Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes; Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 219 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P21604|E2_VACCW Protein E2 OS=Vaccinia virus (strain Western Reserve) OX=10254 GN=VACWR058 PE=1 SV=2
MM1 pKa = 7.62SNQNIPQLSEE11 pKa = 3.95YY12 pKa = 8.12QTSVSQVAVTPPPKK26 pKa = 10.09PKK28 pKa = 9.48TPQIFEE34 pKa = 4.35YY35 pKa = 9.01QTSDD39 pKa = 3.82SIVNNPRR46 pKa = 11.84PFYY49 pKa = 11.08NSDD52 pKa = 3.98LEE54 pKa = 4.32FDD56 pKa = 5.37DD57 pKa = 4.16IDD59 pKa = 4.55MYY61 pKa = 11.59LLPNN65 pKa = 4.08

Molecular weight: 7.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P12924|I5_VACCW Protein I5 OS=Vaccinia virus (strain Western Reserve) OX=10254 GN=VACWR074 PE=1 SV=1
MM1 pKa = 7.52LNFSLCLYY9 pKa = 10.21PVFILNKK16 pKa = 9.97LVLRR20 pKa = 11.84TQSIILHH27 pKa = 6.43TINNASIKK35 pKa = 10.44NRR37 pKa = 3.61

Molecular weight: 4.3 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

217

2

219

57191

35

1287

261.1

30.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.885 ± 0.114

2.051 ± 0.084

6.693 ± 0.13

5.454 ± 0.14

4.662 ± 0.128

3.997 ± 0.101

2.063 ± 0.081

9.5 ± 0.156

7.4 ± 0.119

8.753 ± 0.131

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.836 ± 0.085

6.884 ± 0.134

3.415 ± 0.091

2.229 ± 0.078

4.321 ± 0.108

7.725 ± 0.132

6.08 ± 0.125

6.144 ± 0.114

0.733 ± 0.04

5.176 ± 0.121
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski