Amino acid dipepetide frequency for Rhodococcus phage ReqiPepy6

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.253AlaAla: 9.253 ± 0.903
0.293AlaCys: 0.293 ± 0.118
4.982AlaAsp: 4.982 ± 0.473
4.605AlaGlu: 4.605 ± 0.551
3.14AlaPhe: 3.14 ± 0.356
6.824AlaGly: 6.824 ± 0.845
2.01AlaHis: 2.01 ± 0.34
5.485AlaIle: 5.485 ± 0.738
4.898AlaLys: 4.898 ± 0.552
7.369AlaLeu: 7.369 ± 0.971
2.428AlaMet: 2.428 ± 0.405
4.061AlaAsn: 4.061 ± 0.416
2.973AlaPro: 2.973 ± 0.426
2.973AlaGln: 2.973 ± 0.371
4.187AlaArg: 4.187 ± 0.609
5.401AlaSer: 5.401 ± 0.553
5.778AlaThr: 5.778 ± 0.463
6.489AlaVal: 6.489 ± 0.485
1.214AlaTrp: 1.214 ± 0.191
3.098AlaTyr: 3.098 ± 0.373
0.0AlaXaa: 0.0 ± 0.0
Cys
0.377CysAla: 0.377 ± 0.154
0.126CysCys: 0.126 ± 0.067
0.293CysAsp: 0.293 ± 0.099
0.335CysGlu: 0.335 ± 0.134
0.167CysPhe: 0.167 ± 0.084
0.419CysGly: 0.419 ± 0.146
0.084CysHis: 0.084 ± 0.056
0.461CysIle: 0.461 ± 0.172
0.167CysLys: 0.167 ± 0.105
0.712CysLeu: 0.712 ± 0.205
0.209CysMet: 0.209 ± 0.09
0.209CysAsn: 0.209 ± 0.111
0.293CysPro: 0.293 ± 0.128
0.084CysGln: 0.084 ± 0.06
0.502CysArg: 0.502 ± 0.179
0.293CysSer: 0.293 ± 0.101
0.293CysThr: 0.293 ± 0.137
0.251CysVal: 0.251 ± 0.121
0.126CysTrp: 0.126 ± 0.066
0.084CysTyr: 0.084 ± 0.059
0.0CysXaa: 0.0 ± 0.0
Asp
5.987AspAla: 5.987 ± 0.584
0.209AspCys: 0.209 ± 0.094
4.354AspAsp: 4.354 ± 0.498
5.024AspGlu: 5.024 ± 0.429
3.14AspPhe: 3.14 ± 0.368
4.438AspGly: 4.438 ± 0.455
0.963AspHis: 0.963 ± 0.224
3.642AspIle: 3.642 ± 0.449
3.098AspLys: 3.098 ± 0.386
5.108AspLeu: 5.108 ± 0.531
1.298AspMet: 1.298 ± 0.209
2.47AspAsn: 2.47 ± 0.388
2.554AspPro: 2.554 ± 0.386
2.135AspGln: 2.135 ± 0.318
3.266AspArg: 3.266 ± 0.349
3.684AspSer: 3.684 ± 0.431
4.354AspThr: 4.354 ± 0.385
3.349AspVal: 3.349 ± 0.356
0.712AspTrp: 0.712 ± 0.146
2.135AspTyr: 2.135 ± 0.279
0.0AspXaa: 0.0 ± 0.0
Glu
5.568GluAla: 5.568 ± 0.406
0.126GluCys: 0.126 ± 0.085
3.852GluAsp: 3.852 ± 0.444
4.438GluGlu: 4.438 ± 0.599
2.47GluPhe: 2.47 ± 0.296
4.187GluGly: 4.187 ± 0.679
1.382GluHis: 1.382 ± 0.26
4.438GluIle: 4.438 ± 0.55
4.354GluLys: 4.354 ± 0.487
5.61GluLeu: 5.61 ± 0.456
1.926GluMet: 1.926 ± 0.309
2.093GluAsn: 2.093 ± 0.303
1.465GluPro: 1.465 ± 0.25
2.596GluGln: 2.596 ± 0.326
3.475GluArg: 3.475 ± 0.477
3.475GluSer: 3.475 ± 0.431
3.391GluThr: 3.391 ± 0.304
4.187GluVal: 4.187 ± 0.392
1.214GluTrp: 1.214 ± 0.206
2.01GluTyr: 2.01 ± 0.307
0.0GluXaa: 0.0 ± 0.0
Phe
1.842PheAla: 1.842 ± 0.203
0.377PheCys: 0.377 ± 0.137
3.601PheAsp: 3.601 ± 0.415
2.177PheGlu: 2.177 ± 0.31
1.047PhePhe: 1.047 ± 0.216
3.852PheGly: 3.852 ± 0.468
0.67PheHis: 0.67 ± 0.19
1.591PheIle: 1.591 ± 0.287
1.842PheLys: 1.842 ± 0.335
3.182PheLeu: 3.182 ± 0.383
0.754PheMet: 0.754 ± 0.18
2.01PheAsn: 2.01 ± 0.295
1.423PhePro: 1.423 ± 0.219
0.879PheGln: 0.879 ± 0.194
1.8PheArg: 1.8 ± 0.257
2.01PheSer: 2.01 ± 0.32
2.177PheThr: 2.177 ± 0.295
2.763PheVal: 2.763 ± 0.406
0.67PheTrp: 0.67 ± 0.151
1.13PheTyr: 1.13 ± 0.263
0.0PheXaa: 0.0 ± 0.0
Gly
6.531GlyAla: 6.531 ± 0.94
0.335GlyCys: 0.335 ± 0.121
4.94GlyAsp: 4.94 ± 0.527
4.27GlyGlu: 4.27 ± 0.382
2.428GlyPhe: 2.428 ± 0.388
6.531GlyGly: 6.531 ± 0.701
0.921GlyHis: 0.921 ± 0.259
4.564GlyIle: 4.564 ± 0.798
3.768GlyLys: 3.768 ± 0.285
6.196GlyLeu: 6.196 ± 1.02
2.973GlyMet: 2.973 ± 0.48
3.224GlyAsn: 3.224 ± 0.507
2.051GlyPro: 2.051 ± 0.317
1.717GlyGln: 1.717 ± 0.217
4.019GlyArg: 4.019 ± 0.457
5.526GlySer: 5.526 ± 0.493
5.861GlyThr: 5.861 ± 0.513
7.369GlyVal: 7.369 ± 0.636
0.879GlyTrp: 0.879 ± 0.22
2.721GlyTyr: 2.721 ± 0.404
0.0GlyXaa: 0.0 ± 0.0
His
1.507HisAla: 1.507 ± 0.328
0.084HisCys: 0.084 ± 0.06
0.837HisAsp: 0.837 ± 0.173
1.13HisGlu: 1.13 ± 0.188
0.754HisPhe: 0.754 ± 0.202
1.423HisGly: 1.423 ± 0.286
0.754HisHis: 0.754 ± 0.196
1.675HisIle: 1.675 ± 0.303
1.13HisLys: 1.13 ± 0.226
1.382HisLeu: 1.382 ± 0.242
0.419HisMet: 0.419 ± 0.159
1.089HisAsn: 1.089 ± 0.235
1.13HisPro: 1.13 ± 0.223
0.502HisGln: 0.502 ± 0.168
1.13HisArg: 1.13 ± 0.201
1.172HisSer: 1.172 ± 0.228
1.34HisThr: 1.34 ± 0.232
1.089HisVal: 1.089 ± 0.242
0.335HisTrp: 0.335 ± 0.122
1.13HisTyr: 1.13 ± 0.212
0.0HisXaa: 0.0 ± 0.0
Ile
4.689IleAla: 4.689 ± 0.454
0.461IleCys: 0.461 ± 0.165
4.48IleAsp: 4.48 ± 0.303
4.857IleGlu: 4.857 ± 0.585
1.8IlePhe: 1.8 ± 0.263
4.438IleGly: 4.438 ± 0.773
1.214IleHis: 1.214 ± 0.229
2.763IleIle: 2.763 ± 0.335
3.894IleLys: 3.894 ± 0.526
4.857IleLeu: 4.857 ± 0.445
1.172IleMet: 1.172 ± 0.244
2.68IleAsn: 2.68 ± 0.281
2.135IlePro: 2.135 ± 0.281
2.051IleGln: 2.051 ± 0.338
2.805IleArg: 2.805 ± 0.324
3.684IleSer: 3.684 ± 0.397
3.726IleThr: 3.726 ± 0.44
4.396IleVal: 4.396 ± 0.478
0.544IleTrp: 0.544 ± 0.137
2.01IleTyr: 2.01 ± 0.309
0.0IleXaa: 0.0 ± 0.0
Lys
5.359LysAla: 5.359 ± 0.601
0.251LysCys: 0.251 ± 0.115
3.391LysAsp: 3.391 ± 0.497
3.601LysGlu: 3.601 ± 0.391
2.135LysPhe: 2.135 ± 0.35
3.977LysGly: 3.977 ± 0.561
1.465LysHis: 1.465 ± 0.29
4.019LysIle: 4.019 ± 0.416
4.564LysLys: 4.564 ± 0.664
5.066LysLeu: 5.066 ± 0.551
1.675LysMet: 1.675 ± 0.263
3.224LysAsn: 3.224 ± 0.371
2.721LysPro: 2.721 ± 0.401
2.805LysGln: 2.805 ± 0.347
2.847LysArg: 2.847 ± 0.375
3.768LysSer: 3.768 ± 0.426
4.019LysThr: 4.019 ± 0.394
3.977LysVal: 3.977 ± 0.386
1.005LysTrp: 1.005 ± 0.19
1.884LysTyr: 1.884 ± 0.328
0.0LysXaa: 0.0 ± 0.0
Leu
7.955LeuAla: 7.955 ± 0.833
0.167LeuCys: 0.167 ± 0.088
5.066LeuAsp: 5.066 ± 0.544
4.145LeuGlu: 4.145 ± 0.383
3.014LeuPhe: 3.014 ± 0.403
5.61LeuGly: 5.61 ± 0.693
1.465LeuHis: 1.465 ± 0.283
5.192LeuIle: 5.192 ± 0.466
6.28LeuLys: 6.28 ± 0.566
5.82LeuLeu: 5.82 ± 0.621
2.261LeuMet: 2.261 ± 0.349
3.977LeuAsn: 3.977 ± 0.349
2.721LeuPro: 2.721 ± 0.288
2.554LeuGln: 2.554 ± 0.291
3.308LeuArg: 3.308 ± 0.303
5.694LeuSer: 5.694 ± 0.429
6.28LeuThr: 6.28 ± 0.549
5.694LeuVal: 5.694 ± 0.57
0.795LeuTrp: 0.795 ± 0.195
3.308LeuTyr: 3.308 ± 0.497
0.0LeuXaa: 0.0 ± 0.0
Met
2.596MetAla: 2.596 ± 0.383
0.209MetCys: 0.209 ± 0.088
1.382MetAsp: 1.382 ± 0.212
1.298MetGlu: 1.298 ± 0.229
0.712MetPhe: 0.712 ± 0.181
1.675MetGly: 1.675 ± 0.256
0.419MetHis: 0.419 ± 0.126
1.717MetIle: 1.717 ± 0.237
1.968MetLys: 1.968 ± 0.311
2.847MetLeu: 2.847 ± 0.283
0.712MetMet: 0.712 ± 0.175
1.298MetAsn: 1.298 ± 0.252
1.423MetPro: 1.423 ± 0.224
0.461MetGln: 0.461 ± 0.139
1.465MetArg: 1.465 ± 0.191
2.638MetSer: 2.638 ± 0.313
2.47MetThr: 2.47 ± 0.289
1.591MetVal: 1.591 ± 0.244
0.293MetTrp: 0.293 ± 0.098
0.377MetTyr: 0.377 ± 0.134
0.0MetXaa: 0.0 ± 0.0
Asn
3.894AsnAla: 3.894 ± 0.337
0.293AsnCys: 0.293 ± 0.111
2.889AsnAsp: 2.889 ± 0.353
2.973AsnGlu: 2.973 ± 0.418
1.675AsnPhe: 1.675 ± 0.314
4.145AsnGly: 4.145 ± 0.394
0.837AsnHis: 0.837 ± 0.194
2.805AsnIle: 2.805 ± 0.389
2.638AsnLys: 2.638 ± 0.298
3.182AsnLeu: 3.182 ± 0.347
1.13AsnMet: 1.13 ± 0.202
2.345AsnAsn: 2.345 ± 0.435
2.805AsnPro: 2.805 ± 0.442
1.507AsnGln: 1.507 ± 0.238
3.014AsnArg: 3.014 ± 0.367
3.182AsnSer: 3.182 ± 0.34
2.261AsnThr: 2.261 ± 0.337
3.14AsnVal: 3.14 ± 0.35
0.377AsnTrp: 0.377 ± 0.188
1.34AsnTyr: 1.34 ± 0.234
0.0AsnXaa: 0.0 ± 0.0
Pro
3.601ProAla: 3.601 ± 0.424
0.419ProCys: 0.419 ± 0.142
2.554ProAsp: 2.554 ± 0.39
3.056ProGlu: 3.056 ± 0.46
1.298ProPhe: 1.298 ± 0.221
2.973ProGly: 2.973 ± 0.386
0.712ProHis: 0.712 ± 0.199
1.884ProIle: 1.884 ± 0.288
2.386ProLys: 2.386 ± 0.279
2.721ProLeu: 2.721 ± 0.287
1.465ProMet: 1.465 ± 0.284
1.884ProAsn: 1.884 ± 0.382
2.093ProPro: 2.093 ± 0.377
0.879ProGln: 0.879 ± 0.2
2.219ProArg: 2.219 ± 0.266
2.889ProSer: 2.889 ± 0.401
2.805ProThr: 2.805 ± 0.432
2.68ProVal: 2.68 ± 0.365
0.67ProTrp: 0.67 ± 0.171
1.549ProTyr: 1.549 ± 0.282
0.0ProXaa: 0.0 ± 0.0
Gln
3.601GlnAla: 3.601 ± 0.475
0.126GlnCys: 0.126 ± 0.064
1.423GlnAsp: 1.423 ± 0.229
1.968GlnGlu: 1.968 ± 0.297
1.34GlnPhe: 1.34 ± 0.244
2.261GlnGly: 2.261 ± 0.279
0.795GlnHis: 0.795 ± 0.223
1.675GlnIle: 1.675 ± 0.326
2.345GlnLys: 2.345 ± 0.33
3.433GlnLeu: 3.433 ± 0.402
0.795GlnMet: 0.795 ± 0.164
1.256GlnAsn: 1.256 ± 0.208
1.214GlnPro: 1.214 ± 0.193
1.549GlnGln: 1.549 ± 0.236
1.591GlnArg: 1.591 ± 0.256
1.926GlnSer: 1.926 ± 0.294
2.01GlnThr: 2.01 ± 0.335
1.8GlnVal: 1.8 ± 0.254
0.628GlnTrp: 0.628 ± 0.196
1.13GlnTyr: 1.13 ± 0.184
0.0GlnXaa: 0.0 ± 0.0
Arg
3.852ArgAla: 3.852 ± 0.408
0.377ArgCys: 0.377 ± 0.126
2.931ArgAsp: 2.931 ± 0.315
3.768ArgGlu: 3.768 ± 0.439
1.675ArgPhe: 1.675 ± 0.253
2.554ArgGly: 2.554 ± 0.333
1.507ArgHis: 1.507 ± 0.279
2.805ArgIle: 2.805 ± 0.36
3.433ArgLys: 3.433 ± 0.613
3.684ArgLeu: 3.684 ± 0.446
1.465ArgMet: 1.465 ± 0.22
2.386ArgAsn: 2.386 ± 0.356
2.721ArgPro: 2.721 ± 0.337
2.219ArgGln: 2.219 ± 0.334
3.601ArgArg: 3.601 ± 0.494
3.14ArgSer: 3.14 ± 0.353
2.847ArgThr: 2.847 ± 0.291
3.517ArgVal: 3.517 ± 0.435
0.544ArgTrp: 0.544 ± 0.148
1.842ArgTyr: 1.842 ± 0.269
0.0ArgXaa: 0.0 ± 0.0
Ser
4.773SerAla: 4.773 ± 0.451
0.67SerCys: 0.67 ± 0.188
4.061SerAsp: 4.061 ± 0.551
3.936SerGlu: 3.936 ± 0.373
1.842SerPhe: 1.842 ± 0.296
6.866SerGly: 6.866 ± 0.572
1.298SerHis: 1.298 ± 0.225
3.433SerIle: 3.433 ± 0.423
3.768SerLys: 3.768 ± 0.449
4.982SerLeu: 4.982 ± 0.429
2.219SerMet: 2.219 ± 0.259
2.638SerAsn: 2.638 ± 0.247
2.512SerPro: 2.512 ± 0.312
2.219SerGln: 2.219 ± 0.319
3.224SerArg: 3.224 ± 0.403
4.815SerSer: 4.815 ± 0.574
4.982SerThr: 4.982 ± 0.549
4.103SerVal: 4.103 ± 0.42
1.047SerTrp: 1.047 ± 0.207
2.345SerTyr: 2.345 ± 0.362
0.0SerXaa: 0.0 ± 0.0
Thr
5.694ThrAla: 5.694 ± 0.591
0.293ThrCys: 0.293 ± 0.107
3.308ThrAsp: 3.308 ± 0.317
3.517ThrGlu: 3.517 ± 0.387
2.68ThrPhe: 2.68 ± 0.311
4.396ThrGly: 4.396 ± 0.46
1.34ThrHis: 1.34 ± 0.236
4.27ThrIle: 4.27 ± 0.422
4.438ThrLys: 4.438 ± 0.298
5.945ThrLeu: 5.945 ± 0.525
1.172ThrMet: 1.172 ± 0.212
3.433ThrAsn: 3.433 ± 0.442
3.81ThrPro: 3.81 ± 0.484
2.093ThrGln: 2.093 ± 0.297
2.763ThrArg: 2.763 ± 0.372
4.27ThrSer: 4.27 ± 0.473
4.564ThrThr: 4.564 ± 0.559
4.982ThrVal: 4.982 ± 0.433
0.963ThrTrp: 0.963 ± 0.225
2.47ThrTyr: 2.47 ± 0.369
0.0ThrXaa: 0.0 ± 0.0
Val
6.448ValAla: 6.448 ± 0.477
0.251ValCys: 0.251 ± 0.112
4.48ValAsp: 4.48 ± 0.385
4.145ValGlu: 4.145 ± 0.404
2.01ValPhe: 2.01 ± 0.256
6.154ValGly: 6.154 ± 0.595
1.214ValHis: 1.214 ± 0.238
3.308ValIle: 3.308 ± 0.347
4.312ValLys: 4.312 ± 0.397
5.652ValLeu: 5.652 ± 0.484
2.177ValMet: 2.177 ± 0.291
3.308ValAsn: 3.308 ± 0.373
2.763ValPro: 2.763 ± 0.328
2.177ValGln: 2.177 ± 0.304
3.433ValArg: 3.433 ± 0.419
5.233ValSer: 5.233 ± 0.487
4.187ValThr: 4.187 ± 0.485
5.568ValVal: 5.568 ± 0.541
0.837ValTrp: 0.837 ± 0.21
2.428ValTyr: 2.428 ± 0.452
0.0ValXaa: 0.0 ± 0.0
Trp
0.879TrpAla: 0.879 ± 0.175
0.126TrpCys: 0.126 ± 0.073
0.963TrpAsp: 0.963 ± 0.207
1.005TrpGlu: 1.005 ± 0.187
0.502TrpPhe: 0.502 ± 0.127
1.13TrpGly: 1.13 ± 0.182
0.377TrpHis: 0.377 ± 0.115
0.67TrpIle: 0.67 ± 0.171
0.586TrpLys: 0.586 ± 0.149
0.795TrpLeu: 0.795 ± 0.179
0.502TrpMet: 0.502 ± 0.185
1.005TrpAsn: 1.005 ± 0.217
0.377TrpPro: 0.377 ± 0.145
0.502TrpGln: 0.502 ± 0.137
0.544TrpArg: 0.544 ± 0.14
1.005TrpSer: 1.005 ± 0.227
0.628TrpThr: 0.628 ± 0.148
1.13TrpVal: 1.13 ± 0.267
0.126TrpTrp: 0.126 ± 0.111
0.544TrpTyr: 0.544 ± 0.144
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.056TyrAla: 3.056 ± 0.428
0.293TyrCys: 0.293 ± 0.11
2.135TyrAsp: 2.135 ± 0.422
2.01TyrGlu: 2.01 ± 0.362
1.968TyrPhe: 1.968 ± 0.323
2.973TyrGly: 2.973 ± 0.413
0.586TyrHis: 0.586 ± 0.165
2.261TyrIle: 2.261 ± 0.286
1.884TyrLys: 1.884 ± 0.338
2.763TyrLeu: 2.763 ± 0.331
0.754TyrMet: 0.754 ± 0.244
1.884TyrAsn: 1.884 ± 0.262
1.549TyrPro: 1.549 ± 0.245
0.963TyrGln: 0.963 ± 0.208
1.675TyrArg: 1.675 ± 0.318
1.926TyrSer: 1.926 ± 0.373
2.428TyrThr: 2.428 ± 0.462
2.01TyrVal: 2.01 ± 0.249
0.419TyrTrp: 0.419 ± 0.132
0.921TyrTyr: 0.921 ± 0.251
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 107 proteins (23886 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski