Sporolactobacillus shoreae
Average proteome isoelectric point is 6.81
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3461 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Z0GQC2|A0A4Z0GQC2_9BACL LysR family transcriptional regulator OS=Sporolactobacillus shoreae OX=1465501 GN=E4665_06335 PE=3 SV=1
MM1 pKa = 7.52 EE2 pKa = 6.11 KK3 pKa = 11.05 YY4 pKa = 10.22 MIVDD8 pKa = 3.63 KK9 pKa = 8.22 EE10 pKa = 4.55 TCISCGACAASAPDD24 pKa = 4.23 LFDD27 pKa = 4.7 YY28 pKa = 11.2 DD29 pKa = 4.67 DD30 pKa = 5.68 DD31 pKa = 4.79 GLAFSLLDD39 pKa = 3.71 GNKK42 pKa = 10.16 GDD44 pKa = 3.58 VSVPDD49 pKa = 4.01 EE50 pKa = 4.47 LLEE53 pKa = 4.58 DD54 pKa = 4.28 LADD57 pKa = 4.86 AYY59 pKa = 10.58 DD60 pKa = 4.03 GCPTEE65 pKa = 4.88 SIRR68 pKa = 11.84 IGDD71 pKa = 3.87 QPFHH75 pKa = 6.77 GSPDD79 pKa = 3.35 KK80 pKa = 11.09 VAEE83 pKa = 4.07 NN84 pKa = 3.72
Molecular weight: 8.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.717
IPC2_protein 3.668
IPC_protein 3.656
Toseland 3.427
ProMoST 3.846
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.63
Rodwell 3.478
Grimsley 3.338
Solomon 3.643
Lehninger 3.605
Nozaki 3.783
DTASelect 4.037
Thurlkill 3.503
EMBOSS 3.643
Sillero 3.783
Patrickios 0.846
IPC_peptide 3.63
IPC2_peptide 3.745
IPC2.peptide.svr19 3.706
Protein with the highest isoelectric point:
>tr|A0A4Z0GQA0|A0A4Z0GQA0_9BACL Uncharacterized protein OS=Sporolactobacillus shoreae OX=1465501 GN=E4665_08440 PE=4 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.42 RR3 pKa = 11.84 TYY5 pKa = 10.33 QPNKK9 pKa = 8.47 RR10 pKa = 11.84 SRR12 pKa = 11.84 KK13 pKa = 7.94 KK14 pKa = 8.75 VHH16 pKa = 5.84 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATANGRR28 pKa = 11.84 KK29 pKa = 9.11 VLANRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.69 GRR39 pKa = 11.84 KK40 pKa = 8.69 VLSAA44 pKa = 4.05
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.414
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.384
Grimsley 12.661
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.106
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.021
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3461
0
3461
1011389
19
1595
292.2
32.69
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.536 ± 0.043
0.829 ± 0.013
5.299 ± 0.036
6.118 ± 0.044
4.594 ± 0.036
7.076 ± 0.036
2.216 ± 0.019
7.405 ± 0.038
6.361 ± 0.035
10.082 ± 0.048
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.667 ± 0.02
4.146 ± 0.032
3.795 ± 0.024
3.815 ± 0.028
4.834 ± 0.036
6.546 ± 0.036
5.359 ± 0.025
6.816 ± 0.031
1.076 ± 0.016
3.429 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here