Clostridium tarantellae
Average proteome isoelectric point is 6.85
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3225 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6I1MR54|A0A6I1MR54_9CLOT MarR family transcriptional regulator OS=Clostridium tarantellae OX=39493 GN=GBZ86_14555 PE=4 SV=1
MM1 pKa = 7.29 KK2 pKa = 10.47 AVVDD6 pKa = 3.74 KK7 pKa = 10.4 DD8 pKa = 3.67 LCIGCGLCEE17 pKa = 4.25 STCPEE22 pKa = 4.1 VFSIGDD28 pKa = 3.68 DD29 pKa = 3.69 GKK31 pKa = 10.92 SQAISEE37 pKa = 5.0 DD38 pKa = 3.26 IPKK41 pKa = 10.19 EE42 pKa = 3.96 FEE44 pKa = 4.47 DD45 pKa = 3.88 NAVEE49 pKa = 4.6 ASTNCPVGAITIEE62 pKa = 3.94
Molecular weight: 6.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.779
IPC2_protein 3.923
IPC_protein 3.795
Toseland 3.617
ProMoST 3.91
Dawson 3.783
Bjellqvist 4.024
Wikipedia 3.706
Rodwell 3.643
Grimsley 3.541
Solomon 3.757
Lehninger 3.706
Nozaki 3.91
DTASelect 4.062
Thurlkill 3.681
EMBOSS 3.719
Sillero 3.923
Patrickios 1.875
IPC_peptide 3.757
IPC2_peptide 3.897
IPC2.peptide.svr19 3.859
Protein with the highest isoelectric point:
>tr|A0A6I1MID3|A0A6I1MID3_9CLOT Oxidoreductase OS=Clostridium tarantellae OX=39493 GN=GBZ86_02745 PE=4 SV=1
MM1 pKa = 7.53 SRR3 pKa = 11.84 RR4 pKa = 11.84 CEE6 pKa = 3.79 ICDD9 pKa = 3.31 KK10 pKa = 11.1 GVVSGKK16 pKa = 10.1 QYY18 pKa = 10.63 SHH20 pKa = 6.33 SHH22 pKa = 5.27 RR23 pKa = 11.84 QSNRR27 pKa = 11.84 TWAPNIKK34 pKa = 9.58 KK35 pKa = 10.2 VKK37 pKa = 10.08 AIVKK41 pKa = 7.32 GTPKK45 pKa = 9.67 TVHH48 pKa = 4.9 VCTRR52 pKa = 11.84 CLRR55 pKa = 11.84 SGKK58 pKa = 7.37 VQRR61 pKa = 11.84 AII63 pKa = 3.7
Molecular weight: 7.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.311
IPC2_protein 9.78
IPC_protein 10.335
Toseland 11.038
ProMoST 10.672
Dawson 11.082
Bjellqvist 10.76
Wikipedia 11.272
Rodwell 11.447
Grimsley 11.111
Solomon 11.228
Lehninger 11.199
Nozaki 11.023
DTASelect 10.76
Thurlkill 11.008
EMBOSS 11.433
Sillero 11.023
Patrickios 11.199
IPC_peptide 11.242
IPC2_peptide 9.97
IPC2.peptide.svr19 8.653
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3225
0
3225
1102011
19
4519
341.7
38.7
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.987 ± 0.048
1.141 ± 0.018
5.296 ± 0.044
7.315 ± 0.059
4.59 ± 0.036
6.145 ± 0.05
1.299 ± 0.021
10.374 ± 0.058
9.651 ± 0.061
9.067 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.24 ± 0.022
7.756 ± 0.058
2.743 ± 0.03
2.084 ± 0.018
2.86 ± 0.027
6.294 ± 0.04
5.178 ± 0.054
6.032 ± 0.041
0.736 ± 0.018
4.212 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here