Rhodococcus phage ReqiPepy6

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Pepyhexavirus; Rhodococcus virus Pepy6

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 107 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D4P7B4|D4P7B4_9CAUD Gp003 OS=Rhodococcus phage ReqiPepy6 OX=691965 GN=Pepy6gene003 PE=4 SV=1
MM1 pKa = 8.24DD2 pKa = 6.68DD3 pKa = 4.0YY4 pKa = 11.36MLSTSDD10 pKa = 4.38NPFNPYY16 pKa = 7.55TQWEE20 pKa = 3.85QWYY23 pKa = 9.84AFDD26 pKa = 4.97AAAGYY31 pKa = 7.53HH32 pKa = 5.21TPAYY36 pKa = 9.62LARR39 pKa = 11.84IVVTSHH45 pKa = 6.18EE46 pKa = 4.36LSEE49 pKa = 4.47ADD51 pKa = 3.46QLLAIQQGIDD61 pKa = 3.81EE62 pKa = 4.46ILEE65 pKa = 4.09MNLTGNYY72 pKa = 8.69IKK74 pKa = 11.01VMNPAAA80 pKa = 4.31

Molecular weight: 9.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D4P7B9|D4P7B9_9CAUD Gp008 OS=Rhodococcus phage ReqiPepy6 OX=691965 GN=Pepy6gene008 PE=4 SV=1
MM1 pKa = 7.6EE2 pKa = 5.6LMLGAILLLIAATWPQAPSALRR24 pKa = 11.84FLWGSVQGLFATRR37 pKa = 11.84YY38 pKa = 8.72VVIKK42 pKa = 9.22YY43 pKa = 9.25HH44 pKa = 5.5HH45 pKa = 5.84TADD48 pKa = 3.77RR49 pKa = 11.84FATHH53 pKa = 6.11GGKK56 pKa = 9.9RR57 pKa = 11.84FRR59 pKa = 11.84TMFWAMRR66 pKa = 11.84HH67 pKa = 4.45NRR69 pKa = 11.84RR70 pKa = 11.84MNNLYY75 pKa = 8.77TASRR79 pKa = 11.84VKK81 pKa = 9.92FHH83 pKa = 6.37VPEE86 pKa = 4.63IEE88 pKa = 3.75YY89 pKa = 9.87FYY91 pKa = 10.56TVKK94 pKa = 10.59PLVALTT100 pKa = 3.81

Molecular weight: 11.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

107

0

107

23886

35

1950

223.2

24.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.746 ± 0.345

0.548 ± 0.08

6.192 ± 0.237

6.225 ± 0.253

3.58 ± 0.173

7.682 ± 0.372

2.001 ± 0.156

5.865 ± 0.26

6.175 ± 0.32

8.13 ± 0.27

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.755 ± 0.141

4.647 ± 0.215

4.074 ± 0.217

3.286 ± 0.136

5.087 ± 0.252

6.573 ± 0.183

6.64 ± 0.244

7.008 ± 0.201

1.34 ± 0.098

3.446 ± 0.246
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski