Bartonella apis
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2111 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1R0F9A8|A0A1R0F9A8_9RHIZ ApeA_NTD1 domain-containing protein OS=Bartonella apis OX=1686310 GN=PEB0149_009730 PE=4 SV=1
MM1 pKa = 7.71 LSPTSFDD8 pKa = 3.78 EE9 pKa = 4.85 INFLMQYY16 pKa = 10.45 SFYY19 pKa = 10.99 DD20 pKa = 2.89 AWKK23 pKa = 10.83 YY24 pKa = 10.62 NIQEE28 pKa = 4.03 LRR30 pKa = 11.84 LYY32 pKa = 10.4 DD33 pKa = 3.54 QLLAIDD39 pKa = 4.42 NVAPINLDD47 pKa = 2.93 GDD49 pKa = 5.06 FIRR52 pKa = 11.84 MPLPIRR58 pKa = 11.84 VLVFTFLSYY67 pKa = 11.38
Molecular weight: 8.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.857
IPC2_protein 4.177
IPC_protein 4.012
Toseland 3.808
ProMoST 4.215
Dawson 4.024
Bjellqvist 4.19
Wikipedia 4.012
Rodwell 3.846
Grimsley 3.732
Solomon 3.999
Lehninger 3.961
Nozaki 4.164
DTASelect 4.406
Thurlkill 3.897
EMBOSS 4.012
Sillero 4.139
Patrickios 1.977
IPC_peptide 3.999
IPC2_peptide 4.113
IPC2.peptide.svr19 4.032
Protein with the highest isoelectric point:
>tr|A0A1R0FBQ4|A0A1R0FBQ4_9RHIZ Propionyl-CoA carboxylase beta chain OS=Bartonella apis OX=1686310 GN=PEB0149_018730 PE=4 SV=1
MM1 pKa = 7.01 QVPLIRR7 pKa = 11.84 NPLKK11 pKa = 9.88 TPKK14 pKa = 9.51 IVPFIFVRR22 pKa = 11.84 KK23 pKa = 8.76 KK24 pKa = 10.89 CGVTTQGILAIGQYY38 pKa = 10.31 RR39 pKa = 11.84 FPCALGRR46 pKa = 11.84 NGLTTRR52 pKa = 11.84 KK53 pKa = 9.73 RR54 pKa = 11.84 EE55 pKa = 4.08 GDD57 pKa = 3.61 GATPIAVMPILGGFKK72 pKa = 10.6 KK73 pKa = 10.72 NNFPNLPHH81 pKa = 6.94 HH82 pKa = 6.47 LLKK85 pKa = 10.65 LHH87 pKa = 6.32 HH88 pKa = 6.66 VKK90 pKa = 10.6 PSDD93 pKa = 3.48 GWCDD97 pKa = 3.58 QSWDD101 pKa = 3.59 TNYY104 pKa = 10.12 NRR106 pKa = 11.84 PVRR109 pKa = 11.84 LPYY112 pKa = 10.36 KK113 pKa = 10.49 NSAEE117 pKa = 4.05 RR118 pKa = 11.84 LCRR121 pKa = 11.84 RR122 pKa = 11.84 DD123 pKa = 3.57 DD124 pKa = 4.1 LYY126 pKa = 11.38 DD127 pKa = 3.29 IVLIPDD133 pKa = 3.31 WNIRR137 pKa = 11.84 RR138 pKa = 11.84 RR139 pKa = 11.84 IQNCGSAIFFHH150 pKa = 6.96 LARR153 pKa = 11.84 PDD155 pKa = 3.81 FSPTEE160 pKa = 3.76 GCIAISRR167 pKa = 11.84 RR168 pKa = 11.84 TMEE171 pKa = 5.24 RR172 pKa = 11.84 ILPFISRR179 pKa = 11.84 EE180 pKa = 4.0 TKK182 pKa = 10.63 LITLGSITRR191 pKa = 11.84 SQPALVTRR199 pKa = 4.85
Molecular weight: 22.74 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.349
IPC2_protein 9.56
IPC_protein 10.101
Toseland 10.613
ProMoST 10.379
Dawson 10.716
Bjellqvist 10.394
Wikipedia 10.891
Rodwell 10.979
Grimsley 10.745
Solomon 10.804
Lehninger 10.774
Nozaki 10.628
DTASelect 10.379
Thurlkill 10.613
EMBOSS 11.008
Sillero 10.643
Patrickios 10.701
IPC_peptide 10.804
IPC2_peptide 9.56
IPC2.peptide.svr19 8.594
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2111
0
2111
691305
12
6082
327.5
36.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.402 ± 0.062
0.838 ± 0.019
5.794 ± 0.061
6.068 ± 0.051
4.307 ± 0.041
7.629 ± 0.07
2.142 ± 0.023
6.707 ± 0.048
5.888 ± 0.043
9.63 ± 0.077
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.523 ± 0.028
4.04 ± 0.052
4.39 ± 0.038
3.177 ± 0.028
5.418 ± 0.052
6.015 ± 0.038
5.313 ± 0.058
6.959 ± 0.045
1.105 ± 0.02
2.655 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here