Amino acid dipepetide frequency for Blastocystis sp. subtype 1 (strain ATCC 50177 / NandII)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	7.014AlaAla: 7.014 ± 0.094	1.539AlaCys: 1.539 ± 0.026	3.35AlaAsp: 3.35 ± 0.037	5.471AlaGlu: 5.471 ± 0.075	3.581AlaPhe: 3.581 ± 0.045	3.816AlaGly: 3.816 ± 0.05	1.306AlaHis: 1.306 ± 0.025	3.858AlaIle: 3.858 ± 0.038	4.379AlaLys: 4.379 ± 0.062	7.69AlaLeu: 7.69 ± 0.072	2.223AlaMet: 2.223 ± 0.028	2.643AlaAsn: 2.643 ± 0.037	3.485AlaPro: 3.485 ± 0.059	2.719AlaGln: 2.719 ± 0.039	3.531AlaArg: 3.531 ± 0.038	5.926AlaSer: 5.926 ± 0.058	4.22AlaThr: 4.22 ± 0.05	6.151AlaVal: 6.151 ± 0.073	0.745AlaTrp: 0.745 ± 0.015	2.283AlaTyr: 2.283 ± 0.029	0.022AlaXaa: 0.022 ± 0.003
Cys	1.442CysAla: 1.442 ± 0.026	0.711CysCys: 0.711 ± 0.022	1.208CysAsp: 1.208 ± 0.023	1.277CysGlu: 1.277 ± 0.022	1.178CysPhe: 1.178 ± 0.021	1.467CysGly: 1.467 ± 0.025	0.466CysHis: 0.466 ± 0.015	1.179CysIle: 1.179 ± 0.02	0.946CysLys: 0.946 ± 0.019	2.104CysLeu: 2.104 ± 0.031	0.536CysMet: 0.536 ± 0.014	0.832CysAsn: 0.832 ± 0.017	1.027CysPro: 1.027 ± 0.03	0.635CysGln: 0.635 ± 0.018	1.059CysArg: 1.059 ± 0.022	1.679CysSer: 1.679 ± 0.03	1.066CysThr: 1.066 ± 0.022	1.848CysVal: 1.848 ± 0.029	0.213CysTrp: 0.213 ± 0.009	0.871CysTyr: 0.871 ± 0.029	0.002CysXaa: 0.002 ± 0.001
Asp	4.636AspAla: 4.636 ± 0.045	1.221AspCys: 1.221 ± 0.025	3.383AspAsp: 3.383 ± 0.043	4.002AspGlu: 4.002 ± 0.047	2.334AspPhe: 2.334 ± 0.028	3.26AspGly: 3.26 ± 0.04	0.997AspHis: 0.997 ± 0.017	2.737AspIle: 2.737 ± 0.033	2.591AspLys: 2.591 ± 0.035	4.644AspLeu: 4.644 ± 0.046	1.558AspMet: 1.558 ± 0.026	1.917AspAsn: 1.917 ± 0.027	2.168AspPro: 2.168 ± 0.035	1.218AspGln: 1.218 ± 0.018	2.469AspArg: 2.469 ± 0.031	3.921AspSer: 3.921 ± 0.047	2.433AspThr: 2.433 ± 0.032	4.272AspVal: 4.272 ± 0.045	0.65AspTrp: 0.65 ± 0.016	2.15AspTyr: 2.15 ± 0.029	0.009AspXaa: 0.009 ± 0.002
Glu	5.954GluAla: 5.954 ± 0.071	1.336GluCys: 1.336 ± 0.023	4.135GluAsp: 4.135 ± 0.042	10.65GluGlu: 10.65 ± 0.239	2.2GluPhe: 2.2 ± 0.03	4.429GluGly: 4.429 ± 0.042	1.223GluHis: 1.223 ± 0.02	3.048GluIle: 3.048 ± 0.036	6.944GluLys: 6.944 ± 0.127	6.463GluLeu: 6.463 ± 0.079	2.388GluMet: 2.388 ± 0.036	3.051GluAsn: 3.051 ± 0.038	2.218GluPro: 2.218 ± 0.041	2.508GluGln: 2.508 ± 0.035	3.841GluArg: 3.841 ± 0.058	4.481GluSer: 4.481 ± 0.05	3.37GluThr: 3.37 ± 0.035	5.545GluVal: 5.545 ± 0.064	0.888GluTrp: 0.888 ± 0.021	2.417GluTyr: 2.417 ± 0.032	0.015GluXaa: 0.015 ± 0.003
Phe	3.164PheAla: 3.164 ± 0.042	1.019PheCys: 1.019 ± 0.019	2.618PheAsp: 2.618 ± 0.032	2.307PheGlu: 2.307 ± 0.027	2.263PhePhe: 2.263 ± 0.035	2.435PheGly: 2.435 ± 0.051	0.993PheHis: 0.993 ± 0.021	2.13PheIle: 2.13 ± 0.031	1.793PheLys: 1.793 ± 0.027	4.008PheLeu: 4.008 ± 0.045	1.007PheMet: 1.007 ± 0.02	1.779PheAsn: 1.779 ± 0.028	1.88PhePro: 1.88 ± 0.029	1.437PheGln: 1.437 ± 0.024	1.941PheArg: 1.941 ± 0.026	3.769PheSer: 3.769 ± 0.044	2.432PheThr: 2.432 ± 0.037	3.343PheVal: 3.343 ± 0.045	0.401PheTrp: 0.401 ± 0.013	1.581PheTyr: 1.581 ± 0.025	0.011PheXaa: 0.011 ± 0.002
Gly	3.741GlyAla: 3.741 ± 0.055	1.31GlyCys: 1.31 ± 0.023	3.123GlyAsp: 3.123 ± 0.036	4.329GlyGlu: 4.329 ± 0.045	2.418GlyPhe: 2.418 ± 0.041	3.507GlyGly: 3.507 ± 0.063	1.016GlyHis: 1.016 ± 0.021	2.964GlyIle: 2.964 ± 0.034	3.61GlyLys: 3.61 ± 0.047	4.494GlyLeu: 4.494 ± 0.044	1.64GlyMet: 1.64 ± 0.03	2.292GlyAsn: 2.292 ± 0.036	1.294GlyPro: 1.294 ± 0.026	1.556GlyGln: 1.556 ± 0.027	2.821GlyArg: 2.821 ± 0.038	3.991GlySer: 3.991 ± 0.042	2.774GlyThr: 2.774 ± 0.032	4.81GlyVal: 4.81 ± 0.052	0.641GlyTrp: 0.641 ± 0.015	2.12GlyTyr: 2.12 ± 0.03	0.015GlyXaa: 0.015 ± 0.003
His	1.475HisAla: 1.475 ± 0.023	0.576HisCys: 0.576 ± 0.013	1.009HisAsp: 1.009 ± 0.02	1.023HisGlu: 1.023 ± 0.022	1.156HisPhe: 1.156 ± 0.018	1.112HisGly: 1.112 ± 0.024	0.626HisHis: 0.626 ± 0.019	1.204HisIle: 1.204 ± 0.023	0.903HisLys: 0.903 ± 0.017	2.024HisLeu: 2.024 ± 0.029	0.555HisMet: 0.555 ± 0.013	0.838HisAsn: 0.838 ± 0.014	1.248HisPro: 1.248 ± 0.023	0.636HisGln: 0.636 ± 0.014	1.152HisArg: 1.152 ± 0.02	1.655HisSer: 1.655 ± 0.025	1.156HisThr: 1.156 ± 0.021	1.464HisVal: 1.464 ± 0.024	0.216HisTrp: 0.216 ± 0.008	0.859HisTyr: 0.859 ± 0.017	0.004HisXaa: 0.004 ± 0.001
Ile	3.915IleAla: 3.915 ± 0.039	1.064IleCys: 1.064 ± 0.022	2.986IleAsp: 2.986 ± 0.035	3.099IleGlu: 3.099 ± 0.039	1.525IlePhe: 1.525 ± 0.024	2.655IleGly: 2.655 ± 0.041	1.32IleHis: 1.32 ± 0.021	2.532IleIle: 2.532 ± 0.036	2.195IleLys: 2.195 ± 0.029	4.603IleLeu: 4.603 ± 0.046	1.145IleMet: 1.145 ± 0.024	1.914IleAsn: 1.914 ± 0.03	2.924IlePro: 2.924 ± 0.038	2.09IleGln: 2.09 ± 0.028	2.914IleArg: 2.914 ± 0.041	3.523IleSer: 3.523 ± 0.04	3.1IleThr: 3.1 ± 0.036	3.78IleVal: 3.78 ± 0.04	0.431IleTrp: 0.431 ± 0.011	1.403IleTyr: 1.403 ± 0.023	0.008IleXaa: 0.008 ± 0.002
Lys	4.291LysAla: 4.291 ± 0.058	1.11LysCys: 1.11 ± 0.026	3.199LysAsp: 3.199 ± 0.038	7.276LysGlu: 7.276 ± 0.128	1.264LysPhe: 1.264 ± 0.026	3.191LysGly: 3.191 ± 0.04	1.102LysHis: 1.102 ± 0.021	2.062LysIle: 2.062 ± 0.03	6.097LysLys: 6.097 ± 0.118	4.296LysLeu: 4.296 ± 0.044	1.645LysMet: 1.645 ± 0.024	2.637LysAsn: 2.637 ± 0.035	2.779LysPro: 2.779 ± 0.057	2.515LysGln: 2.515 ± 0.033	3.768LysArg: 3.768 ± 0.056	3.545LysSer: 3.545 ± 0.048	3.091LysThr: 3.091 ± 0.038	3.595LysVal: 3.595 ± 0.044	0.661LysTrp: 0.661 ± 0.014	1.923LysTyr: 1.923 ± 0.026	0.01LysXaa: 0.01 ± 0.002
Leu	6.51LeuAla: 6.51 ± 0.058	2.197LeuCys: 2.197 ± 0.03	4.447LeuAsp: 4.447 ± 0.037	5.962LeuGlu: 5.962 ± 0.071	4.819LeuPhe: 4.819 ± 0.05	4.315LeuGly: 4.315 ± 0.037	2.471LeuHis: 2.471 ± 0.038	4.034LeuIle: 4.034 ± 0.042	5.549LeuLys: 5.549 ± 0.062	11.053LeuLeu: 11.053 ± 0.141	2.261LeuMet: 2.261 ± 0.027	3.776LeuAsn: 3.776 ± 0.042	5.192LeuPro: 5.192 ± 0.058	4.343LeuGln: 4.343 ± 0.052	5.809LeuArg: 5.809 ± 0.046	7.807LeuSer: 7.807 ± 0.063	4.896LeuThr: 4.896 ± 0.056	6.025LeuVal: 6.025 ± 0.056	0.913LeuTrp: 0.913 ± 0.02	3.125LeuTyr: 3.125 ± 0.037	0.021LeuXaa: 0.021 ± 0.003
Met	1.789MetAla: 1.789 ± 0.028	0.482MetCys: 0.482 ± 0.013	1.629MetAsp: 1.629 ± 0.022	2.355MetGlu: 2.355 ± 0.036	0.794MetPhe: 0.794 ± 0.019	1.54MetGly: 1.54 ± 0.027	0.493MetHis: 0.493 ± 0.012	1.332MetIle: 1.332 ± 0.021	2.242MetLys: 2.242 ± 0.037	2.204MetLeu: 2.204 ± 0.031	0.929MetMet: 0.929 ± 0.024	1.479MetAsn: 1.479 ± 0.022	1.224MetPro: 1.224 ± 0.026	1.12MetGln: 1.12 ± 0.024	1.48MetArg: 1.48 ± 0.023	1.901MetSer: 1.901 ± 0.025	1.461MetThr: 1.461 ± 0.026	1.661MetVal: 1.661 ± 0.03	0.244MetTrp: 0.244 ± 0.01	0.747MetTyr: 0.747 ± 0.019	0.006MetXaa: 0.006 ± 0.002
Asn	3.342AsnAla: 3.342 ± 0.035	0.925AsnCys: 0.925 ± 0.019	2.26AsnAsp: 2.26 ± 0.028	2.925AsnGlu: 2.925 ± 0.045	1.101AsnPhe: 1.101 ± 0.02	2.917AsnGly: 2.917 ± 0.043	0.943AsnHis: 0.943 ± 0.019	1.887AsnIle: 1.887 ± 0.03	2.017AsnLys: 2.017 ± 0.028	3.244AsnLeu: 3.244 ± 0.036	1.112AsnMet: 1.112 ± 0.022	1.733AsnAsn: 1.733 ± 0.042	2.216AsnPro: 2.216 ± 0.033	1.437AsnGln: 1.437 ± 0.023	2.206AsnArg: 2.206 ± 0.027	2.722AsnSer: 2.722 ± 0.034	2.428AsnThr: 2.428 ± 0.032	2.81AsnVal: 2.81 ± 0.037	0.47AsnTrp: 0.47 ± 0.014	1.482AsnTyr: 1.482 ± 0.026	0.007AsnXaa: 0.007 ± 0.001
Pro	4.592ProAla: 4.592 ± 0.07	0.751ProCys: 0.751 ± 0.018	1.962ProAsp: 1.962 ± 0.029	3.315ProGlu: 3.315 ± 0.034	2.171ProPhe: 2.171 ± 0.028	1.839ProGly: 1.839 ± 0.032	0.901ProHis: 0.901 ± 0.018	2.148ProIle: 2.148 ± 0.031	2.264ProLys: 2.264 ± 0.035	4.099ProLeu: 4.099 ± 0.05	1.121ProMet: 1.121 ± 0.025	1.624ProAsn: 1.624 ± 0.024	2.335ProPro: 2.335 ± 0.048	1.651ProGln: 1.651 ± 0.03	2.096ProArg: 2.096 ± 0.034	4.29ProSer: 4.29 ± 0.046	2.896ProThr: 2.896 ± 0.069	3.651ProVal: 3.651 ± 0.049	0.359ProTrp: 0.359 ± 0.013	1.44ProTyr: 1.44 ± 0.027	0.018ProXaa: 0.018 ± 0.003
Gln	2.42GlnAla: 2.42 ± 0.033	0.799GlnCys: 0.799 ± 0.017	1.36GlnAsp: 1.36 ± 0.02	2.559GlnGlu: 2.559 ± 0.037	1.589GlnPhe: 1.589 ± 0.024	1.703GlnGly: 1.703 ± 0.028	0.814GlnHis: 0.814 ± 0.016	1.708GlnIle: 1.708 ± 0.025	2.252GlnLys: 2.252 ± 0.03	3.854GlnLeu: 3.854 ± 0.045	0.984GlnMet: 0.984 ± 0.019	1.462GlnAsn: 1.462 ± 0.024	2.003GlnPro: 2.003 ± 0.049	1.974GlnGln: 1.974 ± 0.04	2.208GlnArg: 2.208 ± 0.032	2.751GlnSer: 2.751 ± 0.032	1.891GlnThr: 1.891 ± 0.028	1.994GlnVal: 1.994 ± 0.024	0.46GlnTrp: 0.46 ± 0.013	1.462GlnTyr: 1.462 ± 0.023	0.006GlnXaa: 0.006 ± 0.001
Arg	3.621ArgAla: 3.621 ± 0.047	1.268ArgCys: 1.268 ± 0.025	2.877ArgAsp: 2.877 ± 0.036	4.155ArgGlu: 4.155 ± 0.051	2.402ArgPhe: 2.402 ± 0.029	2.883ArgGly: 2.883 ± 0.036	1.089ArgHis: 1.089 ± 0.02	2.846ArgIle: 2.846 ± 0.035	3.158ArgLys: 3.158 ± 0.048	5.187ArgLeu: 5.187 ± 0.053	1.43ArgMet: 1.43 ± 0.024	2.206ArgAsn: 2.206 ± 0.028	1.715ArgPro: 1.715 ± 0.037	1.925ArgGln: 1.925 ± 0.026	3.545ArgArg: 3.545 ± 0.052	3.644ArgSer: 3.644 ± 0.039	2.329ArgThr: 2.329 ± 0.032	4.398ArgVal: 4.398 ± 0.047	0.59ArgTrp: 0.59 ± 0.014	1.757ArgTyr: 1.757 ± 0.024	0.011ArgXaa: 0.011 ± 0.002
Ser	5.32SerAla: 5.32 ± 0.054	1.549SerCys: 1.549 ± 0.026	3.963SerAsp: 3.963 ± 0.045	4.626SerGlu: 4.626 ± 0.054	4.115SerPhe: 4.115 ± 0.046	4.095SerGly: 4.095 ± 0.05	1.558SerHis: 1.558 ± 0.028	4.373SerIle: 4.373 ± 0.05	3.382SerLys: 3.382 ± 0.032	8.291SerLeu: 8.291 ± 0.08	1.817SerMet: 1.817 ± 0.027	2.738SerAsn: 2.738 ± 0.034	3.272SerPro: 3.272 ± 0.051	2.571SerGln: 2.571 ± 0.036	3.467SerArg: 3.467 ± 0.038	6.677SerSer: 6.677 ± 0.081	3.977SerThr: 3.977 ± 0.042	5.985SerVal: 5.985 ± 0.051	0.656SerTrp: 0.656 ± 0.014	2.481SerTyr: 2.481 ± 0.034	0.018SerXaa: 0.018 ± 0.003
Thr	4.257ThrAla: 4.257 ± 0.052	0.99ThrCys: 0.99 ± 0.021	2.4ThrAsp: 2.4 ± 0.029	3.427ThrGlu: 3.427 ± 0.039	1.982ThrPhe: 1.982 ± 0.036	3.023ThrGly: 3.023 ± 0.045	1.163ThrHis: 1.163 ± 0.022	3.084ThrIle: 3.084 ± 0.042	2.6ThrLys: 2.6 ± 0.03	5.633ThrLeu: 5.633 ± 0.054	1.334ThrMet: 1.334 ± 0.02	1.978ThrAsn: 1.978 ± 0.029	3.405ThrPro: 3.405 ± 0.061	1.943ThrGln: 1.943 ± 0.028	2.483ThrArg: 2.483 ± 0.029	3.745ThrSer: 3.745 ± 0.04	3.482ThrThr: 3.482 ± 0.053	4.495ThrVal: 4.495 ± 0.065	0.483ThrTrp: 0.483 ± 0.013	1.519ThrTyr: 1.519 ± 0.026	0.011ThrXaa: 0.011 ± 0.002
Val	5.463ValAla: 5.463 ± 0.066	1.687ValCys: 1.687 ± 0.029	4.014ValAsp: 4.014 ± 0.038	5.614ValGlu: 5.614 ± 0.063	3.533ValPhe: 3.533 ± 0.038	3.625ValGly: 3.625 ± 0.045	1.38ValHis: 1.38 ± 0.024	3.862ValIle: 3.862 ± 0.041	4.682ValLys: 4.682 ± 0.062	7.496ValLeu: 7.496 ± 0.068	2.252ValMet: 2.252 ± 0.028	3.135ValAsn: 3.135 ± 0.036	3.238ValPro: 3.238 ± 0.038	2.482ValGln: 2.482 ± 0.028	3.683ValArg: 3.683 ± 0.041	5.394ValSer: 5.394 ± 0.051	3.959ValThr: 3.959 ± 0.05	6.463ValVal: 6.463 ± 0.07	0.743ValTrp: 0.743 ± 0.018	2.482ValTyr: 2.482 ± 0.031	0.017ValXaa: 0.017 ± 0.003
Trp	0.523TrpAla: 0.523 ± 0.014	0.267TrpCys: 0.267 ± 0.01	0.612TrpAsp: 0.612 ± 0.014	0.687TrpGlu: 0.687 ± 0.015	0.422TrpPhe: 0.422 ± 0.011	0.6TrpGly: 0.6 ± 0.015	0.16TrpHis: 0.16 ± 0.008	0.514TrpIle: 0.514 ± 0.014	0.816TrpLys: 0.816 ± 0.015	0.905TrpLeu: 0.905 ± 0.02	0.321TrpMet: 0.321 ± 0.011	0.602TrpAsn: 0.602 ± 0.014	0.32TrpPro: 0.32 ± 0.01	0.329TrpGln: 0.329 ± 0.011	0.665TrpArg: 0.665 ± 0.016	0.872TrpSer: 0.872 ± 0.018	0.567TrpThr: 0.567 ± 0.015	0.648TrpVal: 0.648 ± 0.013	0.136TrpTrp: 0.136 ± 0.006	0.336TrpTyr: 0.336 ± 0.011	0.003TrpXaa: 0.003 ± 0.001
Tyr	2.464TyrAla: 2.464 ± 0.032	0.941TyrCys: 0.941 ± 0.019	2.027TyrAsp: 2.027 ± 0.03	2.02TyrGlu: 2.02 ± 0.029	1.45TyrPhe: 1.45 ± 0.026	2.041TyrGly: 2.041 ± 0.033	0.867TyrHis: 0.867 ± 0.018	1.612TyrIle: 1.612 ± 0.024	1.626TyrLys: 1.626 ± 0.027	3.217TyrLeu: 3.217 ± 0.039	0.85TyrMet: 0.85 ± 0.016	1.54TyrAsn: 1.54 ± 0.021	1.567TyrPro: 1.567 ± 0.027	1.156TyrGln: 1.156 ± 0.022	1.888TyrArg: 1.888 ± 0.027	2.626TyrSer: 2.626 ± 0.031	1.895TyrThr: 1.895 ± 0.029	2.248TyrVal: 2.248 ± 0.032	0.401TyrTrp: 0.401 ± 0.01	1.54TyrTyr: 1.54 ± 0.027	0.007TyrXaa: 0.007 ± 0.002
Xaa	0.021XaaAla: 0.021 ± 0.003	0.003XaaCys: 0.003 ± 0.001	0.01XaaAsp: 0.01 ± 0.002	0.024XaaGlu: 0.024 ± 0.004	0.011XaaPhe: 0.011 ± 0.002	0.014XaaGly: 0.014 ± 0.002	0.002XaaHis: 0.002 ± 0.001	0.011XaaIle: 0.011 ± 0.002	0.011XaaLys: 0.011 ± 0.002	0.019XaaLeu: 0.019 ± 0.003	0.005XaaMet: 0.005 ± 0.001	0.009XaaAsn: 0.009 ± 0.002	0.014XaaPro: 0.014 ± 0.003	0.005XaaGln: 0.005 ± 0.001	0.012XaaArg: 0.012 ± 0.002	0.014XaaSer: 0.014 ± 0.003	0.013XaaThr: 0.013 ± 0.002	0.017XaaVal: 0.017 ± 0.003	0.004XaaTrp: 0.004 ± 0.001	0.005XaaTyr: 0.005 ± 0.001	0.051XaaXaa: 0.051 ± 0.006

Statistics based on 6377 proteins (3227170 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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