Desulfobacula toluolica (strain DSM 7467 / Tol2)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfobacterales; Desulfobacteraceae; Desulfobacula; Desulfobacula toluolica

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4189 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K0NHQ9|K0NHQ9_DESTT Putative membrane protein OS=Desulfobacula toluolica (strain DSM 7467 / Tol2) OX=651182 GN=TOL2_C03370 PE=3 SV=1
MM1 pKa = 7.68AIDD4 pKa = 4.02SNIITDD10 pKa = 4.03MGQLSALTYY19 pKa = 10.53KK20 pKa = 10.6NYY22 pKa = 10.03PSEE25 pKa = 4.33SSNSLLNKK33 pKa = 10.23GKK35 pKa = 10.1ILQGTFNIGQDD46 pKa = 3.37SFSLNNSYY54 pKa = 7.62TVKK57 pKa = 10.61DD58 pKa = 3.98YY59 pKa = 11.75ADD61 pKa = 4.12TPSDD65 pKa = 3.6MQALLLEE72 pKa = 5.1KK73 pKa = 10.38NDD75 pKa = 3.56STGNPTGEE83 pKa = 3.98YY84 pKa = 9.6IIAFRR89 pKa = 11.84GTQEE93 pKa = 3.96KK94 pKa = 10.04MDD96 pKa = 3.94IGVDD100 pKa = 4.17AIIGLANYY108 pKa = 9.42NPQFNDD114 pKa = 2.5AKK116 pKa = 11.15AFVQQMMTDD125 pKa = 3.84HH126 pKa = 6.92NISSSNLTLTGHH138 pKa = 6.01SLGAILTQSVGAVLGIKK155 pKa = 10.2GYY157 pKa = 10.95AYY159 pKa = 10.64NPYY162 pKa = 8.69GTEE165 pKa = 3.78RR166 pKa = 11.84LLTMWEE172 pKa = 4.57SYY174 pKa = 10.21TDD176 pKa = 3.69SLGEE180 pKa = 3.83ALIQVGIYY188 pKa = 9.68QVLNAFGLDD197 pKa = 3.1SSYY200 pKa = 11.92AQFAADD206 pKa = 3.84NILNVSFNDD215 pKa = 3.6CGTLNGGILSNFASEE230 pKa = 4.31LTSDD234 pKa = 3.74HH235 pKa = 6.87LGTYY239 pKa = 10.47LPVFGDD245 pKa = 3.65NEE247 pKa = 4.33GLSGHH252 pKa = 5.99SMVVLNNAISYY263 pKa = 8.1YY264 pKa = 10.73NEE266 pKa = 4.3IIAHH270 pKa = 5.59FTDD273 pKa = 3.27EE274 pKa = 4.19TDD276 pKa = 3.68YY277 pKa = 11.99DD278 pKa = 4.26DD279 pKa = 6.58LSTAYY284 pKa = 10.15ALSGEE289 pKa = 4.23NGFNRR294 pKa = 11.84LNNTFGKK301 pKa = 10.56LDD303 pKa = 3.39IAHH306 pKa = 7.19AAGNSLQFKK315 pKa = 10.13FLDD318 pKa = 3.79KK319 pKa = 11.3SSITDD324 pKa = 3.79FQSQASDD331 pKa = 3.47QAHH334 pKa = 6.61LFSLRR339 pKa = 11.84ALNPFAIIGANYY351 pKa = 10.28SIVNEE356 pKa = 4.1NGEE359 pKa = 4.42LDD361 pKa = 3.25IDD363 pKa = 4.07NYY365 pKa = 9.3SDD367 pKa = 4.47KK368 pKa = 11.15YY369 pKa = 11.0IEE371 pKa = 4.63DD372 pKa = 3.56RR373 pKa = 11.84STFLYY378 pKa = 10.82YY379 pKa = 10.57LAHH382 pKa = 7.34PDD384 pKa = 3.21EE385 pKa = 5.06KK386 pKa = 10.98LPSGEE391 pKa = 4.25DD392 pKa = 3.32TIQFTDD398 pKa = 3.06KK399 pKa = 10.8RR400 pKa = 11.84LGINTTAWKK409 pKa = 10.46SGIGVDD415 pKa = 3.99LTIRR419 pKa = 11.84DD420 pKa = 4.34YY421 pKa = 11.36FWGTEE426 pKa = 3.72VRR428 pKa = 11.84DD429 pKa = 3.76EE430 pKa = 4.52FGSNGNTGDD439 pKa = 3.5DD440 pKa = 3.72HH441 pKa = 8.14LYY443 pKa = 11.39GMGGNDD449 pKa = 3.32TLKK452 pKa = 10.93GYY454 pKa = 10.57GGEE457 pKa = 4.34DD458 pKa = 3.81YY459 pKa = 11.06IEE461 pKa = 4.67GGEE464 pKa = 4.55GQDD467 pKa = 3.14TMYY470 pKa = 11.25GGGDD474 pKa = 3.25KK475 pKa = 10.38DD476 pKa = 3.35TFYY479 pKa = 10.84IQGEE483 pKa = 4.09DD484 pKa = 3.44DD485 pKa = 5.58DD486 pKa = 5.86YY487 pKa = 11.75DD488 pKa = 3.6IFNGGDD494 pKa = 3.48HH495 pKa = 6.96NEE497 pKa = 3.92DD498 pKa = 3.91TILGSEE504 pKa = 4.35GNDD507 pKa = 3.58TIRR510 pKa = 11.84VHH512 pKa = 7.1DD513 pKa = 4.22FSGEE517 pKa = 4.0NTVEE521 pKa = 4.31IIDD524 pKa = 3.74GRR526 pKa = 11.84GGEE529 pKa = 4.08NVIAGTGMVDD539 pKa = 3.9TIDD542 pKa = 3.44MSGTALIDD550 pKa = 3.08IDD552 pKa = 4.84RR553 pKa = 11.84IEE555 pKa = 5.03GGDD558 pKa = 3.62GADD561 pKa = 3.73TIKK564 pKa = 11.13GCLADD569 pKa = 3.61DD570 pKa = 4.87TIYY573 pKa = 11.07GGSKK577 pKa = 10.42DD578 pKa = 3.86QIEE581 pKa = 4.78DD582 pKa = 3.49NAVDD586 pKa = 3.79QLEE589 pKa = 4.58GGAGNDD595 pKa = 2.77IYY597 pKa = 11.51YY598 pKa = 10.54AGTGDD603 pKa = 5.01VINDD607 pKa = 3.45LDD609 pKa = 3.94GRR611 pKa = 11.84GTVWFEE617 pKa = 3.44GRR619 pKa = 11.84EE620 pKa = 4.04LSGLTWTGLSPDD632 pKa = 3.54SNIYY636 pKa = 10.29SDD638 pKa = 4.19SDD640 pKa = 3.35EE641 pKa = 4.53NYY643 pKa = 10.43YY644 pKa = 11.44ALFDD648 pKa = 3.79NTSNTLIVSHH658 pKa = 6.96SSTNHH663 pKa = 5.52YY664 pKa = 10.44IKK666 pKa = 10.32IEE668 pKa = 4.01NFSDD672 pKa = 3.5GTLGLTLEE680 pKa = 5.44DD681 pKa = 3.81YY682 pKa = 8.91TPPSDD687 pKa = 4.25YY688 pKa = 10.82DD689 pKa = 3.46YY690 pKa = 11.1TLIGSADD697 pKa = 3.92DD698 pKa = 5.26DD699 pKa = 4.31EE700 pKa = 7.07SDD702 pKa = 3.54YY703 pKa = 11.65HH704 pKa = 7.14PDD706 pKa = 3.51YY707 pKa = 11.72DD708 pKa = 4.92LGTYY712 pKa = 10.12SYY714 pKa = 11.59GFGDD718 pKa = 3.91NADD721 pKa = 3.27ITADD725 pKa = 3.34MSTSISLEE733 pKa = 3.85IYY735 pKa = 10.18GGAGSDD741 pKa = 4.17YY742 pKa = 10.96ILGLPWDD749 pKa = 4.93DD750 pKa = 3.78YY751 pKa = 11.95LNGGGGDD758 pKa = 3.63DD759 pKa = 4.88HH760 pKa = 7.98IVSNSNSTMAPDD772 pKa = 3.3IVGDD776 pKa = 3.9VMDD779 pKa = 5.36GGDD782 pKa = 4.35GNDD785 pKa = 4.9LIQDD789 pKa = 4.02TGNVGSVMLGGAGFDD804 pKa = 3.15ILNGYY809 pKa = 8.96RR810 pKa = 11.84GNDD813 pKa = 3.32TMSGGSQTDD822 pKa = 3.49VLTGHH827 pKa = 7.26AGDD830 pKa = 5.47DD831 pKa = 3.89YY832 pKa = 11.78LSGGDD837 pKa = 4.01GNDD840 pKa = 3.22VLLGDD845 pKa = 5.34NDD847 pKa = 4.67LFWTNSIMGMLGPNMVDD864 pKa = 3.72FTFDD868 pKa = 3.07QAGWITDD875 pKa = 3.51VNFNGAMSVKK885 pKa = 10.5DD886 pKa = 3.47GDD888 pKa = 4.44TITVTGIGGGDD899 pKa = 3.17IYY901 pKa = 11.32FDD903 pKa = 3.48IPAGNDD909 pKa = 3.28FLDD912 pKa = 4.14GGNGNDD918 pKa = 4.02HH919 pKa = 7.1LYY921 pKa = 10.94DD922 pKa = 3.4

Molecular weight: 99.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K0NQ50|K0NQ50_DESTT BamI2: uncharacterized anaerobic dehydrogenase 2Fe-2S and 4Fe-4S ferredoxin iron-sulfur protein OS=Desulfobacula toluolica (strain DSM 7467 / Tol2) OX=651182 GN=bamI2 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNRR10 pKa = 11.84KK11 pKa = 7.64RR12 pKa = 11.84TRR14 pKa = 11.84KK15 pKa = 9.43HH16 pKa = 4.96GFRR19 pKa = 11.84KK20 pKa = 10.03RR21 pKa = 11.84MSTPSGRR28 pKa = 11.84RR29 pKa = 11.84VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.86GRR39 pKa = 11.84KK40 pKa = 8.94KK41 pKa = 10.21LTVV44 pKa = 3.1

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4189

0

4189

1459019

32

3944

348.3

39.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.927 ± 0.04

1.381 ± 0.017

5.765 ± 0.031

6.2 ± 0.036

4.827 ± 0.028

6.696 ± 0.058

2.046 ± 0.015

8.156 ± 0.036

7.804 ± 0.043

9.475 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.756 ± 0.016

4.645 ± 0.026

3.849 ± 0.023

3.489 ± 0.02

4.265 ± 0.025

6.227 ± 0.028

5.249 ± 0.025

6.165 ± 0.026

0.96 ± 0.014

3.118 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski