Amino acid dipepetide frequency for Mycobacterium phage Estave1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.277AlaAla: 15.277 ± 1.748
1.128AlaCys: 1.128 ± 0.279
7.228AlaAsp: 7.228 ± 0.539
7.331AlaGlu: 7.331 ± 0.762
2.358AlaPhe: 2.358 ± 0.307
10.15AlaGly: 10.15 ± 1.155
1.999AlaHis: 1.999 ± 0.328
4.101AlaIle: 4.101 ± 0.532
4.255AlaLys: 4.255 ± 0.353
8.51AlaLeu: 8.51 ± 0.721
2.409AlaMet: 2.409 ± 0.392
3.076AlaAsn: 3.076 ± 0.382
4.921AlaPro: 4.921 ± 0.539
3.332AlaGln: 3.332 ± 0.604
7.228AlaArg: 7.228 ± 0.703
5.536AlaSer: 5.536 ± 0.546
6.152AlaThr: 6.152 ± 0.826
7.382AlaVal: 7.382 ± 0.588
2.512AlaTrp: 2.512 ± 0.409
2.461AlaTyr: 2.461 ± 0.397
0.0AlaXaa: 0.0 ± 0.0
Cys
1.025CysAla: 1.025 ± 0.272
0.205CysCys: 0.205 ± 0.115
1.077CysAsp: 1.077 ± 0.289
1.128CysGlu: 1.128 ± 0.266
0.461CysPhe: 0.461 ± 0.157
1.794CysGly: 1.794 ± 0.453
0.205CysHis: 0.205 ± 0.097
0.256CysIle: 0.256 ± 0.121
0.615CysLys: 0.615 ± 0.177
0.564CysLeu: 0.564 ± 0.184
0.103CysMet: 0.103 ± 0.063
0.41CysAsn: 0.41 ± 0.144
1.23CysPro: 1.23 ± 0.26
0.359CysGln: 0.359 ± 0.15
0.769CysArg: 0.769 ± 0.252
1.077CysSer: 1.077 ± 0.265
0.666CysThr: 0.666 ± 0.216
0.666CysVal: 0.666 ± 0.165
0.205CysTrp: 0.205 ± 0.102
0.308CysTyr: 0.308 ± 0.11
0.0CysXaa: 0.0 ± 0.0
Asp
5.895AspAla: 5.895 ± 0.626
0.82AspCys: 0.82 ± 0.233
4.46AspAsp: 4.46 ± 0.609
4.306AspGlu: 4.306 ± 0.53
1.538AspPhe: 1.538 ± 0.285
6.305AspGly: 6.305 ± 0.443
1.64AspHis: 1.64 ± 0.241
2.563AspIle: 2.563 ± 0.419
1.743AspLys: 1.743 ± 0.268
5.588AspLeu: 5.588 ± 0.565
1.435AspMet: 1.435 ± 0.304
1.589AspAsn: 1.589 ± 0.309
3.691AspPro: 3.691 ± 0.53
2.666AspGln: 2.666 ± 0.437
4.973AspArg: 4.973 ± 0.598
3.537AspSer: 3.537 ± 0.514
4.665AspThr: 4.665 ± 0.465
4.409AspVal: 4.409 ± 0.407
1.794AspTrp: 1.794 ± 0.32
1.743AspTyr: 1.743 ± 0.319
0.0AspXaa: 0.0 ± 0.0
Glu
5.844GluAla: 5.844 ± 0.655
1.179GluCys: 1.179 ± 0.327
3.076GluAsp: 3.076 ± 0.471
2.973GluGlu: 2.973 ± 0.531
2.563GluPhe: 2.563 ± 0.409
2.461GluGly: 2.461 ± 0.342
1.845GluHis: 1.845 ± 0.361
1.999GluIle: 1.999 ± 0.365
1.589GluLys: 1.589 ± 0.295
5.947GluLeu: 5.947 ± 0.664
1.64GluMet: 1.64 ± 0.295
2.051GluAsn: 2.051 ± 0.288
3.435GluPro: 3.435 ± 0.487
3.178GluGln: 3.178 ± 0.42
5.485GluArg: 5.485 ± 0.701
2.871GluSer: 2.871 ± 0.442
4.614GluThr: 4.614 ± 0.58
4.101GluVal: 4.101 ± 0.402
1.845GluTrp: 1.845 ± 0.306
1.743GluTyr: 1.743 ± 0.299
0.0GluXaa: 0.0 ± 0.0
Phe
3.281PheAla: 3.281 ± 0.395
0.308PheCys: 0.308 ± 0.139
2.614PheAsp: 2.614 ± 0.401
1.897PheGlu: 1.897 ± 0.285
0.718PhePhe: 0.718 ± 0.219
2.563PheGly: 2.563 ± 0.549
0.461PheHis: 0.461 ± 0.138
1.64PheIle: 1.64 ± 0.38
0.923PheLys: 0.923 ± 0.229
1.692PheLeu: 1.692 ± 0.277
0.82PheMet: 0.82 ± 0.204
1.025PheAsn: 1.025 ± 0.201
1.384PhePro: 1.384 ± 0.286
1.077PheGln: 1.077 ± 0.283
1.692PheArg: 1.692 ± 0.298
1.589PheSer: 1.589 ± 0.288
2.153PheThr: 2.153 ± 0.338
1.692PheVal: 1.692 ± 0.296
0.564PheTrp: 0.564 ± 0.141
0.82PheTyr: 0.82 ± 0.201
0.0PheXaa: 0.0 ± 0.0
Gly
8.561GlyAla: 8.561 ± 1.352
1.23GlyCys: 1.23 ± 0.304
5.793GlyAsp: 5.793 ± 0.444
4.819GlyGlu: 4.819 ± 0.55
2.358GlyPhe: 2.358 ± 0.333
10.407GlyGly: 10.407 ± 1.922
2.051GlyHis: 2.051 ± 0.295
3.127GlyIle: 3.127 ± 0.458
2.82GlyLys: 2.82 ± 0.383
5.024GlyLeu: 5.024 ± 0.593
1.999GlyMet: 1.999 ± 0.351
3.178GlyAsn: 3.178 ± 0.399
3.794GlyPro: 3.794 ± 0.637
2.871GlyGln: 2.871 ± 0.69
5.536GlyArg: 5.536 ± 0.515
5.69GlySer: 5.69 ± 0.806
5.844GlyThr: 5.844 ± 0.7
6.049GlyVal: 6.049 ± 0.501
2.563GlyTrp: 2.563 ± 0.338
2.358GlyTyr: 2.358 ± 0.345
0.0GlyXaa: 0.0 ± 0.0
His
2.153HisAla: 2.153 ± 0.314
0.513HisCys: 0.513 ± 0.17
1.538HisAsp: 1.538 ± 0.303
1.23HisGlu: 1.23 ± 0.224
0.41HisPhe: 0.41 ± 0.167
1.845HisGly: 1.845 ± 0.256
0.923HisHis: 0.923 ± 0.242
1.589HisIle: 1.589 ± 0.302
0.871HisLys: 0.871 ± 0.175
1.845HisLeu: 1.845 ± 0.305
0.461HisMet: 0.461 ± 0.136
0.82HisAsn: 0.82 ± 0.195
1.845HisPro: 1.845 ± 0.293
0.82HisGln: 0.82 ± 0.181
1.948HisArg: 1.948 ± 0.375
0.871HisSer: 0.871 ± 0.207
1.384HisThr: 1.384 ± 0.375
1.282HisVal: 1.282 ± 0.277
0.461HisTrp: 0.461 ± 0.146
0.871HisTyr: 0.871 ± 0.188
0.0HisXaa: 0.0 ± 0.0
Ile
5.588IleAla: 5.588 ± 0.476
0.666IleCys: 0.666 ± 0.242
3.537IleAsp: 3.537 ± 0.48
3.332IleGlu: 3.332 ± 0.339
0.871IlePhe: 0.871 ± 0.192
3.64IleGly: 3.64 ± 0.395
1.64IleHis: 1.64 ± 0.266
1.538IleIle: 1.538 ± 0.339
0.871IleLys: 0.871 ± 0.211
2.102IleLeu: 2.102 ± 0.44
0.461IleMet: 0.461 ± 0.149
2.051IleAsn: 2.051 ± 0.355
3.076IlePro: 3.076 ± 0.376
1.179IleGln: 1.179 ± 0.214
2.871IleArg: 2.871 ± 0.405
1.948IleSer: 1.948 ± 0.376
3.845IleThr: 3.845 ± 0.581
3.076IleVal: 3.076 ± 0.33
0.769IleTrp: 0.769 ± 0.187
0.718IleTyr: 0.718 ± 0.179
0.0IleXaa: 0.0 ± 0.0
Lys
3.742LysAla: 3.742 ± 0.409
0.461LysCys: 0.461 ± 0.138
1.743LysAsp: 1.743 ± 0.29
1.282LysGlu: 1.282 ± 0.23
1.23LysPhe: 1.23 ± 0.213
2.358LysGly: 2.358 ± 0.356
0.769LysHis: 0.769 ± 0.205
0.871LysIle: 0.871 ± 0.204
1.179LysLys: 1.179 ± 0.26
2.461LysLeu: 2.461 ± 0.53
0.513LysMet: 0.513 ± 0.157
1.077LysAsn: 1.077 ± 0.228
2.666LysPro: 2.666 ± 0.366
1.333LysGln: 1.333 ± 0.232
2.358LysArg: 2.358 ± 0.381
2.102LysSer: 2.102 ± 0.333
2.051LysThr: 2.051 ± 0.351
2.768LysVal: 2.768 ± 0.385
0.718LysTrp: 0.718 ± 0.235
0.974LysTyr: 0.974 ± 0.241
0.0LysXaa: 0.0 ± 0.0
Leu
9.586LeuAla: 9.586 ± 0.756
0.666LeuCys: 0.666 ± 0.204
4.819LeuAsp: 4.819 ± 0.635
4.05LeuGlu: 4.05 ± 0.397
2.461LeuPhe: 2.461 ± 0.286
5.331LeuGly: 5.331 ± 0.568
1.538LeuHis: 1.538 ± 0.343
2.973LeuIle: 2.973 ± 0.41
2.102LeuLys: 2.102 ± 0.377
5.844LeuLeu: 5.844 ± 0.711
1.435LeuMet: 1.435 ± 0.237
2.102LeuAsn: 2.102 ± 0.313
4.819LeuPro: 4.819 ± 0.691
2.358LeuGln: 2.358 ± 0.458
5.229LeuArg: 5.229 ± 0.719
4.562LeuSer: 4.562 ± 0.482
5.69LeuThr: 5.69 ± 0.532
4.87LeuVal: 4.87 ± 0.48
1.179LeuTrp: 1.179 ± 0.226
2.256LeuTyr: 2.256 ± 0.381
0.0LeuXaa: 0.0 ± 0.0
Met
1.999MetAla: 1.999 ± 0.331
0.308MetCys: 0.308 ± 0.128
1.077MetAsp: 1.077 ± 0.217
0.871MetGlu: 0.871 ± 0.213
0.82MetPhe: 0.82 ± 0.224
1.487MetGly: 1.487 ± 0.238
0.256MetHis: 0.256 ± 0.105
0.82MetIle: 0.82 ± 0.19
0.513MetLys: 0.513 ± 0.138
1.897MetLeu: 1.897 ± 0.287
0.564MetMet: 0.564 ± 0.194
0.871MetAsn: 0.871 ± 0.233
1.077MetPro: 1.077 ± 0.245
0.359MetGln: 0.359 ± 0.11
1.743MetArg: 1.743 ± 0.271
2.922MetSer: 2.922 ± 0.35
2.256MetThr: 2.256 ± 0.31
1.333MetVal: 1.333 ± 0.262
0.205MetTrp: 0.205 ± 0.088
0.513MetTyr: 0.513 ± 0.128
0.0MetXaa: 0.0 ± 0.0
Asn
2.973AsnAla: 2.973 ± 0.35
0.256AsnCys: 0.256 ± 0.102
1.538AsnAsp: 1.538 ± 0.234
2.051AsnGlu: 2.051 ± 0.36
0.513AsnPhe: 0.513 ± 0.163
4.152AsnGly: 4.152 ± 0.477
0.923AsnHis: 0.923 ± 0.254
1.538AsnIle: 1.538 ± 0.359
0.923AsnLys: 0.923 ± 0.199
2.307AsnLeu: 2.307 ± 0.352
0.564AsnMet: 0.564 ± 0.149
1.64AsnAsn: 1.64 ± 0.379
2.717AsnPro: 2.717 ± 0.361
0.82AsnGln: 0.82 ± 0.324
2.409AsnArg: 2.409 ± 0.414
1.435AsnSer: 1.435 ± 0.271
1.948AsnThr: 1.948 ± 0.363
2.102AsnVal: 2.102 ± 0.313
0.718AsnTrp: 0.718 ± 0.181
0.82AsnTyr: 0.82 ± 0.176
0.0AsnXaa: 0.0 ± 0.0
Pro
5.844ProAla: 5.844 ± 0.574
0.615ProCys: 0.615 ± 0.209
4.306ProAsp: 4.306 ± 0.469
4.152ProGlu: 4.152 ± 0.404
2.204ProPhe: 2.204 ± 0.307
5.998ProGly: 5.998 ± 0.642
1.692ProHis: 1.692 ± 0.274
1.948ProIle: 1.948 ± 0.271
1.794ProLys: 1.794 ± 0.338
4.101ProLeu: 4.101 ± 0.48
1.384ProMet: 1.384 ± 0.259
2.153ProAsn: 2.153 ± 0.335
3.896ProPro: 3.896 ± 0.58
2.051ProGln: 2.051 ± 0.377
3.025ProArg: 3.025 ± 0.501
3.076ProSer: 3.076 ± 0.362
3.794ProThr: 3.794 ± 0.425
5.075ProVal: 5.075 ± 0.48
1.179ProTrp: 1.179 ± 0.261
1.333ProTyr: 1.333 ± 0.227
0.0ProXaa: 0.0 ± 0.0
Gln
4.921GlnAla: 4.921 ± 0.429
0.513GlnCys: 0.513 ± 0.192
1.333GlnAsp: 1.333 ± 0.284
1.384GlnGlu: 1.384 ± 0.236
1.128GlnPhe: 1.128 ± 0.191
2.461GlnGly: 2.461 ± 0.528
0.666GlnHis: 0.666 ± 0.169
1.845GlnIle: 1.845 ± 0.292
1.282GlnLys: 1.282 ± 0.21
2.973GlnLeu: 2.973 ± 0.399
0.615GlnMet: 0.615 ± 0.162
0.923GlnAsn: 0.923 ± 0.255
2.922GlnPro: 2.922 ± 0.49
1.487GlnGln: 1.487 ± 0.319
2.461GlnArg: 2.461 ± 0.367
1.589GlnSer: 1.589 ± 0.247
1.538GlnThr: 1.538 ± 0.236
2.82GlnVal: 2.82 ± 0.394
0.82GlnTrp: 0.82 ± 0.245
1.179GlnTyr: 1.179 ± 0.316
0.0GlnXaa: 0.0 ± 0.0
Arg
7.177ArgAla: 7.177 ± 0.7
1.179ArgCys: 1.179 ± 0.26
4.46ArgAsp: 4.46 ± 0.534
4.87ArgGlu: 4.87 ± 0.645
1.999ArgPhe: 1.999 ± 0.34
4.101ArgGly: 4.101 ± 0.388
1.692ArgHis: 1.692 ± 0.301
4.562ArgIle: 4.562 ± 0.532
2.717ArgLys: 2.717 ± 0.369
5.639ArgLeu: 5.639 ± 0.612
2.153ArgMet: 2.153 ± 0.292
1.845ArgAsn: 1.845 ± 0.392
3.383ArgPro: 3.383 ± 0.393
1.999ArgGln: 1.999 ± 0.34
5.895ArgArg: 5.895 ± 0.799
3.999ArgSer: 3.999 ± 0.543
3.845ArgThr: 3.845 ± 0.56
5.28ArgVal: 5.28 ± 0.579
2.307ArgTrp: 2.307 ± 0.377
1.897ArgTyr: 1.897 ± 0.293
0.0ArgXaa: 0.0 ± 0.0
Ser
6.049SerAla: 6.049 ± 1.005
0.615SerCys: 0.615 ± 0.195
4.511SerAsp: 4.511 ± 0.508
3.127SerGlu: 3.127 ± 0.484
1.794SerPhe: 1.794 ± 0.306
6.305SerGly: 6.305 ± 0.926
1.179SerHis: 1.179 ± 0.25
2.871SerIle: 2.871 ± 0.346
2.153SerLys: 2.153 ± 0.336
4.204SerLeu: 4.204 ± 0.456
1.538SerMet: 1.538 ± 0.272
2.204SerAsn: 2.204 ± 0.295
3.435SerPro: 3.435 ± 0.334
1.845SerGln: 1.845 ± 0.222
3.537SerArg: 3.537 ± 0.388
3.486SerSer: 3.486 ± 0.622
2.973SerThr: 2.973 ± 0.441
3.691SerVal: 3.691 ± 0.426
1.179SerTrp: 1.179 ± 0.214
1.179SerTyr: 1.179 ± 0.202
0.0SerXaa: 0.0 ± 0.0
Thr
5.947ThrAla: 5.947 ± 0.602
0.718ThrCys: 0.718 ± 0.255
3.999ThrAsp: 3.999 ± 0.555
4.152ThrGlu: 4.152 ± 0.433
1.897ThrPhe: 1.897 ± 0.323
6.664ThrGly: 6.664 ± 0.65
1.64ThrHis: 1.64 ± 0.365
3.64ThrIle: 3.64 ± 0.484
2.512ThrLys: 2.512 ± 0.317
3.947ThrLeu: 3.947 ± 0.478
1.282ThrMet: 1.282 ± 0.239
1.897ThrAsn: 1.897 ± 0.267
4.562ThrPro: 4.562 ± 0.444
1.948ThrGln: 1.948 ± 0.266
4.614ThrArg: 4.614 ± 0.558
3.947ThrSer: 3.947 ± 0.587
4.562ThrThr: 4.562 ± 0.585
6.203ThrVal: 6.203 ± 0.576
1.077ThrTrp: 1.077 ± 0.282
1.589ThrTyr: 1.589 ± 0.294
0.0ThrXaa: 0.0 ± 0.0
Val
7.126ValAla: 7.126 ± 0.595
1.23ValCys: 1.23 ± 0.244
5.126ValAsp: 5.126 ± 0.487
4.562ValGlu: 4.562 ± 0.478
2.051ValPhe: 2.051 ± 0.343
4.819ValGly: 4.819 ± 0.464
1.384ValHis: 1.384 ± 0.249
3.537ValIle: 3.537 ± 0.427
2.358ValLys: 2.358 ± 0.321
5.485ValLeu: 5.485 ± 0.535
1.23ValMet: 1.23 ± 0.207
2.204ValAsn: 2.204 ± 0.301
4.255ValPro: 4.255 ± 0.407
2.82ValGln: 2.82 ± 0.338
5.024ValArg: 5.024 ± 0.591
4.973ValSer: 4.973 ± 0.645
5.383ValThr: 5.383 ± 0.537
5.434ValVal: 5.434 ± 0.614
1.538ValTrp: 1.538 ± 0.251
1.333ValTyr: 1.333 ± 0.229
0.0ValXaa: 0.0 ± 0.0
Trp
2.204TrpAla: 2.204 ± 0.354
0.359TrpCys: 0.359 ± 0.143
1.282TrpAsp: 1.282 ± 0.272
1.128TrpGlu: 1.128 ± 0.308
0.564TrpPhe: 0.564 ± 0.195
1.128TrpGly: 1.128 ± 0.246
0.615TrpHis: 0.615 ± 0.188
1.025TrpIle: 1.025 ± 0.216
0.82TrpLys: 0.82 ± 0.161
1.897TrpLeu: 1.897 ± 0.321
0.974TrpMet: 0.974 ± 0.201
0.359TrpAsn: 0.359 ± 0.125
1.128TrpPro: 1.128 ± 0.194
1.179TrpGln: 1.179 ± 0.277
2.102TrpArg: 2.102 ± 0.36
1.589TrpSer: 1.589 ± 0.376
1.589TrpThr: 1.589 ± 0.316
1.692TrpVal: 1.692 ± 0.363
0.871TrpTrp: 0.871 ± 0.198
0.718TrpTyr: 0.718 ± 0.179
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.153TyrAla: 2.153 ± 0.297
0.154TyrCys: 0.154 ± 0.097
1.845TyrAsp: 1.845 ± 0.288
1.794TyrGlu: 1.794 ± 0.29
0.974TyrPhe: 0.974 ± 0.226
2.102TyrGly: 2.102 ± 0.329
0.564TyrHis: 0.564 ± 0.144
1.333TyrIle: 1.333 ± 0.271
0.615TyrLys: 0.615 ± 0.171
1.845TyrLeu: 1.845 ± 0.263
0.256TyrMet: 0.256 ± 0.106
0.871TyrAsn: 0.871 ± 0.208
1.384TyrPro: 1.384 ± 0.254
1.179TyrGln: 1.179 ± 0.23
2.153TyrArg: 2.153 ± 0.396
1.077TyrSer: 1.077 ± 0.266
1.845TyrThr: 1.845 ± 0.312
1.948TyrVal: 1.948 ± 0.313
0.718TyrTrp: 0.718 ± 0.158
0.461TyrTyr: 0.461 ± 0.14
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 112 proteins (19508 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski