Mycobacterium phage Estave1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 112 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A088F7M6|A0A088F7M6_9CAUD Uncharacterized protein OS=Mycobacterium phage Estave1 OX=1536603 GN=PBI_ESTAVE1_107 PE=4 SV=1
MM1 pKa = 7.42RR2 pKa = 11.84RR3 pKa = 11.84WTGADD8 pKa = 2.67WMNPDD13 pKa = 4.6AQLFWEE19 pKa = 5.16NYY21 pKa = 8.57HH22 pKa = 6.88PDD24 pKa = 3.0WDD26 pKa = 4.74DD27 pKa = 3.81YY28 pKa = 10.47QGSWEE33 pKa = 4.06SLRR36 pKa = 11.84YY37 pKa = 8.02WVEE40 pKa = 4.1VEE42 pKa = 4.47CAAALHH48 pKa = 6.01GMDD51 pKa = 3.57VAMGRR56 pKa = 11.84PTPPPVARR64 pKa = 11.84VVTVDD69 pKa = 3.42EE70 pKa = 4.8DD71 pKa = 4.04LGDD74 pKa = 3.62SRR76 pKa = 11.84SITRR80 pKa = 11.84TFGGGCATCDD90 pKa = 3.06GGGCGDD96 pKa = 4.53CGEE99 pKa = 4.38EE100 pKa = 3.95AA101 pKa = 4.27

Molecular weight: 11.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A088F6C9|A0A088F6C9_9CAUD Uncharacterized protein OS=Mycobacterium phage Estave1 OX=1536603 GN=PBI_ESTAVE1_88 PE=4 SV=1
MM1 pKa = 7.49SGRR4 pKa = 11.84FRR6 pKa = 11.84TLEE9 pKa = 3.77QVISEE14 pKa = 4.28WGFDD18 pKa = 3.34TSDD21 pKa = 2.99EE22 pKa = 4.04EE23 pKa = 3.84RR24 pKa = 11.84RR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84VRR29 pKa = 11.84WLADD33 pKa = 4.29LIRR36 pKa = 11.84AGKK39 pKa = 9.92VPAHH43 pKa = 6.24KK44 pKa = 10.21FGRR47 pKa = 11.84SWYY50 pKa = 9.72MDD52 pKa = 3.49DD53 pKa = 3.69TDD55 pKa = 3.45IAAALGVLEE64 pKa = 5.5NGFQKK69 pKa = 10.53PVVEE73 pKa = 4.46RR74 pKa = 11.84PVEE77 pKa = 3.9PVRR80 pKa = 11.84RR81 pKa = 11.84GPSAASMRR89 pKa = 11.84RR90 pKa = 11.84RR91 pKa = 11.84LASS94 pKa = 3.18

Molecular weight: 10.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

112

0

112

19508

25

1211

174.2

19.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.734 ± 0.455

1.312 ± 0.156

6.536 ± 0.198

6.044 ± 0.33

2.891 ± 0.149

8.607 ± 0.538

2.368 ± 0.189

4.378 ± 0.177

3.265 ± 0.163

7.387 ± 0.215

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.189 ± 0.111

3.235 ± 0.152

5.962 ± 0.19

3.486 ± 0.194

7.105 ± 0.374

5.787 ± 0.256

6.679 ± 0.266

7.187 ± 0.237

2.322 ± 0.133

2.527 ± 0.119
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski