Amino acid dipepetide frequency for uncultured phage_MedDCM-OCT-S28-C10

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.761AlaAla: 6.761 ± 1.57
0.795AlaCys: 0.795 ± 0.205
3.738AlaAsp: 3.738 ± 0.543
4.136AlaGlu: 4.136 ± 0.697
2.545AlaPhe: 2.545 ± 0.487
5.408AlaGly: 5.408 ± 0.677
0.954AlaHis: 0.954 ± 0.27
3.818AlaIle: 3.818 ± 0.545
6.204AlaLys: 6.204 ± 0.803
5.806AlaLeu: 5.806 ± 0.65
1.591AlaMet: 1.591 ± 0.42
4.215AlaAsn: 4.215 ± 0.613
2.386AlaPro: 2.386 ± 0.389
2.545AlaGln: 2.545 ± 0.569
2.227AlaArg: 2.227 ± 0.465
5.727AlaSer: 5.727 ± 1.436
6.363AlaThr: 6.363 ± 1.747
3.181AlaVal: 3.181 ± 0.522
0.795AlaTrp: 0.795 ± 0.251
2.545AlaTyr: 2.545 ± 0.569
0.0AlaXaa: 0.0 ± 0.0
Cys
0.398CysAla: 0.398 ± 0.168
0.239CysCys: 0.239 ± 0.15
0.318CysAsp: 0.318 ± 0.169
0.875CysGlu: 0.875 ± 0.246
0.795CysPhe: 0.795 ± 0.34
0.636CysGly: 0.636 ± 0.24
0.08CysHis: 0.08 ± 0.072
0.875CysIle: 0.875 ± 0.275
0.795CysLys: 0.795 ± 0.258
0.875CysLeu: 0.875 ± 0.278
0.318CysMet: 0.318 ± 0.229
0.795CysAsn: 0.795 ± 0.238
0.557CysPro: 0.557 ± 0.189
0.318CysGln: 0.318 ± 0.148
0.557CysArg: 0.557 ± 0.229
0.398CysSer: 0.398 ± 0.145
1.113CysThr: 1.113 ± 0.267
0.557CysVal: 0.557 ± 0.201
0.159CysTrp: 0.159 ± 0.12
0.477CysTyr: 0.477 ± 0.189
0.0CysXaa: 0.0 ± 0.0
Asp
4.931AspAla: 4.931 ± 0.805
0.716AspCys: 0.716 ± 0.273
3.261AspAsp: 3.261 ± 0.487
3.34AspGlu: 3.34 ± 0.521
2.625AspPhe: 2.625 ± 0.41
4.136AspGly: 4.136 ± 0.68
0.716AspHis: 0.716 ± 0.201
4.534AspIle: 4.534 ± 0.621
5.488AspLys: 5.488 ± 0.687
6.442AspLeu: 6.442 ± 0.586
1.034AspMet: 1.034 ± 0.21
3.34AspAsn: 3.34 ± 0.625
2.545AspPro: 2.545 ± 0.496
2.068AspGln: 2.068 ± 0.458
2.307AspArg: 2.307 ± 0.393
3.818AspSer: 3.818 ± 0.451
3.659AspThr: 3.659 ± 0.427
3.579AspVal: 3.579 ± 0.574
0.318AspTrp: 0.318 ± 0.193
2.784AspTyr: 2.784 ± 0.474
0.0AspXaa: 0.0 ± 0.0
Glu
4.693GluAla: 4.693 ± 0.586
0.557GluCys: 0.557 ± 0.238
2.704GluAsp: 2.704 ± 0.513
5.647GluGlu: 5.647 ± 1.145
2.704GluPhe: 2.704 ± 0.423
3.102GluGly: 3.102 ± 0.324
0.636GluHis: 0.636 ± 0.192
4.056GluIle: 4.056 ± 0.978
4.693GluLys: 4.693 ± 0.687
7.238GluLeu: 7.238 ± 0.882
2.068GluMet: 2.068 ± 0.399
3.42GluAsn: 3.42 ± 0.496
1.511GluPro: 1.511 ± 0.4
3.022GluGln: 3.022 ± 0.48
2.625GluArg: 2.625 ± 0.521
3.5GluSer: 3.5 ± 0.61
3.738GluThr: 3.738 ± 0.503
2.784GluVal: 2.784 ± 0.462
0.636GluTrp: 0.636 ± 0.215
2.863GluTyr: 2.863 ± 0.561
0.0GluXaa: 0.0 ± 0.0
Phe
1.432PheAla: 1.432 ± 0.428
0.477PheCys: 0.477 ± 0.177
3.261PheAsp: 3.261 ± 0.475
2.386PheGlu: 2.386 ± 0.429
1.273PhePhe: 1.273 ± 0.316
2.784PheGly: 2.784 ± 0.505
0.398PheHis: 0.398 ± 0.173
2.625PheIle: 2.625 ± 0.387
2.545PheLys: 2.545 ± 0.447
3.977PheLeu: 3.977 ± 0.602
0.716PheMet: 0.716 ± 0.236
2.466PheAsn: 2.466 ± 0.439
0.716PhePro: 0.716 ± 0.225
1.113PheGln: 1.113 ± 0.321
1.988PheArg: 1.988 ± 0.314
2.545PheSer: 2.545 ± 0.435
2.625PheThr: 2.625 ± 0.448
1.75PheVal: 1.75 ± 0.326
0.239PheTrp: 0.239 ± 0.106
1.352PheTyr: 1.352 ± 0.283
0.0PheXaa: 0.0 ± 0.0
Gly
4.693GlyAla: 4.693 ± 0.624
0.716GlyCys: 0.716 ± 0.252
3.977GlyAsp: 3.977 ± 0.641
2.784GlyGlu: 2.784 ± 0.499
3.261GlyPhe: 3.261 ± 0.671
4.374GlyGly: 4.374 ± 0.491
1.67GlyHis: 1.67 ± 0.426
5.09GlyIle: 5.09 ± 0.486
5.249GlyLys: 5.249 ± 0.704
5.011GlyLeu: 5.011 ± 0.545
1.909GlyMet: 1.909 ± 0.414
3.977GlyAsn: 3.977 ± 0.683
1.591GlyPro: 1.591 ± 0.378
1.67GlyGln: 1.67 ± 0.375
3.022GlyArg: 3.022 ± 0.481
5.965GlySer: 5.965 ± 0.948
5.011GlyThr: 5.011 ± 0.921
3.42GlyVal: 3.42 ± 0.663
1.034GlyTrp: 1.034 ± 0.272
2.545GlyTyr: 2.545 ± 0.341
0.0GlyXaa: 0.0 ± 0.0
His
0.875HisAla: 0.875 ± 0.223
0.239HisCys: 0.239 ± 0.127
0.954HisAsp: 0.954 ± 0.319
0.636HisGlu: 0.636 ± 0.229
0.716HisPhe: 0.716 ± 0.248
0.716HisGly: 0.716 ± 0.19
0.318HisHis: 0.318 ± 0.14
1.909HisIle: 1.909 ± 0.388
1.591HisLys: 1.591 ± 0.382
1.909HisLeu: 1.909 ± 0.383
0.477HisMet: 0.477 ± 0.197
1.113HisAsn: 1.113 ± 0.263
0.716HisPro: 0.716 ± 0.193
0.557HisGln: 0.557 ± 0.244
0.636HisArg: 0.636 ± 0.198
0.795HisSer: 0.795 ± 0.274
1.352HisThr: 1.352 ± 0.407
0.398HisVal: 0.398 ± 0.181
0.557HisTrp: 0.557 ± 0.195
0.716HisTyr: 0.716 ± 0.282
0.0HisXaa: 0.0 ± 0.0
Ile
6.681IleAla: 6.681 ± 0.921
0.795IleCys: 0.795 ± 0.264
5.329IleAsp: 5.329 ± 0.6
5.806IleGlu: 5.806 ± 0.716
2.545IlePhe: 2.545 ± 0.573
5.488IleGly: 5.488 ± 0.673
2.227IleHis: 2.227 ± 0.455
6.045IleIle: 6.045 ± 0.763
6.283IleLys: 6.283 ± 0.652
5.408IleLeu: 5.408 ± 0.692
1.352IleMet: 1.352 ± 0.387
3.977IleAsn: 3.977 ± 0.627
2.943IlePro: 2.943 ± 0.476
2.625IleGln: 2.625 ± 0.505
1.909IleArg: 1.909 ± 0.43
4.136IleSer: 4.136 ± 0.541
4.534IleThr: 4.534 ± 0.669
3.579IleVal: 3.579 ± 0.527
0.636IleTrp: 0.636 ± 0.213
2.784IleTyr: 2.784 ± 0.501
0.0IleXaa: 0.0 ± 0.0
Lys
5.09LysAla: 5.09 ± 0.606
0.636LysCys: 0.636 ± 0.212
4.454LysAsp: 4.454 ± 0.721
6.283LysGlu: 6.283 ± 0.749
1.75LysPhe: 1.75 ± 0.378
4.534LysGly: 4.534 ± 0.797
1.591LysHis: 1.591 ± 0.309
6.204LysIle: 6.204 ± 0.725
6.363LysLys: 6.363 ± 0.975
6.442LysLeu: 6.442 ± 0.646
2.068LysMet: 2.068 ± 0.475
4.534LysAsn: 4.534 ± 0.653
3.34LysPro: 3.34 ± 0.54
2.943LysGln: 2.943 ± 0.489
3.738LysArg: 3.738 ± 0.567
4.852LysSer: 4.852 ± 0.798
4.613LysThr: 4.613 ± 0.511
4.374LysVal: 4.374 ± 0.441
1.67LysTrp: 1.67 ± 0.366
3.659LysTyr: 3.659 ± 0.575
0.0LysXaa: 0.0 ± 0.0
Leu
6.84LeuAla: 6.84 ± 0.882
0.875LeuCys: 0.875 ± 0.258
6.601LeuAsp: 6.601 ± 0.591
5.965LeuGlu: 5.965 ± 0.788
2.466LeuPhe: 2.466 ± 0.47
4.693LeuGly: 4.693 ± 0.529
1.113LeuHis: 1.113 ± 0.328
6.283LeuIle: 6.283 ± 0.911
8.113LeuLys: 8.113 ± 0.867
5.249LeuLeu: 5.249 ± 0.998
1.909LeuMet: 1.909 ± 0.475
3.977LeuAsn: 3.977 ± 0.472
2.704LeuPro: 2.704 ± 0.58
3.022LeuGln: 3.022 ± 0.383
3.818LeuArg: 3.818 ± 0.562
5.408LeuSer: 5.408 ± 0.565
5.567LeuThr: 5.567 ± 0.764
4.613LeuVal: 4.613 ± 0.485
0.875LeuTrp: 0.875 ± 0.236
2.863LeuTyr: 2.863 ± 0.45
0.0LeuXaa: 0.0 ± 0.0
Met
2.068MetAla: 2.068 ± 0.504
0.557MetCys: 0.557 ± 0.231
1.829MetAsp: 1.829 ± 0.403
0.954MetGlu: 0.954 ± 0.239
0.557MetPhe: 0.557 ± 0.247
1.432MetGly: 1.432 ± 0.331
0.398MetHis: 0.398 ± 0.19
1.273MetIle: 1.273 ± 0.285
1.432MetLys: 1.432 ± 0.341
1.829MetLeu: 1.829 ± 0.403
0.477MetMet: 0.477 ± 0.172
1.273MetAsn: 1.273 ± 0.28
0.636MetPro: 0.636 ± 0.243
1.113MetGln: 1.113 ± 0.325
0.875MetArg: 0.875 ± 0.214
2.068MetSer: 2.068 ± 0.418
1.511MetThr: 1.511 ± 0.317
1.432MetVal: 1.432 ± 0.414
0.08MetTrp: 0.08 ± 0.071
0.716MetTyr: 0.716 ± 0.227
0.0MetXaa: 0.0 ± 0.0
Asn
4.374AsnAla: 4.374 ± 0.941
0.716AsnCys: 0.716 ± 0.247
2.784AsnAsp: 2.784 ± 0.422
3.659AsnGlu: 3.659 ± 0.575
2.704AsnPhe: 2.704 ± 0.455
5.886AsnGly: 5.886 ± 1.025
1.113AsnHis: 1.113 ± 0.264
5.011AsnIle: 5.011 ± 0.576
4.295AsnLys: 4.295 ± 0.525
4.534AsnLeu: 4.534 ± 0.678
1.113AsnMet: 1.113 ± 0.32
4.215AsnAsn: 4.215 ± 0.652
2.227AsnPro: 2.227 ± 0.51
2.704AsnGln: 2.704 ± 0.679
2.545AsnArg: 2.545 ± 0.435
4.136AsnSer: 4.136 ± 0.718
4.295AsnThr: 4.295 ± 0.635
2.704AsnVal: 2.704 ± 0.35
0.795AsnTrp: 0.795 ± 0.265
2.704AsnTyr: 2.704 ± 0.424
0.0AsnXaa: 0.0 ± 0.0
Pro
1.829ProAla: 1.829 ± 0.335
0.398ProCys: 0.398 ± 0.196
1.988ProAsp: 1.988 ± 0.433
2.147ProGlu: 2.147 ± 0.446
1.432ProPhe: 1.432 ± 0.227
1.829ProGly: 1.829 ± 0.349
0.477ProHis: 0.477 ± 0.164
2.545ProIle: 2.545 ± 0.576
2.704ProLys: 2.704 ± 0.51
1.511ProLeu: 1.511 ± 0.361
0.875ProMet: 0.875 ± 0.263
2.704ProAsn: 2.704 ± 0.426
0.795ProPro: 0.795 ± 0.218
0.954ProGln: 0.954 ± 0.229
0.398ProArg: 0.398 ± 0.182
2.466ProSer: 2.466 ± 0.46
2.068ProThr: 2.068 ± 0.391
1.909ProVal: 1.909 ± 0.401
0.557ProTrp: 0.557 ± 0.179
1.273ProTyr: 1.273 ± 0.324
0.0ProXaa: 0.0 ± 0.0
Gln
3.261GlnAla: 3.261 ± 0.503
0.398GlnCys: 0.398 ± 0.16
1.67GlnAsp: 1.67 ± 0.4
1.988GlnGlu: 1.988 ± 0.588
1.511GlnPhe: 1.511 ± 0.327
1.511GlnGly: 1.511 ± 0.439
0.477GlnHis: 0.477 ± 0.177
2.863GlnIle: 2.863 ± 0.436
2.704GlnLys: 2.704 ± 0.506
3.897GlnLeu: 3.897 ± 0.622
0.875GlnMet: 0.875 ± 0.309
1.67GlnAsn: 1.67 ± 0.318
0.398GlnPro: 0.398 ± 0.156
1.988GlnGln: 1.988 ± 0.642
1.511GlnArg: 1.511 ± 0.42
2.943GlnSer: 2.943 ± 0.44
2.625GlnThr: 2.625 ± 0.557
2.227GlnVal: 2.227 ± 0.482
0.716GlnTrp: 0.716 ± 0.236
1.352GlnTyr: 1.352 ± 0.358
0.0GlnXaa: 0.0 ± 0.0
Arg
2.068ArgAla: 2.068 ± 0.379
0.239ArgCys: 0.239 ± 0.16
1.988ArgAsp: 1.988 ± 0.464
2.386ArgGlu: 2.386 ± 0.558
1.352ArgPhe: 1.352 ± 0.379
2.545ArgGly: 2.545 ± 0.564
0.795ArgHis: 0.795 ± 0.307
3.34ArgIle: 3.34 ± 0.535
2.863ArgLys: 2.863 ± 0.547
3.818ArgLeu: 3.818 ± 0.513
0.795ArgMet: 0.795 ± 0.235
1.67ArgAsn: 1.67 ± 0.342
1.193ArgPro: 1.193 ± 0.272
1.352ArgGln: 1.352 ± 0.308
2.147ArgArg: 2.147 ± 0.427
2.863ArgSer: 2.863 ± 0.47
2.386ArgThr: 2.386 ± 0.428
2.068ArgVal: 2.068 ± 0.403
0.318ArgTrp: 0.318 ± 0.155
1.591ArgTyr: 1.591 ± 0.491
0.0ArgXaa: 0.0 ± 0.0
Ser
4.613SerAla: 4.613 ± 1.236
0.795SerCys: 0.795 ± 0.282
5.17SerAsp: 5.17 ± 0.637
3.102SerGlu: 3.102 ± 0.367
1.67SerPhe: 1.67 ± 0.403
6.124SerGly: 6.124 ± 0.732
1.591SerHis: 1.591 ± 0.332
5.249SerIle: 5.249 ± 0.657
5.727SerLys: 5.727 ± 0.766
5.727SerLeu: 5.727 ± 0.742
1.034SerMet: 1.034 ± 0.221
5.408SerAsn: 5.408 ± 0.668
1.432SerPro: 1.432 ± 0.246
2.386SerGln: 2.386 ± 0.365
2.068SerArg: 2.068 ± 0.387
6.363SerSer: 6.363 ± 1.349
4.852SerThr: 4.852 ± 1.158
4.136SerVal: 4.136 ± 0.56
0.716SerTrp: 0.716 ± 0.195
2.943SerTyr: 2.943 ± 0.45
0.0SerXaa: 0.0 ± 0.0
Thr
4.295ThrAla: 4.295 ± 0.897
0.875ThrCys: 0.875 ± 0.376
4.215ThrAsp: 4.215 ± 0.647
4.136ThrGlu: 4.136 ± 0.678
2.704ThrPhe: 2.704 ± 0.459
5.408ThrGly: 5.408 ± 0.905
1.113ThrHis: 1.113 ± 0.297
5.647ThrIle: 5.647 ± 0.759
4.454ThrLys: 4.454 ± 0.661
4.931ThrLeu: 4.931 ± 0.638
0.795ThrMet: 0.795 ± 0.235
5.965ThrAsn: 5.965 ± 0.802
2.147ThrPro: 2.147 ± 0.412
2.863ThrGln: 2.863 ± 0.39
1.75ThrArg: 1.75 ± 0.352
5.329ThrSer: 5.329 ± 0.927
6.124ThrThr: 6.124 ± 1.123
4.693ThrVal: 4.693 ± 0.737
0.318ThrTrp: 0.318 ± 0.149
2.307ThrTyr: 2.307 ± 0.508
0.0ThrXaa: 0.0 ± 0.0
Val
3.659ValAla: 3.659 ± 0.678
0.318ValCys: 0.318 ± 0.131
3.42ValAsp: 3.42 ± 0.617
2.943ValGlu: 2.943 ± 0.574
1.75ValPhe: 1.75 ± 0.258
3.5ValGly: 3.5 ± 0.516
0.795ValHis: 0.795 ± 0.245
3.5ValIle: 3.5 ± 0.64
3.738ValLys: 3.738 ± 0.535
4.215ValLeu: 4.215 ± 0.58
1.75ValMet: 1.75 ± 0.333
3.897ValAsn: 3.897 ± 0.459
1.67ValPro: 1.67 ± 0.297
1.829ValGln: 1.829 ± 0.357
1.909ValArg: 1.909 ± 0.327
4.852ValSer: 4.852 ± 0.774
4.534ValThr: 4.534 ± 0.669
2.943ValVal: 2.943 ± 0.508
0.716ValTrp: 0.716 ± 0.231
1.511ValTyr: 1.511 ± 0.338
0.0ValXaa: 0.0 ± 0.0
Trp
0.398TrpAla: 0.398 ± 0.177
0.159TrpCys: 0.159 ± 0.113
1.034TrpAsp: 1.034 ± 0.238
0.318TrpGlu: 0.318 ± 0.178
0.398TrpPhe: 0.398 ± 0.294
0.477TrpGly: 0.477 ± 0.161
0.318TrpHis: 0.318 ± 0.138
1.511TrpIle: 1.511 ± 0.343
0.875TrpLys: 0.875 ± 0.231
1.273TrpLeu: 1.273 ± 0.378
0.08TrpMet: 0.08 ± 0.071
1.193TrpAsn: 1.193 ± 0.313
0.398TrpPro: 0.398 ± 0.163
0.159TrpGln: 0.159 ± 0.1
0.636TrpArg: 0.636 ± 0.254
0.636TrpSer: 0.636 ± 0.243
0.795TrpThr: 0.795 ± 0.203
0.716TrpVal: 0.716 ± 0.18
0.318TrpTrp: 0.318 ± 0.151
0.318TrpTyr: 0.318 ± 0.18
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.784TyrAla: 2.784 ± 0.513
0.636TyrCys: 0.636 ± 0.216
2.943TyrAsp: 2.943 ± 0.401
2.704TyrGlu: 2.704 ± 0.548
1.909TyrPhe: 1.909 ± 0.406
2.386TyrGly: 2.386 ± 0.351
0.398TyrHis: 0.398 ± 0.16
2.704TyrIle: 2.704 ± 0.449
3.102TyrLys: 3.102 ± 0.565
2.863TyrLeu: 2.863 ± 0.38
1.193TyrMet: 1.193 ± 0.257
2.704TyrAsn: 2.704 ± 0.481
0.954TyrPro: 0.954 ± 0.32
1.352TyrGln: 1.352 ± 0.343
1.113TyrArg: 1.113 ± 0.303
2.307TyrSer: 2.307 ± 0.344
2.307TyrThr: 2.307 ± 0.484
2.386TyrVal: 2.386 ± 0.447
0.557TyrTrp: 0.557 ± 0.193
1.113TyrTyr: 1.113 ± 0.382
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 53 proteins (12574 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski