Rubellimicrobium thermophilum DSM 16684

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Rubellimicrobium; Rubellimicrobium thermophilum

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3244 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S9S5L8|S9S5L8_9RHOB Carbohydrate ABC transporter substrate-binding protein CUT1 family OS=Rubellimicrobium thermophilum DSM 16684 OX=1123069 GN=ruthe_01740 PE=3 SV=1
MM1 pKa = 8.0DD2 pKa = 5.47RR3 pKa = 11.84LDD5 pKa = 3.66PARR8 pKa = 11.84AYY10 pKa = 9.21AACVLGGTDD19 pKa = 3.76PAAVAAPLEE28 pKa = 4.34DD29 pKa = 4.15LGLTRR34 pKa = 11.84TEE36 pKa = 4.57TEE38 pKa = 3.9DD39 pKa = 3.55GMIVLSADD47 pKa = 3.39GLPYY51 pKa = 10.52AITVDD56 pKa = 3.92PGEE59 pKa = 4.21GHH61 pKa = 7.11CDD63 pKa = 3.14VTSEE67 pKa = 4.83SFGTDD72 pKa = 2.67VAMGKK77 pKa = 10.46LMIVGGMAGFQADD90 pKa = 4.16DD91 pKa = 3.85SAPCIALLIAGIRR104 pKa = 11.84AEE106 pKa = 4.34VTSSGNDD113 pKa = 3.76PICYY117 pKa = 10.13DD118 pKa = 3.51EE119 pKa = 4.43ATSNVRR125 pKa = 11.84FTLADD130 pKa = 3.48GTT132 pKa = 4.18

Molecular weight: 13.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S9SDP8|S9SDP8_9RHOB CRISPR-associated protein Cse3 family OS=Rubellimicrobium thermophilum DSM 16684 OX=1123069 GN=ruthe_02280 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84VRR12 pKa = 11.84KK13 pKa = 9.2ARR15 pKa = 11.84HH16 pKa = 4.71GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.11GGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.91VLSAA44 pKa = 4.11

Molecular weight: 5.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3244

0

3244

933408

22

1723

287.7

31.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.192 ± 0.073

0.897 ± 0.014

5.413 ± 0.032

5.932 ± 0.043

3.261 ± 0.028

9.375 ± 0.044

2.114 ± 0.021

4.534 ± 0.029

1.675 ± 0.033

10.84 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.454 ± 0.019

1.823 ± 0.022

6.282 ± 0.044

2.756 ± 0.021

8.961 ± 0.05

4.343 ± 0.029

4.833 ± 0.028

6.94 ± 0.035

1.615 ± 0.022

1.761 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski