Human adenovirus D serotype 17 (HAdV-17) (Human adenovirus 17)
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q9WF16|Q9WF16_ADE17 Pre-hexon-linking protein IIIa OS=Human adenovirus D serotype 17 OX=46922 PE=2 SV=1
MM1 pKa = 7.31 HH2 pKa = 7.36 PVLRR6 pKa = 11.84 QMRR9 pKa = 11.84 PTPPATTATAAVAGAGASQPQPQTEE34 pKa = 3.87 MDD36 pKa = 3.68 LEE38 pKa = 4.42 EE39 pKa = 6.32 GEE41 pKa = 4.35 GLARR45 pKa = 11.84 LGAPSPEE52 pKa = 3.6 RR53 pKa = 11.84 HH54 pKa = 6.33 PRR56 pKa = 11.84 VQLQKK61 pKa = 10.54 DD62 pKa = 3.51 VRR64 pKa = 11.84 PAYY67 pKa = 10.69 VPAQNLFRR75 pKa = 11.84 DD76 pKa = 3.85 RR77 pKa = 11.84 SGEE80 pKa = 3.95 EE81 pKa = 3.87 PEE83 pKa = 4.09 EE84 pKa = 3.87 MRR86 pKa = 11.84 DD87 pKa = 3.31 CRR89 pKa = 11.84 FRR91 pKa = 11.84 AGRR94 pKa = 11.84 EE95 pKa = 3.76 LRR97 pKa = 11.84 EE98 pKa = 3.94 GLDD101 pKa = 3.31 RR102 pKa = 11.84 QRR104 pKa = 11.84 VLRR107 pKa = 11.84 DD108 pKa = 2.89 EE109 pKa = 5.21 DD110 pKa = 4.09 FEE112 pKa = 5.17 PNEE115 pKa = 3.9 QTGISPARR123 pKa = 11.84 AHH125 pKa = 5.74 VAAANLVTAYY135 pKa = 9.0 EE136 pKa = 4.04 QTVKK140 pKa = 10.49 QEE142 pKa = 4.4 RR143 pKa = 11.84 NFQKK147 pKa = 10.59 SFNNHH152 pKa = 3.71 VRR154 pKa = 11.84 TLIARR159 pKa = 11.84 EE160 pKa = 4.02 EE161 pKa = 4.21 VALGLMHH168 pKa = 7.41 LWDD171 pKa = 3.96 LAEE174 pKa = 5.92 AIVQNPDD181 pKa = 3.41 SKK183 pKa = 11.15 PLTAQLFLVVQHH195 pKa = 6.48 SRR197 pKa = 11.84 DD198 pKa = 3.36 NEE200 pKa = 3.99 AFRR203 pKa = 11.84 EE204 pKa = 4.03 ALLNIAEE211 pKa = 4.33 PEE213 pKa = 4.24 GRR215 pKa = 11.84 WLLEE219 pKa = 4.86 LINILQSIVVQEE231 pKa = 4.36 RR232 pKa = 11.84 SLSLAEE238 pKa = 4.47 KK239 pKa = 10.11 VAAINYY245 pKa = 9.0 SVLSLGKK252 pKa = 9.39 FYY254 pKa = 11.12 ARR256 pKa = 11.84 KK257 pKa = 9.39 IYY259 pKa = 7.63 KK260 pKa = 8.73 TPYY263 pKa = 8.86 VPIDD267 pKa = 3.65 KK268 pKa = 9.91 EE269 pKa = 4.28 VKK271 pKa = 9.4 IDD273 pKa = 3.6 SFYY276 pKa = 10.94 MRR278 pKa = 11.84 MALKK282 pKa = 10.67 VLTLSDD288 pKa = 3.71 DD289 pKa = 3.79 LGVYY293 pKa = 10.56 RR294 pKa = 11.84 NDD296 pKa = 4.41 RR297 pKa = 11.84 IHH299 pKa = 6.67 KK300 pKa = 9.47 AVSASRR306 pKa = 11.84 RR307 pKa = 11.84 RR308 pKa = 11.84 EE309 pKa = 3.75 LSDD312 pKa = 3.68 RR313 pKa = 11.84 EE314 pKa = 4.15 LMLSLRR320 pKa = 11.84 RR321 pKa = 11.84 ALVGGAAGGEE331 pKa = 3.78 EE332 pKa = 5.21 SYY334 pKa = 11.63 FDD336 pKa = 4.05 MGADD340 pKa = 3.61 LHH342 pKa = 5.83 WQPSRR347 pKa = 11.84 RR348 pKa = 11.84 ALEE351 pKa = 3.94 AAYY354 pKa = 10.02 GPEE357 pKa = 4.18 DD358 pKa = 3.77 LDD360 pKa = 3.74 EE361 pKa = 4.65 EE362 pKa = 4.49 EE363 pKa = 5.14 EE364 pKa = 4.29 EE365 pKa = 5.12 EE366 pKa = 4.28 EE367 pKa = 4.16 DD368 pKa = 4.86 APAAGYY374 pKa = 10.02
Molecular weight: 42.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.213
IPC2_protein 5.219
IPC_protein 5.169
Toseland 5.143
ProMoST 5.296
Dawson 5.156
Bjellqvist 5.283
Wikipedia 5.003
Rodwell 5.092
Grimsley 5.08
Solomon 5.156
Lehninger 5.118
Nozaki 5.283
DTASelect 5.397
Thurlkill 5.143
EMBOSS 5.067
Sillero 5.359
Patrickios 4.368
IPC_peptide 5.169
IPC2_peptide 5.372
IPC2.peptide.svr19 5.316
Protein with the highest isoelectric point:
>tr|Q9WF18|Q9WF18_ADE17 Isoform of Q9WF19 Pre-protein VI OS=Human adenovirus D serotype 17 OX=46922 GN=L3 PE=2 SV=1
MM1 pKa = 7.8 EE2 pKa = 6.49 DD3 pKa = 3.25 INFASLAPRR12 pKa = 11.84 HH13 pKa = 5.03 GTRR16 pKa = 11.84 PFMGTWNEE24 pKa = 3.89 IGTSQLNGGAFNWSSVWSGLKK45 pKa = 10.58 NFGSTLRR52 pKa = 11.84 TYY54 pKa = 11.16 GNKK57 pKa = 9.37 AWNSSTGQLLRR68 pKa = 11.84 EE69 pKa = 4.1 KK70 pKa = 10.86 LKK72 pKa = 10.96 DD73 pKa = 3.29 QNFQQKK79 pKa = 9.05 VVDD82 pKa = 4.39 GLASGINGVVDD93 pKa = 3.77 IANQAVQRR101 pKa = 11.84 EE102 pKa = 4.58 INSRR106 pKa = 11.84 LDD108 pKa = 3.31 PRR110 pKa = 11.84 PPTVVEE116 pKa = 4.43 MEE118 pKa = 4.73 DD119 pKa = 3.33 ATLPPPKK126 pKa = 10.05 GEE128 pKa = 3.9 KK129 pKa = 9.84 RR130 pKa = 11.84 PRR132 pKa = 11.84 PDD134 pKa = 3.14 AEE136 pKa = 4.04 EE137 pKa = 4.59 TILQVDD143 pKa = 4.62 EE144 pKa = 4.54 PPSYY148 pKa = 11.1 EE149 pKa = 4.14 EE150 pKa = 3.88 AVKK153 pKa = 10.86 AGMPTTRR160 pKa = 11.84 IIAPLATGVMKK171 pKa = 10.09 PATLDD176 pKa = 3.54 LPPPPTPAPPKK187 pKa = 9.6 AAPVVQPPPVATAVRR202 pKa = 11.84 RR203 pKa = 11.84 VPARR207 pKa = 11.84 RR208 pKa = 11.84 QAQNWQSTLHH218 pKa = 6.39 SIVGLGVKK226 pKa = 8.75 SLKK229 pKa = 9.96 RR230 pKa = 11.84 RR231 pKa = 11.84 RR232 pKa = 11.84 CYY234 pKa = 10.71
Molecular weight: 25.54 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.04
IPC2_protein 9.092
IPC_protein 9.121
Toseland 10.087
ProMoST 9.677
Dawson 10.204
Bjellqvist 9.823
Wikipedia 10.335
Rodwell 10.657
Grimsley 10.248
Solomon 10.248
Lehninger 10.233
Nozaki 10.058
DTASelect 9.823
Thurlkill 10.087
EMBOSS 10.467
Sillero 10.116
Patrickios 10.394
IPC_peptide 10.262
IPC2_peptide 8.302
IPC2.peptide.svr19 8.144
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
1
8
3971
209
1091
496.4
56.15
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.26 ± 0.645
1.158 ± 0.36
5.767 ± 0.228
6.673 ± 0.679
4.155 ± 0.366
5.54 ± 0.447
1.989 ± 0.381
3.526 ± 0.219
3.651 ± 0.861
9.872 ± 0.364
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.392 ± 0.217
4.382 ± 0.517
6.799 ± 0.378
4.231 ± 0.512
7.958 ± 0.942
6.422 ± 0.554
5.465 ± 0.517
6.724 ± 0.388
1.461 ± 0.187
3.576 ± 0.359
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here