Columbid alphaherpesvirus 1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes; Herpesvirales; Herpesviridae; Alphaherpesvirinae; Mardivirus

Average proteome isoelectric point is 7.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 108 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1V0M8E1|A0A1V0M8E1_9ALPH Uracil-DNA glycosylase OS=Columbid alphaherpesvirus 1 OX=93386 GN=CoHVHLJ_011 PE=3 SV=1
MM1 pKa = 7.49SSAIATFITYY11 pKa = 8.14TFVGAKK17 pKa = 8.9TSPTWTVPNYY27 pKa = 9.34EE28 pKa = 4.67QIICSCDD35 pKa = 2.68GGTRR39 pKa = 11.84SVAVGGMSRR48 pKa = 11.84CDD50 pKa = 3.54YY51 pKa = 11.18LPATNVILQRR61 pKa = 11.84GPAGTLLILDD71 pKa = 4.67GGVEE75 pKa = 4.34FCSYY79 pKa = 11.2LLRR82 pKa = 11.84SEE84 pKa = 4.9DD85 pKa = 3.43ATSARR90 pKa = 11.84SSGYY94 pKa = 10.26LSSAPGSICVVPFSSCTVIGLDD116 pKa = 3.77TYY118 pKa = 11.38VCNNSSGVLTVTWLRR133 pKa = 11.84QAAYY137 pKa = 8.21ITLTVYY143 pKa = 10.89GSGHH147 pKa = 6.49EE148 pKa = 4.13YY149 pKa = 10.42RR150 pKa = 11.84ISEE153 pKa = 4.56PGSVSQSSCSSVASQKK169 pKa = 11.14DD170 pKa = 3.49SAAEE174 pKa = 4.04SCEE177 pKa = 3.62GRR179 pKa = 11.84AVPTPEE185 pKa = 4.33NRR187 pKa = 11.84VPDD190 pKa = 4.24DD191 pKa = 3.59AVAEE195 pKa = 4.23ARR197 pKa = 11.84DD198 pKa = 3.84GASAPRR204 pKa = 11.84EE205 pKa = 3.97PDD207 pKa = 3.31DD208 pKa = 4.03VLDD211 pKa = 6.77EE212 pKa = 4.67IIQWNVHH219 pKa = 5.15GPGAGLAGALSPGSTPTQTKK239 pKa = 9.81NGEE242 pKa = 4.15GGGDD246 pKa = 3.56LLAAAMRR253 pKa = 11.84EE254 pKa = 4.03SHH256 pKa = 6.9LGVEE260 pKa = 4.63PLSFSDD266 pKa = 4.89IDD268 pKa = 3.52VDD270 pKa = 5.09LPNIPIPLPAQSPPAIAPVEE290 pKa = 4.3YY291 pKa = 9.98IFQDD295 pKa = 3.07NDD297 pKa = 2.87QPADD301 pKa = 3.86FGFLSSGPP309 pKa = 3.69

Molecular weight: 32.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1V0M8M6|A0A1V0M8M6_9ALPH Virion protein US2 OS=Columbid alphaherpesvirus 1 OX=93386 GN=CoHVHLJ_106 PE=4 SV=1
MM1 pKa = 7.64GLPLRR6 pKa = 11.84RR7 pKa = 11.84SSRR10 pKa = 11.84IRR12 pKa = 11.84GAAGHH17 pKa = 6.12VATALVSRR25 pKa = 11.84LEE27 pKa = 3.92TAARR31 pKa = 11.84YY32 pKa = 9.48ASGGRR37 pKa = 11.84RR38 pKa = 11.84PVTRR42 pKa = 11.84SCAVGRR48 pKa = 11.84LHH50 pKa = 7.31LGVGINNRR58 pKa = 11.84RR59 pKa = 11.84VRR61 pKa = 11.84GGPQTSPSAASQPASAIPASRR82 pKa = 11.84RR83 pKa = 11.84TALGMRR89 pKa = 11.84GVGGVIGEE97 pKa = 4.19VAKK100 pKa = 10.54RR101 pKa = 11.84VAGGEE106 pKa = 4.12RR107 pKa = 11.84AASAPRR113 pKa = 11.84ARR115 pKa = 11.84RR116 pKa = 11.84RR117 pKa = 11.84PRR119 pKa = 11.84SSLNSNAAAAAAAAAATGSDD139 pKa = 4.05APQMVPGQTKK149 pKa = 9.94KK150 pKa = 10.73CGAPKK155 pKa = 10.35RR156 pKa = 11.84STAATTTTTATTADD170 pKa = 4.38GGCSCAGGGAACGAHH185 pKa = 5.78HH186 pKa = 7.35RR187 pKa = 11.84EE188 pKa = 3.98RR189 pKa = 11.84DD190 pKa = 3.12GHH192 pKa = 5.11RR193 pKa = 11.84RR194 pKa = 11.84RR195 pKa = 11.84TTVCMGTCAIGRR207 pKa = 11.84RR208 pKa = 11.84RR209 pKa = 11.84SDD211 pKa = 2.8ASRR214 pKa = 11.84EE215 pKa = 4.31CACMPPPASTTPAGAGAVAAAAVAPCVDD243 pKa = 3.46SRR245 pKa = 11.84TLRR248 pKa = 11.84PQQRR252 pKa = 11.84PHH254 pKa = 5.73PRR256 pKa = 11.84SRR258 pKa = 11.84RR259 pKa = 11.84GTVSPIEE266 pKa = 4.16ASVASPAGPSDD277 pKa = 3.29ADD279 pKa = 3.17YY280 pKa = 11.32RR281 pKa = 11.84RR282 pKa = 11.84LYY284 pKa = 10.29KK285 pKa = 10.52CRR287 pKa = 11.84LDD289 pKa = 4.07TEE291 pKa = 4.18EE292 pKa = 4.87HH293 pKa = 6.18AASLPRR299 pKa = 11.84SVKK302 pKa = 10.16DD303 pKa = 3.34LAHH306 pKa = 6.26ITHH309 pKa = 6.81TAARR313 pKa = 11.84DD314 pKa = 3.33AYY316 pKa = 9.04SAMRR320 pKa = 11.84AGMPPPARR328 pKa = 11.84LWKK331 pKa = 10.32IVGCKK336 pKa = 9.98FKK338 pKa = 11.12ALLQSYY344 pKa = 8.19EE345 pKa = 4.0RR346 pKa = 11.84SAPPFNPSEE355 pKa = 4.63PIRR358 pKa = 11.84TVAAKK363 pKa = 10.35AFRR366 pKa = 11.84AIAAVPPASHH376 pKa = 6.5QEE378 pKa = 3.86LSDD381 pKa = 3.52ALTLVMYY388 pKa = 7.4WCCLGHH394 pKa = 6.77VSRR397 pKa = 11.84CKK399 pKa = 9.78MAGLYY404 pKa = 9.38EE405 pKa = 4.32RR406 pKa = 11.84EE407 pKa = 4.09PAVPALFDD415 pKa = 3.73ARR417 pKa = 11.84PGCGEE422 pKa = 4.26IPPSDD427 pKa = 3.51VNYY430 pKa = 9.64WKK432 pKa = 10.26QLYY435 pKa = 9.96RR436 pKa = 11.84LADD439 pKa = 3.43KK440 pKa = 10.65GGSSIVPDD448 pKa = 4.06ASLAAYY454 pKa = 8.75VALVRR459 pKa = 11.84RR460 pKa = 11.84DD461 pKa = 3.49PDD463 pKa = 3.47EE464 pKa = 4.26EE465 pKa = 4.28LVGG468 pKa = 3.88

Molecular weight: 48.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

108

0

108

47845

77

3280

443.0

48.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.066 ± 0.359

2.067 ± 0.154

6.239 ± 0.201

5.449 ± 0.194

3.424 ± 0.156

7.719 ± 0.252

1.91 ± 0.08

3.944 ± 0.132

3.334 ± 0.158

8.523 ± 0.202

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.278 ± 0.081

2.799 ± 0.134

6.191 ± 0.248

2.558 ± 0.098

8.663 ± 0.258

8.206 ± 0.241

5.792 ± 0.14

6.749 ± 0.157

1.158 ± 0.074

2.932 ± 0.116
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski