Streptococcus phage Javan23
Average proteome isoelectric point is 6.21
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 56 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4D6A8W1|A0A4D6A8W1_9CAUD Uncharacterized protein OS=Streptococcus phage Javan23 OX=2548057 GN=Javan23_0013 PE=4 SV=1
MM1 pKa = 7.52 LFGASNEE8 pKa = 4.02 YY9 pKa = 10.49 GKK11 pKa = 8.46 TVGYY15 pKa = 10.51 FDD17 pKa = 5.62 LNLVIDD23 pKa = 4.41 KK24 pKa = 9.89 PQDD27 pKa = 3.26 KK28 pKa = 10.74 LIYY31 pKa = 9.44 CVLMDD36 pKa = 4.09 SANLVKK42 pKa = 10.78 SLDD45 pKa = 3.59 DD46 pKa = 3.72 YY47 pKa = 10.82 YY48 pKa = 11.33 TYY50 pKa = 10.44 LTFANKK56 pKa = 9.18 EE57 pKa = 3.95
Molecular weight: 6.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.138
IPC2_protein 4.457
IPC_protein 4.253
Toseland 4.037
ProMoST 4.304
Dawson 4.253
Bjellqvist 4.533
Wikipedia 4.215
Rodwell 4.075
Grimsley 3.961
Solomon 4.228
Lehninger 4.19
Nozaki 4.38
DTASelect 4.635
Thurlkill 4.113
EMBOSS 4.215
Sillero 4.368
Patrickios 0.782
IPC_peptide 4.228
IPC2_peptide 4.342
IPC2.peptide.svr19 4.38
Protein with the highest isoelectric point:
>tr|A0A4D6A902|A0A4D6A902_9CAUD Uncharacterized protein OS=Streptococcus phage Javan23 OX=2548057 GN=Javan23_0053 PE=4 SV=1
MM1 pKa = 7.13 SQGISVKK8 pKa = 10.26 VDD10 pKa = 2.73 VDD12 pKa = 3.79 LAGATRR18 pKa = 11.84 KK19 pKa = 9.81 VSPASVARR27 pKa = 11.84 GRR29 pKa = 11.84 VAMASQMMMDD39 pKa = 4.02 MDD41 pKa = 4.45 RR42 pKa = 11.84 YY43 pKa = 10.1 IPLRR47 pKa = 11.84 SGGGALRR54 pKa = 11.84 ASASMGGSGKK64 pKa = 8.8 TISYY68 pKa = 6.95 NTVYY72 pKa = 10.89 ARR74 pKa = 11.84 AQFYY78 pKa = 7.61 GTNGIVTFSKK88 pKa = 9.65 YY89 pKa = 5.91 TTPGTGKK96 pKa = 10.34 DD97 pKa = 2.91 WLKK100 pKa = 9.95 PAKK103 pKa = 9.44 EE104 pKa = 4.22 ANLEE108 pKa = 4.02 SWKK111 pKa = 10.66 QKK113 pKa = 10.31 ALKK116 pKa = 10.6 EE117 pKa = 4.03 MGFF120 pKa = 3.66
Molecular weight: 12.85 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.162
IPC2_protein 9.56
IPC_protein 9.633
Toseland 10.248
ProMoST 9.867
Dawson 10.409
Bjellqvist 10.043
Wikipedia 10.57
Rodwell 10.935
Grimsley 10.467
Solomon 10.438
Lehninger 10.409
Nozaki 10.204
DTASelect 10.043
Thurlkill 10.262
EMBOSS 10.628
Sillero 10.306
Patrickios 10.657
IPC_peptide 10.438
IPC2_peptide 8.565
IPC2.peptide.svr19 8.64
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
56
0
56
11642
41
1269
207.9
23.4
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.769 ± 0.947
0.593 ± 0.097
6.614 ± 0.457
6.365 ± 0.605
4.441 ± 0.211
6.477 ± 0.688
1.331 ± 0.152
7.859 ± 0.286
8.255 ± 0.446
7.774 ± 0.25
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.465 ± 0.175
6.124 ± 0.382
2.671 ± 0.248
4.269 ± 0.228
3.616 ± 0.248
6.76 ± 0.456
6.382 ± 0.348
6.219 ± 0.296
1.151 ± 0.133
3.865 ± 0.404
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here