
4.984LeuGlu: 4.984 ± 0.064
3.198LeuPhe: 3.198 ± 0.051
8.047LeuGly: 8.047 ± 0.076
2.289LeuHis: 2.289 ± 0.04
4.623LeuIle: 4.623 ± 0.058
4.03LeuLys: 4.03 ± 0.054
10.179LeuLeu: 10.179 ± 0.11
2.614LeuMet: 2.614 ± 0.04
3.119LeuAsn: 3.119 ± 0.039
5.665LeuPro: 5.665 ± 0.068
4.369LeuGln: 4.369 ± 0.066
6.544LeuArg: 6.544 ± 0.073
6.46LeuSer: 6.46 ± 0.058
5.361LeuThr: 5.361 ± 0.069
7.321LeuVal: 7.321 ± 0.076
1.285LeuTrp: 1.285 ± 0.032
1.942LeuTyr: 1.942 ± 0.033
0.0LeuXaa: 0.0 ± 0.0
Met
3.1MetAla: 3.1 ± 0.042
0.208MetCys: 0.208 ± 0.011
1.242MetAsp: 1.242 ± 0.027
1.194MetGlu: 1.194 ± 0.029
0.821MetPhe: 0.821 ± 0.021
2.085MetGly: 2.085 ± 0.04
0.628MetHis: 0.628 ± 0.019
1.074MetIle: 1.074 ± 0.026
1.136MetLys: 1.136 ± 0.026
2.902MetLeu: 2.902 ± 0.046
0.67MetMet: 0.67 ± 0.021
0.991MetAsn: 0.991 ± 0.021
1.556MetPro: 1.556 ± 0.032
1.354MetGln: 1.354 ± 0.027
1.839MetArg: 1.839 ± 0.032
1.941MetSer: 1.941 ± 0.035
1.644MetThr: 1.644 ± 0.028
1.876MetVal: 1.876 ± 0.032
0.249MetTrp: 0.249 ± 0.011
0.425MetTyr: 0.425 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
4.101AsnAla: 4.101 ± 0.054
0.299AsnCys: 0.299 ± 0.015
1.586AsnAsp: 1.586 ± 0.029
1.473AsnGlu: 1.473 ± 0.027
1.073AsnPhe: 1.073 ± 0.026
2.83AsnGly: 2.83 ± 0.048
0.616AsnHis: 0.616 ± 0.018
1.435AsnIle: 1.435 ± 0.032
1.179AsnLys: 1.179 ± 0.028
2.745AsnLeu: 2.745 ± 0.038
0.746AsnMet: 0.746 ± 0.022
1.065AsnAsn: 1.065 ± 0.032
2.006AsnPro: 2.006 ± 0.037
1.073AsnGln: 1.073 ± 0.028
1.624AsnArg: 1.624 ± 0.031
1.663AsnSer: 1.663 ± 0.038
1.911AsnThr: 1.911 ± 0.043
2.467AsnVal: 2.467 ± 0.042
0.503AsnTrp: 0.503 ± 0.018
0.828AsnTyr: 0.828 ± 0.023
0.0AsnXaa: 0.0 ± 0.0
Pro
6.032ProAla: 6.032 ± 0.072
0.342ProCys: 0.342 ± 0.014
2.94ProAsp: 2.94 ± 0.052
3.287ProGlu: 3.287 ± 0.044
1.793ProPhe: 1.793 ± 0.036
4.042ProGly: 4.042 ± 0.055
1.138ProHis: 1.138 ± 0.031
2.073ProIle: 2.073 ± 0.035
1.817ProLys: 1.817 ± 0.039
4.844ProLeu: 4.844 ± 0.056
1.356ProMet: 1.356 ± 0.027
1.508ProAsn: 1.508 ± 0.033
2.222ProPro: 2.222 ± 0.046
2.253ProGln: 2.253 ± 0.036
2.515ProArg: 2.515 ± 0.037
3.061ProSer: 3.061 ± 0.052
2.857ProThr: 2.857 ± 0.052
4.21ProVal: 4.21 ± 0.052
0.73ProTrp: 0.73 ± 0.02
1.196ProTyr: 1.196 ± 0.027
0.0ProXaa: 0.0 ± 0.0
Gln
5.06GlnAla: 5.06 ± 0.065
0.351GlnCys: 0.351 ± 0.016
1.698GlnAsp: 1.698 ± 0.033
1.801GlnGlu: 1.801 ± 0.035
1.456GlnPhe: 1.456 ± 0.027
3.013GlnGly: 3.013 ± 0.045
1.034GlnHis: 1.034 ± 0.023
2.154GlnIle: 2.154 ± 0.037
1.491GlnLys: 1.491 ± 0.031
4.572GlnLeu: 4.572 ± 0.058
1.196GlnMet: 1.196 ± 0.027
1.132GlnAsn: 1.132 ± 0.025
2.217GlnPro: 2.217 ± 0.033
2.245GlnGln: 2.245 ± 0.063
3.217GlnArg: 3.217 ± 0.051
2.539GlnSer: 2.539 ± 0.045
2.247GlnThr: 2.247 ± 0.037
2.989GlnVal: 2.989 ± 0.045
0.844GlnTrp: 0.844 ± 0.022
0.888GlnTyr: 0.888 ± 0.024
0.0GlnXaa: 0.0 ± 0.0
Arg
6.441ArgAla: 6.441 ± 0.06
0.517ArgCys: 0.517 ± 0.019
3.23ArgAsp: 3.23 ± 0.042
3.766ArgGlu: 3.766 ± 0.048
2.458ArgPhe: 2.458 ± 0.044
3.971ArgGly: 3.971 ± 0.05
1.519ArgHis: 1.519 ± 0.029
3.324ArgIle: 3.324 ± 0.041
2.44ArgLys: 2.44 ± 0.046
6.27ArgLeu: 6.27 ± 0.076
1.928ArgMet: 1.928 ± 0.035
1.977ArgAsn: 1.977 ± 0.033
2.51ArgPro: 2.51 ± 0.042
2.404ArgGln: 2.404 ± 0.039
3.743ArgArg: 3.743 ± 0.057
3.498ArgSer: 3.498 ± 0.051
3.059ArgThr: 3.059 ± 0.043
4.597ArgVal: 4.597 ± 0.06
1.062ArgTrp: 1.062 ± 0.026
1.64ArgTyr: 1.64 ± 0.031
0.0ArgXaa: 0.0 ± 0.0
Ser
7.068SerAla: 7.068 ± 0.076
0.541SerCys: 0.541 ± 0.022
3.04SerAsp: 3.04 ± 0.038
2.854SerGlu: 2.854 ± 0.044
2.294SerPhe: 2.294 ± 0.038
5.797SerGly: 5.797 ± 0.064
1.258SerHis: 1.258 ± 0.028
2.913SerIle: 2.913 ± 0.044
2.177SerLys: 2.177 ± 0.039
5.87SerLeu: 5.87 ± 0.061
1.615SerMet: 1.615 ± 0.029
1.934SerAsn: 1.934 ± 0.038
2.988SerPro: 2.988 ± 0.044
2.27SerGln: 2.27 ± 0.038
3.45SerArg: 3.45 ± 0.052
3.99SerSer: 3.99 ± 0.056
3.7SerThr: 3.7 ± 0.055
4.566SerVal: 4.566 ± 0.062
0.835SerTrp: 0.835 ± 0.023
1.407SerTyr: 1.407 ± 0.032
0.0SerXaa: 0.0 ± 0.0
Thr
6.311ThrAla: 6.311 ± 0.087
0.529ThrCys: 0.529 ± 0.025
2.73ThrAsp: 2.73 ± 0.052
2.556ThrGlu: 2.556 ± 0.038
1.792ThrPhe: 1.792 ± 0.032
4.982ThrGly: 4.982 ± 0.098
1.258ThrHis: 1.258 ± 0.025
2.429ThrIle: 2.429 ± 0.045
1.738ThrLys: 1.738 ± 0.034
6.057ThrLeu: 6.057 ± 0.065
1.258ThrMet: 1.258 ± 0.027
1.674ThrAsn: 1.674 ± 0.046
3.766ThrPro: 3.766 ± 0.055
2.286ThrGln: 2.286 ± 0.04
3.094ThrArg: 3.094 ± 0.039
3.349ThrSer: 3.349 ± 0.051
3.491ThrThr: 3.491 ± 0.072
4.433ThrVal: 4.433 ± 0.092
0.726ThrTrp: 0.726 ± 0.023
1.236ThrTyr: 1.236 ± 0.034
0.0ThrXaa: 0.0 ± 0.0
Val
8.757ValAla: 8.757 ± 0.079
0.772ValCys: 0.772 ± 0.021
4.066ValAsp: 4.066 ± 0.06
3.986ValGlu: 3.986 ± 0.05
2.682ValPhe: 2.682 ± 0.044
5.63ValGly: 5.63 ± 0.077
1.644ValHis: 1.644 ± 0.032
3.795ValIle: 3.795 ± 0.046
2.804ValLys: 2.804 ± 0.044
8.003ValLeu: 8.003 ± 0.071
2.052ValMet: 2.052 ± 0.034
2.595ValAsn: 2.595 ± 0.048
4.028ValPro: 4.028 ± 0.053
3.247ValGln: 3.247 ± 0.04
4.713ValArg: 4.713 ± 0.053
4.51ValSer: 4.51 ± 0.052
4.466ValThr: 4.466 ± 0.081
6.249ValVal: 6.249 ± 0.062
0.989ValTrp: 0.989 ± 0.026
1.512ValTyr: 1.512 ± 0.032
0.0ValXaa: 0.0 ± 0.0
Trp
1.3TrpAla: 1.3 ± 0.029
0.175TrpCys: 0.175 ± 0.01
0.641TrpAsp: 0.641 ± 0.018
0.584TrpGlu: 0.584 ± 0.02
0.604TrpPhe: 0.604 ± 0.019
1.037TrpGly: 1.037 ± 0.027
0.357TrpHis: 0.357 ± 0.015
0.756TrpIle: 0.756 ± 0.022
0.58TrpLys: 0.58 ± 0.02
2.016TrpLeu: 2.016 ± 0.04
0.476TrpMet: 0.476 ± 0.02
0.519TrpAsn: 0.519 ± 0.015
0.674TrpPro: 0.674 ± 0.019
0.786TrpGln: 0.786 ± 0.021
1.07TrpArg: 1.07 ± 0.025
0.961TrpSer: 0.961 ± 0.025
0.8TrpThr: 0.8 ± 0.021
0.993TrpVal: 0.993 ± 0.023
0.295TrpTrp: 0.295 ± 0.013
0.322TrpTyr: 0.322 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.565TyrAla: 2.565 ± 0.037
0.247TyrCys: 0.247 ± 0.011
1.279TyrAsp: 1.279 ± 0.028
1.221TyrGlu: 1.221 ± 0.028
0.934TyrPhe: 0.934 ± 0.023
2.017TyrGly: 2.017 ± 0.032
0.415TyrHis: 0.415 ± 0.015
0.87TyrIle: 0.87 ± 0.022
0.855TyrLys: 0.855 ± 0.025
2.307TyrLeu: 2.307 ± 0.041
0.493TyrMet: 0.493 ± 0.015
0.679TyrAsn: 0.679 ± 0.023
1.109TyrPro: 1.109 ± 0.025
0.885TyrGln: 0.885 ± 0.025
1.513TyrArg: 1.513 ± 0.033
1.372TyrSer: 1.372 ± 0.03
1.35TyrThr: 1.35 ± 0.032
1.701TyrVal: 1.701 ± 0.031
0.37TyrTrp: 0.37 ± 0.015
0.563TyrTyr: 0.563 ± 0.019
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5487 proteins (1819837 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski