Amino acid dipepetide frequency for Acidilobus saccharovorans (strain DSM 16705 / JCM 18335 / VKM B-2471 / 345-15)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.916AlaAla: 10.916 ± 0.198
0.59AlaCys: 0.59 ± 0.038
3.478AlaAsp: 3.478 ± 0.101
5.906AlaGlu: 5.906 ± 0.144
3.61AlaPhe: 3.61 ± 0.109
6.868AlaGly: 6.868 ± 0.129
1.184AlaHis: 1.184 ± 0.046
6.013AlaIle: 6.013 ± 0.12
4.242AlaLys: 4.242 ± 0.098
10.905AlaLeu: 10.905 ± 0.202
2.824AlaMet: 2.824 ± 0.082
2.259AlaAsn: 2.259 ± 0.07
3.483AlaPro: 3.483 ± 0.092
2.176AlaGln: 2.176 ± 0.081
6.038AlaArg: 6.038 ± 0.152
7.92AlaSer: 7.92 ± 0.165
4.046AlaThr: 4.046 ± 0.104
8.746AlaVal: 8.746 ± 0.148
1.235AlaTrp: 1.235 ± 0.063
3.901AlaTyr: 3.901 ± 0.117
0.0AlaXaa: 0.0 ± 0.0
Cys
0.445CysAla: 0.445 ± 0.031
0.078CysCys: 0.078 ± 0.012
0.369CysAsp: 0.369 ± 0.031
0.392CysGlu: 0.392 ± 0.034
0.145CysPhe: 0.145 ± 0.018
0.884CysGly: 0.884 ± 0.053
0.1CysHis: 0.1 ± 0.016
0.287CysIle: 0.287 ± 0.026
0.205CysLys: 0.205 ± 0.022
0.387CysLeu: 0.387 ± 0.03
0.116CysMet: 0.116 ± 0.014
0.2CysAsn: 0.2 ± 0.021
0.652CysPro: 0.652 ± 0.048
0.113CysGln: 0.113 ± 0.016
0.454CysArg: 0.454 ± 0.031
0.543CysSer: 0.543 ± 0.039
0.265CysThr: 0.265 ± 0.028
0.463CysVal: 0.463 ± 0.035
0.069CysTrp: 0.069 ± 0.014
0.229CysTyr: 0.229 ± 0.024
0.0CysXaa: 0.0 ± 0.0
Asp
3.797AspAla: 3.797 ± 0.109
0.269AspCys: 0.269 ± 0.023
2.248AspAsp: 2.248 ± 0.078
3.576AspGlu: 3.576 ± 0.097
1.687AspPhe: 1.687 ± 0.066
3.291AspGly: 3.291 ± 0.098
0.588AspHis: 0.588 ± 0.039
2.995AspIle: 2.995 ± 0.087
1.916AspLys: 1.916 ± 0.069
5.466AspLeu: 5.466 ± 0.121
1.186AspMet: 1.186 ± 0.055
1.289AspAsn: 1.289 ± 0.068
3.018AspPro: 3.018 ± 0.093
0.763AspGln: 0.763 ± 0.042
2.675AspArg: 2.675 ± 0.076
2.668AspSer: 2.668 ± 0.079
1.413AspThr: 1.413 ± 0.059
5.223AspVal: 5.223 ± 0.118
0.556AspTrp: 0.556 ± 0.038
2.007AspTyr: 2.007 ± 0.07
0.0AspXaa: 0.0 ± 0.0
Glu
7.711GluAla: 7.711 ± 0.154
0.381GluCys: 0.381 ± 0.032
3.153GluAsp: 3.153 ± 0.088
5.323GluGlu: 5.323 ± 0.15
1.863GluPhe: 1.863 ± 0.07
5.499GluGly: 5.499 ± 0.118
0.823GluHis: 0.823 ± 0.049
2.889GluIle: 2.889 ± 0.088
2.633GluLys: 2.633 ± 0.092
7.736GluLeu: 7.736 ± 0.151
1.262GluMet: 1.262 ± 0.055
1.382GluAsn: 1.382 ± 0.059
2.788GluPro: 2.788 ± 0.07
1.509GluGln: 1.509 ± 0.06
4.095GluArg: 4.095 ± 0.11
3.783GluSer: 3.783 ± 0.107
2.03GluThr: 2.03 ± 0.069
6.585GluVal: 6.585 ± 0.129
0.759GluTrp: 0.759 ± 0.042
2.101GluTyr: 2.101 ± 0.077
0.0GluXaa: 0.0 ± 0.0
Phe
2.577PheAla: 2.577 ± 0.09
0.176PheCys: 0.176 ± 0.022
1.796PheAsp: 1.796 ± 0.064
1.745PheGlu: 1.745 ± 0.057
1.246PhePhe: 1.246 ± 0.062
2.424PheGly: 2.424 ± 0.084
0.556PheHis: 0.556 ± 0.037
2.339PheIle: 2.339 ± 0.077
1.533PheLys: 1.533 ± 0.052
3.116PheLeu: 3.116 ± 0.082
1.053PheMet: 1.053 ± 0.057
1.355PheAsn: 1.355 ± 0.062
1.413PhePro: 1.413 ± 0.061
0.596PheGln: 0.596 ± 0.042
1.961PheArg: 1.961 ± 0.07
2.535PheSer: 2.535 ± 0.084
1.965PheThr: 1.965 ± 0.072
2.775PheVal: 2.775 ± 0.089
0.367PheTrp: 0.367 ± 0.03
1.511PheTyr: 1.511 ± 0.064
0.0PheXaa: 0.0 ± 0.0
Gly
6.739GlyAla: 6.739 ± 0.126
0.654GlyCys: 0.654 ± 0.041
3.888GlyAsp: 3.888 ± 0.101
4.616GlyGlu: 4.616 ± 0.094
3.178GlyPhe: 3.178 ± 0.087
6.316GlyGly: 6.316 ± 0.131
1.264GlyHis: 1.264 ± 0.055
4.431GlyIle: 4.431 ± 0.106
3.53GlyLys: 3.53 ± 0.096
10.084GlyLeu: 10.084 ± 0.17
1.898GlyMet: 1.898 ± 0.066
2.132GlyAsn: 2.132 ± 0.077
3.63GlyPro: 3.63 ± 0.116
1.956GlyGln: 1.956 ± 0.078
5.085GlyArg: 5.085 ± 0.124
5.773GlySer: 5.773 ± 0.141
3.614GlyThr: 3.614 ± 0.095
7.636GlyVal: 7.636 ± 0.169
1.142GlyTrp: 1.142 ± 0.058
3.421GlyTyr: 3.421 ± 0.099
0.0GlyXaa: 0.0 ± 0.0
His
1.059HisAla: 1.059 ± 0.055
0.1HisCys: 0.1 ± 0.014
0.712HisAsp: 0.712 ± 0.044
0.81HisGlu: 0.81 ± 0.049
0.476HisPhe: 0.476 ± 0.035
1.358HisGly: 1.358 ± 0.052
0.263HisHis: 0.263 ± 0.028
0.77HisIle: 0.77 ± 0.043
0.372HisLys: 0.372 ± 0.029
1.242HisLeu: 1.242 ± 0.056
0.376HisMet: 0.376 ± 0.027
0.403HisAsn: 0.403 ± 0.028
0.872HisPro: 0.872 ± 0.043
0.245HisGln: 0.245 ± 0.023
0.884HisArg: 0.884 ± 0.04
0.741HisSer: 0.741 ± 0.043
0.55HisThr: 0.55 ± 0.037
1.355HisVal: 1.355 ± 0.061
0.178HisTrp: 0.178 ± 0.024
0.583HisTyr: 0.583 ± 0.039
0.0HisXaa: 0.0 ± 0.0
Ile
6.151IleAla: 6.151 ± 0.124
0.269IleCys: 0.269 ± 0.024
3.073IleAsp: 3.073 ± 0.091
3.492IleGlu: 3.492 ± 0.087
1.551IlePhe: 1.551 ± 0.065
4.197IleGly: 4.197 ± 0.1
0.681IleHis: 0.681 ± 0.047
4.11IleIle: 4.11 ± 0.106
2.624IleLys: 2.624 ± 0.089
3.948IleLeu: 3.948 ± 0.098
1.587IleMet: 1.587 ± 0.058
2.136IleAsn: 2.136 ± 0.081
2.493IlePro: 2.493 ± 0.079
0.781IleGln: 0.781 ± 0.041
3.316IleArg: 3.316 ± 0.092
4.033IleSer: 4.033 ± 0.097
3.136IleThr: 3.136 ± 0.082
5.846IleVal: 5.846 ± 0.117
0.405IleTrp: 0.405 ± 0.032
2.403IleTyr: 2.403 ± 0.076
0.0IleXaa: 0.0 ± 0.0
Lys
4.398LysAla: 4.398 ± 0.103
0.298LysCys: 0.298 ± 0.028
2.241LysAsp: 2.241 ± 0.071
3.469LysGlu: 3.469 ± 0.102
1.364LysPhe: 1.364 ± 0.064
3.875LysGly: 3.875 ± 0.107
0.465LysHis: 0.465 ± 0.031
2.07LysIle: 2.07 ± 0.086
1.662LysLys: 1.662 ± 0.083
4.727LysLeu: 4.727 ± 0.126
0.886LysMet: 0.886 ± 0.054
0.955LysAsn: 0.955 ± 0.047
1.956LysPro: 1.956 ± 0.07
0.852LysGln: 0.852 ± 0.048
2.379LysArg: 2.379 ± 0.08
2.461LysSer: 2.461 ± 0.091
1.464LysThr: 1.464 ± 0.06
5.176LysVal: 5.176 ± 0.122
0.425LysTrp: 0.425 ± 0.031
1.898LysTyr: 1.898 ± 0.069
0.0LysXaa: 0.0 ± 0.0
Leu
11.165LeuAla: 11.165 ± 0.212
0.576LeuCys: 0.576 ± 0.044
4.678LeuAsp: 4.678 ± 0.116
6.877LeuGlu: 6.877 ± 0.13
2.911LeuPhe: 2.911 ± 0.1
9.38LeuGly: 9.38 ± 0.174
1.269LeuHis: 1.269 ± 0.055
5.352LeuIle: 5.352 ± 0.118
5.192LeuLys: 5.192 ± 0.137
10.949LeuLeu: 10.949 ± 0.204
2.691LeuMet: 2.691 ± 0.082
3.142LeuAsn: 3.142 ± 0.085
4.783LeuPro: 4.783 ± 0.114
2.401LeuGln: 2.401 ± 0.085
8.581LeuArg: 8.581 ± 0.173
9.013LeuSer: 9.013 ± 0.149
5.021LeuThr: 5.021 ± 0.128
8.677LeuVal: 8.677 ± 0.144
1.159LeuTrp: 1.159 ± 0.057
3.977LeuTyr: 3.977 ± 0.099
0.0LeuXaa: 0.0 ± 0.0
Met
2.9MetAla: 2.9 ± 0.095
0.136MetCys: 0.136 ± 0.016
0.968MetAsp: 0.968 ± 0.05
1.371MetGlu: 1.371 ± 0.054
0.777MetPhe: 0.777 ± 0.045
2.181MetGly: 2.181 ± 0.069
0.334MetHis: 0.334 ± 0.027
1.544MetIle: 1.544 ± 0.06
1.222MetLys: 1.222 ± 0.051
2.472MetLeu: 2.472 ± 0.074
0.594MetMet: 0.594 ± 0.036
0.815MetAsn: 0.815 ± 0.047
1.473MetPro: 1.473 ± 0.056
0.494MetGln: 0.494 ± 0.034
1.562MetArg: 1.562 ± 0.065
2.145MetSer: 2.145 ± 0.069
1.266MetThr: 1.266 ± 0.049
1.97MetVal: 1.97 ± 0.06
0.205MetTrp: 0.205 ± 0.021
0.739MetTyr: 0.739 ± 0.04
0.0MetXaa: 0.0 ± 0.0
Asn
2.59AsnAla: 2.59 ± 0.086
0.171AsnCys: 0.171 ± 0.021
1.302AsnAsp: 1.302 ± 0.062
1.627AsnGlu: 1.627 ± 0.06
0.973AsnPhe: 0.973 ± 0.051
2.297AsnGly: 2.297 ± 0.091
0.28AsnHis: 0.28 ± 0.023
1.881AsnIle: 1.881 ± 0.072
0.944AsnLys: 0.944 ± 0.053
2.933AsnLeu: 2.933 ± 0.078
0.846AsnMet: 0.846 ± 0.042
0.95AsnAsn: 0.95 ± 0.054
1.707AsnPro: 1.707 ± 0.069
0.458AsnGln: 0.458 ± 0.035
1.507AsnArg: 1.507 ± 0.052
1.921AsnSer: 1.921 ± 0.07
1.2AsnThr: 1.2 ± 0.061
3.12AsnVal: 3.12 ± 0.116
0.329AsnTrp: 0.329 ± 0.029
1.349AsnTyr: 1.349 ± 0.065
0.0AsnXaa: 0.0 ± 0.0
Pro
3.752ProAla: 3.752 ± 0.1
0.329ProCys: 0.329 ± 0.029
2.332ProAsp: 2.332 ± 0.082
3.71ProGlu: 3.71 ± 0.089
1.78ProPhe: 1.78 ± 0.073
4.106ProGly: 4.106 ± 0.092
0.757ProHis: 0.757 ± 0.045
2.339ProIle: 2.339 ± 0.072
1.914ProLys: 1.914 ± 0.069
5.343ProLeu: 5.343 ± 0.116
1.215ProMet: 1.215 ± 0.05
1.306ProAsn: 1.306 ± 0.069
2.86ProPro: 2.86 ± 0.096
1.569ProGln: 1.569 ± 0.071
3.191ProArg: 3.191 ± 0.089
3.875ProSer: 3.875 ± 0.1
2.306ProThr: 2.306 ± 0.068
3.968ProVal: 3.968 ± 0.096
0.875ProTrp: 0.875 ± 0.045
2.123ProTyr: 2.123 ± 0.07
0.0ProXaa: 0.0 ± 0.0
Gln
2.481GlnAla: 2.481 ± 0.081
0.125GlnCys: 0.125 ± 0.015
0.904GlnAsp: 0.904 ± 0.054
1.578GlnGlu: 1.578 ± 0.063
0.657GlnPhe: 0.657 ± 0.039
2.052GlnGly: 2.052 ± 0.071
0.289GlnHis: 0.289 ± 0.029
0.815GlnIle: 0.815 ± 0.041
0.714GlnLys: 0.714 ± 0.038
3.058GlnLeu: 3.058 ± 0.082
0.556GlnMet: 0.556 ± 0.039
0.487GlnAsn: 0.487 ± 0.035
1.05GlnPro: 1.05 ± 0.051
0.819GlnGln: 0.819 ± 0.057
1.46GlnArg: 1.46 ± 0.055
1.213GlnSer: 1.213 ± 0.045
0.737GlnThr: 0.737 ± 0.038
2.11GlnVal: 2.11 ± 0.067
0.285GlnTrp: 0.285 ± 0.028
0.819GlnTyr: 0.819 ± 0.043
0.0GlnXaa: 0.0 ± 0.0
Arg
6.449ArgAla: 6.449 ± 0.141
0.588ArgCys: 0.588 ± 0.042
3.202ArgAsp: 3.202 ± 0.087
4.72ArgGlu: 4.72 ± 0.111
1.921ArgPhe: 1.921 ± 0.069
5.733ArgGly: 5.733 ± 0.118
0.888ArgHis: 0.888 ± 0.041
2.793ArgIle: 2.793 ± 0.084
2.648ArgLys: 2.648 ± 0.097
7.7ArgLeu: 7.7 ± 0.164
1.233ArgMet: 1.233 ± 0.05
1.447ArgAsn: 1.447 ± 0.054
3.861ArgPro: 3.861 ± 0.1
1.622ArgGln: 1.622 ± 0.058
5.448ArgArg: 5.448 ± 0.152
4.373ArgSer: 4.373 ± 0.101
2.09ArgThr: 2.09 ± 0.062
5.361ArgVal: 5.361 ± 0.119
0.924ArgTrp: 0.924 ± 0.043
2.17ArgTyr: 2.17 ± 0.063
0.0ArgXaa: 0.0 ± 0.0
Ser
5.697SerAla: 5.697 ± 0.128
0.576SerCys: 0.576 ± 0.04
3.013SerAsp: 3.013 ± 0.081
4.081SerGlu: 4.081 ± 0.09
2.57SerPhe: 2.57 ± 0.077
5.915SerGly: 5.915 ± 0.125
0.957SerHis: 0.957 ± 0.051
3.783SerIle: 3.783 ± 0.095
3.064SerLys: 3.064 ± 0.088
9.16SerLeu: 9.16 ± 0.214
2.094SerMet: 2.094 ± 0.068
1.787SerAsn: 1.787 ± 0.078
3.928SerPro: 3.928 ± 0.094
2.116SerGln: 2.116 ± 0.079
5.243SerArg: 5.243 ± 0.153
6.013SerSer: 6.013 ± 0.157
3.249SerThr: 3.249 ± 0.121
5.539SerVal: 5.539 ± 0.115
1.124SerTrp: 1.124 ± 0.052
2.958SerTyr: 2.958 ± 0.097
0.0SerXaa: 0.0 ± 0.0
Thr
4.237ThrAla: 4.237 ± 0.103
0.28ThrCys: 0.28 ± 0.025
1.562ThrAsp: 1.562 ± 0.07
2.063ThrGlu: 2.063 ± 0.079
1.816ThrPhe: 1.816 ± 0.066
3.877ThrGly: 3.877 ± 0.103
0.619ThrHis: 0.619 ± 0.039
2.662ThrIle: 2.662 ± 0.09
1.711ThrLys: 1.711 ± 0.059
4.696ThrLeu: 4.696 ± 0.1
1.099ThrMet: 1.099 ± 0.052
1.184ThrAsn: 1.184 ± 0.048
2.726ThrPro: 2.726 ± 0.086
0.875ThrGln: 0.875 ± 0.047
2.165ThrArg: 2.165 ± 0.07
3.405ThrSer: 3.405 ± 0.104
3.009ThrThr: 3.009 ± 0.212
4.001ThrVal: 4.001 ± 0.109
0.657ThrTrp: 0.657 ± 0.042
2.056ThrTyr: 2.056 ± 0.083
0.0ThrXaa: 0.0 ± 0.0
Val
8.679ValAla: 8.679 ± 0.162
0.483ValCys: 0.483 ± 0.034
4.983ValAsp: 4.983 ± 0.12
6.004ValGlu: 6.004 ± 0.134
2.548ValPhe: 2.548 ± 0.076
6.567ValGly: 6.567 ± 0.126
1.231ValHis: 1.231 ± 0.058
6.109ValIle: 6.109 ± 0.112
5.194ValLys: 5.194 ± 0.122
8.388ValLeu: 8.388 ± 0.144
2.194ValMet: 2.194 ± 0.073
3.356ValAsn: 3.356 ± 0.101
4.489ValPro: 4.489 ± 0.102
1.7ValGln: 1.7 ± 0.061
5.706ValArg: 5.706 ± 0.126
6.639ValSer: 6.639 ± 0.117
4.989ValThr: 4.989 ± 0.115
8.281ValVal: 8.281 ± 0.16
0.748ValTrp: 0.748 ± 0.037
3.269ValTyr: 3.269 ± 0.105
0.0ValXaa: 0.0 ± 0.0
Trp
1.193TrpAla: 1.193 ± 0.06
0.065TrpCys: 0.065 ± 0.014
0.668TrpAsp: 0.668 ± 0.043
0.643TrpGlu: 0.643 ± 0.037
0.394TrpPhe: 0.394 ± 0.032
0.988TrpGly: 0.988 ± 0.048
0.209TrpHis: 0.209 ± 0.023
0.632TrpIle: 0.632 ± 0.037
0.463TrpLys: 0.463 ± 0.032
1.349TrpLeu: 1.349 ± 0.062
0.287TrpMet: 0.287 ± 0.026
0.349TrpAsn: 0.349 ± 0.039
0.639TrpPro: 0.639 ± 0.035
0.307TrpGln: 0.307 ± 0.028
0.935TrpArg: 0.935 ± 0.043
0.941TrpSer: 0.941 ± 0.054
0.507TrpThr: 0.507 ± 0.036
0.915TrpVal: 0.915 ± 0.055
0.267TrpTrp: 0.267 ± 0.028
0.521TrpTyr: 0.521 ± 0.038
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.403TyrAla: 3.403 ± 0.089
0.251TyrCys: 0.251 ± 0.026
2.061TyrAsp: 2.061 ± 0.067
2.301TyrGlu: 2.301 ± 0.074
1.573TyrPhe: 1.573 ± 0.07
3.18TyrGly: 3.18 ± 0.09
0.592TyrHis: 0.592 ± 0.036
2.419TyrIle: 2.419 ± 0.074
1.24TyrLys: 1.24 ± 0.056
4.137TyrLeu: 4.137 ± 0.102
1.168TyrMet: 1.168 ± 0.054
1.449TyrAsn: 1.449 ± 0.075
1.829TyrPro: 1.829 ± 0.067
0.852TyrGln: 0.852 ± 0.049
2.486TyrArg: 2.486 ± 0.075
2.875TyrSer: 2.875 ± 0.095
1.845TyrThr: 1.845 ± 0.068
3.81TyrVal: 3.81 ± 0.102
0.563TyrTrp: 0.563 ± 0.038
1.892TyrTyr: 1.892 ± 0.08
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1499 proteins (449347 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski