Salipiger mucosus DSM 16094
Average proteome isoelectric point is 6.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5583 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S9R544|S9R544_9RHOB Peptide deformylase OS=Salipiger mucosus DSM 16094 OX=1123237 GN=def PE=3 SV=1
MM1 pKa = 7.86 PEE3 pKa = 3.5 AFLRR7 pKa = 11.84 GVANLVRR14 pKa = 11.84 GFDD17 pKa = 3.63 RR18 pKa = 11.84 LVSVAMSTHH27 pKa = 6.89 PNGQANTQKK36 pKa = 10.75 GEE38 pKa = 4.3 SYY40 pKa = 9.37 MATYY44 pKa = 10.22 YY45 pKa = 11.17 VSTTGSNANDD55 pKa = 3.87 GSEE58 pKa = 4.08 SSPFATINHH67 pKa = 6.26 AVSSGLQPGDD77 pKa = 3.37 TVLVKK82 pKa = 10.39 PGTYY86 pKa = 9.3 RR87 pKa = 11.84 EE88 pKa = 4.09 SVYY91 pKa = 11.35 VNIDD95 pKa = 2.96 GSAAGDD101 pKa = 3.15 ITIRR105 pKa = 11.84 SEE107 pKa = 4.29 TPGAASIQPGSSGNGFTIEE126 pKa = 4.06 GSYY129 pKa = 8.82 ITIDD133 pKa = 3.23 GFEE136 pKa = 4.21 ITGSAYY142 pKa = 10.35 HH143 pKa = 6.99 GIEE146 pKa = 4.23 AQNSHH151 pKa = 7.18 HH152 pKa = 6.98 ISMLNNISHH161 pKa = 7.62 DD162 pKa = 3.79 NGASGLSAIWGEE174 pKa = 4.18 FYY176 pKa = 10.78 LIEE179 pKa = 4.98 GNTTYY184 pKa = 11.45 GNAASDD190 pKa = 3.8 WFSGISVYY198 pKa = 8.77 EE199 pKa = 3.87 NRR201 pKa = 11.84 NITGDD206 pKa = 3.72 TEE208 pKa = 4.33 TTGYY212 pKa = 8.48 RR213 pKa = 11.84 TIIRR217 pKa = 11.84 NNVSYY222 pKa = 11.51 DD223 pKa = 3.2 NVTEE227 pKa = 4.35 NGQHH231 pKa = 5.92 TDD233 pKa = 3.01 GNGIIIDD240 pKa = 4.52 DD241 pKa = 4.14 FQSTQNSAFPSYY253 pKa = 10.25 TYY255 pKa = 7.92 PTLVEE260 pKa = 4.07 NNLVYY265 pKa = 10.91 EE266 pKa = 4.3 NGGKK270 pKa = 10.14 GIQVIWSDD278 pKa = 3.24 NVTVRR283 pKa = 11.84 GNTAYY288 pKa = 10.66 HH289 pKa = 6.38 NNQDD293 pKa = 3.83 LQNTGTWRR301 pKa = 11.84 GEE303 pKa = 3.68 ISNSQSSNNTFVNNIAVVDD322 pKa = 4.15 PSVHH326 pKa = 6.62 PEE328 pKa = 3.36 NTAIDD333 pKa = 3.72 NNSYY337 pKa = 11.07 GGYY340 pKa = 9.05 TNDD343 pKa = 3.05 NVVWANNLTYY353 pKa = 10.45 TGTAGEE359 pKa = 4.59 ASVKK363 pKa = 9.89 TDD365 pKa = 3.61 GGNAMPSAADD375 pKa = 3.72 GNLLGVDD382 pKa = 4.38 PGFIDD387 pKa = 3.7 PGNDD391 pKa = 2.82 FRR393 pKa = 11.84 LSSGSVAIDD402 pKa = 3.93 AGTDD406 pKa = 3.48 DD407 pKa = 4.45 YY408 pKa = 12.1 GLASVDD414 pKa = 3.68 LEE416 pKa = 4.04 GTARR420 pKa = 11.84 VEE422 pKa = 4.17 GVVDD426 pKa = 3.6 IGAYY430 pKa = 9.79 EE431 pKa = 4.33 RR432 pKa = 11.84 DD433 pKa = 3.6 SSGTPTPPANTAPEE447 pKa = 4.12 ATNDD451 pKa = 3.51 SGFSTTEE458 pKa = 3.55 DD459 pKa = 3.19 TAATIATADD468 pKa = 3.72 LRR470 pKa = 11.84 ANDD473 pKa = 3.86 SDD475 pKa = 4.51 ADD477 pKa = 3.86 GDD479 pKa = 4.27 ALTVTSVSGAVNGTVSLASASSVVFTPDD507 pKa = 2.62 AGYY510 pKa = 10.99 SGMASFDD517 pKa = 3.64 YY518 pKa = 10.32 TVSDD522 pKa = 4.06 GAGGTDD528 pKa = 3.15 TATVSIEE535 pKa = 3.84 VTAASAPPSNTAPVATDD552 pKa = 3.32 DD553 pKa = 4.26 SGFATSEE560 pKa = 3.92 DD561 pKa = 3.73 TALSLKK567 pKa = 10.6 PSMLKK572 pKa = 10.98 DD573 pKa = 3.27 NDD575 pKa = 3.28 TDD577 pKa = 4.17 ADD579 pKa = 4.21 GDD581 pKa = 4.24 SLTITNVSGAVNGTVSLAADD601 pKa = 3.78 EE602 pKa = 4.77 TVVFTPDD609 pKa = 2.81 AGFTGMARR617 pKa = 11.84 FDD619 pKa = 3.66 YY620 pKa = 10.14 TISDD624 pKa = 4.01 GAGGTDD630 pKa = 3.2 TAQAEE635 pKa = 4.63 IQVSTAEE642 pKa = 4.22 VTPPAPPEE650 pKa = 4.3 DD651 pKa = 4.31 DD652 pKa = 4.14 EE653 pKa = 4.61 EE654 pKa = 6.07 AGSFSIWDD662 pKa = 3.51 SSATPDD668 pKa = 4.27 IMADD672 pKa = 3.19 SDD674 pKa = 3.99 TAAVTLGLRR683 pKa = 11.84 FTADD687 pKa = 2.29 VDD689 pKa = 3.88 AALEE693 pKa = 3.88 ALRR696 pKa = 11.84 LYY698 pKa = 10.8 VSEE701 pKa = 5.28 ANTGTQSINLWSDD714 pKa = 2.48 SGTRR718 pKa = 11.84 LGQATAEE725 pKa = 4.27 VTEE728 pKa = 4.9 SGWSTVSFDD737 pKa = 5.48 KK738 pKa = 10.8 PLQLEE743 pKa = 4.01 AGEE746 pKa = 5.17 DD747 pKa = 4.24 YY748 pKa = 10.57 IASYY752 pKa = 9.49 YY753 pKa = 10.15 APNGGYY759 pKa = 9.52 VVSEE763 pKa = 5.08 DD764 pKa = 4.11 YY765 pKa = 10.82 FDD767 pKa = 4.0 SASDD771 pKa = 3.44 EE772 pKa = 4.48 GPISLEE778 pKa = 4.16 ANSGVYY784 pKa = 10.1 SYY786 pKa = 11.82 GSGGSSFPDD795 pKa = 2.97 QSYY798 pKa = 10.8 AFSNYY803 pKa = 7.35 WVDD806 pKa = 4.23 VVLTPDD812 pKa = 3.19 TATDD816 pKa = 3.54 VAGDD820 pKa = 3.78 NVIGEE825 pKa = 4.54 TGTVVAGQAGAGDD838 pKa = 3.92 WTSVTFDD845 pKa = 4.1 TPLEE849 pKa = 4.5 DD850 pKa = 3.8 PSVVMGGLTGNGANPYY866 pKa = 6.71 TVRR869 pKa = 11.84 VRR871 pKa = 11.84 NVTDD875 pKa = 3.03 TGFEE879 pKa = 4.08 YY880 pKa = 10.72 QIDD883 pKa = 4.15 EE884 pKa = 4.6 YY885 pKa = 11.24 EE886 pKa = 4.54 YY887 pKa = 11.23 LDD889 pKa = 3.95 DD890 pKa = 3.31 WHH892 pKa = 6.0 TTEE895 pKa = 4.8 EE896 pKa = 4.29 ISWLAVEE903 pKa = 5.02 KK904 pKa = 9.54 GTHH907 pKa = 4.97 VLSDD911 pKa = 3.33 GRR913 pKa = 11.84 TISAGDD919 pKa = 3.6 ASVGGSFAGISFDD932 pKa = 5.67 DD933 pKa = 4.5 GAFDD937 pKa = 3.62 SAPVVLGQAVGEE949 pKa = 4.16 ANAMAVTDD957 pKa = 3.98 RR958 pKa = 11.84 LRR960 pKa = 11.84 SVSEE964 pKa = 3.9 TGFEE968 pKa = 3.84 ARR970 pKa = 11.84 LFQQEE975 pKa = 4.09 AATGSIEE982 pKa = 4.98 AEE984 pKa = 4.07 GFDD987 pKa = 4.59 WIAVGQGGSATDD999 pKa = 3.6 GALAGTSGNMVDD1011 pKa = 4.56 HH1012 pKa = 7.22 RR1013 pKa = 11.84 PTTVDD1018 pKa = 3.63 FDD1020 pKa = 4.46 GSFSGEE1026 pKa = 4.21 DD1027 pKa = 3.41 FVMVTDD1033 pKa = 3.96 MQTTNGWDD1041 pKa = 3.24 VATLQTASLDD1051 pKa = 3.86 DD1052 pKa = 3.91 EE1053 pKa = 5.58 SMVLRR1058 pKa = 11.84 VLEE1061 pKa = 4.09 EE1062 pKa = 3.7 TSRR1065 pKa = 11.84 DD1066 pKa = 3.39 GEE1068 pKa = 4.3 VRR1070 pKa = 11.84 HH1071 pKa = 5.91 VDD1073 pKa = 3.06 EE1074 pKa = 5.41 DD1075 pKa = 3.69 AGFVGFEE1082 pKa = 3.93 TGLIEE1087 pKa = 4.17 GTPFEE1092 pKa = 4.89 SDD1094 pKa = 3.03 TVLL1097 pKa = 4.05
Molecular weight: 114.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.643
IPC_protein 3.681
Toseland 3.452
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.605
Rodwell 3.503
Grimsley 3.363
Solomon 3.668
Lehninger 3.63
Nozaki 3.783
DTASelect 4.037
Thurlkill 3.503
EMBOSS 3.617
Sillero 3.808
Patrickios 1.558
IPC_peptide 3.668
IPC2_peptide 3.783
IPC2.peptide.svr19 3.729
Protein with the highest isoelectric point:
>tr|S9Q315|S9Q315_9RHOB L-fuconolactone hydrolase OS=Salipiger mucosus DSM 16094 OX=1123237 GN=Salmuc_01176 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.44 AGRR28 pKa = 11.84 KK29 pKa = 8.46 VLNARR34 pKa = 11.84 RR35 pKa = 11.84 ARR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 8.79 NLSAA44 pKa = 4.73
Molecular weight: 5.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.523
IPC2_protein 11.242
IPC_protein 12.837
Toseland 13.013
ProMoST 13.495
Dawson 13.013
Bjellqvist 12.998
Wikipedia 13.481
Rodwell 12.676
Grimsley 13.042
Solomon 13.495
Lehninger 13.408
Nozaki 13.013
DTASelect 12.998
Thurlkill 13.013
EMBOSS 13.51
Sillero 13.013
Patrickios 12.398
IPC_peptide 13.51
IPC2_peptide 12.486
IPC2.peptide.svr19 9.177
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5583
0
5583
1630836
37
2149
292.1
31.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.472 ± 0.049
0.893 ± 0.011
6.174 ± 0.033
6.521 ± 0.029
3.564 ± 0.022
8.975 ± 0.038
2.091 ± 0.017
4.642 ± 0.028
2.614 ± 0.029
9.909 ± 0.037
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.741 ± 0.018
2.294 ± 0.02
5.295 ± 0.025
3.003 ± 0.02
7.493 ± 0.041
5.08 ± 0.026
5.399 ± 0.024
7.295 ± 0.029
1.402 ± 0.015
2.143 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here