Gray Lodge virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Hapavirus; Gray Lodge hapavirus

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0D3R299|A0A0D3R299_9RHAB Phosphoprotein OS=Gray Lodge virus OX=1272942 PE=4 SV=1
MM1 pKa = 7.77LYY3 pKa = 8.6NTRR6 pKa = 11.84LEE8 pKa = 4.24VMVVGDD14 pKa = 4.38LALDD18 pKa = 3.53TFGAKK23 pKa = 10.27LEE25 pKa = 4.71LFMGMYY31 pKa = 9.63KK32 pKa = 10.22KK33 pKa = 10.81VVGQFKK39 pKa = 10.5DD40 pKa = 4.48ANTLWRR46 pKa = 11.84SSLLMLLANAEE57 pKa = 4.13EE58 pKa = 4.35TQTVNGLNQIHH69 pKa = 6.24CVILKK74 pKa = 10.6LIDD77 pKa = 3.62YY78 pKa = 10.22QGLEE82 pKa = 4.39EE83 pKa = 4.15IQQEE87 pKa = 4.16EE88 pKa = 4.42SFYY91 pKa = 10.49RR92 pKa = 11.84QTSFVPINPGKK103 pKa = 10.2DD104 pKa = 2.8AGAWVILDD112 pKa = 5.12LIPAYY117 pKa = 10.3GSGEE121 pKa = 4.15SCQSIWEE128 pKa = 4.23DD129 pKa = 3.49AEE131 pKa = 4.93SNLSVAKK138 pKa = 10.39YY139 pKa = 10.28LSWDD143 pKa = 3.28EE144 pKa = 3.93ANRR147 pKa = 11.84LFKK150 pKa = 10.8LVEE153 pKa = 3.85

Molecular weight: 17.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0D3R1P1|A0A0D3R1P1_9RHAB Glycoprotein OS=Gray Lodge virus OX=1272942 PE=4 SV=1
MM1 pKa = 7.91GIDD4 pKa = 5.1LKK6 pKa = 11.23INWTEE11 pKa = 3.53LWNSITRR18 pKa = 11.84GLRR21 pKa = 11.84EE22 pKa = 3.92GLDD25 pKa = 3.65FLRR28 pKa = 11.84VWFEE32 pKa = 3.52NFYY35 pKa = 11.34NDD37 pKa = 2.87ARR39 pKa = 11.84KK40 pKa = 8.98WGIIVFIIIITIIIVMIIMKK60 pKa = 9.82ILKK63 pKa = 9.9CVLLIKK69 pKa = 10.06KK70 pKa = 9.38LCKK73 pKa = 10.47SCDD76 pKa = 3.22KK77 pKa = 10.7TSPTRR82 pKa = 11.84GKK84 pKa = 9.43PVRR87 pKa = 11.84KK88 pKa = 9.02IRR90 pKa = 11.84KK91 pKa = 5.02WWRR94 pKa = 11.84KK95 pKa = 8.94KK96 pKa = 9.17VPKK99 pKa = 10.21RR100 pKa = 11.84IQKK103 pKa = 10.33LKK105 pKa = 10.65

Molecular weight: 12.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

4113

99

2106

457.0

52.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.376 ± 0.589

1.678 ± 0.236

5.446 ± 0.454

6.321 ± 0.602

4.498 ± 0.368

5.398 ± 0.289

2.456 ± 0.285

8.023 ± 0.653

6.54 ± 0.509

10.601 ± 0.865

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.529 ± 0.397

5.081 ± 0.425

4.449 ± 0.512

3.331 ± 0.429

5.081 ± 0.334

8.048 ± 0.497

5.106 ± 0.345

5.495 ± 0.539

2.115 ± 0.261

3.428 ± 0.21
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski