Trinickia soli
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5348 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2N7VPZ8|A0A2N7VPZ8_9BURK Gamma carbonic anhydrase family protein OS=Trinickia soli OX=380675 GN=C0Z19_21635 PE=4 SV=1
MM1 pKa = 6.97 NTYY4 pKa = 9.6 CACPYY9 pKa = 10.75 NLDD12 pKa = 3.48 AVGFCFGTFDD22 pKa = 5.01 EE23 pKa = 4.53 YY24 pKa = 11.22 LQRR27 pKa = 11.84 AAACADD33 pKa = 3.3 SFGNVVEE40 pKa = 4.17 EE41 pKa = 5.08 FEE43 pKa = 4.21 IQFIDD48 pKa = 4.24 GDD50 pKa = 3.92 AADD53 pKa = 4.42 AQLFAAFGVDD63 pKa = 4.01 QAGLSAWFDD72 pKa = 3.4 RR73 pKa = 11.84 VEE75 pKa = 4.13 DD76 pKa = 3.75 LRR78 pKa = 11.84 SEE80 pKa = 4.26 VKK82 pKa = 10.43 AAAFYY87 pKa = 10.43 LASRR91 pKa = 11.84 CGQTVGDD98 pKa = 5.0 AIDD101 pKa = 3.61 NAEE104 pKa = 4.15 DD105 pKa = 3.44 VIIRR109 pKa = 11.84 KK110 pKa = 9.94 GEE112 pKa = 3.91 LADD115 pKa = 3.82 AAAEE119 pKa = 4.07 QFDD122 pKa = 5.0 DD123 pKa = 5.23 FYY125 pKa = 11.68 SDD127 pKa = 3.7 LPRR130 pKa = 11.84 GVRR133 pKa = 11.84 MYY135 pKa = 11.56 VDD137 pKa = 3.51 IEE139 pKa = 4.18 AYY141 pKa = 10.33 ARR143 pKa = 11.84 DD144 pKa = 3.86 CRR146 pKa = 11.84 LSGDD150 pKa = 3.42 LDD152 pKa = 3.55 EE153 pKa = 6.62 FEE155 pKa = 5.57 FAGQAYY161 pKa = 7.32 TVLNANEE168 pKa = 4.25 CC169 pKa = 3.77
Molecular weight: 18.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.745
IPC2_protein 3.757
IPC_protein 3.757
Toseland 3.541
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.694
Rodwell 3.592
Grimsley 3.452
Solomon 3.745
Lehninger 3.706
Nozaki 3.872
DTASelect 4.101
Thurlkill 3.592
EMBOSS 3.706
Sillero 3.884
Patrickios 0.604
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.798
Protein with the highest isoelectric point:
>tr|A0A2N7W774|A0A2N7W774_9BURK Uncharacterized protein OS=Trinickia soli OX=380675 GN=C0Z19_09760 PE=4 SV=1
MM1 pKa = 7.64 HH2 pKa = 7.19 KK3 pKa = 10.33 RR4 pKa = 11.84 LASRR8 pKa = 11.84 HH9 pKa = 4.63 NRR11 pKa = 11.84 MTLSPARR18 pKa = 11.84 PVRR21 pKa = 11.84 RR22 pKa = 11.84 ASMRR26 pKa = 11.84 ALRR29 pKa = 11.84 HH30 pKa = 4.64 HH31 pKa = 7.33 AARR34 pKa = 11.84 TRR36 pKa = 11.84 ALDD39 pKa = 3.53 TRR41 pKa = 11.84 RR42 pKa = 11.84 SATNGSHH49 pKa = 6.85 DD50 pKa = 3.67 RR51 pKa = 11.84 SGGLLRR57 pKa = 11.84 AWLRR61 pKa = 11.84 AVLSALRR68 pKa = 11.84 TGTRR72 pKa = 11.84 ARR74 pKa = 11.84 RR75 pKa = 11.84 VQLRR79 pKa = 11.84 RR80 pKa = 11.84 LLRR83 pKa = 11.84 RR84 pKa = 11.84 ATIRR88 pKa = 11.84 RR89 pKa = 11.84 STLARR94 pKa = 11.84 AKK96 pKa = 10.4 RR97 pKa = 11.84 SAQANGNRR105 pKa = 11.84 LPRR108 pKa = 11.84 RR109 pKa = 11.84 LSASTGWFTFGSRR122 pKa = 3.53
Molecular weight: 13.94 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.516
IPC2_protein 11.199
IPC_protein 12.808
Toseland 12.969
ProMoST 13.466
Dawson 12.969
Bjellqvist 12.969
Wikipedia 13.451
Rodwell 12.501
Grimsley 13.013
Solomon 13.466
Lehninger 13.364
Nozaki 12.969
DTASelect 12.969
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.208
IPC_peptide 13.481
IPC2_peptide 12.457
IPC2.peptide.svr19 9.2
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5348
0
5348
1751137
21
3222
327.4
35.52
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.109 ± 0.051
0.963 ± 0.01
5.433 ± 0.024
5.234 ± 0.033
3.617 ± 0.02
8.136 ± 0.037
2.342 ± 0.017
4.588 ± 0.02
2.958 ± 0.026
10.096 ± 0.042
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.299 ± 0.014
2.654 ± 0.024
5.124 ± 0.029
3.605 ± 0.022
7.264 ± 0.042
5.712 ± 0.026
5.422 ± 0.034
7.663 ± 0.028
1.352 ± 0.013
2.431 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here