Paracoccus lutimaris
Average proteome isoelectric point is 6.42
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3955 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A368YUU5|A0A368YUU5_9RHOB Thiazole synthase OS=Paracoccus lutimaris OX=1490030 GN=thiG PE=3 SV=1
MM1 pKa = 7.5 SSATASATFKK11 pKa = 10.82 LPPDD15 pKa = 3.4 QASPTFEE22 pKa = 5.39 HH23 pKa = 7.03 ISGDD27 pKa = 3.8 GADD30 pKa = 3.25 WLYY33 pKa = 11.58 GGVGNDD39 pKa = 3.84 TLWGGRR45 pKa = 11.84 DD46 pKa = 3.24 EE47 pKa = 5.0 GRR49 pKa = 11.84 LFGGDD54 pKa = 3.39 GNDD57 pKa = 3.74 RR58 pKa = 11.84 LYY60 pKa = 11.09 GYY62 pKa = 10.23 NNHH65 pKa = 7.28 DD66 pKa = 4.13 SLIGGAGNDD75 pKa = 3.74 YY76 pKa = 10.55 MSGGNGDD83 pKa = 3.92 DD84 pKa = 3.84 HH85 pKa = 8.57 CDD87 pKa = 3.89 GEE89 pKa = 4.81 DD90 pKa = 3.66 GNDD93 pKa = 4.21 GIEE96 pKa = 4.28 GAEE99 pKa = 4.19 GTDD102 pKa = 3.15 RR103 pKa = 11.84 IYY105 pKa = 11.02 SGEE108 pKa = 4.21 GGDD111 pKa = 3.33 TIVFRR116 pKa = 11.84 EE117 pKa = 4.25 ASHH120 pKa = 6.07 SAVGAARR127 pKa = 11.84 DD128 pKa = 3.94 VIHH131 pKa = 7.48 DD132 pKa = 4.62 FDD134 pKa = 3.98 TQYY137 pKa = 10.87 DD138 pKa = 4.38 TIQLYY143 pKa = 10.82 EE144 pKa = 4.21 MFDD147 pKa = 3.8 FMVSIPFWNGTSAAAYY163 pKa = 9.12 SVWILALGNNTLVRR177 pKa = 11.84 ADD179 pKa = 3.64 TTGDD183 pKa = 3.18 AVADD187 pKa = 4.32 FEE189 pKa = 5.24 LLLAGTTGVTEE200 pKa = 4.77 DD201 pKa = 3.66 NFIII205 pKa = 4.78
Molecular weight: 21.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.717
IPC2_protein 3.757
IPC_protein 3.783
Toseland 3.554
ProMoST 3.961
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.745
Rodwell 3.605
Grimsley 3.452
Solomon 3.77
Lehninger 3.732
Nozaki 3.897
DTASelect 4.177
Thurlkill 3.617
EMBOSS 3.745
Sillero 3.91
Patrickios 1.888
IPC_peptide 3.77
IPC2_peptide 3.872
IPC2.peptide.svr19 3.793
Protein with the highest isoelectric point:
>tr|A0A368YWI1|A0A368YWI1_9RHOB Uncharacterized protein OS=Paracoccus lutimaris OX=1490030 GN=DFP89_1107 PE=4 SV=1
MM1 pKa = 7.69 SKK3 pKa = 9.01 RR4 pKa = 11.84 TFQPSNLVRR13 pKa = 11.84 ARR15 pKa = 11.84 RR16 pKa = 11.84 HH17 pKa = 4.37 GFRR20 pKa = 11.84 ARR22 pKa = 11.84 MATKK26 pKa = 10.12 GGRR29 pKa = 11.84 RR30 pKa = 11.84 VLNARR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.0 GRR40 pKa = 11.84 KK41 pKa = 8.91 SLSAA45 pKa = 3.86
Molecular weight: 5.14 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.647
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 12.998
DTASelect 12.998
Thurlkill 12.998
EMBOSS 13.51
Sillero 12.998
Patrickios 12.369
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.185
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3955
0
3955
1266796
26
3244
320.3
34.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.16 ± 0.059
0.812 ± 0.012
5.974 ± 0.037
5.538 ± 0.038
3.471 ± 0.025
8.961 ± 0.047
2.052 ± 0.018
5.147 ± 0.029
2.752 ± 0.037
10.303 ± 0.046
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.717 ± 0.021
2.444 ± 0.027
5.516 ± 0.036
3.263 ± 0.022
7.421 ± 0.044
4.905 ± 0.025
5.145 ± 0.031
6.861 ± 0.033
1.494 ± 0.017
2.065 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here