Amino acid dipepetide frequency for Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.465AlaAla: 2.465 ± 0.253
1.102AlaCys: 1.102 ± 0.149
2.098AlaAsp: 2.098 ± 0.177
1.871AlaGlu: 1.871 ± 0.221
1.818AlaPhe: 1.818 ± 0.162
1.486AlaGly: 1.486 ± 0.161
0.507AlaHis: 0.507 ± 0.09
3.62AlaIle: 3.62 ± 0.281
2.903AlaLys: 2.903 ± 0.241
3.427AlaLeu: 3.427 ± 0.245
1.067AlaMet: 1.067 ± 0.131
2.413AlaAsn: 2.413 ± 0.208
1.119AlaPro: 1.119 ± 0.14
0.699AlaGln: 0.699 ± 0.102
1.714AlaArg: 1.714 ± 0.183
3.357AlaSer: 3.357 ± 0.281
2.413AlaThr: 2.413 ± 0.221
2.973AlaVal: 2.973 ± 0.194
0.245AlaTrp: 0.245 ± 0.06
1.556AlaTyr: 1.556 ± 0.16
0.0AlaXaa: 0.0 ± 0.0
Cys
0.892CysAla: 0.892 ± 0.124
0.577CysCys: 0.577 ± 0.096
1.364CysAsp: 1.364 ± 0.156
0.962CysGlu: 0.962 ± 0.159
0.629CysPhe: 0.629 ± 0.092
0.944CysGly: 0.944 ± 0.101
0.455CysHis: 0.455 ± 0.084
2.081CysIle: 2.081 ± 0.202
1.207CysLys: 1.207 ± 0.145
1.609CysLeu: 1.609 ± 0.177
0.595CysMet: 0.595 ± 0.099
1.364CysAsn: 1.364 ± 0.178
0.682CysPro: 0.682 ± 0.115
0.437CysGln: 0.437 ± 0.1
0.909CysArg: 0.909 ± 0.111
1.626CysSer: 1.626 ± 0.187
1.259CysThr: 1.259 ± 0.161
1.504CysVal: 1.504 ± 0.179
0.245CysTrp: 0.245 ± 0.076
1.172CysTyr: 1.172 ± 0.149
0.0CysXaa: 0.0 ± 0.0
Asp
2.798AspAla: 2.798 ± 0.226
1.014AspCys: 1.014 ± 0.151
5.613AspAsp: 5.613 ± 0.365
4.511AspGlu: 4.511 ± 0.263
2.745AspPhe: 2.745 ± 0.206
3.042AspGly: 3.042 ± 0.229
1.189AspHis: 1.189 ± 0.125
7.851AspIle: 7.851 ± 0.401
5.246AspLys: 5.246 ± 0.332
4.774AspLeu: 4.774 ± 0.305
1.801AspMet: 1.801 ± 0.15
4.564AspAsn: 4.564 ± 0.317
1.679AspPro: 1.679 ± 0.135
1.224AspGln: 1.224 ± 0.132
2.256AspArg: 2.256 ± 0.209
4.179AspSer: 4.179 ± 0.235
3.637AspThr: 3.637 ± 0.296
4.721AspVal: 4.721 ± 0.277
0.542AspTrp: 0.542 ± 0.095
3.55AspTyr: 3.55 ± 0.268
0.0AspXaa: 0.0 ± 0.0
Glu
2.081GluAla: 2.081 ± 0.174
1.154GluCys: 1.154 ± 0.121
3.585GluAsp: 3.585 ± 0.254
3.2GluGlu: 3.2 ± 0.291
2.535GluPhe: 2.535 ± 0.204
1.591GluGly: 1.591 ± 0.21
1.172GluHis: 1.172 ± 0.136
4.739GluIle: 4.739 ± 0.302
3.62GluLys: 3.62 ± 0.236
5.421GluLeu: 5.421 ± 0.318
1.661GluMet: 1.661 ± 0.177
3.497GluAsn: 3.497 ± 0.248
1.818GluPro: 1.818 ± 0.215
1.504GluGln: 1.504 ± 0.177
2.658GluArg: 2.658 ± 0.302
4.057GluSer: 4.057 ± 0.33
3.427GluThr: 3.427 ± 0.261
2.553GluVal: 2.553 ± 0.226
0.542GluTrp: 0.542 ± 0.079
3.724GluTyr: 3.724 ± 0.27
0.0GluXaa: 0.0 ± 0.0
Phe
1.714PheAla: 1.714 ± 0.206
0.857PheCys: 0.857 ± 0.137
3.217PheAsp: 3.217 ± 0.243
2.151PheGlu: 2.151 ± 0.171
2.151PhePhe: 2.151 ± 0.185
2.028PheGly: 2.028 ± 0.182
0.664PheHis: 0.664 ± 0.1
4.721PheIle: 4.721 ± 0.259
3.427PheLys: 3.427 ± 0.234
4.092PheLeu: 4.092 ± 0.307
1.574PheMet: 1.574 ± 0.137
3.462PheAsn: 3.462 ± 0.253
1.346PhePro: 1.346 ± 0.172
0.839PheGln: 0.839 ± 0.122
1.888PheArg: 1.888 ± 0.183
3.654PheSer: 3.654 ± 0.303
2.973PheThr: 2.973 ± 0.252
3.112PheVal: 3.112 ± 0.236
0.385PheTrp: 0.385 ± 0.084
2.343PheTyr: 2.343 ± 0.2
0.0PheXaa: 0.0 ± 0.0
Gly
1.993GlyAla: 1.993 ± 0.187
0.734GlyCys: 0.734 ± 0.123
2.448GlyAsp: 2.448 ± 0.197
2.116GlyGlu: 2.116 ± 0.181
1.853GlyPhe: 1.853 ± 0.178
2.238GlyGly: 2.238 ± 0.254
0.857GlyHis: 0.857 ± 0.104
3.987GlyIle: 3.987 ± 0.25
3.077GlyLys: 3.077 ± 0.275
3.06GlyLeu: 3.06 ± 0.23
0.979GlyMet: 0.979 ± 0.137
2.903GlyAsn: 2.903 ± 0.209
0.909GlyPro: 0.909 ± 0.14
0.647GlyGln: 0.647 ± 0.113
1.923GlyArg: 1.923 ± 0.185
2.868GlySer: 2.868 ± 0.215
2.203GlyThr: 2.203 ± 0.201
2.658GlyVal: 2.658 ± 0.247
0.28GlyTrp: 0.28 ± 0.065
2.238GlyTyr: 2.238 ± 0.245
0.0GlyXaa: 0.0 ± 0.0
His
0.892HisAla: 0.892 ± 0.129
0.56HisCys: 0.56 ± 0.113
1.207HisAsp: 1.207 ± 0.141
0.944HisGlu: 0.944 ± 0.139
0.804HisPhe: 0.804 ± 0.129
1.137HisGly: 1.137 ± 0.131
0.49HisHis: 0.49 ± 0.117
2.221HisIle: 2.221 ± 0.17
1.381HisLys: 1.381 ± 0.149
1.958HisLeu: 1.958 ± 0.155
0.629HisMet: 0.629 ± 0.102
1.049HisAsn: 1.049 ± 0.148
0.857HisPro: 0.857 ± 0.113
0.629HisGln: 0.629 ± 0.105
0.874HisArg: 0.874 ± 0.113
1.329HisSer: 1.329 ± 0.138
1.329HisThr: 1.329 ± 0.168
1.346HisVal: 1.346 ± 0.181
0.175HisTrp: 0.175 ± 0.05
0.822HisTyr: 0.822 ± 0.112
0.0HisXaa: 0.0 ± 0.0
Ile
3.357IleAla: 3.357 ± 0.234
1.556IleCys: 1.556 ± 0.147
7.257IleAsp: 7.257 ± 0.362
5.141IleGlu: 5.141 ± 0.287
4.232IlePhe: 4.232 ± 0.286
3.742IleGly: 3.742 ± 0.224
2.063IleHis: 2.063 ± 0.187
8.288IleIle: 8.288 ± 0.459
7.449IleLys: 7.449 ± 0.353
8.166IleLeu: 8.166 ± 0.419
2.203IleMet: 2.203 ± 0.202
7.746IleAsn: 7.746 ± 0.349
3.602IlePro: 3.602 ± 0.282
2.011IleGln: 2.011 ± 0.165
4.074IleArg: 4.074 ± 0.29
8.288IleSer: 8.288 ± 0.432
5.246IleThr: 5.246 ± 0.314
5.788IleVal: 5.788 ± 0.336
0.542IleTrp: 0.542 ± 0.093
4.249IleTyr: 4.249 ± 0.29
0.0IleXaa: 0.0 ± 0.0
Lys
2.081LysAla: 2.081 ± 0.198
1.679LysCys: 1.679 ± 0.178
4.756LysAsp: 4.756 ± 0.311
3.987LysGlu: 3.987 ± 0.249
3.217LysPhe: 3.217 ± 0.246
2.168LysGly: 2.168 ± 0.159
1.853LysHis: 1.853 ± 0.167
6.837LysIle: 6.837 ± 0.363
5.805LysLys: 5.805 ± 0.343
6.907LysLeu: 6.907 ± 0.331
2.046LysMet: 2.046 ± 0.175
5.228LysAsn: 5.228 ± 0.296
2.221LysPro: 2.221 ± 0.172
2.151LysGln: 2.151 ± 0.178
3.689LysArg: 3.689 ± 0.234
5.508LysSer: 5.508 ± 0.301
4.511LysThr: 4.511 ± 0.288
4.039LysVal: 4.039 ± 0.278
0.752LysTrp: 0.752 ± 0.131
4.564LysTyr: 4.564 ± 0.294
0.0LysXaa: 0.0 ± 0.0
Leu
3.287LeuAla: 3.287 ± 0.253
1.731LeuCys: 1.731 ± 0.196
5.788LeuAsp: 5.788 ± 0.356
5.176LeuGlu: 5.176 ± 0.342
4.931LeuPhe: 4.931 ± 0.366
3.147LeuGly: 3.147 ± 0.282
1.679LeuHis: 1.679 ± 0.241
6.575LeuIle: 6.575 ± 0.334
6.067LeuLys: 6.067 ± 0.33
8.918LeuLeu: 8.918 ± 0.47
2.378LeuMet: 2.378 ± 0.212
5.368LeuAsn: 5.368 ± 0.363
3.497LeuPro: 3.497 ± 0.232
1.871LeuGln: 1.871 ± 0.184
3.445LeuArg: 3.445 ± 0.284
7.676LeuSer: 7.676 ± 0.391
5.543LeuThr: 5.543 ± 0.349
5.508LeuVal: 5.508 ± 0.285
0.49LeuTrp: 0.49 ± 0.108
4.459LeuTyr: 4.459 ± 0.278
0.0LeuXaa: 0.0 ± 0.0
Met
1.556MetAla: 1.556 ± 0.161
0.525MetCys: 0.525 ± 0.093
2.133MetAsp: 2.133 ± 0.206
1.574MetGlu: 1.574 ± 0.147
1.434MetPhe: 1.434 ± 0.162
0.962MetGly: 0.962 ± 0.141
0.49MetHis: 0.49 ± 0.087
2.553MetIle: 2.553 ± 0.208
1.749MetLys: 1.749 ± 0.173
2.623MetLeu: 2.623 ± 0.211
0.997MetMet: 0.997 ± 0.138
1.766MetAsn: 1.766 ± 0.154
0.944MetPro: 0.944 ± 0.124
0.56MetGln: 0.56 ± 0.094
1.189MetArg: 1.189 ± 0.145
2.343MetSer: 2.343 ± 0.216
1.661MetThr: 1.661 ± 0.17
1.521MetVal: 1.521 ± 0.159
0.245MetTrp: 0.245 ± 0.076
1.539MetTyr: 1.539 ± 0.137
0.0MetXaa: 0.0 ± 0.0
Asn
2.885AsnAla: 2.885 ± 0.216
1.172AsnCys: 1.172 ± 0.154
4.546AsnAsp: 4.546 ± 0.308
3.585AsnGlu: 3.585 ± 0.269
2.745AsnPhe: 2.745 ± 0.228
3.182AsnGly: 3.182 ± 0.247
1.521AsnHis: 1.521 ± 0.151
7.501AsnIle: 7.501 ± 0.355
5.788AsnLys: 5.788 ± 0.305
4.948AsnLeu: 4.948 ± 0.331
2.168AsnMet: 2.168 ± 0.173
5.578AsnAsn: 5.578 ± 0.317
2.5AsnPro: 2.5 ± 0.219
1.311AsnGln: 1.311 ± 0.152
2.885AsnArg: 2.885 ± 0.223
4.406AsnSer: 4.406 ± 0.263
4.354AsnThr: 4.354 ± 0.278
4.424AsnVal: 4.424 ± 0.292
0.402AsnTrp: 0.402 ± 0.082
2.938AsnTyr: 2.938 ± 0.211
0.0AsnXaa: 0.0 ± 0.0
Pro
1.329ProAla: 1.329 ± 0.158
0.542ProCys: 0.542 ± 0.112
1.958ProAsp: 1.958 ± 0.168
2.413ProGlu: 2.413 ± 0.199
1.521ProPhe: 1.521 ± 0.159
1.311ProGly: 1.311 ± 0.154
0.752ProHis: 0.752 ± 0.132
3.077ProIle: 3.077 ± 0.25
2.221ProLys: 2.221 ± 0.186
2.85ProLeu: 2.85 ± 0.242
0.909ProMet: 0.909 ± 0.137
2.256ProAsn: 2.256 ± 0.191
1.644ProPro: 1.644 ± 0.178
0.752ProGln: 0.752 ± 0.111
1.609ProArg: 1.609 ± 0.216
2.675ProSer: 2.675 ± 0.204
2.308ProThr: 2.308 ± 0.202
2.151ProVal: 2.151 ± 0.186
0.28ProTrp: 0.28 ± 0.069
1.591ProTyr: 1.591 ± 0.165
0.0ProXaa: 0.0 ± 0.0
Gln
0.542GlnAla: 0.542 ± 0.108
0.56GlnCys: 0.56 ± 0.106
1.329GlnAsp: 1.329 ± 0.149
1.172GlnGlu: 1.172 ± 0.164
0.909GlnPhe: 0.909 ± 0.113
0.682GlnGly: 0.682 ± 0.157
0.664GlnHis: 0.664 ± 0.112
1.609GlnIle: 1.609 ± 0.154
1.521GlnLys: 1.521 ± 0.167
2.518GlnLeu: 2.518 ± 0.198
0.682GlnMet: 0.682 ± 0.098
1.329GlnAsn: 1.329 ± 0.146
0.734GlnPro: 0.734 ± 0.134
0.857GlnGln: 0.857 ± 0.135
1.224GlnArg: 1.224 ± 0.151
1.696GlnSer: 1.696 ± 0.17
1.504GlnThr: 1.504 ± 0.187
0.962GlnVal: 0.962 ± 0.119
0.262GlnTrp: 0.262 ± 0.067
1.539GlnTyr: 1.539 ± 0.147
0.0GlnXaa: 0.0 ± 0.0
Arg
1.259ArgAla: 1.259 ± 0.159
1.032ArgCys: 1.032 ± 0.166
2.885ArgAsp: 2.885 ± 0.254
2.413ArgGlu: 2.413 ± 0.322
2.133ArgPhe: 2.133 ± 0.187
1.906ArgGly: 1.906 ± 0.192
1.294ArgHis: 1.294 ± 0.158
3.445ArgIle: 3.445 ± 0.223
2.518ArgLys: 2.518 ± 0.21
4.197ArgLeu: 4.197 ± 0.246
1.119ArgMet: 1.119 ± 0.161
3.165ArgAsn: 3.165 ± 0.24
1.259ArgPro: 1.259 ± 0.164
1.399ArgGln: 1.399 ± 0.164
2.553ArgArg: 2.553 ± 0.23
3.06ArgSer: 3.06 ± 0.259
2.238ArgThr: 2.238 ± 0.18
2.396ArgVal: 2.396 ± 0.206
0.367ArgTrp: 0.367 ± 0.077
2.57ArgTyr: 2.57 ± 0.221
0.0ArgXaa: 0.0 ± 0.0
Ser
2.92SerAla: 2.92 ± 0.253
1.504SerCys: 1.504 ± 0.168
4.826SerAsp: 4.826 ± 0.35
3.532SerGlu: 3.532 ± 0.265
3.672SerPhe: 3.672 ± 0.288
3.375SerGly: 3.375 ± 0.252
1.416SerHis: 1.416 ± 0.153
7.571SerIle: 7.571 ± 0.346
6.242SerLys: 6.242 ± 0.359
6.732SerLeu: 6.732 ± 0.326
2.396SerMet: 2.396 ± 0.238
4.791SerAsn: 4.791 ± 0.32
3.008SerPro: 3.008 ± 0.259
1.976SerGln: 1.976 ± 0.205
3.235SerArg: 3.235 ± 0.213
6.505SerSer: 6.505 ± 0.38
4.896SerThr: 4.896 ± 0.304
4.774SerVal: 4.774 ± 0.255
0.385SerTrp: 0.385 ± 0.093
3.497SerTyr: 3.497 ± 0.264
0.0SerXaa: 0.0 ± 0.0
Thr
2.256ThrAla: 2.256 ± 0.193
1.346ThrCys: 1.346 ± 0.155
4.214ThrAsp: 4.214 ± 0.299
3.305ThrGlu: 3.305 ± 0.268
2.85ThrPhe: 2.85 ± 0.213
2.518ThrGly: 2.518 ± 0.215
1.241ThrHis: 1.241 ± 0.156
6.417ThrIle: 6.417 ± 0.337
4.301ThrLys: 4.301 ± 0.25
4.913ThrLeu: 4.913 ± 0.309
1.766ThrMet: 1.766 ± 0.189
3.672ThrAsn: 3.672 ± 0.244
2.535ThrPro: 2.535 ± 0.271
1.032ThrGln: 1.032 ± 0.148
2.413ThrArg: 2.413 ± 0.182
4.809ThrSer: 4.809 ± 0.286
3.899ThrThr: 3.899 ± 0.343
3.934ThrVal: 3.934 ± 0.302
0.525ThrTrp: 0.525 ± 0.091
2.85ThrTyr: 2.85 ± 0.197
0.0ThrXaa: 0.0 ± 0.0
Val
2.378ValAla: 2.378 ± 0.177
1.416ValCys: 1.416 ± 0.169
4.301ValAsp: 4.301 ± 0.268
3.742ValGlu: 3.742 ± 0.277
3.287ValPhe: 3.287 ± 0.276
1.784ValGly: 1.784 ± 0.174
1.119ValHis: 1.119 ± 0.158
5.665ValIle: 5.665 ± 0.307
4.983ValLys: 4.983 ± 0.29
4.878ValLeu: 4.878 ± 0.33
1.556ValMet: 1.556 ± 0.148
4.354ValAsn: 4.354 ± 0.278
1.941ValPro: 1.941 ± 0.187
1.224ValGln: 1.224 ± 0.143
2.57ValArg: 2.57 ± 0.241
4.913ValSer: 4.913 ± 0.304
3.969ValThr: 3.969 ± 0.278
3.654ValVal: 3.654 ± 0.303
0.262ValTrp: 0.262 ± 0.072
3.445ValTyr: 3.445 ± 0.263
0.0ValXaa: 0.0 ± 0.0
Trp
0.227TrpAla: 0.227 ± 0.056
0.175TrpCys: 0.175 ± 0.054
0.28TrpAsp: 0.28 ± 0.066
0.472TrpGlu: 0.472 ± 0.107
0.437TrpPhe: 0.437 ± 0.092
0.297TrpGly: 0.297 ± 0.064
0.122TrpHis: 0.122 ± 0.044
0.717TrpIle: 0.717 ± 0.136
0.734TrpLys: 0.734 ± 0.107
0.752TrpLeu: 0.752 ± 0.125
0.367TrpMet: 0.367 ± 0.076
0.49TrpAsn: 0.49 ± 0.103
0.227TrpPro: 0.227 ± 0.064
0.14TrpGln: 0.14 ± 0.046
0.245TrpArg: 0.245 ± 0.071
0.507TrpSer: 0.507 ± 0.092
0.455TrpThr: 0.455 ± 0.088
0.332TrpVal: 0.332 ± 0.079
0.0TrpTrp: 0.0 ± 0.0
0.35TrpTyr: 0.35 ± 0.077
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.941TyrAla: 1.941 ± 0.195
1.276TyrCys: 1.276 ± 0.145
3.13TyrAsp: 3.13 ± 0.199
2.273TyrGlu: 2.273 ± 0.198
2.71TyrPhe: 2.71 ± 0.202
2.43TyrGly: 2.43 ± 0.224
1.084TyrHis: 1.084 ± 0.126
5.525TyrIle: 5.525 ± 0.296
3.759TyrLys: 3.759 ± 0.253
4.791TyrLeu: 4.791 ± 0.278
1.451TyrMet: 1.451 ± 0.163
3.847TyrAsn: 3.847 ± 0.351
1.626TyrPro: 1.626 ± 0.153
1.032TyrGln: 1.032 ± 0.147
1.853TyrArg: 1.853 ± 0.212
3.794TyrSer: 3.794 ± 0.223
2.973TyrThr: 2.973 ± 0.249
3.095TyrVal: 3.095 ± 0.219
0.402TyrTrp: 0.402 ± 0.072
2.763TyrTyr: 2.763 ± 0.197
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 219 proteins (57191 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski