Wuhan insect virus 21

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KGP6|A0A1L3KGP6_9VIRU RNA-directed RNA polymerase OS=Wuhan insect virus 21 OX=1923725 PE=4 SV=1
MM1 pKa = 7.72EE2 pKa = 5.59GPHH5 pKa = 6.29EE6 pKa = 4.46SSPRR10 pKa = 11.84HH11 pKa = 5.91CDD13 pKa = 3.39APTCEE18 pKa = 4.18QPLSEE23 pKa = 4.81GLLSPIRR30 pKa = 11.84ASVQQSYY37 pKa = 9.26GQSHH41 pKa = 6.5SDD43 pKa = 3.18YY44 pKa = 10.88SLRR47 pKa = 11.84HH48 pKa = 4.91MRR50 pKa = 11.84NHH52 pKa = 7.22GSRR55 pKa = 11.84PCQRR59 pKa = 11.84PTVRR63 pKa = 11.84FHH65 pKa = 6.36QEE67 pKa = 3.0SGRR70 pKa = 11.84RR71 pKa = 11.84PDD73 pKa = 3.41QDD75 pKa = 2.79AHH77 pKa = 7.96SPICRR82 pKa = 11.84GVPEE86 pKa = 4.78GAHH89 pKa = 6.39KK90 pKa = 10.63ADD92 pKa = 4.97SRR94 pKa = 11.84GCCHH98 pKa = 6.86GRR100 pKa = 11.84GCTGKK105 pKa = 10.58RR106 pKa = 11.84STCRR110 pKa = 11.84EE111 pKa = 3.58NHH113 pKa = 5.43VSIGHH118 pKa = 6.08HH119 pKa = 6.11PVLLHH124 pKa = 6.46HH125 pKa = 6.9PGVLDD130 pKa = 3.13MGIRR134 pKa = 11.84LLCCSTDD141 pKa = 3.44NAATATQARR150 pKa = 11.84GPPFDD155 pKa = 4.2SGCLLRR161 pKa = 11.84DD162 pKa = 3.37IRR164 pKa = 11.84DD165 pKa = 3.83DD166 pKa = 4.15GQGHH170 pKa = 6.17RR171 pKa = 11.84PHH173 pKa = 6.54RR174 pKa = 11.84TCRR177 pKa = 11.84MSTCAVLALIALTLLTISRR196 pKa = 11.84PTNCRR201 pKa = 11.84EE202 pKa = 3.51ISGATVIPGDD212 pKa = 3.43GKK214 pKa = 8.88TYY216 pKa = 10.74CSTASSGFTLRR227 pKa = 11.84TATLVSRR234 pKa = 11.84ITGKK238 pKa = 8.34HH239 pKa = 3.97TSAVLVPRR247 pKa = 11.84GLVTNDD253 pKa = 2.96KK254 pKa = 11.05SYY256 pKa = 11.25LSNDD260 pKa = 3.11RR261 pKa = 11.84FEE263 pKa = 5.13VWDD266 pKa = 3.97FDD268 pKa = 3.83KK269 pKa = 11.4QLVNTGTFISWPFEE283 pKa = 3.91HH284 pKa = 7.43PPRR287 pKa = 11.84DD288 pKa = 3.89CLTLLEE294 pKa = 4.73TNTLAHH300 pKa = 6.61SDD302 pKa = 4.03CPLSRR307 pKa = 11.84SCSNVAYY314 pKa = 10.03HH315 pKa = 6.33QLDD318 pKa = 3.82VKK320 pKa = 10.58WYY322 pKa = 8.39GKK324 pKa = 10.58EE325 pKa = 3.92SGLCTGTLLPHH336 pKa = 4.63VTVFNGTATNRR347 pKa = 11.84HH348 pKa = 5.2GTLSTDD354 pKa = 2.89WGPLYY359 pKa = 10.99SFVDD363 pKa = 3.39AGSWTDD369 pKa = 3.25VYY371 pKa = 11.62NVLPSLRR378 pKa = 11.84LLRR381 pKa = 11.84LPGRR385 pKa = 11.84DD386 pKa = 2.65IMFSYY391 pKa = 10.94DD392 pKa = 2.91ATLNLVALKK401 pKa = 10.23SDD403 pKa = 3.52PYY405 pKa = 11.13KK406 pKa = 10.58PVTVPVIIQPYY417 pKa = 7.41YY418 pKa = 11.18AEE420 pKa = 4.59GKK422 pKa = 9.45GQEE425 pKa = 3.75FCMWVNPPEE434 pKa = 4.24RR435 pKa = 11.84SRR437 pKa = 11.84ALVIYY442 pKa = 9.62DD443 pKa = 3.96GPTLTEE449 pKa = 4.27WPPFTPVLAVHH460 pKa = 7.17DD461 pKa = 5.05LDD463 pKa = 4.57TYY465 pKa = 10.94QPPICLDD472 pKa = 3.46YY473 pKa = 10.93QPRR476 pKa = 11.84PDD478 pKa = 3.61MPLNGHH484 pKa = 6.21RR485 pKa = 11.84RR486 pKa = 11.84VTMGDD491 pKa = 3.06GDD493 pKa = 3.9VGRR496 pKa = 11.84VVQDD500 pKa = 3.78TPALACAPFKK510 pKa = 10.77HH511 pKa = 6.3ISSYY515 pKa = 10.9SVPGSIWLKK524 pKa = 10.78SMLAFVIEE532 pKa = 4.52SIEE535 pKa = 4.35TVVAAAFRR543 pKa = 11.84ATITVTHH550 pKa = 6.76DD551 pKa = 4.7LIAEE555 pKa = 4.1FNDD558 pKa = 2.84RR559 pKa = 11.84FMLFEE564 pKa = 4.61ISLLAGYY571 pKa = 8.33VMWHH575 pKa = 6.04TDD577 pKa = 2.82SMIRR581 pKa = 11.84TAVVCGMYY589 pKa = 10.01TLLFGVQRR597 pKa = 11.84NPAQSPP603 pKa = 3.5

Molecular weight: 66.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KGV1|A0A1L3KGV1_9VIRU Chropara_Vmeth domain-containing protein OS=Wuhan insect virus 21 OX=1923725 PE=4 SV=1
MM1 pKa = 7.89PRR3 pKa = 11.84RR4 pKa = 11.84QAVTSRR10 pKa = 11.84PWRR13 pKa = 11.84GLTRR17 pKa = 11.84AAPVIATRR25 pKa = 11.84RR26 pKa = 11.84RR27 pKa = 11.84VSSRR31 pKa = 11.84SAKK34 pKa = 10.56DD35 pKa = 2.95FLARR39 pKa = 11.84LGQAFNRR46 pKa = 11.84VTANPIAIIAFAICAIMAVDD66 pKa = 3.69HH67 pKa = 6.98AKK69 pKa = 10.88DD70 pKa = 3.73PLSDD74 pKa = 4.11SIKK77 pKa = 10.42KK78 pKa = 9.97VADD81 pKa = 3.79ALIKK85 pKa = 10.22MPTLQFVGEE94 pKa = 4.09YY95 pKa = 9.86LKK97 pKa = 10.9EE98 pKa = 4.36HH99 pKa = 6.93IKK101 pKa = 8.68QTVGAVAMGAAVLVSARR118 pKa = 11.84PAEE121 pKa = 4.11KK122 pKa = 8.21TTYY125 pKa = 11.13LLGTTLFSYY134 pKa = 10.3IIPEE138 pKa = 3.97YY139 pKa = 10.65SIWAYY144 pKa = 10.41GCYY147 pKa = 9.72AAALTMLLQLRR158 pKa = 11.84RR159 pKa = 11.84LEE161 pKa = 4.2DD162 pKa = 3.3RR163 pKa = 11.84LLIAGACFVIYY174 pKa = 11.07VMTVKK179 pKa = 10.12DD180 pKa = 3.66TDD182 pKa = 3.08HH183 pKa = 6.64TARR186 pKa = 11.84VGG188 pKa = 3.27

Molecular weight: 20.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

2354

188

919

588.5

65.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.539 ± 0.961

2.421 ± 0.439

5.862 ± 0.403

4.46 ± 0.505

3.314 ± 0.369

6.415 ± 0.816

3.866 ± 0.587

5.055 ± 0.402

3.441 ± 0.439

7.986 ± 0.985

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.997 ± 0.429

2.974 ± 0.278

6.457 ± 0.394

3.144 ± 0.099

8.539 ± 0.499

7.264 ± 0.363

7.392 ± 0.347

6.542 ± 0.322

1.359 ± 0.143

2.974 ± 0.088
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski