Blackberry vein banding-associated virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Martellivirales; Closteroviridae; Ampelovirus

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|S5U8X6|S5U8X6_9CLOS RNA-dependent RNA polymerase 1b OS=Blackberry vein banding-associated virus OX=1381464 PE=4 SV=1
MM1 pKa = 7.44EE2 pKa = 5.87FKK4 pKa = 10.54PVTIKK9 pKa = 10.53VSKK12 pKa = 9.89VGSGEE17 pKa = 3.73IVPYY21 pKa = 10.1NVEE24 pKa = 4.35SIDD27 pKa = 3.67MVSYY31 pKa = 11.55ALFDD35 pKa = 3.54NVKK38 pKa = 10.36DD39 pKa = 3.86FRR41 pKa = 11.84VVSKK45 pKa = 10.41EE46 pKa = 3.8DD47 pKa = 3.36SSTLSEE53 pKa = 4.67GYY55 pKa = 8.24STNSAFILNPYY66 pKa = 9.36KK67 pKa = 10.64IFGAVFDD74 pKa = 4.72KK75 pKa = 10.88SGKK78 pKa = 10.37AIATDD83 pKa = 3.43VASVFRR89 pKa = 11.84VYY91 pKa = 10.9DD92 pKa = 3.02LHH94 pKa = 8.09NYY96 pKa = 8.35VVEE99 pKa = 4.6KK100 pKa = 10.56IGDD103 pKa = 3.23IALYY107 pKa = 10.53KK108 pKa = 10.2RR109 pKa = 11.84VTYY112 pKa = 10.47LCEE115 pKa = 3.9YY116 pKa = 10.17DD117 pKa = 3.75SLGGSVEE124 pKa = 4.4TITVKK129 pKa = 10.27VVKK132 pKa = 10.33DD133 pKa = 3.67DD134 pKa = 3.68GTIVDD139 pKa = 3.81VVIDD143 pKa = 4.1NNHH146 pKa = 6.01KK147 pKa = 10.31CWAGLKK153 pKa = 9.84KK154 pKa = 10.73DD155 pKa = 4.49LVWEE159 pKa = 4.12QNQARR164 pKa = 11.84TLKK167 pKa = 10.94GKK169 pKa = 10.12DD170 pKa = 3.17VLDD173 pKa = 3.82AVLLLEE179 pKa = 4.79NYY181 pKa = 9.93PGFKK185 pKa = 10.39

Molecular weight: 20.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|S5TIV9|S5TIV9_9CLOS Minor coat protein OS=Blackberry vein banding-associated virus OX=1381464 PE=4 SV=1
MM1 pKa = 6.58VRR3 pKa = 11.84KK4 pKa = 9.93VFLPCNVEE12 pKa = 3.73GWRR15 pKa = 11.84LRR17 pKa = 11.84RR18 pKa = 11.84WLYY21 pKa = 8.73ISVYY25 pKa = 9.73IVSTACTASLVLLGCFPPRR44 pKa = 11.84SFYY47 pKa = 10.26LTWW50 pKa = 4.82

Molecular weight: 5.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

5363

44

2384

487.5

54.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.936 ± 0.388

1.995 ± 0.285

5.445 ± 0.377

5.687 ± 0.247

4.139 ± 0.327

6.769 ± 0.452

1.287 ± 0.288

4.233 ± 0.278

6.004 ± 0.258

9.64 ± 0.732

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.2 ± 0.306

3.729 ± 0.438

3.841 ± 0.455

2.014 ± 0.207

6.47 ± 0.443

8.298 ± 0.309

6.172 ± 0.33

10.535 ± 0.437

0.746 ± 0.1

3.86 ± 0.188
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski