Chryseobacterium sp. legu1
Average proteome isoelectric point is 6.61
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2570 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5C7EUP0|A0A5C7EUP0_9FLAO YkgJ family cysteine cluster protein OS=Chryseobacterium sp. legu1 OX=2602768 GN=FUA25_09355 PE=4 SV=1
MM1 pKa = 7.49 QDD3 pKa = 2.88 INLKK7 pKa = 8.51 ITDD10 pKa = 3.89 RR11 pKa = 11.84 NGEE14 pKa = 3.81 IHH16 pKa = 6.79 EE17 pKa = 4.61 VVAPTDD23 pKa = 3.19 MSMNLMEE30 pKa = 4.84 VIRR33 pKa = 11.84 SYY35 pKa = 11.43 EE36 pKa = 3.86 LADD39 pKa = 3.65 EE40 pKa = 4.45 GTIGVCGGMAMCASCQVYY58 pKa = 10.18 VLEE61 pKa = 4.71 GSEE64 pKa = 4.35 KK65 pKa = 9.89 LVEE68 pKa = 4.37 MGDD71 pKa = 3.77 EE72 pKa = 4.09 EE73 pKa = 5.23 DD74 pKa = 4.81 AMLAEE79 pKa = 4.64 AFHH82 pKa = 6.09 VQDD85 pKa = 4.36 HH86 pKa = 6.43 SRR88 pKa = 11.84 LGCQIHH94 pKa = 5.78 ITEE97 pKa = 5.18 EE98 pKa = 3.9 IDD100 pKa = 3.52 GLEE103 pKa = 4.24 VAIAPYY109 pKa = 9.81 PP110 pKa = 3.58
Molecular weight: 12.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.866
IPC2_protein 4.126
IPC_protein 4.037
Toseland 3.884
ProMoST 4.164
Dawson 3.999
Bjellqvist 4.151
Wikipedia 3.872
Rodwell 3.884
Grimsley 3.795
Solomon 3.986
Lehninger 3.948
Nozaki 4.113
DTASelect 4.24
Thurlkill 3.897
EMBOSS 3.897
Sillero 4.151
Patrickios 0.998
IPC_peptide 3.999
IPC2_peptide 4.139
IPC2.peptide.svr19 4.045
Protein with the highest isoelectric point:
>tr|A0A5C7F5R0|A0A5C7F5R0_9FLAO Uncharacterized protein OS=Chryseobacterium sp. legu1 OX=2602768 GN=FUA25_06880 PE=4 SV=1
MM1 pKa = 8.0 PKK3 pKa = 9.03 RR4 pKa = 11.84 TFQPSEE10 pKa = 3.58 RR11 pKa = 11.84 KK12 pKa = 9.37 KK13 pKa = 10.2 RR14 pKa = 11.84 NKK16 pKa = 9.71 HH17 pKa = 4.09 GFRR20 pKa = 11.84 EE21 pKa = 4.33 RR22 pKa = 11.84 MSTPNGRR29 pKa = 11.84 RR30 pKa = 11.84 VLAARR35 pKa = 11.84 RR36 pKa = 11.84 AKK38 pKa = 10.02 GRR40 pKa = 11.84 KK41 pKa = 8.75 SLSISSARR49 pKa = 11.84 AKK51 pKa = 10.43 RR52 pKa = 3.43
Molecular weight: 6.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 10.745
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.486
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.223
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 11.959
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.101
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2570
0
2570
821522
25
2325
319.7
36.1
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.593 ± 0.052
0.731 ± 0.014
5.318 ± 0.034
6.827 ± 0.063
5.562 ± 0.047
6.416 ± 0.042
1.74 ± 0.025
7.616 ± 0.052
7.943 ± 0.056
9.234 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.487 ± 0.028
5.927 ± 0.049
3.507 ± 0.027
3.436 ± 0.024
3.57 ± 0.03
6.429 ± 0.046
5.627 ± 0.068
6.126 ± 0.04
1.009 ± 0.017
3.903 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here