Halomonas ilicicola DSM 19980
Average proteome isoelectric point is 6.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3660 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M5BC90|A0A1M5BC90_9GAMM Ubiquinone biosynthesis protein OS=Halomonas ilicicola DSM 19980 OX=1121942 GN=SAMN02745148_02510 PE=4 SV=1
MM1 pKa = 7.32 RR2 pKa = 11.84 TLHH5 pKa = 5.84 TFMLAGALTALALPAAAASGHH26 pKa = 5.15 WSVGAEE32 pKa = 4.01 VGTTGYY38 pKa = 10.52 GGSLSYY44 pKa = 10.56 RR45 pKa = 11.84 FHH47 pKa = 8.08 DD48 pKa = 4.19 NLALTAGYY56 pKa = 10.58 SGFTYY61 pKa = 10.89 DD62 pKa = 4.94 DD63 pKa = 3.63 IEE65 pKa = 4.83 YY66 pKa = 8.19 EE67 pKa = 4.18 TDD69 pKa = 3.32 DD70 pKa = 4.07 ADD72 pKa = 3.87 YY73 pKa = 11.47 DD74 pKa = 4.18 GDD76 pKa = 3.52 VDD78 pKa = 3.66 MDD80 pKa = 4.26 VYY82 pKa = 11.41 GLTLDD87 pKa = 3.64 YY88 pKa = 10.93 FPFGGRR94 pKa = 11.84 FFLSAGAVKK103 pKa = 10.07 PDD105 pKa = 3.1 IAALVIGRR113 pKa = 11.84 PKK115 pKa = 10.64 DD116 pKa = 3.7 GQSYY120 pKa = 9.34 EE121 pKa = 4.38 YY122 pKa = 11.04 NGNTYY127 pKa = 7.95 TAEE130 pKa = 4.04 EE131 pKa = 4.46 VGTLRR136 pKa = 11.84 GDD138 pKa = 3.37 VTLGDD143 pKa = 4.39 SIQPYY148 pKa = 10.42 LGLGWRR154 pKa = 11.84 GSHH157 pKa = 6.73 KK158 pKa = 10.74 SGLGVFAKK166 pKa = 10.63 LGAFLTDD173 pKa = 3.21 AEE175 pKa = 4.78 VNLEE179 pKa = 4.02 ASGAASDD186 pKa = 3.7 PQLRR190 pKa = 11.84 EE191 pKa = 4.71 DD192 pKa = 3.61 IQQEE196 pKa = 4.03 EE197 pKa = 5.37 DD198 pKa = 3.93 DD199 pKa = 4.67 LQDD202 pKa = 4.11 DD203 pKa = 3.68 VDD205 pKa = 3.9 EE206 pKa = 5.23 FGVYY210 pKa = 9.34 PVAVLGLEE218 pKa = 4.23 YY219 pKa = 10.68 TFF221 pKa = 5.29
Molecular weight: 23.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.744
IPC2_protein 3.897
IPC_protein 3.91
Toseland 3.681
ProMoST 4.062
Dawson 3.897
Bjellqvist 4.05
Wikipedia 3.834
Rodwell 3.732
Grimsley 3.592
Solomon 3.884
Lehninger 3.846
Nozaki 4.012
DTASelect 4.253
Thurlkill 3.732
EMBOSS 3.846
Sillero 4.024
Patrickios 0.896
IPC_peptide 3.884
IPC2_peptide 3.999
IPC2.peptide.svr19 3.908
Protein with the highest isoelectric point:
>tr|A0A1M4VVQ6|A0A1M4VVQ6_9GAMM Iron-sulfur cluster insertion protein ErpA OS=Halomonas ilicicola DSM 19980 OX=1121942 GN=erpA PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVLKK11 pKa = 10.34 RR12 pKa = 11.84 KK13 pKa = 9.1 RR14 pKa = 11.84 VHH16 pKa = 6.26 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 NGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.93 GRR39 pKa = 11.84 KK40 pKa = 8.85 RR41 pKa = 11.84 LSAA44 pKa = 3.96
Molecular weight: 5.21 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.257
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.705
Grimsley 13.042
Solomon 13.51
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.427
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.173
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3660
0
3660
1184749
39
5047
323.7
35.61
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.945 ± 0.051
0.918 ± 0.016
5.82 ± 0.039
6.585 ± 0.047
3.506 ± 0.026
8.234 ± 0.045
2.407 ± 0.026
4.819 ± 0.031
2.738 ± 0.029
11.535 ± 0.068
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.46 ± 0.02
2.562 ± 0.028
4.883 ± 0.029
3.804 ± 0.028
7.433 ± 0.053
5.391 ± 0.031
4.97 ± 0.031
7.082 ± 0.033
1.502 ± 0.021
2.409 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here