Mosquito VEM virus SDRBAJ
Average proteome isoelectric point is 7.52
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|F6KIE6|F6KIE6_9VIRU Uncharacterized protein OS=Mosquito VEM virus SDRBAJ OX=1034806 PE=4 SV=1
MM1 pKa = 8.2 DD2 pKa = 3.71 MRR4 pKa = 11.84 DD5 pKa = 3.61 NPRR8 pKa = 11.84 SLSMSSTDD16 pKa = 3.38 GSPEE20 pKa = 3.35 ISSRR24 pKa = 11.84 DD25 pKa = 3.59 SQIATPSLWRR35 pKa = 11.84 PKK37 pKa = 10.59 AEE39 pKa = 4.51 PFNSWRR45 pKa = 11.84 RR46 pKa = 11.84 EE47 pKa = 4.07 SLSPPTARR55 pKa = 11.84 RR56 pKa = 11.84 SPGINQDD63 pKa = 2.72 WAQWDD68 pKa = 3.94 EE69 pKa = 4.3 DD70 pKa = 4.61 FKK72 pKa = 11.45 HH73 pKa = 6.19 LWGTYY78 pKa = 9.8 SMSGPQSAMKK88 pKa = 10.37 QPIANNWMKK97 pKa = 10.87 SDD99 pKa = 3.78 PEE101 pKa = 4.33 VIHH104 pKa = 6.48 FLEE107 pKa = 4.7 EE108 pKa = 3.68 QWEE111 pKa = 3.97 QDD113 pKa = 3.22 PFEE116 pKa = 4.64 VKK118 pKa = 10.36 SVTWW122 pKa = 3.44
Molecular weight: 14.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.031
IPC2_protein 4.991
IPC_protein 4.851
Toseland 4.724
ProMoST 4.965
Dawson 4.813
Bjellqvist 4.952
Wikipedia 4.685
Rodwell 4.711
Grimsley 4.647
Solomon 4.813
Lehninger 4.762
Nozaki 4.927
DTASelect 5.092
Thurlkill 4.736
EMBOSS 4.711
Sillero 4.991
Patrickios 4.533
IPC_peptide 4.813
IPC2_peptide 4.978
IPC2.peptide.svr19 5.005
Protein with the highest isoelectric point:
>tr|F6KIE6|F6KIE6_9VIRU Uncharacterized protein OS=Mosquito VEM virus SDRBAJ OX=1034806 PE=4 SV=1
MM1 pKa = 7.66 APGKK5 pKa = 10.29 KK6 pKa = 9.45 SLHH9 pKa = 5.89 SSEE12 pKa = 4.83 SNTIRR17 pKa = 11.84 EE18 pKa = 4.47 HH19 pKa = 5.39 YY20 pKa = 7.53 QTLVVFGAGGTSLKK34 pKa = 10.8 LIGEE38 pKa = 4.82 IFRR41 pKa = 11.84 SLYY44 pKa = 9.62 SQIPIWLRR52 pKa = 11.84 GIIIGKK58 pKa = 8.64 YY59 pKa = 8.56 PISIFLFRR67 pKa = 11.84 SRR69 pKa = 11.84 FRR71 pKa = 11.84 HH72 pKa = 4.32 NMIWEE77 pKa = 4.34 GLTTGAGASGRR88 pKa = 11.84 TGQRR92 pKa = 11.84 LSGSRR97 pKa = 11.84 EE98 pKa = 3.76 GRR100 pKa = 11.84 PHH102 pKa = 6.98 RR103 pKa = 11.84 GCGALLAHH111 pKa = 6.53 GPEE114 pKa = 4.59 VGGNTYY120 pKa = 9.52 PHH122 pKa = 7.25 LWSTHH127 pKa = 3.94 TSGPRR132 pKa = 11.84 ASLWANPSNKK142 pKa = 9.05 INPVIHH148 pKa = 5.92 YY149 pKa = 9.83 VYY151 pKa = 10.24 TDD153 pKa = 4.14 SINDD157 pKa = 3.1 NWALNLAKK165 pKa = 10.24 VYY167 pKa = 9.96 KK168 pKa = 10.2 IPVIHH173 pKa = 7.05 KK174 pKa = 8.75 PRR176 pKa = 11.84 SGPEE180 pKa = 3.5 TTYY183 pKa = 10.04 WPSGHH188 pKa = 6.52 IQTMPRR194 pKa = 11.84 YY195 pKa = 8.85 SKK197 pKa = 9.84 PIPMSINRR205 pKa = 11.84 RR206 pKa = 11.84 RR207 pKa = 11.84 AWQAAKK213 pKa = 10.37 DD214 pKa = 3.78 KK215 pKa = 10.99 RR216 pKa = 11.84 YY217 pKa = 9.57 IAYY220 pKa = 9.27 GKK222 pKa = 10.26 ARR224 pKa = 11.84 ATVVVPGYY232 pKa = 8.88 TRR234 pKa = 11.84 TGGTFGRR241 pKa = 11.84 FTGGPGSEE249 pKa = 3.75 NKK251 pKa = 9.95 FFDD254 pKa = 3.56 TALGFSFDD262 pKa = 3.76 STGEE266 pKa = 4.17 VPATGQLNLIPQGVTEE282 pKa = 4.17 STRR285 pKa = 11.84 VGRR288 pKa = 11.84 SCILKK293 pKa = 10.17 SIQIKK298 pKa = 10.21 GRR300 pKa = 11.84 LRR302 pKa = 11.84 FDD304 pKa = 3.67 PGAAADD310 pKa = 3.98 SSTIAYY316 pKa = 9.39 LYY318 pKa = 10.43 LVQDD322 pKa = 4.02 SQCNGAAAAVTDD334 pKa = 4.11 VLTSATMAGAIVNMDD349 pKa = 2.83 NSQRR353 pKa = 11.84 FRR355 pKa = 11.84 ILKK358 pKa = 9.02 KK359 pKa = 8.56 WTWALNSASGVSTAYY374 pKa = 9.34 NTVDD378 pKa = 3.82 RR379 pKa = 11.84 AIDD382 pKa = 3.78 YY383 pKa = 9.48 YY384 pKa = 10.47 RR385 pKa = 11.84 KK386 pKa = 9.79 CHH388 pKa = 6.23 IPLDD392 pKa = 4.27 FSSTTGAIGEE402 pKa = 4.12 IRR404 pKa = 11.84 SNNVFLLAGSADD416 pKa = 3.75 TDD418 pKa = 3.81 DD419 pKa = 5.19 LVAFSGVARR428 pKa = 11.84 VRR430 pKa = 11.84 FSDD433 pKa = 3.48 KK434 pKa = 11.17
Molecular weight: 47.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.273
IPC2_protein 9.531
IPC_protein 9.838
Toseland 10.043
ProMoST 9.853
Dawson 10.277
Bjellqvist 9.999
Wikipedia 10.482
Rodwell 10.555
Grimsley 10.379
Solomon 10.306
Lehninger 10.262
Nozaki 10.058
DTASelect 9.999
Thurlkill 10.116
EMBOSS 10.452
Sillero 10.204
Patrickios 9.809
IPC_peptide 10.306
IPC2_peptide 8.843
IPC2.peptide.svr19 8.577
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3
0
3
725
122
434
241.7
26.92
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.0 ± 0.885
1.103 ± 0.436
4.414 ± 0.804
4.414 ± 1.585
3.862 ± 0.16
8.414 ± 1.672
2.621 ± 0.339
6.069 ± 0.915
5.103 ± 0.859
6.207 ± 0.593
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.931 ± 0.816
4.0 ± 0.369
6.069 ± 1.107
3.034 ± 0.739
6.897 ± 0.412
9.655 ± 1.461
6.483 ± 0.976
4.966 ± 0.678
2.759 ± 1.036
4.0 ± 0.995
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here