Rhesus adenovirus 65
Average proteome isoelectric point is 6.63
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 23 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2H5AIQ9|A0A2H5AIQ9_9ADEN Short fiber 2 OS=Rhesus adenovirus 65 OX=2064741 PE=3 SV=1
MM1 pKa = 7.52 RR2 pKa = 11.84 LVPEE6 pKa = 4.33 MYY8 pKa = 10.57 GVFCSEE14 pKa = 3.99 TARR17 pKa = 11.84 NSDD20 pKa = 3.56 EE21 pKa = 5.03 LLNTDD26 pKa = 5.24 LLDD29 pKa = 3.99 VPNSPVTSPPSLHH42 pKa = 7.22 DD43 pKa = 4.12 LFDD46 pKa = 4.13 VEE48 pKa = 4.99 VDD50 pKa = 3.74 PPQDD54 pKa = 3.72 PNEE57 pKa = 4.31 DD58 pKa = 3.55 AVNSMFPEE66 pKa = 4.4 CLFEE70 pKa = 4.35 AAEE73 pKa = 4.41 EE74 pKa = 4.91 GSHH77 pKa = 6.54 SSEE80 pKa = 3.55 EE81 pKa = 4.08 SRR83 pKa = 11.84 RR84 pKa = 11.84 GEE86 pKa = 4.04 EE87 pKa = 4.95 LDD89 pKa = 3.54 LKK91 pKa = 10.78 CYY93 pKa = 9.67 EE94 pKa = 4.18 EE95 pKa = 5.03 CLPSSDD101 pKa = 5.28 SEE103 pKa = 4.53 TEE105 pKa = 3.84 QTGGDD110 pKa = 4.02 CCTEE114 pKa = 3.61 PVAKK118 pKa = 10.58 NEE120 pKa = 4.3 LVLDD124 pKa = 4.56 CPDD127 pKa = 3.59 QPGHH131 pKa = 5.94 GCRR134 pKa = 11.84 ACAFHH139 pKa = 7.21 RR140 pKa = 11.84 NASGNPEE147 pKa = 4.12 TLCALCYY154 pKa = 10.44 LRR156 pKa = 11.84 LTSNFVYY163 pKa = 10.73 SKK165 pKa = 11.39
Molecular weight: 18.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.927
IPC2_protein 4.113
IPC_protein 4.05
Toseland 3.884
ProMoST 4.177
Dawson 4.012
Bjellqvist 4.164
Wikipedia 3.884
Rodwell 3.884
Grimsley 3.795
Solomon 3.999
Lehninger 3.961
Nozaki 4.126
DTASelect 4.266
Thurlkill 3.897
EMBOSS 3.91
Sillero 4.164
Patrickios 0.579
IPC_peptide 4.012
IPC2_peptide 4.151
IPC2.peptide.svr19 4.063
Protein with the highest isoelectric point:
>tr|A0A2H5AIS0|A0A2H5AIS0_9ADEN Isoform of A0A2H5AIQ6 Core-capsid bridging protein OS=Rhesus adenovirus 65 OX=2064741 GN=L2 PE=2 SV=1
MM1 pKa = 7.68 SKK3 pKa = 10.58 RR4 pKa = 11.84 KK5 pKa = 9.36 FKK7 pKa = 11.23 EE8 pKa = 3.69 EE9 pKa = 3.77 LLQTLVPEE17 pKa = 4.89 IYY19 pKa = 10.46 GPPDD23 pKa = 3.49 VKK25 pKa = 10.76 PDD27 pKa = 3.26 IKK29 pKa = 10.79 PRR31 pKa = 11.84 DD32 pKa = 3.37 IKK34 pKa = 10.63 RR35 pKa = 11.84 VKK37 pKa = 10.18 KK38 pKa = 10.27 RR39 pKa = 11.84 EE40 pKa = 4.09 KK41 pKa = 10.28 KK42 pKa = 10.22 EE43 pKa = 3.62 EE44 pKa = 3.97 LAVVDD49 pKa = 4.17 DD50 pKa = 4.68 GGVEE54 pKa = 5.24 FIRR57 pKa = 11.84 SFAPRR62 pKa = 11.84 RR63 pKa = 11.84 RR64 pKa = 11.84 VQWKK68 pKa = 7.89 GRR70 pKa = 11.84 RR71 pKa = 11.84 VQRR74 pKa = 11.84 VLRR77 pKa = 11.84 PGTAVVFTPGEE88 pKa = 3.9 RR89 pKa = 11.84 SAVRR93 pKa = 11.84 GFKK96 pKa = 10.07 RR97 pKa = 11.84 QYY99 pKa = 10.65 DD100 pKa = 3.87 EE101 pKa = 5.1 VYY103 pKa = 10.34 GDD105 pKa = 3.95 EE106 pKa = 6.16 DD107 pKa = 3.53 ILEE110 pKa = 4.12 QAAQQIGEE118 pKa = 4.01 FAYY121 pKa = 10.17 GKK123 pKa = 9.96 RR124 pKa = 11.84 SRR126 pKa = 11.84 RR127 pKa = 11.84 GDD129 pKa = 3.34 LAIALDD135 pKa = 4.24 SGNPTPSLKK144 pKa = 10.31 PVTLQQVLPVSASTDD159 pKa = 2.99 SKK161 pKa = 11.03 RR162 pKa = 11.84 GIKK165 pKa = 9.92 RR166 pKa = 11.84 EE167 pKa = 3.97 MEE169 pKa = 4.23 DD170 pKa = 3.79 LQPTIQLMVPKK181 pKa = 9.88 RR182 pKa = 11.84 QRR184 pKa = 11.84 LEE186 pKa = 3.86 EE187 pKa = 4.07 VLEE190 pKa = 4.11 KK191 pKa = 10.62 MKK193 pKa = 10.45 VDD195 pKa = 3.79 PSIEE199 pKa = 4.03 PDD201 pKa = 3.49 VKK203 pKa = 10.46 VRR205 pKa = 11.84 PIKK208 pKa = 10.28 EE209 pKa = 3.96 VAPGLGVQTVDD220 pKa = 2.75 IQIPVTSASTAVEE233 pKa = 3.8 AMEE236 pKa = 4.52 TQTEE240 pKa = 4.42 TPAAIGTRR248 pKa = 11.84 EE249 pKa = 4.14 VALQTDD255 pKa = 3.78 PWYY258 pKa = 10.37 EE259 pKa = 3.65 YY260 pKa = 10.81 AAPRR264 pKa = 11.84 RR265 pKa = 11.84 QRR267 pKa = 11.84 RR268 pKa = 11.84 PARR271 pKa = 11.84 YY272 pKa = 8.94 GPANAIMPEE281 pKa = 4.29 YY282 pKa = 10.19 ALHH285 pKa = 6.62 PSILPTPGYY294 pKa = 10.04 RR295 pKa = 11.84 GVTYY299 pKa = 10.18 RR300 pKa = 11.84 PSGTRR305 pKa = 11.84 RR306 pKa = 11.84 RR307 pKa = 11.84 TRR309 pKa = 11.84 RR310 pKa = 11.84 RR311 pKa = 11.84 RR312 pKa = 11.84 RR313 pKa = 11.84 SRR315 pKa = 11.84 RR316 pKa = 11.84 ALAPVSVRR324 pKa = 11.84 RR325 pKa = 11.84 VTRR328 pKa = 11.84 RR329 pKa = 11.84 GKK331 pKa = 8.55 TVTIPNPRR339 pKa = 11.84 YY340 pKa = 9.63 HH341 pKa = 7.15 PSILL345 pKa = 3.57
Molecular weight: 39.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.315
IPC2_protein 9.663
IPC_protein 10.452
Toseland 10.672
ProMoST 10.365
Dawson 10.774
Bjellqvist 10.467
Wikipedia 10.979
Rodwell 10.994
Grimsley 10.818
Solomon 10.877
Lehninger 10.847
Nozaki 10.643
DTASelect 10.467
Thurlkill 10.672
EMBOSS 11.082
Sillero 10.701
Patrickios 10.687
IPC_peptide 10.891
IPC2_peptide 9.194
IPC2.peptide.svr19 8.765
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
19
4
23
8548
116
931
371.7
41.64
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.698 ± 0.491
1.533 ± 0.315
4.878 ± 0.279
5.592 ± 0.5
4.083 ± 0.23
6.352 ± 0.384
2.0 ± 0.251
3.72 ± 0.205
3.451 ± 0.358
9.815 ± 0.546
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.504 ± 0.205
5.253 ± 0.554
6.82 ± 0.406
4.258 ± 0.237
7.323 ± 0.709
7.183 ± 0.431
5.884 ± 0.405
6.75 ± 0.371
1.299 ± 0.132
3.603 ± 0.321
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here