Amino acid dipepetide frequency for Flaviflexus sp. H23T48

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	12.027AlaAla: 12.027 ± 0.165	0.849AlaCys: 0.849 ± 0.037	6.854AlaAsp: 6.854 ± 0.13	7.428AlaGlu: 7.428 ± 0.125	3.26AlaPhe: 3.26 ± 0.083	9.72AlaGly: 9.72 ± 0.147	1.971AlaHis: 1.971 ± 0.057	5.97AlaIle: 5.97 ± 0.098	3.497AlaLys: 3.497 ± 0.088	10.831AlaLeu: 10.831 ± 0.149	2.717AlaMet: 2.717 ± 0.063	2.867AlaAsn: 2.867 ± 0.059	4.606AlaPro: 4.606 ± 0.082	3.478AlaGln: 3.478 ± 0.08	6.41AlaArg: 6.41 ± 0.103	6.269AlaSer: 6.269 ± 0.105	6.506AlaThr: 6.506 ± 0.112	8.53AlaVal: 8.53 ± 0.115	1.562AlaTrp: 1.562 ± 0.056	2.355AlaTyr: 2.355 ± 0.054	0.0AlaXaa: 0.0 ± 0.0
Cys	0.596CysAla: 0.596 ± 0.028	0.08CysCys: 0.08 ± 0.013	0.358CysAsp: 0.358 ± 0.023	0.404CysGlu: 0.404 ± 0.025	0.198CysPhe: 0.198 ± 0.016	0.772CysGly: 0.772 ± 0.037	0.136CysHis: 0.136 ± 0.016	0.332CysIle: 0.332 ± 0.02	0.123CysLys: 0.123 ± 0.013	0.607CysLeu: 0.607 ± 0.03	0.12CysMet: 0.12 ± 0.012	0.154CysAsn: 0.154 ± 0.015	0.348CysPro: 0.348 ± 0.026	0.206CysGln: 0.206 ± 0.017	0.416CysArg: 0.416 ± 0.024	0.459CysSer: 0.459 ± 0.022	0.432CysThr: 0.432 ± 0.029	0.5CysVal: 0.5 ± 0.031	0.095CysTrp: 0.095 ± 0.011	0.134CysTyr: 0.134 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	6.014AspAla: 6.014 ± 0.098	0.349AspCys: 0.349 ± 0.019	4.019AspAsp: 4.019 ± 0.099	5.074AspGlu: 5.074 ± 0.093	1.93AspPhe: 1.93 ± 0.051	5.747AspGly: 5.747 ± 0.114	1.363AspHis: 1.363 ± 0.041	3.461AspIle: 3.461 ± 0.073	1.68AspLys: 1.68 ± 0.058	6.171AspLeu: 6.171 ± 0.107	1.367AspMet: 1.367 ± 0.045	1.593AspAsn: 1.593 ± 0.05	3.888AspPro: 3.888 ± 0.077	1.855AspGln: 1.855 ± 0.053	4.015AspArg: 4.015 ± 0.079	3.797AspSer: 3.797 ± 0.087	3.476AspThr: 3.476 ± 0.08	5.157AspVal: 5.157 ± 0.103	1.023AspTrp: 1.023 ± 0.046	1.624AspTyr: 1.624 ± 0.05	0.0AspXaa: 0.0 ± 0.0
Glu	7.5GluAla: 7.5 ± 0.121	0.326GluCys: 0.326 ± 0.022	4.162GluAsp: 4.162 ± 0.086	5.307GluGlu: 5.307 ± 0.106	2.019GluPhe: 2.019 ± 0.053	4.933GluGly: 4.933 ± 0.091	1.418GluHis: 1.418 ± 0.049	3.971GluIle: 3.971 ± 0.083	2.52GluLys: 2.52 ± 0.072	6.818GluLeu: 6.818 ± 0.092	1.439GluMet: 1.439 ± 0.047	2.298GluAsn: 2.298 ± 0.063	3.227GluPro: 3.227 ± 0.077	2.518GluGln: 2.518 ± 0.071	4.385GluArg: 4.385 ± 0.089	3.834GluSer: 3.834 ± 0.079	3.904GluThr: 3.904 ± 0.074	4.955GluVal: 4.955 ± 0.097	0.941GluTrp: 0.941 ± 0.041	1.606GluTyr: 1.606 ± 0.053	0.0GluXaa: 0.0 ± 0.0
Phe	3.333PheAla: 3.333 ± 0.073	0.221PheCys: 0.221 ± 0.018	2.221PheAsp: 2.221 ± 0.059	1.915PheGlu: 1.915 ± 0.055	1.176PhePhe: 1.176 ± 0.045	3.063PheGly: 3.063 ± 0.07	0.634PheHis: 0.634 ± 0.026	1.743PheIle: 1.743 ± 0.056	0.756PheLys: 0.756 ± 0.03	2.93PheLeu: 2.93 ± 0.075	0.627PheMet: 0.627 ± 0.029	0.911PheAsn: 0.911 ± 0.039	1.511PhePro: 1.511 ± 0.046	0.8PheGln: 0.8 ± 0.032	1.527PheArg: 1.527 ± 0.044	2.076PheSer: 2.076 ± 0.054	2.203PheThr: 2.203 ± 0.06	2.525PheVal: 2.525 ± 0.068	0.425PheTrp: 0.425 ± 0.024	0.753PheTyr: 0.753 ± 0.039	0.0PheXaa: 0.0 ± 0.0
Gly	8.39GlyAla: 8.39 ± 0.121	0.546GlyCys: 0.546 ± 0.027	5.205GlyAsp: 5.205 ± 0.106	5.472GlyGlu: 5.472 ± 0.097	2.953GlyPhe: 2.953 ± 0.073	6.923GlyGly: 6.923 ± 0.125	1.761GlyHis: 1.761 ± 0.05	5.193GlyIle: 5.193 ± 0.093	3.206GlyLys: 3.206 ± 0.073	7.997GlyLeu: 7.997 ± 0.122	2.295GlyMet: 2.295 ± 0.067	2.562GlyAsn: 2.562 ± 0.075	3.619GlyPro: 3.619 ± 0.076	2.834GlyGln: 2.834 ± 0.069	5.04GlyArg: 5.04 ± 0.084	5.422GlySer: 5.422 ± 0.104	5.899GlyThr: 5.899 ± 0.106	6.717GlyVal: 6.717 ± 0.102	1.438GlyTrp: 1.438 ± 0.047	2.454GlyTyr: 2.454 ± 0.061	0.0GlyXaa: 0.0 ± 0.0
His	1.912HisAla: 1.912 ± 0.047	0.15HisCys: 0.15 ± 0.013	1.256HisAsp: 1.256 ± 0.044	1.34HisGlu: 1.34 ± 0.038	0.543HisPhe: 0.543 ± 0.025	1.842HisGly: 1.842 ± 0.055	0.512HisHis: 0.512 ± 0.028	0.967HisIle: 0.967 ± 0.037	0.42HisLys: 0.42 ± 0.024	1.872HisLeu: 1.872 ± 0.059	0.419HisMet: 0.419 ± 0.024	0.519HisAsn: 0.519 ± 0.024	1.281HisPro: 1.281 ± 0.044	0.54HisGln: 0.54 ± 0.026	1.408HisArg: 1.408 ± 0.044	1.177HisSer: 1.177 ± 0.039	1.087HisThr: 1.087 ± 0.034	1.611HisVal: 1.611 ± 0.046	0.281HisTrp: 0.281 ± 0.022	0.506HisTyr: 0.506 ± 0.027	0.0HisXaa: 0.0 ± 0.0
Ile	6.711IleAla: 6.711 ± 0.119	0.384IleCys: 0.384 ± 0.02	4.13IleAsp: 4.13 ± 0.065	3.825IleGlu: 3.825 ± 0.08	1.709IlePhe: 1.709 ± 0.056	5.022IleGly: 5.022 ± 0.092	1.04IleHis: 1.04 ± 0.038	3.355IleIle: 3.355 ± 0.094	1.44IleLys: 1.44 ± 0.05	5.069IleLeu: 5.069 ± 0.097	1.074IleMet: 1.074 ± 0.046	1.559IleAsn: 1.559 ± 0.055	2.992IlePro: 2.992 ± 0.065	1.296IleGln: 1.296 ± 0.041	2.873IleArg: 2.873 ± 0.058	3.392IleSer: 3.392 ± 0.07	3.4IleThr: 3.4 ± 0.065	5.174IleVal: 5.174 ± 0.1	0.574IleTrp: 0.574 ± 0.031	1.174IleTyr: 1.174 ± 0.039	0.0IleXaa: 0.0 ± 0.0
Lys	3.441LysAla: 3.441 ± 0.081	0.134LysCys: 0.134 ± 0.015	1.99LysAsp: 1.99 ± 0.055	2.109LysGlu: 2.109 ± 0.058	0.721LysPhe: 0.721 ± 0.035	2.175LysGly: 2.175 ± 0.059	0.631LysHis: 0.631 ± 0.027	1.779LysIle: 1.779 ± 0.048	1.621LysLys: 1.621 ± 0.06	2.882LysLeu: 2.882 ± 0.064	0.733LysMet: 0.733 ± 0.03	1.155LysAsn: 1.155 ± 0.038	1.702LysPro: 1.702 ± 0.054	0.983LysGln: 0.983 ± 0.037	2.085LysArg: 2.085 ± 0.06	1.813LysSer: 1.813 ± 0.051	1.935LysThr: 1.935 ± 0.056	2.478LysVal: 2.478 ± 0.061	0.328LysTrp: 0.328 ± 0.022	0.734LysTyr: 0.734 ± 0.034	0.0LysXaa: 0.0 ± 0.0
Leu	11.412LeuAla: 11.412 ± 0.16	0.615LeuCys: 0.615 ± 0.027	6.284LeuAsp: 6.284 ± 0.084	6.015LeuGlu: 6.015 ± 0.1	2.802LeuPhe: 2.802 ± 0.07	8.227LeuGly: 8.227 ± 0.129	1.685LeuHis: 1.685 ± 0.055	5.186LeuIle: 5.186 ± 0.095	2.735LeuLys: 2.735 ± 0.061	8.9LeuLeu: 8.9 ± 0.169	1.924LeuMet: 1.924 ± 0.054	2.505LeuAsn: 2.505 ± 0.058	4.865LeuPro: 4.865 ± 0.089	2.24LeuGln: 2.24 ± 0.055	5.467LeuArg: 5.467 ± 0.089	6.175LeuSer: 6.175 ± 0.096	6.29LeuThr: 6.29 ± 0.096	8.071LeuVal: 8.071 ± 0.118	1.127LeuTrp: 1.127 ± 0.04	1.713LeuTyr: 1.713 ± 0.053	0.0LeuXaa: 0.0 ± 0.0
Met	2.474MetAla: 2.474 ± 0.061	0.139MetCys: 0.139 ± 0.014	1.173MetAsp: 1.173 ± 0.04	1.178MetGlu: 1.178 ± 0.044	0.679MetPhe: 0.679 ± 0.033	1.793MetGly: 1.793 ± 0.045	0.362MetHis: 0.362 ± 0.024	1.214MetIle: 1.214 ± 0.043	0.92MetLys: 0.92 ± 0.036	1.979MetLeu: 1.979 ± 0.051	0.457MetMet: 0.457 ± 0.028	0.765MetAsn: 0.765 ± 0.031	1.237MetPro: 1.237 ± 0.041	0.538MetGln: 0.538 ± 0.025	1.333MetArg: 1.333 ± 0.042	1.904MetSer: 1.904 ± 0.047	1.91MetThr: 1.91 ± 0.053	1.637MetVal: 1.637 ± 0.051	0.318MetTrp: 0.318 ± 0.024	0.399MetTyr: 0.399 ± 0.026	0.0MetXaa: 0.0 ± 0.0
Asn	2.898AsnAla: 2.898 ± 0.079	0.191AsnCys: 0.191 ± 0.016	1.717AsnAsp: 1.717 ± 0.06	1.838AsnGlu: 1.838 ± 0.054	0.8AsnPhe: 0.8 ± 0.035	2.481AsnGly: 2.481 ± 0.069	0.602AsnHis: 0.602 ± 0.027	1.495AsnIle: 1.495 ± 0.051	0.776AsnLys: 0.776 ± 0.04	2.491AsnLeu: 2.491 ± 0.061	0.667AsnMet: 0.667 ± 0.03	0.841AsnAsn: 0.841 ± 0.036	2.082AsnPro: 2.082 ± 0.054	0.944AsnGln: 0.944 ± 0.041	1.811AsnArg: 1.811 ± 0.047	1.72AsnSer: 1.72 ± 0.052	1.594AsnThr: 1.594 ± 0.06	2.306AsnVal: 2.306 ± 0.057	0.465AsnTrp: 0.465 ± 0.022	0.809AsnTyr: 0.809 ± 0.032	0.0AsnXaa: 0.0 ± 0.0
Pro	5.402ProAla: 5.402 ± 0.088	0.261ProCys: 0.261 ± 0.021	3.507ProAsp: 3.507 ± 0.062	4.092ProGlu: 4.092 ± 0.071	1.579ProPhe: 1.579 ± 0.048	4.721ProGly: 4.721 ± 0.09	1.052ProHis: 1.052 ± 0.044	2.564ProIle: 2.564 ± 0.058	1.522ProLys: 1.522 ± 0.049	4.079ProLeu: 4.079 ± 0.078	1.018ProMet: 1.018 ± 0.035	1.439ProAsn: 1.439 ± 0.049	1.756ProPro: 1.756 ± 0.057	1.411ProGln: 1.411 ± 0.048	2.552ProArg: 2.552 ± 0.065	3.301ProSer: 3.301 ± 0.066	3.386ProThr: 3.386 ± 0.09	4.306ProVal: 4.306 ± 0.088	0.734ProTrp: 0.734 ± 0.036	1.205ProTyr: 1.205 ± 0.043	0.0ProXaa: 0.0 ± 0.0
Gln	3.504GlnAla: 3.504 ± 0.065	0.185GlnCys: 0.185 ± 0.017	1.475GlnAsp: 1.475 ± 0.049	1.868GlnGlu: 1.868 ± 0.051	0.873GlnPhe: 0.873 ± 0.036	2.24GlnGly: 2.24 ± 0.055	0.622GlnHis: 0.622 ± 0.026	1.827GlnIle: 1.827 ± 0.05	0.966GlnLys: 0.966 ± 0.038	2.879GlnLeu: 2.879 ± 0.069	0.745GlnMet: 0.745 ± 0.031	0.841GlnAsn: 0.841 ± 0.035	1.462GlnPro: 1.462 ± 0.053	1.17GlnGln: 1.17 ± 0.051	1.962GlnArg: 1.962 ± 0.065	1.784GlnSer: 1.784 ± 0.049	1.63GlnThr: 1.63 ± 0.049	2.553GlnVal: 2.553 ± 0.052	0.448GlnTrp: 0.448 ± 0.028	0.722GlnTyr: 0.722 ± 0.037	0.0GlnXaa: 0.0 ± 0.0
Arg	6.1ArgAla: 6.1 ± 0.105	0.389ArgCys: 0.389 ± 0.024	3.501ArgAsp: 3.501 ± 0.069	4.345ArgGlu: 4.345 ± 0.09	1.906ArgPhe: 1.906 ± 0.054	4.574ArgGly: 4.574 ± 0.081	1.333ArgHis: 1.333 ± 0.042	3.464ArgIle: 3.464 ± 0.077	2.271ArgLys: 2.271 ± 0.055	5.573ArgLeu: 5.573 ± 0.102	1.533ArgMet: 1.533 ± 0.046	1.732ArgAsn: 1.732 ± 0.048	2.8ArgPro: 2.8 ± 0.066	2.014ArgGln: 2.014 ± 0.056	4.503ArgArg: 4.503 ± 0.098	3.837ArgSer: 3.837 ± 0.071	3.587ArgThr: 3.587 ± 0.073	4.411ArgVal: 4.411 ± 0.092	0.947ArgTrp: 0.947 ± 0.033	1.484ArgTyr: 1.484 ± 0.042	0.0ArgXaa: 0.0 ± 0.0
Ser	6.647SerAla: 6.647 ± 0.111	0.421SerCys: 0.421 ± 0.03	3.652SerAsp: 3.652 ± 0.071	3.93SerGlu: 3.93 ± 0.078	2.2SerPhe: 2.2 ± 0.059	5.87SerGly: 5.87 ± 0.098	1.277SerHis: 1.277 ± 0.04	3.556SerIle: 3.556 ± 0.077	1.888SerLys: 1.888 ± 0.047	5.793SerLeu: 5.793 ± 0.099	1.519SerMet: 1.519 ± 0.045	1.63SerAsn: 1.63 ± 0.046	3.095SerPro: 3.095 ± 0.078	1.962SerGln: 1.962 ± 0.049	3.916SerArg: 3.916 ± 0.07	4.163SerSer: 4.163 ± 0.096	3.928SerThr: 3.928 ± 0.091	4.765SerVal: 4.765 ± 0.07	1.023SerTrp: 1.023 ± 0.039	1.582SerTyr: 1.582 ± 0.048	0.0SerXaa: 0.0 ± 0.0
Thr	6.569ThrAla: 6.569 ± 0.101	0.385ThrCys: 0.385 ± 0.027	3.993ThrAsp: 3.993 ± 0.073	4.084ThrGlu: 4.084 ± 0.083	2.049ThrPhe: 2.049 ± 0.059	5.943ThrGly: 5.943 ± 0.084	1.099ThrHis: 1.099 ± 0.038	3.769ThrIle: 3.769 ± 0.083	1.781ThrLys: 1.781 ± 0.053	5.5ThrLeu: 5.5 ± 0.089	1.301ThrMet: 1.301 ± 0.04	1.763ThrAsn: 1.763 ± 0.057	3.401ThrPro: 3.401 ± 0.064	1.672ThrGln: 1.672 ± 0.049	3.171ThrArg: 3.171 ± 0.065	3.912ThrSer: 3.912 ± 0.086	3.865ThrThr: 3.865 ± 0.08	5.784ThrVal: 5.784 ± 0.114	0.996ThrTrp: 0.996 ± 0.046	1.565ThrTyr: 1.565 ± 0.047	0.0ThrXaa: 0.0 ± 0.0
Val	9.178ValAla: 9.178 ± 0.117	0.566ValCys: 0.566 ± 0.027	5.561ValAsp: 5.561 ± 0.12	5.34ValGlu: 5.34 ± 0.109	2.615ValPhe: 2.615 ± 0.062	6.528ValGly: 6.528 ± 0.104	1.396ValHis: 1.396 ± 0.05	4.567ValIle: 4.567 ± 0.092	2.319ValLys: 2.319 ± 0.054	8.029ValLeu: 8.029 ± 0.121	1.689ValMet: 1.689 ± 0.052	2.261ValAsn: 2.261 ± 0.061	4.166ValPro: 4.166 ± 0.085	2.01ValGln: 2.01 ± 0.053	4.788ValArg: 4.788 ± 0.085	5.28ValSer: 5.28 ± 0.089	5.455ValThr: 5.455 ± 0.114	6.922ValVal: 6.922 ± 0.099	0.971ValTrp: 0.971 ± 0.036	1.677ValTyr: 1.677 ± 0.047	0.0ValXaa: 0.0 ± 0.0
Trp	1.34TrpAla: 1.34 ± 0.045	0.095TrpCys: 0.095 ± 0.012	0.966TrpAsp: 0.966 ± 0.049	0.871TrpGlu: 0.871 ± 0.034	0.499TrpPhe: 0.499 ± 0.028	1.008TrpGly: 1.008 ± 0.043	0.301TrpHis: 0.301 ± 0.022	0.806TrpIle: 0.806 ± 0.034	0.485TrpLys: 0.485 ± 0.024	1.454TrpLeu: 1.454 ± 0.046	0.365TrpMet: 0.365 ± 0.023	0.531TrpAsn: 0.531 ± 0.031	0.635TrpPro: 0.635 ± 0.029	0.576TrpGln: 0.576 ± 0.029	0.921TrpArg: 0.921 ± 0.037	0.925TrpSer: 0.925 ± 0.035	0.849TrpThr: 0.849 ± 0.039	1.079TrpVal: 1.079 ± 0.039	0.322TrpTrp: 0.322 ± 0.021	0.312TrpTyr: 0.312 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.261TyrAla: 2.261 ± 0.054	0.185TyrCys: 0.185 ± 0.016	1.575TyrAsp: 1.575 ± 0.054	1.645TyrGlu: 1.645 ± 0.052	0.837TyrPhe: 0.837 ± 0.036	2.347TyrGly: 2.347 ± 0.071	0.445TyrHis: 0.445 ± 0.027	0.974TyrIle: 0.974 ± 0.032	0.504TyrLys: 0.504 ± 0.027	2.384TyrLeu: 2.384 ± 0.058	0.4TyrMet: 0.4 ± 0.023	0.602TyrAsn: 0.602 ± 0.029	1.213TyrPro: 1.213 ± 0.039	0.709TyrGln: 0.709 ± 0.039	1.72TyrArg: 1.72 ± 0.057	1.579TyrSer: 1.579 ± 0.057	1.268TyrThr: 1.268 ± 0.051	1.836TyrVal: 1.836 ± 0.054	0.322TyrTrp: 0.322 ± 0.02	0.598TyrTyr: 0.598 ± 0.025	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2334 proteins (747752 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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