Blastocystis sp. subtype 1 (strain ATCC 50177 / NandII)

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Bigyra; Opalozoa; Opalinata; Blastocystidae; Blastocystis; Blastocystis sp. subtypes; Blastocystis sp. subtype 1

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6377 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A196S7T1|A0A196S7T1_BLAHN Eukaryotic initiation factor 4A-III OS=Blastocystis sp. subtype 1 (strain ATCC 50177 / NandII) OX=478820 GN=AV274_6182 PE=4 SV=1
MM1 pKa = 7.41SVTEE5 pKa = 4.19STRR8 pKa = 11.84EE9 pKa = 3.94AGLDD13 pKa = 3.43LAKK16 pKa = 10.75NDD18 pKa = 4.44LLYY21 pKa = 10.78TFDD24 pKa = 4.68GVEE27 pKa = 4.15CMVNQAQSHH36 pKa = 5.0SAGTLYY42 pKa = 9.55LTKK45 pKa = 10.87DD46 pKa = 3.28NLVFVAPEE54 pKa = 4.26GEE56 pKa = 4.43DD57 pKa = 3.37YY58 pKa = 11.33SLAIEE63 pKa = 4.18YY64 pKa = 8.79PAIGVHH70 pKa = 6.49AVCQEE75 pKa = 3.64DD76 pKa = 4.45TLWKK80 pKa = 10.11RR81 pKa = 11.84PCIYY85 pKa = 10.5CQICDD90 pKa = 4.03PQAEE94 pKa = 4.49DD95 pKa = 3.31SGEE98 pKa = 4.16VNVICGDD105 pKa = 3.67PSFDD109 pKa = 4.56AFDD112 pKa = 3.25QGEE115 pKa = 4.18EE116 pKa = 4.08VEE118 pKa = 4.33EE119 pKa = 4.89GEE121 pKa = 4.63EE122 pKa = 4.14EE123 pKa = 4.13EE124 pKa = 4.74DD125 pKa = 3.56DD126 pKa = 4.22GSIFEE131 pKa = 4.61VLFSPLEE138 pKa = 4.01TEE140 pKa = 5.03KK141 pKa = 10.47IDD143 pKa = 4.11SVFDD147 pKa = 4.46GISRR151 pKa = 11.84LQVMHH156 pKa = 7.48PDD158 pKa = 3.44PVKK161 pKa = 10.84EE162 pKa = 4.01EE163 pKa = 4.5EE164 pKa = 4.14EE165 pKa = 4.93DD166 pKa = 3.91NMFMTANGLVSVEE179 pKa = 4.2DD180 pKa = 4.65HH181 pKa = 6.32ICSLFEE187 pKa = 3.76KK188 pKa = 10.51AAAEE192 pKa = 4.3YY193 pKa = 10.19EE194 pKa = 4.63GKK196 pKa = 10.14DD197 pKa = 3.37HH198 pKa = 6.92EE199 pKa = 4.47

Molecular weight: 22.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A196SL45|A0A196SL45_BLAHN Drug/Metabolite Transporter (DMT) Superfamily OS=Blastocystis sp. subtype 1 (strain ATCC 50177 / NandII) OX=478820 GN=AV274_1270 PE=3 SV=1
MM1 pKa = 7.94PSQKK5 pKa = 10.37SFRR8 pKa = 11.84TKK10 pKa = 10.44RR11 pKa = 11.84ILAKK15 pKa = 10.37KK16 pKa = 8.59MRR18 pKa = 11.84QNRR21 pKa = 11.84PIPQWFRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.63RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.6WKK45 pKa = 8.07RR46 pKa = 11.84TKK48 pKa = 10.75LGII51 pKa = 3.98

Molecular weight: 6.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6377

0

6377

3227170

19

13458

506.1

56.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.595 ± 0.04

2.175 ± 0.017

5.257 ± 0.019

7.71 ± 0.05

4.231 ± 0.023

5.447 ± 0.031

2.089 ± 0.012

4.913 ± 0.027

6.006 ± 0.041

9.553 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.711 ± 0.014

4.018 ± 0.019

4.552 ± 0.028

3.625 ± 0.02

5.304 ± 0.025

7.493 ± 0.03

5.344 ± 0.023

7.52 ± 0.036

1.03 ± 0.009

3.398 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski