Amino acid dipepetide frequency for Rubellimicrobium thermophilum DSM 16684

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	22.94AlaAla: 22.94 ± 0.241	1.351AlaCys: 1.351 ± 0.044	7.022AlaAsp: 7.022 ± 0.094	9.48AlaGlu: 9.48 ± 0.123	4.515AlaPhe: 4.515 ± 0.082	13.108AlaGly: 13.108 ± 0.136	2.612AlaHis: 2.612 ± 0.053	6.147AlaIle: 6.147 ± 0.09	1.942AlaLys: 1.942 ± 0.053	16.585AlaLeu: 16.585 ± 0.183	4.027AlaMet: 4.027 ± 0.071	2.114AlaAsn: 2.114 ± 0.051	7.235AlaPro: 7.235 ± 0.099	4.262AlaGln: 4.262 ± 0.086	13.285AlaArg: 13.285 ± 0.127	5.328AlaSer: 5.328 ± 0.093	5.927AlaThr: 5.927 ± 0.095	9.751AlaVal: 9.751 ± 0.109	2.044AlaTrp: 2.044 ± 0.053	2.249AlaTyr: 2.249 ± 0.053	0.0AlaXaa: 0.0 ± 0.0
Cys	1.193CysAla: 1.193 ± 0.04	0.148CysCys: 0.148 ± 0.014	0.592CysAsp: 0.592 ± 0.026	0.464CysGlu: 0.464 ± 0.021	0.276CysPhe: 0.276 ± 0.016	0.98CysGly: 0.98 ± 0.038	0.273CysHis: 0.273 ± 0.019	0.356CysIle: 0.356 ± 0.019	0.097CysLys: 0.097 ± 0.01	0.925CysLeu: 0.925 ± 0.031	0.161CysMet: 0.161 ± 0.014	0.176CysAsn: 0.176 ± 0.014	0.675CysPro: 0.675 ± 0.033	0.171CysGln: 0.171 ± 0.014	0.879CysArg: 0.879 ± 0.037	0.4CysSer: 0.4 ± 0.023	0.389CysThr: 0.389 ± 0.02	0.497CysVal: 0.497 ± 0.024	0.162CysTrp: 0.162 ± 0.012	0.159CysTyr: 0.159 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	6.709AspAla: 6.709 ± 0.09	0.468AspCys: 0.468 ± 0.024	2.472AspAsp: 2.472 ± 0.061	2.839AspGlu: 2.839 ± 0.057	1.886AspPhe: 1.886 ± 0.041	5.366AspGly: 5.366 ± 0.09	1.32AspHis: 1.32 ± 0.041	2.333AspIle: 2.333 ± 0.049	0.711AspLys: 0.711 ± 0.028	6.722AspLeu: 6.722 ± 0.102	1.169AspMet: 1.169 ± 0.034	0.871AspAsn: 0.871 ± 0.033	4.756AspPro: 4.756 ± 0.071	1.367AspGln: 1.367 ± 0.039	5.597AspArg: 5.597 ± 0.079	1.941AspSer: 1.941 ± 0.044	1.839AspThr: 1.839 ± 0.046	3.298AspVal: 3.298 ± 0.065	1.292AspTrp: 1.292 ± 0.036	1.18AspTyr: 1.18 ± 0.037	0.0AspXaa: 0.0 ± 0.0
Glu	10.56GluAla: 10.56 ± 0.144	0.311GluCys: 0.311 ± 0.018	3.174GluAsp: 3.174 ± 0.065	4.081GluGlu: 4.081 ± 0.081	1.428GluPhe: 1.428 ± 0.05	6.379GluGly: 6.379 ± 0.093	0.991GluHis: 0.991 ± 0.035	3.222GluIle: 3.222 ± 0.054	1.216GluLys: 1.216 ± 0.044	4.089GluLeu: 4.089 ± 0.074	1.564GluMet: 1.564 ± 0.039	1.064GluAsn: 1.064 ± 0.036	3.02GluPro: 3.02 ± 0.065	1.754GluGln: 1.754 ± 0.046	5.516GluArg: 5.516 ± 0.095	2.107GluSer: 2.107 ± 0.048	3.407GluThr: 3.407 ± 0.063	3.976GluVal: 3.976 ± 0.066	0.738GluTrp: 0.738 ± 0.029	0.725GluTyr: 0.725 ± 0.029	0.0GluXaa: 0.0 ± 0.0
Phe	4.571PheAla: 4.571 ± 0.078	0.378PheCys: 0.378 ± 0.02	2.42PheAsp: 2.42 ± 0.05	1.846PheGlu: 1.846 ± 0.047	1.051PhePhe: 1.051 ± 0.037	3.438PheGly: 3.438 ± 0.075	0.693PheHis: 0.693 ± 0.026	1.16PheIle: 1.16 ± 0.039	0.406PheLys: 0.406 ± 0.023	3.453PheLeu: 3.453 ± 0.067	0.625PheMet: 0.625 ± 0.026	0.693PheAsn: 0.693 ± 0.029	1.61PhePro: 1.61 ± 0.038	0.814PheGln: 0.814 ± 0.029	2.66PheArg: 2.66 ± 0.06	1.383PheSer: 1.383 ± 0.042	1.611PheThr: 1.611 ± 0.046	2.554PheVal: 2.554 ± 0.056	0.551PheTrp: 0.551 ± 0.026	0.687PheTyr: 0.687 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	11.087GlyAla: 11.087 ± 0.124	0.953GlyCys: 0.953 ± 0.028	4.418GlyAsp: 4.418 ± 0.068	5.411GlyGlu: 5.411 ± 0.085	3.43GlyPhe: 3.43 ± 0.059	8.724GlyGly: 8.724 ± 0.137	2.214GlyHis: 2.214 ± 0.057	4.582GlyIle: 4.582 ± 0.07	1.815GlyLys: 1.815 ± 0.051	10.776GlyLeu: 10.776 ± 0.129	2.571GlyMet: 2.571 ± 0.057	1.691GlyAsn: 1.691 ± 0.054	5.131GlyPro: 5.131 ± 0.085	3.155GlyGln: 3.155 ± 0.064	9.013GlyArg: 9.013 ± 0.108	4.201GlySer: 4.201 ± 0.067	4.958GlyThr: 4.958 ± 0.085	5.961GlyVal: 5.961 ± 0.079	1.792GlyTrp: 1.792 ± 0.051	1.866GlyTyr: 1.866 ± 0.051	0.0GlyXaa: 0.0 ± 0.0
His	2.842HisAla: 2.842 ± 0.056	0.196HisCys: 0.196 ± 0.016	1.368HisAsp: 1.368 ± 0.041	1.061HisGlu: 1.061 ± 0.034	0.733HisPhe: 0.733 ± 0.028	2.126HisGly: 2.126 ± 0.057	0.601HisHis: 0.601 ± 0.03	0.643HisIle: 0.643 ± 0.026	0.28HisLys: 0.28 ± 0.016	2.422HisLeu: 2.422 ± 0.056	0.412HisMet: 0.412 ± 0.021	0.336HisAsn: 0.336 ± 0.018	1.782HisPro: 1.782 ± 0.049	0.529HisGln: 0.529 ± 0.025	1.868HisArg: 1.868 ± 0.05	0.737HisSer: 0.737 ± 0.032	0.612HisThr: 0.612 ± 0.027	1.755HisVal: 1.755 ± 0.044	0.375HisTrp: 0.375 ± 0.023	0.459HisTyr: 0.459 ± 0.021	0.0HisXaa: 0.0 ± 0.0
Ile	7.068IleAla: 7.068 ± 0.096	0.455IleCys: 0.455 ± 0.023	2.888IleAsp: 2.888 ± 0.063	3.448IleGlu: 3.448 ± 0.067	1.237IlePhe: 1.237 ± 0.039	4.5IleGly: 4.5 ± 0.087	0.877IleHis: 0.877 ± 0.036	1.523IleIle: 1.523 ± 0.051	0.635IleLys: 0.635 ± 0.027	4.923IleLeu: 4.923 ± 0.08	0.809IleMet: 0.809 ± 0.029	0.892IleAsn: 0.892 ± 0.032	2.462IlePro: 2.462 ± 0.043	0.912IleGln: 0.912 ± 0.028	4.025IleArg: 4.025 ± 0.065	1.709IleSer: 1.709 ± 0.042	2.083IleThr: 2.083 ± 0.051	3.379IleVal: 3.379 ± 0.068	0.692IleTrp: 0.692 ± 0.03	0.822IleTyr: 0.822 ± 0.036	0.0IleXaa: 0.0 ± 0.0
Lys	2.572LysAla: 2.572 ± 0.062	0.081LysCys: 0.081 ± 0.009	0.979LysAsp: 0.979 ± 0.032	0.856LysGlu: 0.856 ± 0.039	0.449LysPhe: 0.449 ± 0.021	1.78LysGly: 1.78 ± 0.051	0.31LysHis: 0.31 ± 0.019	0.767LysIle: 0.767 ± 0.036	0.454LysLys: 0.454 ± 0.029	1.522LysLeu: 1.522 ± 0.044	0.351LysMet: 0.351 ± 0.023	0.298LysAsn: 0.298 ± 0.02	1.055LysPro: 1.055 ± 0.034	0.392LysGln: 0.392 ± 0.024	1.309LysArg: 1.309 ± 0.045	0.77LysSer: 0.77 ± 0.032	0.946LysThr: 0.946 ± 0.033	1.353LysVal: 1.353 ± 0.044	0.207LysTrp: 0.207 ± 0.014	0.3LysTyr: 0.3 ± 0.019	0.0LysXaa: 0.0 ± 0.0
Leu	16.516LeuAla: 16.516 ± 0.165	1.12LeuCys: 1.12 ± 0.036	5.974LeuAsp: 5.974 ± 0.079	5.238LeuGlu: 5.238 ± 0.079	3.403LeuPhe: 3.403 ± 0.073	9.162LeuGly: 9.162 ± 0.119	2.247LeuHis: 2.247 ± 0.041	4.767LeuIle: 4.767 ± 0.083	1.853LeuLys: 1.853 ± 0.056	11.048LeuLeu: 11.048 ± 0.175	2.448LeuMet: 2.448 ± 0.052	1.901LeuAsn: 1.901 ± 0.044	6.982LeuPro: 6.982 ± 0.099	2.408LeuGln: 2.408 ± 0.051	10.291LeuArg: 10.291 ± 0.123	6.331LeuSer: 6.331 ± 0.086	5.706LeuThr: 5.706 ± 0.072	7.278LeuVal: 7.278 ± 0.112	1.798LeuTrp: 1.798 ± 0.056	1.926LeuTyr: 1.926 ± 0.052	0.0LeuXaa: 0.0 ± 0.0
Met	3.725MetAla: 3.725 ± 0.066	0.109MetCys: 0.109 ± 0.012	1.171MetAsp: 1.171 ± 0.034	1.223MetGlu: 1.223 ± 0.04	0.535MetPhe: 0.535 ± 0.025	2.22MetGly: 2.22 ± 0.052	0.395MetHis: 0.395 ± 0.02	1.295MetIle: 1.295 ± 0.036	0.596MetLys: 0.596 ± 0.028	2.363MetLeu: 2.363 ± 0.058	0.558MetMet: 0.558 ± 0.028	0.6MetAsn: 0.6 ± 0.027	1.69MetPro: 1.69 ± 0.044	0.742MetGln: 0.742 ± 0.032	2.139MetArg: 2.139 ± 0.044	1.142MetSer: 1.142 ± 0.038	2.04MetThr: 2.04 ± 0.047	1.563MetVal: 1.563 ± 0.041	0.217MetTrp: 0.217 ± 0.015	0.212MetTyr: 0.212 ± 0.016	0.0MetXaa: 0.0 ± 0.0
Asn	2.372AsnAla: 2.372 ± 0.049	0.193AsnCys: 0.193 ± 0.013	0.928AsnAsp: 0.928 ± 0.034	0.78AsnGlu: 0.78 ± 0.03	0.64AsnPhe: 0.64 ± 0.025	1.65AsnGly: 1.65 ± 0.042	0.364AsnHis: 0.364 ± 0.019	0.87AsnIle: 0.87 ± 0.031	0.288AsnLys: 0.288 ± 0.019	2.053AsnLeu: 2.053 ± 0.048	0.451AsnMet: 0.451 ± 0.023	0.441AsnAsn: 0.441 ± 0.022	1.583AsnPro: 1.583 ± 0.041	0.463AsnGln: 0.463 ± 0.021	1.579AsnArg: 1.579 ± 0.044	0.719AsnSer: 0.719 ± 0.029	0.869AsnThr: 0.869 ± 0.03	1.201AsnVal: 1.201 ± 0.038	0.344AsnTrp: 0.344 ± 0.019	0.445AsnTyr: 0.445 ± 0.022	0.0AsnXaa: 0.0 ± 0.0
Pro	8.915ProAla: 8.915 ± 0.131	0.573ProCys: 0.573 ± 0.028	4.421ProAsp: 4.421 ± 0.077	4.672ProGlu: 4.672 ± 0.073	2.152ProPhe: 2.152 ± 0.049	6.266ProGly: 6.266 ± 0.089	1.377ProHis: 1.377 ± 0.043	1.998ProIle: 1.998 ± 0.049	0.903ProLys: 0.903 ± 0.034	6.166ProLeu: 6.166 ± 0.087	1.392ProMet: 1.392 ± 0.039	0.952ProAsn: 0.952 ± 0.031	4.434ProPro: 4.434 ± 0.09	1.712ProGln: 1.712 ± 0.044	4.72ProArg: 4.72 ± 0.084	2.825ProSer: 2.825 ± 0.057	2.255ProThr: 2.255 ± 0.047	5.019ProVal: 5.019 ± 0.079	0.992ProTrp: 0.992 ± 0.032	1.078ProTyr: 1.078 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	4.294GlnAla: 4.294 ± 0.081	0.157GlnCys: 0.157 ± 0.013	1.474GlnAsp: 1.474 ± 0.042	1.548GlnGlu: 1.548 ± 0.042	0.797GlnPhe: 0.797 ± 0.03	2.888GlnGly: 2.888 ± 0.062	0.475GlnHis: 0.475 ± 0.021	1.576GlnIle: 1.576 ± 0.045	0.609GlnLys: 0.609 ± 0.025	2.152GlnLeu: 2.152 ± 0.044	0.791GlnMet: 0.791 ± 0.03	0.511GlnAsn: 0.511 ± 0.025	1.68GlnPro: 1.68 ± 0.045	0.858GlnGln: 0.858 ± 0.031	2.229GlnArg: 2.229 ± 0.046	1.27GlnSer: 1.27 ± 0.034	1.528GlnThr: 1.528 ± 0.041	1.931GlnVal: 1.931 ± 0.047	0.372GlnTrp: 0.372 ± 0.018	0.424GlnTyr: 0.424 ± 0.022	0.0GlnXaa: 0.0 ± 0.0
Arg	11.52ArgAla: 11.52 ± 0.125	0.696ArgCys: 0.696 ± 0.029	4.684ArgAsp: 4.684 ± 0.081	5.281ArgGlu: 5.281 ± 0.078	2.982ArgPhe: 2.982 ± 0.057	6.778ArgGly: 6.778 ± 0.088	2.323ArgHis: 2.323 ± 0.059	5.019ArgIle: 5.019 ± 0.076	1.588ArgLys: 1.588 ± 0.045	11.152ArgLeu: 11.152 ± 0.119	2.471ArgMet: 2.471 ± 0.052	1.669ArgAsn: 1.669 ± 0.046	6.017ArgPro: 6.017 ± 0.095	2.755ArgGln: 2.755 ± 0.054	8.853ArgArg: 8.853 ± 0.137	3.99ArgSer: 3.99 ± 0.072	3.601ArgThr: 3.601 ± 0.054	5.283ArgVal: 5.283 ± 0.07	1.446ArgTrp: 1.446 ± 0.043	1.5ArgTyr: 1.5 ± 0.037	0.0ArgXaa: 0.0 ± 0.0
Ser	5.381SerAla: 5.381 ± 0.081	0.487SerCys: 0.487 ± 0.026	2.171SerAsp: 2.171 ± 0.047	2.13SerGlu: 2.13 ± 0.044	1.781SerPhe: 1.781 ± 0.048	4.802SerGly: 4.802 ± 0.074	0.887SerHis: 0.887 ± 0.029	1.78SerIle: 1.78 ± 0.052	0.689SerLys: 0.689 ± 0.026	4.696SerLeu: 4.696 ± 0.075	1.056SerMet: 1.056 ± 0.037	0.822SerAsn: 0.822 ± 0.025	3.166SerPro: 3.166 ± 0.058	1.135SerGln: 1.135 ± 0.037	3.789SerArg: 3.789 ± 0.067	2.019SerSer: 2.019 ± 0.053	1.902SerThr: 1.902 ± 0.051	3.097SerVal: 3.097 ± 0.061	0.751SerTrp: 0.751 ± 0.027	0.889SerTyr: 0.889 ± 0.038	0.0SerXaa: 0.0 ± 0.0
Thr	6.357ThrAla: 6.357 ± 0.09	0.384ThrCys: 0.384 ± 0.023	2.589ThrAsp: 2.589 ± 0.055	2.574ThrGlu: 2.574 ± 0.054	1.633ThrPhe: 1.633 ± 0.042	5.514ThrGly: 5.514 ± 0.081	1.005ThrHis: 1.005 ± 0.031	2.222ThrIle: 2.222 ± 0.05	0.75ThrLys: 0.75 ± 0.031	5.66ThrLeu: 5.66 ± 0.084	1.033ThrMet: 1.033 ± 0.036	0.839ThrAsn: 0.839 ± 0.031	3.241ThrPro: 3.241 ± 0.064	1.16ThrGln: 1.16 ± 0.034	3.598ThrArg: 3.598 ± 0.074	1.846ThrSer: 1.846 ± 0.05	2.357ThrThr: 2.357 ± 0.061	4.014ThrVal: 4.014 ± 0.072	0.68ThrTrp: 0.68 ± 0.029	0.87ThrTyr: 0.87 ± 0.032	0.0ThrXaa: 0.0 ± 0.0
Val	9.384ValAla: 9.384 ± 0.117	0.586ValCys: 0.586 ± 0.026	3.352ValAsp: 3.352 ± 0.053	4.455ValGlu: 4.455 ± 0.075	2.453ValPhe: 2.453 ± 0.065	4.896ValGly: 4.896 ± 0.078	1.367ValHis: 1.367 ± 0.041	3.65ValIle: 3.65 ± 0.069	1.283ValLys: 1.283 ± 0.045	7.956ValLeu: 7.956 ± 0.102	1.896ValMet: 1.896 ± 0.047	1.575ValAsn: 1.575 ± 0.044	4.318ValPro: 4.318 ± 0.071	1.866ValGln: 1.866 ± 0.046	5.052ValArg: 5.052 ± 0.075	3.174ValSer: 3.174 ± 0.068	4.601ValThr: 4.601 ± 0.078	5.294ValVal: 5.294 ± 0.09	1.081ValTrp: 1.081 ± 0.036	1.158ValTyr: 1.158 ± 0.037	0.0ValXaa: 0.0 ± 0.0
Trp	1.747TrpAla: 1.747 ± 0.047	0.148TrpCys: 0.148 ± 0.013	0.834TrpAsp: 0.834 ± 0.037	0.901TrpGlu: 0.901 ± 0.032	0.583TrpPhe: 0.583 ± 0.025	1.301TrpGly: 1.301 ± 0.039	0.403TrpHis: 0.403 ± 0.02	0.812TrpIle: 0.812 ± 0.034	0.331TrpLys: 0.331 ± 0.022	1.863TrpLeu: 1.863 ± 0.054	0.412TrpMet: 0.412 ± 0.021	0.382TrpAsn: 0.382 ± 0.019	1.016TrpPro: 1.016 ± 0.037	0.658TrpGln: 0.658 ± 0.026	1.565TrpArg: 1.565 ± 0.041	0.834TrpSer: 0.834 ± 0.032	0.93TrpThr: 0.93 ± 0.035	0.876TrpVal: 0.876 ± 0.034	0.307TrpTrp: 0.307 ± 0.019	0.242TrpTyr: 0.242 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.168TyrAla: 2.168 ± 0.054	0.177TyrCys: 0.177 ± 0.012	1.203TyrAsp: 1.203 ± 0.04	1.035TyrGlu: 1.035 ± 0.031	0.643TyrPhe: 0.643 ± 0.026	1.874TyrGly: 1.874 ± 0.05	0.403TyrHis: 0.403 ± 0.022	0.616TyrIle: 0.616 ± 0.027	0.305TyrLys: 0.305 ± 0.017	1.871TyrLeu: 1.871 ± 0.047	0.34TyrMet: 0.34 ± 0.017	0.405TyrAsn: 0.405 ± 0.021	0.973TyrPro: 0.973 ± 0.033	0.449TyrGln: 0.449 ± 0.021	1.637TyrArg: 1.637 ± 0.041	0.704TyrSer: 0.704 ± 0.027	0.766TyrThr: 0.766 ± 0.029	1.319TyrVal: 1.319 ± 0.038	0.303TyrTrp: 0.303 ± 0.019	0.416TyrTyr: 0.416 ± 0.021	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3244 proteins (933408 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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