Haloechinothrix alba
Average proteome isoelectric point is 5.96
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4481 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A238WMW8|A0A238WMW8_9PSEU Uncharacterized protein OS=Haloechinothrix alba OX=664784 GN=SAMN06265360_1076 PE=4 SV=1
MM1 pKa = 7.55 RR2 pKa = 11.84 LVSRR6 pKa = 11.84 RR7 pKa = 11.84 VVAVLASGALATFAFAAPASASGYY31 pKa = 9.88 GADD34 pKa = 4.4 DD35 pKa = 4.14 AGGAAINIVADD46 pKa = 4.05 TVSAAQEE53 pKa = 4.27 VTGSWSAEE61 pKa = 3.64 RR62 pKa = 11.84 MRR64 pKa = 11.84 QAVPMEE70 pKa = 3.97 QLVSEE75 pKa = 4.48 SGDD78 pKa = 3.54 VISLGEE84 pKa = 4.18 VVGSLTGGDD93 pKa = 3.83 PVSIPATKK101 pKa = 10.44 RR102 pKa = 11.84 LDD104 pKa = 3.68 LGSGLGGVLGSASGEE119 pKa = 4.0 PWTGGGEE126 pKa = 4.31 VASTAGRR133 pKa = 11.84 VFFTVDD139 pKa = 3.35 GQDD142 pKa = 3.43 ASCSGNAVTSEE153 pKa = 4.05 NNSTVITAGHH163 pKa = 5.94 CVRR166 pKa = 11.84 MGGEE170 pKa = 3.71 WHH172 pKa = 7.42 DD173 pKa = 3.81 DD174 pKa = 3.01 WVFVPGYY181 pKa = 10.59 DD182 pKa = 4.9 NGDD185 pKa = 3.3 APYY188 pKa = 10.78 GEE190 pKa = 4.75 WPAEE194 pKa = 3.97 TTMATQEE201 pKa = 4.12 WVEE204 pKa = 4.38 SEE206 pKa = 4.56 DD207 pKa = 3.23 INYY210 pKa = 10.33 DD211 pKa = 2.93 VGAAVVHH218 pKa = 5.74 EE219 pKa = 4.3 QDD221 pKa = 3.62 GQSLTEE227 pKa = 3.95 VVGGQGIAFNQEE239 pKa = 3.58 RR240 pKa = 11.84 GQPMYY245 pKa = 11.27 SFGYY249 pKa = 8.94 PAASPYY255 pKa = 10.71 DD256 pKa = 3.78 GSEE259 pKa = 4.75 LIHH262 pKa = 6.85 CSGDD266 pKa = 3.53 TFDD269 pKa = 6.19 DD270 pKa = 4.16 PLFSDD275 pKa = 5.21 AMGMDD280 pKa = 4.41 CDD282 pKa = 3.9 MTGGSSGGPWFLDD295 pKa = 3.29 FDD297 pKa = 4.27 EE298 pKa = 4.79 QSGTGLQSSVNSFGYY313 pKa = 10.87 AFLPGTMFGPYY324 pKa = 9.89 FGDD327 pKa = 3.57 EE328 pKa = 3.94 AQEE331 pKa = 4.22 VYY333 pKa = 10.69 EE334 pKa = 4.3 RR335 pKa = 11.84 ASTFF339 pKa = 3.1
Molecular weight: 35.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.716
IPC2_protein 3.77
IPC_protein 3.77
Toseland 3.554
ProMoST 3.923
Dawson 3.745
Bjellqvist 3.897
Wikipedia 3.668
Rodwell 3.592
Grimsley 3.465
Solomon 3.745
Lehninger 3.694
Nozaki 3.859
DTASelect 4.075
Thurlkill 3.605
EMBOSS 3.681
Sillero 3.884
Patrickios 0.896
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.778
Protein with the highest isoelectric point:
>tr|A0A239AWC3|A0A239AWC3_9PSEU Integrase core domain-containing protein (Fragment) OS=Haloechinothrix alba OX=664784 GN=SAMN06265360_1614 PE=4 SV=1
MM1 pKa = 7.85 RR2 pKa = 11.84 FARR5 pKa = 11.84 PKK7 pKa = 10.63 AGVALGRR14 pKa = 11.84 GLGALGRR21 pKa = 11.84 WVRR24 pKa = 11.84 RR25 pKa = 11.84 VRR27 pKa = 11.84 LPRR30 pKa = 11.84 VRR32 pKa = 11.84 VGVLWGWLRR41 pKa = 11.84 RR42 pKa = 11.84 VRR44 pKa = 11.84 VSGRR48 pKa = 11.84 GLAAVAA54 pKa = 4.27
Molecular weight: 5.99 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.301
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.554
Rodwell 12.618
Grimsley 13.115
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.34
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.251
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4481
0
4481
1395595
25
2307
311.4
33.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.665 ± 0.052
0.847 ± 0.01
6.395 ± 0.029
6.569 ± 0.035
2.697 ± 0.02
9.335 ± 0.033
2.407 ± 0.017
3.519 ± 0.022
1.784 ± 0.024
9.821 ± 0.043
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.888 ± 0.016
1.867 ± 0.016
5.544 ± 0.031
2.873 ± 0.021
8.064 ± 0.033
5.545 ± 0.024
5.972 ± 0.023
8.743 ± 0.034
1.407 ± 0.016
2.058 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here