Ralstonia phage RS-PI-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Okabevirinae; Ampunavirus; Ralstonia virus RSPI1

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S6L1A4|A0A1S6L1A4_9CAUD Uncharacterized protein OS=Ralstonia phage RS-PI-1 OX=1958965 PE=4 SV=1
MM1 pKa = 7.66GGPAMKK7 pKa = 10.11YY8 pKa = 10.09VYY10 pKa = 10.99ADD12 pKa = 3.4LCVDD16 pKa = 3.47EE17 pKa = 4.89NGEE20 pKa = 4.24YY21 pKa = 10.38VAVPVEE27 pKa = 4.11EE28 pKa = 4.54LPEE31 pKa = 4.11NVFATVPVADD41 pKa = 4.26HH42 pKa = 6.61VLAVWTWEE50 pKa = 4.1DD51 pKa = 3.42VPRR54 pKa = 11.84AA55 pKa = 3.64

Molecular weight: 6.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S6L1E2|A0A1S6L1E2_9CAUD Uncharacterized protein OS=Ralstonia phage RS-PI-1 OX=1958965 PE=4 SV=1
MM1 pKa = 7.3NRR3 pKa = 11.84FEE5 pKa = 4.3NPIVQRR11 pKa = 11.84TLTPVGGTRR20 pKa = 11.84PGKK23 pKa = 10.79AMTRR27 pKa = 11.84KK28 pKa = 9.5KK29 pKa = 10.4DD30 pKa = 3.31RR31 pKa = 11.84KK32 pKa = 9.01ARR34 pKa = 11.84KK35 pKa = 9.16LGFVNWQHH43 pKa = 7.15FISTVMANPALAANIHH59 pKa = 6.71RR60 pKa = 11.84IAAAITARR68 pKa = 11.84RR69 pKa = 11.84LGAQQ73 pKa = 3.14

Molecular weight: 8.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

13124

43

1686

273.4

30.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.33 ± 0.599

0.678 ± 0.118

6.423 ± 0.245

5.867 ± 0.393

3.52 ± 0.162

8.153 ± 0.349

1.874 ± 0.153

4.366 ± 0.185

5.174 ± 0.388

7.947 ± 0.344

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.347 ± 0.129

4.518 ± 0.296

4.252 ± 0.169

4.351 ± 0.358

6.096 ± 0.342

5.646 ± 0.247

5.623 ± 0.196

7.101 ± 0.256

1.524 ± 0.122

3.208 ± 0.185
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski