uncultured phage_MedDCM-OCT-S28-C10

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Votkovvirus; Votkovvirus S28C10

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6S4P8E6|A0A6S4P8E6_9CAUD Integrase OS=uncultured phage_MedDCM-OCT-S28-C10 OX=2741077 PE=3 SV=1
MM1 pKa = 8.07DD2 pKa = 6.38LLTIFIFGVIDD13 pKa = 3.49NGVLVLAFYY22 pKa = 9.07LTYY25 pKa = 11.17LNLEE29 pKa = 4.33VVTNKK34 pKa = 10.06YY35 pKa = 10.89LKK37 pKa = 10.08ISVSAFLLGVISAGVSNTISDD58 pKa = 3.7SLGFFLQFEE67 pKa = 4.49FLYY70 pKa = 10.99GLIVGLGCLAGMLIIPLMEE89 pKa = 5.15FIQNNKK95 pKa = 9.55KK96 pKa = 10.5SCTDD100 pKa = 3.45DD101 pKa = 3.3SS102 pKa = 4.01

Molecular weight: 11.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6S4PDH2|A0A6S4PDH2_9CAUD DNA primase/helicased OS=uncultured phage_MedDCM-OCT-S28-C10 OX=2741077 PE=4 SV=1
MM1 pKa = 7.35EE2 pKa = 5.47AYY4 pKa = 9.55IYY6 pKa = 10.01KK7 pKa = 10.56ARR9 pKa = 11.84GKK11 pKa = 11.03AMFDD15 pKa = 3.48VVSSFRR21 pKa = 11.84KK22 pKa = 9.26IGNEE26 pKa = 3.31IQAQTMQTFLFIAMCEE42 pKa = 3.94NRR44 pKa = 11.84EE45 pKa = 3.84IAMSSLAQRR54 pKa = 11.84LGMSQASVSRR64 pKa = 11.84NISYY68 pKa = 10.36FMKK71 pKa = 10.18TNRR74 pKa = 11.84LHH76 pKa = 5.35QAGPGFLEE84 pKa = 4.16TRR86 pKa = 11.84EE87 pKa = 4.38DD88 pKa = 3.32PTEE91 pKa = 3.96RR92 pKa = 11.84RR93 pKa = 11.84RR94 pKa = 11.84KK95 pKa = 9.23LVKK98 pKa = 9.32LTNKK102 pKa = 9.16GHH104 pKa = 5.72MFYY107 pKa = 11.17EE108 pKa = 4.35EE109 pKa = 3.96LAGFLLKK116 pKa = 10.33KK117 pKa = 10.57RR118 pKa = 11.84PP119 pKa = 3.46

Molecular weight: 13.79 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

12574

49

1181

237.2

26.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.174 ± 0.545

1.113 ± 0.155

6.164 ± 0.244

6.219 ± 0.482

3.666 ± 0.191

6.696 ± 0.423

1.718 ± 0.175

7.508 ± 0.336

7.921 ± 0.499

8.295 ± 0.392

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.251 ± 0.197

6.251 ± 0.35

3.086 ± 0.192

3.658 ± 0.226

3.778 ± 0.314

7.11 ± 0.442

7.007 ± 0.651

5.344 ± 0.21

1.201 ± 0.12

3.841 ± 0.232
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski