Flaviflexus sp. H23T48
Average proteome isoelectric point is 5.71
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2334 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3Q9G680|A0A3Q9G680_9ACTO ABC transporter ATP-binding protein OS=Flaviflexus sp. H23T48 OX=2496867 GN=EJ997_01705 PE=4 SV=1
MM1 pKa = 7.26 TSHH4 pKa = 6.47 VPGSRR9 pKa = 11.84 RR10 pKa = 11.84 TLRR13 pKa = 11.84 ACIATLGAGALGLGAFIVPAAATPSADD40 pKa = 4.13 PIYY43 pKa = 10.6 EE44 pKa = 4.15 SDD46 pKa = 3.54 PSVLVGSSANQASVAGSAYY65 pKa = 10.01 GSRR68 pKa = 11.84 ATGEE72 pKa = 4.08 WFVSFSSAPLVNGGTQARR90 pKa = 11.84 VDD92 pKa = 3.36 ADD94 pKa = 3.99 LNEE97 pKa = 4.52 LEE99 pKa = 4.87 SAIADD104 pKa = 3.57 LDD106 pKa = 4.17 VEE108 pKa = 5.0 VKK110 pKa = 10.61 ASHH113 pKa = 6.01 SEE115 pKa = 3.69 LWAGAVVTADD125 pKa = 3.6 DD126 pKa = 4.24 AEE128 pKa = 4.33 LAKK131 pKa = 11.03 VLDD134 pKa = 4.21 RR135 pKa = 11.84 VDD137 pKa = 3.39 VAGVFPVLLIDD148 pKa = 4.75 APEE151 pKa = 3.79 QRR153 pKa = 11.84 DD154 pKa = 3.61 LGAEE158 pKa = 3.88 PEE160 pKa = 4.08 MRR162 pKa = 11.84 FAAGMTGADD171 pKa = 3.75 FAQDD175 pKa = 3.97 LGVSGYY181 pKa = 10.18 GQTISIIDD189 pKa = 3.46 TGVDD193 pKa = 2.93 IDD195 pKa = 4.0 HH196 pKa = 6.49 VAFGGTGVADD206 pKa = 3.62 TTPWSEE212 pKa = 3.82 INGNGSRR219 pKa = 11.84 IIGGYY224 pKa = 10.58 DD225 pKa = 3.71 FVGDD229 pKa = 4.72 DD230 pKa = 3.86 YY231 pKa = 11.65 NANPAADD238 pKa = 3.97 NYY240 pKa = 10.91 NPVPAPDD247 pKa = 4.75 SNPDD251 pKa = 3.3 DD252 pKa = 4.22 CQGHH256 pKa = 5.16 GTHH259 pKa = 6.15 VAGIAAGSDD268 pKa = 3.64 DD269 pKa = 3.63 VTGVYY274 pKa = 10.32 GVAPDD279 pKa = 4.24 ANILAYY285 pKa = 10.02 RR286 pKa = 11.84 VFGCEE291 pKa = 3.62 GSTDD295 pKa = 3.66 SAIMLQAMEE304 pKa = 4.23 ASARR308 pKa = 11.84 DD309 pKa = 3.35 GADD312 pKa = 2.78 IVNMSIGAGFMTWPNYY328 pKa = 5.66 PTAVAADD335 pKa = 3.82 NLVDD339 pKa = 3.76 AGVVVTVSQGNEE351 pKa = 3.9 GEE353 pKa = 4.37 SGVFSGGAPAVASKK367 pKa = 10.4 VISVGSVDD375 pKa = 3.76 NVASEE380 pKa = 4.43 APVFVVNDD388 pKa = 3.23 QNIAYY393 pKa = 9.52 SSSTGAPNPPTDD405 pKa = 3.32 NSEE408 pKa = 4.29 YY409 pKa = 10.85 EE410 pKa = 4.3 IIAYY414 pKa = 7.91 PAGSEE419 pKa = 4.01 TGAVPIEE426 pKa = 4.23 DD427 pKa = 4.1 AEE429 pKa = 4.49 GKK431 pKa = 9.85 VVIVRR436 pKa = 11.84 RR437 pKa = 11.84 GNSTFHH443 pKa = 6.6 EE444 pKa = 4.49 KK445 pKa = 9.97 AAAAQEE451 pKa = 4.21 SGAAGVIIDD460 pKa = 4.35 NNVAGAINATVEE472 pKa = 4.05 GDD474 pKa = 3.58 PAITIPTVTISMADD488 pKa = 3.21 GDD490 pKa = 5.16 AIRR493 pKa = 11.84 ADD495 pKa = 3.43 LAAAEE500 pKa = 4.43 EE501 pKa = 4.76 PVTLTWSDD509 pKa = 3.31 QTEE512 pKa = 4.1 VSEE515 pKa = 4.25 IATGGLVSDD524 pKa = 4.72 FSSYY528 pKa = 11.09 GLSADD533 pKa = 3.82 LTVKK537 pKa = 9.97 PQVVAPGGFINSAYY551 pKa = 9.16 PLEE554 pKa = 4.9 AGPSGYY560 pKa = 8.93 ATLSGTSMAAPHH572 pKa = 5.79 VAGAAALILEE582 pKa = 4.83 VNPALNPSQVLTSLQNTADD601 pKa = 3.86 PLLWSGNPDD610 pKa = 3.2 LGFPEE615 pKa = 4.48 PVHH618 pKa = 6.07 RR619 pKa = 11.84 QGAGMINIPNAIFANLWGTQVTPSQINLYY648 pKa = 10.96 DD649 pKa = 3.83 NDD651 pKa = 3.88 TGASEE656 pKa = 4.21 THH658 pKa = 6.3 TLTIHH663 pKa = 6.26 NRR665 pKa = 11.84 DD666 pKa = 3.44 EE667 pKa = 4.56 VEE669 pKa = 4.06 FTYY672 pKa = 11.1 SFVNLAGIATAGINQLPNYY691 pKa = 8.87 YY692 pKa = 9.94 GAAATTEE699 pKa = 4.66 FSAEE703 pKa = 4.25 TVTVPAGGTATVDD716 pKa = 3.36 VTITEE721 pKa = 4.34 PEE723 pKa = 4.25 GFAGGIYY730 pKa = 10.16 GGQIVVLGEE739 pKa = 4.08 DD740 pKa = 3.12 ASGYY744 pKa = 4.54 TTQSIVSYY752 pKa = 10.67 GGLAGDD758 pKa = 4.59 YY759 pKa = 7.32 EE760 pKa = 4.44 TDD762 pKa = 3.52 LAFMEE767 pKa = 4.02 QWTYY771 pKa = 11.14 RR772 pKa = 11.84 DD773 pKa = 3.22 YY774 pKa = 11.41 GYY776 pKa = 9.13 TEE778 pKa = 4.29 EE779 pKa = 4.81 EE780 pKa = 3.97 VGEE783 pKa = 4.84 LIDD786 pKa = 3.82 EE787 pKa = 5.62 PIYY790 pKa = 9.68 STPGLGVLEE799 pKa = 5.16 DD800 pKa = 3.75 CTKK803 pKa = 10.83 LVDD806 pKa = 4.09 GEE808 pKa = 4.64 CVSEE812 pKa = 5.13 GNYY815 pKa = 10.29 SWGHH819 pKa = 5.93 DD820 pKa = 3.34 EE821 pKa = 4.44 YY822 pKa = 11.27 VYY824 pKa = 11.52 SMEE827 pKa = 6.48 DD828 pKa = 3.09 GDD830 pKa = 3.8 TPYY833 pKa = 11.39 AVLHH837 pKa = 6.36 IEE839 pKa = 4.25 NPVSYY844 pKa = 9.78 MKK846 pKa = 10.41 IEE848 pKa = 4.28 AFHH851 pKa = 6.9 ANADD855 pKa = 3.86 GTKK858 pKa = 9.77 GDD860 pKa = 4.54 PVSPDD865 pKa = 3.14 YY866 pKa = 11.38 NVVYY870 pKa = 10.57 EE871 pKa = 4.29 SDD873 pKa = 3.87 GEE875 pKa = 4.45 GAVPGIQVYY884 pKa = 9.22 QWDD887 pKa = 3.88 GTYY890 pKa = 10.76 VKK892 pKa = 10.58 SADD895 pKa = 3.34 SEE897 pKa = 4.61 QYY899 pKa = 11.06 LKK901 pKa = 11.06 ALDD904 pKa = 3.79 GDD906 pKa = 4.31 YY907 pKa = 10.83 VIEE910 pKa = 4.18 VTAVKK915 pKa = 10.67 GLGSIDD921 pKa = 3.9 NPDD924 pKa = 3.59 HH925 pKa = 7.3 VEE927 pKa = 3.88 VWTSPAFTIDD937 pKa = 3.33 RR938 pKa = 11.84 DD939 pKa = 4.11 GVPGEE944 pKa = 4.32 GPGDD948 pKa = 3.64 GSIDD952 pKa = 3.54 EE953 pKa = 4.53 EE954 pKa = 4.24 ADD956 pKa = 3.4 KK957 pKa = 11.61 GFFLYY962 pKa = 11.01 NDD964 pKa = 3.92 FEE966 pKa = 4.73 SAADD970 pKa = 3.51 HH971 pKa = 5.94 YY972 pKa = 11.47 FMYY975 pKa = 10.54 GRR977 pKa = 11.84 DD978 pKa = 3.52 GDD980 pKa = 4.19 EE981 pKa = 4.29 VFVGDD986 pKa = 4.12 WDD988 pKa = 4.13 ADD990 pKa = 3.84 GVDD993 pKa = 3.94 TIGVRR998 pKa = 11.84 RR999 pKa = 11.84 GHH1001 pKa = 5.62 TFYY1004 pKa = 11.25 VQNQLIGGNAEE1015 pKa = 3.88 VEE1017 pKa = 4.81 FKK1019 pKa = 10.73 FGRR1022 pKa = 11.84 DD1023 pKa = 2.91 GDD1025 pKa = 4.34 EE1026 pKa = 4.09 ILVGDD1031 pKa = 4.83 WNADD1035 pKa = 3.32 GYY1037 pKa = 10.48 DD1038 pKa = 3.39 TFAVRR1043 pKa = 11.84 RR1044 pKa = 11.84 GVTIYY1049 pKa = 10.7 AQNEE1053 pKa = 4.58 LIGGNAEE1060 pKa = 3.95 VEE1062 pKa = 4.56 FKK1064 pKa = 10.65 YY1065 pKa = 11.07 GRR1067 pKa = 11.84 VGDD1070 pKa = 4.54 DD1071 pKa = 4.11 LFAGDD1076 pKa = 4.53 WDD1078 pKa = 3.85 GDD1080 pKa = 4.09 GYY1082 pKa = 10.67 DD1083 pKa = 3.35 TFAVRR1088 pKa = 11.84 RR1089 pKa = 11.84 GNSFYY1094 pKa = 11.13 VQNSLVGGNAEE1105 pKa = 4.05 DD1106 pKa = 3.9 TFHH1109 pKa = 7.28 FGHH1112 pKa = 6.72 EE1113 pKa = 4.34 SDD1115 pKa = 3.81 TVLVGDD1121 pKa = 4.15 WDD1123 pKa = 4.29 GDD1125 pKa = 4.01 LKK1127 pKa = 11.08 DD1128 pKa = 3.74 TVALRR1133 pKa = 11.84 RR1134 pKa = 11.84 GDD1136 pKa = 3.42 TFYY1139 pKa = 11.56 VSNSLSSGSADD1150 pKa = 3.03 RR1151 pKa = 11.84 SFVLGRR1157 pKa = 11.84 DD1158 pKa = 3.18 SDD1160 pKa = 4.07 EE1161 pKa = 4.35 VLVGDD1166 pKa = 4.31 WNGDD1170 pKa = 3.76 GIDD1173 pKa = 3.68 SVGLRR1178 pKa = 11.84 RR1179 pKa = 4.23
Molecular weight: 123.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.69
IPC2_protein 3.795
IPC_protein 3.834
Toseland 3.617
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.745
Rodwell 3.656
Grimsley 3.516
Solomon 3.821
Lehninger 3.783
Nozaki 3.923
DTASelect 4.177
Thurlkill 3.656
EMBOSS 3.757
Sillero 3.948
Patrickios 1.316
IPC_peptide 3.821
IPC2_peptide 3.935
IPC2.peptide.svr19 3.847
Protein with the highest isoelectric point:
>tr|A0A3Q9G536|A0A3Q9G536_9ACTO Riboflavin synthase OS=Flaviflexus sp. H23T48 OX=2496867 GN=EJ997_09760 PE=4 SV=1
MM1 pKa = 7.41 VKK3 pKa = 9.03 RR4 pKa = 11.84 TFQPNNRR11 pKa = 11.84 KK12 pKa = 8.78 RR13 pKa = 11.84 AKK15 pKa = 9.18 VHH17 pKa = 5.49 GFRR20 pKa = 11.84 KK21 pKa = 9.95 RR22 pKa = 11.84 MSTRR26 pKa = 11.84 AGRR29 pKa = 11.84 AILSGRR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.9 GRR40 pKa = 11.84 AKK42 pKa = 10.68 LSAA45 pKa = 3.92
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.705
Grimsley 13.042
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.427
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2334
0
2334
747752
32
2932
320.4
34.72
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.771 ± 0.054
0.647 ± 0.015
6.36 ± 0.047
6.808 ± 0.05
3.135 ± 0.031
8.593 ± 0.05
1.964 ± 0.022
5.619 ± 0.036
3.143 ± 0.038
9.424 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.241 ± 0.024
2.853 ± 0.026
4.989 ± 0.034
2.976 ± 0.027
6.03 ± 0.053
6.282 ± 0.039
6.26 ± 0.048
8.132 ± 0.047
1.434 ± 0.02
2.34 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here