Acidilobus saccharovorans (strain DSM 16705 / JCM 18335 / VKM B-2471 / 345-15)

Taxonomy: cellular organisms; Archaea; TACK group; Crenarchaeota; Thermoprotei; Acidilobales; Acidilobaceae; Acidilobus; Acidilobus saccharovorans

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1499 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D9Q1A6|D9Q1A6_ACIS3 Uncharacterized protein OS=Acidilobus saccharovorans (strain DSM 16705 / JCM 18335 / VKM B-2471 / 345-15) OX=666510 GN=ASAC_0688 PE=4 SV=1
MM1 pKa = 7.79SDD3 pKa = 2.62IQMTIIEE10 pKa = 4.48PSGEE14 pKa = 3.86ADD16 pKa = 3.36YY17 pKa = 11.67DD18 pKa = 3.84EE19 pKa = 4.87SAVLIADD26 pKa = 3.69EE27 pKa = 4.75LKK29 pKa = 10.84AIGLNVVPEE38 pKa = 4.18TLPFSTWFSDD48 pKa = 4.78LLSGDD53 pKa = 3.14YY54 pKa = 10.37WMARR58 pKa = 11.84FWGMGAVPVAAQQFLARR75 pKa = 11.84LSPKK79 pKa = 7.65TTNEE83 pKa = 3.6SAMGFGGFMWINLSQYY99 pKa = 11.18PFMEE103 pKa = 4.8YY104 pKa = 9.88INEE107 pKa = 3.9AMQYY111 pKa = 10.35

Molecular weight: 12.46 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D9Q2J8|D9Q2J8_ACIS3 Flavin-dependent thymidylate synthase OS=Acidilobus saccharovorans (strain DSM 16705 / JCM 18335 / VKM B-2471 / 345-15) OX=666510 GN=thyX PE=3 SV=1
MM1 pKa = 7.42SRR3 pKa = 11.84HH4 pKa = 5.71KK5 pKa = 10.51PLARR9 pKa = 11.84KK10 pKa = 9.1LRR12 pKa = 11.84LAKK15 pKa = 10.23AFKK18 pKa = 10.75SNQPVPAWVIVKK30 pKa = 7.35TLRR33 pKa = 11.84KK34 pKa = 8.41FTLNPRR40 pKa = 11.84RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.19WRR45 pKa = 11.84VSKK48 pKa = 11.03LKK50 pKa = 10.53VV51 pKa = 3.01

Molecular weight: 6.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1499

0

1499

449347

38

1384

299.8

32.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.634 ± 0.076

0.64 ± 0.02

4.663 ± 0.055

6.43 ± 0.077

3.304 ± 0.041

8.099 ± 0.051

1.335 ± 0.024

5.623 ± 0.049

4.302 ± 0.07

10.779 ± 0.069

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.517 ± 0.026

2.838 ± 0.043

4.965 ± 0.042

2.221 ± 0.033

6.517 ± 0.088

7.287 ± 0.067

4.47 ± 0.057

9.156 ± 0.052

1.227 ± 0.026

3.993 ± 0.049
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski