Fischerella thermalis
Average proteome isoelectric point is 6.53
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4557 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|G6FWV6|G6FWV6_9CYAN PapD_N domain-containing protein OS=Fischerella thermalis OX=372787 GN=FJSC11DRAFT_3355 PE=4 SV=1
MM1 pKa = 6.94 TVRR4 pKa = 11.84 VHH6 pKa = 6.89 FLPDD10 pKa = 3.72 DD11 pKa = 3.87 VTVDD15 pKa = 3.59 AQVGEE20 pKa = 4.33 PLLDD24 pKa = 3.37 VADD27 pKa = 4.33 RR28 pKa = 11.84 AEE30 pKa = 4.26 VIIPTGCLMGTCHH43 pKa = 7.06 ACTVEE48 pKa = 4.51 LDD50 pKa = 3.48 DD51 pKa = 5.46 GEE53 pKa = 4.71 IIRR56 pKa = 11.84 ACISSVPDD64 pKa = 3.29 GCEE67 pKa = 3.39 EE68 pKa = 3.79 LTINLFSDD76 pKa = 4.03 PTWW79 pKa = 4.29
Molecular weight: 8.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.737
IPC2_protein 3.834
IPC_protein 3.77
Toseland 3.567
ProMoST 3.948
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.719
Rodwell 3.605
Grimsley 3.49
Solomon 3.745
Lehninger 3.706
Nozaki 3.897
DTASelect 4.113
Thurlkill 3.643
EMBOSS 3.732
Sillero 3.897
Patrickios 0.769
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.791
Protein with the highest isoelectric point:
>tr|G6FNJ6|G6FNJ6_9CYAN TonB-dependent receptor OS=Fischerella thermalis OX=372787 GN=FJSC11DRAFT_0443 PE=3 SV=1
MM1 pKa = 6.99 SQSSSKK7 pKa = 10.71 LNRR10 pKa = 11.84 RR11 pKa = 11.84 LTQAVGIVLGITIAVWVLRR30 pKa = 11.84 GFGVLTFLPGGIILMLLLVAIAIGILSHH58 pKa = 6.6 LQKK61 pKa = 9.66 TWWRR65 pKa = 11.84 FF66 pKa = 3.05
Molecular weight: 7.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.347
IPC2_protein 10.73
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.486
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.193
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 11.974
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.106
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4557
0
4557
1410942
30
2376
309.6
34.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.223 ± 0.044
1.02 ± 0.012
4.674 ± 0.029
6.13 ± 0.043
3.934 ± 0.027
6.606 ± 0.034
1.895 ± 0.021
6.788 ± 0.028
4.854 ± 0.031
10.976 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.906 ± 0.015
4.296 ± 0.03
4.81 ± 0.03
5.505 ± 0.034
5.162 ± 0.034
6.142 ± 0.03
5.736 ± 0.029
6.866 ± 0.026
1.422 ± 0.018
3.053 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here