Amino acid dipepetide frequency for Ralstonia phage RS-PI-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.46AlaAla: 16.46 ± 1.74
0.914AlaCys: 0.914 ± 0.253
6.477AlaAsp: 6.477 ± 0.943
6.858AlaGlu: 6.858 ± 0.846
2.972AlaPhe: 2.972 ± 0.363
8.458AlaGly: 8.458 ± 1.041
1.981AlaHis: 1.981 ± 0.456
4.648AlaIle: 4.648 ± 0.587
4.877AlaLys: 4.877 ± 0.71
10.897AlaLeu: 10.897 ± 1.246
3.2AlaMet: 3.2 ± 0.485
5.639AlaAsn: 5.639 ± 1.194
4.115AlaPro: 4.115 ± 0.55
4.572AlaGln: 4.572 ± 0.95
6.63AlaArg: 6.63 ± 0.775
7.011AlaSer: 7.011 ± 0.893
4.801AlaThr: 4.801 ± 0.687
6.782AlaVal: 6.782 ± 0.797
1.905AlaTrp: 1.905 ± 0.38
4.039AlaTyr: 4.039 ± 0.448
0.0AlaXaa: 0.0 ± 0.0
Cys
0.686CysAla: 0.686 ± 0.3
0.0CysCys: 0.0 ± 0.0
0.457CysAsp: 0.457 ± 0.22
0.381CysGlu: 0.381 ± 0.174
0.229CysPhe: 0.229 ± 0.134
0.533CysGly: 0.533 ± 0.291
0.152CysHis: 0.152 ± 0.11
0.305CysIle: 0.305 ± 0.161
0.229CysLys: 0.229 ± 0.148
0.838CysLeu: 0.838 ± 0.269
0.381CysMet: 0.381 ± 0.185
0.457CysAsn: 0.457 ± 0.183
0.152CysPro: 0.152 ± 0.121
0.305CysGln: 0.305 ± 0.166
0.152CysArg: 0.152 ± 0.111
0.229CysSer: 0.229 ± 0.128
0.381CysThr: 0.381 ± 0.201
0.762CysVal: 0.762 ± 0.256
0.0CysTrp: 0.0 ± 0.0
0.152CysTyr: 0.152 ± 0.105
0.0CysXaa: 0.0 ± 0.0
Asp
8.763AspAla: 8.763 ± 1.025
0.229AspCys: 0.229 ± 0.149
5.258AspAsp: 5.258 ± 1.104
3.353AspGlu: 3.353 ± 0.577
2.896AspPhe: 2.896 ± 0.494
5.182AspGly: 5.182 ± 0.757
1.219AspHis: 1.219 ± 0.259
3.581AspIle: 3.581 ± 0.532
2.667AspLys: 2.667 ± 0.382
4.42AspLeu: 4.42 ± 0.608
1.829AspMet: 1.829 ± 0.338
2.057AspAsn: 2.057 ± 0.419
4.496AspPro: 4.496 ± 0.503
2.362AspGln: 2.362 ± 0.426
3.963AspArg: 3.963 ± 0.466
1.981AspSer: 1.981 ± 0.363
3.429AspThr: 3.429 ± 0.441
3.2AspVal: 3.2 ± 0.538
1.143AspTrp: 1.143 ± 0.311
2.21AspTyr: 2.21 ± 0.363
0.0AspXaa: 0.0 ± 0.0
Glu
5.715GluAla: 5.715 ± 0.742
0.533GluCys: 0.533 ± 0.191
4.115GluAsp: 4.115 ± 0.558
3.81GluGlu: 3.81 ± 0.643
2.21GluPhe: 2.21 ± 0.363
3.81GluGly: 3.81 ± 0.441
1.372GluHis: 1.372 ± 0.287
1.829GluIle: 1.829 ± 0.441
3.2GluLys: 3.2 ± 0.674
6.172GluLeu: 6.172 ± 0.956
1.143GluMet: 1.143 ± 0.271
2.515GluAsn: 2.515 ± 0.498
1.981GluPro: 1.981 ± 0.471
3.963GluGln: 3.963 ± 0.407
4.115GluArg: 4.115 ± 0.803
2.515GluSer: 2.515 ± 0.595
2.362GluThr: 2.362 ± 0.291
4.267GluVal: 4.267 ± 0.855
1.067GluTrp: 1.067 ± 0.289
1.981GluTyr: 1.981 ± 0.381
0.0GluXaa: 0.0 ± 0.0
Phe
3.2PheAla: 3.2 ± 0.427
0.229PheCys: 0.229 ± 0.122
2.819PheAsp: 2.819 ± 0.619
2.819PheGlu: 2.819 ± 0.432
1.372PhePhe: 1.372 ± 0.357
2.972PheGly: 2.972 ± 0.57
0.991PheHis: 0.991 ± 0.216
1.905PheIle: 1.905 ± 0.332
1.448PheLys: 1.448 ± 0.387
1.981PheLeu: 1.981 ± 0.382
0.914PheMet: 0.914 ± 0.345
1.372PheAsn: 1.372 ± 0.383
1.219PhePro: 1.219 ± 0.301
1.448PheGln: 1.448 ± 0.248
1.753PheArg: 1.753 ± 0.359
2.896PheSer: 2.896 ± 0.532
1.829PheThr: 1.829 ± 0.368
2.667PheVal: 2.667 ± 0.431
0.229PheTrp: 0.229 ± 0.144
1.143PheTyr: 1.143 ± 0.257
0.0PheXaa: 0.0 ± 0.0
Gly
6.706GlyAla: 6.706 ± 0.921
0.305GlyCys: 0.305 ± 0.168
4.725GlyAsp: 4.725 ± 0.75
4.953GlyGlu: 4.953 ± 0.637
3.581GlyPhe: 3.581 ± 0.752
8.535GlyGly: 8.535 ± 1.101
1.524GlyHis: 1.524 ± 0.414
3.658GlyIle: 3.658 ± 0.499
4.267GlyLys: 4.267 ± 0.567
4.648GlyLeu: 4.648 ± 0.673
2.057GlyMet: 2.057 ± 0.468
3.963GlyAsn: 3.963 ± 0.606
2.667GlyPro: 2.667 ± 0.681
3.429GlyGln: 3.429 ± 0.564
4.877GlyArg: 4.877 ± 0.66
4.801GlySer: 4.801 ± 0.594
6.172GlyThr: 6.172 ± 0.8
6.249GlyVal: 6.249 ± 0.801
1.067GlyTrp: 1.067 ± 0.344
3.353GlyTyr: 3.353 ± 0.602
0.0GlyXaa: 0.0 ± 0.0
His
1.295HisAla: 1.295 ± 0.286
0.152HisCys: 0.152 ± 0.106
1.6HisAsp: 1.6 ± 0.362
1.524HisGlu: 1.524 ± 0.379
0.991HisPhe: 0.991 ± 0.207
1.676HisGly: 1.676 ± 0.268
0.381HisHis: 0.381 ± 0.159
0.991HisIle: 0.991 ± 0.318
0.61HisLys: 0.61 ± 0.228
0.991HisLeu: 0.991 ± 0.276
0.762HisMet: 0.762 ± 0.229
0.533HisAsn: 0.533 ± 0.198
0.686HisPro: 0.686 ± 0.226
0.686HisGln: 0.686 ± 0.221
1.067HisArg: 1.067 ± 0.378
0.838HisSer: 0.838 ± 0.246
1.219HisThr: 1.219 ± 0.424
2.21HisVal: 2.21 ± 0.413
0.0HisTrp: 0.0 ± 0.0
0.533HisTyr: 0.533 ± 0.182
0.0HisXaa: 0.0 ± 0.0
Ile
5.639IleAla: 5.639 ± 0.578
0.533IleCys: 0.533 ± 0.176
3.277IleAsp: 3.277 ± 0.447
3.81IleGlu: 3.81 ± 0.738
0.914IlePhe: 0.914 ± 0.283
4.42IleGly: 4.42 ± 0.573
0.838IleHis: 0.838 ± 0.249
1.829IleIle: 1.829 ± 0.49
2.286IleLys: 2.286 ± 0.471
2.438IleLeu: 2.438 ± 0.638
0.305IleMet: 0.305 ± 0.145
2.972IleAsn: 2.972 ± 0.669
1.295IlePro: 1.295 ± 0.294
1.676IleGln: 1.676 ± 0.367
2.515IleArg: 2.515 ± 0.617
2.134IleSer: 2.134 ± 0.388
2.438IleThr: 2.438 ± 0.563
2.667IleVal: 2.667 ± 0.444
0.305IleTrp: 0.305 ± 0.177
1.372IleTyr: 1.372 ± 0.406
0.0IleXaa: 0.0 ± 0.0
Lys
7.62LysAla: 7.62 ± 0.896
0.457LysCys: 0.457 ± 0.214
2.438LysAsp: 2.438 ± 0.438
3.2LysGlu: 3.2 ± 0.546
1.372LysPhe: 1.372 ± 0.317
3.353LysGly: 3.353 ± 0.733
1.067LysHis: 1.067 ± 0.303
1.6LysIle: 1.6 ± 0.345
3.353LysLys: 3.353 ± 0.739
5.106LysLeu: 5.106 ± 0.648
0.533LysMet: 0.533 ± 0.19
2.515LysAsn: 2.515 ± 0.402
1.372LysPro: 1.372 ± 0.469
2.134LysGln: 2.134 ± 0.393
3.353LysArg: 3.353 ± 0.61
2.286LysSer: 2.286 ± 0.327
2.896LysThr: 2.896 ± 0.66
3.505LysVal: 3.505 ± 0.578
1.067LysTrp: 1.067 ± 0.263
2.515LysTyr: 2.515 ± 0.435
0.0LysXaa: 0.0 ± 0.0
Leu
9.982LeuAla: 9.982 ± 1.022
0.61LeuCys: 0.61 ± 0.266
5.639LeuAsp: 5.639 ± 0.754
4.039LeuGlu: 4.039 ± 0.611
1.829LeuPhe: 1.829 ± 0.371
6.172LeuGly: 6.172 ± 0.659
1.524LeuHis: 1.524 ± 0.439
2.515LeuIle: 2.515 ± 0.501
4.496LeuLys: 4.496 ± 0.617
6.096LeuLeu: 6.096 ± 0.741
1.448LeuMet: 1.448 ± 0.34
3.734LeuAsn: 3.734 ± 0.6
3.277LeuPro: 3.277 ± 0.63
3.581LeuGln: 3.581 ± 0.442
5.563LeuArg: 5.563 ± 0.62
5.182LeuSer: 5.182 ± 0.499
5.258LeuThr: 5.258 ± 0.552
5.563LeuVal: 5.563 ± 0.583
0.686LeuTrp: 0.686 ± 0.247
2.286LeuTyr: 2.286 ± 0.42
0.0LeuXaa: 0.0 ± 0.0
Met
2.438MetAla: 2.438 ± 0.379
0.076MetCys: 0.076 ± 0.077
1.753MetAsp: 1.753 ± 0.447
1.143MetGlu: 1.143 ± 0.383
0.61MetPhe: 0.61 ± 0.21
2.515MetGly: 2.515 ± 0.419
0.305MetHis: 0.305 ± 0.152
0.838MetIle: 0.838 ± 0.2
0.991MetLys: 0.991 ± 0.241
2.362MetLeu: 2.362 ± 0.452
0.533MetMet: 0.533 ± 0.263
1.295MetAsn: 1.295 ± 0.283
0.838MetPro: 0.838 ± 0.249
1.143MetGln: 1.143 ± 0.341
1.143MetArg: 1.143 ± 0.354
2.21MetSer: 2.21 ± 0.461
1.981MetThr: 1.981 ± 0.478
0.762MetVal: 0.762 ± 0.265
0.229MetTrp: 0.229 ± 0.109
0.305MetTyr: 0.305 ± 0.135
0.0MetXaa: 0.0 ± 0.0
Asn
5.258AsnAla: 5.258 ± 1.132
0.457AsnCys: 0.457 ± 0.214
2.591AsnAsp: 2.591 ± 0.503
2.21AsnGlu: 2.21 ± 0.409
1.295AsnPhe: 1.295 ± 0.28
4.725AsnGly: 4.725 ± 0.75
0.762AsnHis: 0.762 ± 0.245
1.829AsnIle: 1.829 ± 0.492
1.905AsnLys: 1.905 ± 0.403
3.81AsnLeu: 3.81 ± 0.449
1.524AsnMet: 1.524 ± 0.289
1.753AsnAsn: 1.753 ± 0.348
3.81AsnPro: 3.81 ± 0.374
1.524AsnGln: 1.524 ± 0.557
2.362AsnArg: 2.362 ± 0.4
2.362AsnSer: 2.362 ± 0.739
3.505AsnThr: 3.505 ± 0.789
2.057AsnVal: 2.057 ± 0.465
0.61AsnTrp: 0.61 ± 0.183
0.838AsnTyr: 0.838 ± 0.241
0.0AsnXaa: 0.0 ± 0.0
Pro
4.496ProAla: 4.496 ± 0.989
0.229ProCys: 0.229 ± 0.148
3.2ProAsp: 3.2 ± 0.433
3.048ProGlu: 3.048 ± 0.526
1.524ProPhe: 1.524 ± 0.276
3.81ProGly: 3.81 ± 0.683
0.229ProHis: 0.229 ± 0.122
1.6ProIle: 1.6 ± 0.321
2.667ProLys: 2.667 ± 0.571
2.896ProLeu: 2.896 ± 0.561
1.067ProMet: 1.067 ± 0.311
1.676ProAsn: 1.676 ± 0.334
1.6ProPro: 1.6 ± 0.663
1.753ProGln: 1.753 ± 0.336
1.676ProArg: 1.676 ± 0.361
2.591ProSer: 2.591 ± 0.409
2.515ProThr: 2.515 ± 0.39
3.2ProVal: 3.2 ± 0.496
0.838ProTrp: 0.838 ± 0.227
1.905ProTyr: 1.905 ± 0.457
0.0ProXaa: 0.0 ± 0.0
Gln
6.096GlnAla: 6.096 ± 1.271
0.229GlnCys: 0.229 ± 0.15
1.829GlnAsp: 1.829 ± 0.411
1.372GlnGlu: 1.372 ± 0.317
1.753GlnPhe: 1.753 ± 0.331
2.743GlnGly: 2.743 ± 0.498
1.448GlnHis: 1.448 ± 0.368
1.295GlnIle: 1.295 ± 0.327
1.981GlnLys: 1.981 ± 0.428
4.191GlnLeu: 4.191 ± 0.552
1.219GlnMet: 1.219 ± 0.371
1.448GlnAsn: 1.448 ± 0.341
1.753GlnPro: 1.753 ± 0.373
2.515GlnGln: 2.515 ± 0.673
3.048GlnArg: 3.048 ± 0.592
3.581GlnSer: 3.581 ± 0.523
1.524GlnThr: 1.524 ± 0.367
3.81GlnVal: 3.81 ± 0.543
0.762GlnTrp: 0.762 ± 0.228
0.914GlnTyr: 0.914 ± 0.334
0.0GlnXaa: 0.0 ± 0.0
Arg
5.639ArgAla: 5.639 ± 0.512
0.229ArgCys: 0.229 ± 0.133
4.496ArgAsp: 4.496 ± 0.83
3.2ArgGlu: 3.2 ± 0.75
2.972ArgPhe: 2.972 ± 0.515
4.725ArgGly: 4.725 ± 0.601
1.067ArgHis: 1.067 ± 0.337
3.581ArgIle: 3.581 ± 0.641
3.505ArgLys: 3.505 ± 0.488
5.487ArgLeu: 5.487 ± 0.642
1.524ArgMet: 1.524 ± 0.473
2.286ArgAsn: 2.286 ± 0.473
2.743ArgPro: 2.743 ± 0.511
1.753ArgGln: 1.753 ± 0.392
3.963ArgArg: 3.963 ± 0.756
2.972ArgSer: 2.972 ± 0.447
2.972ArgThr: 2.972 ± 0.46
4.725ArgVal: 4.725 ± 0.652
1.448ArgTrp: 1.448 ± 0.315
1.676ArgTyr: 1.676 ± 0.367
0.0ArgXaa: 0.0 ± 0.0
Ser
6.02SerAla: 6.02 ± 0.821
0.381SerCys: 0.381 ± 0.204
3.429SerAsp: 3.429 ± 0.693
2.515SerGlu: 2.515 ± 0.382
1.829SerPhe: 1.829 ± 0.413
3.81SerGly: 3.81 ± 0.633
0.457SerHis: 0.457 ± 0.199
2.743SerIle: 2.743 ± 0.4
2.819SerLys: 2.819 ± 0.458
4.191SerLeu: 4.191 ± 0.637
1.295SerMet: 1.295 ± 0.259
3.277SerAsn: 3.277 ± 0.655
2.819SerPro: 2.819 ± 0.515
2.515SerGln: 2.515 ± 0.594
3.734SerArg: 3.734 ± 0.587
3.429SerSer: 3.429 ± 0.709
3.734SerThr: 3.734 ± 0.753
4.877SerVal: 4.877 ± 0.778
0.686SerTrp: 0.686 ± 0.189
1.905SerTyr: 1.905 ± 0.473
0.0SerXaa: 0.0 ± 0.0
Thr
6.172ThrAla: 6.172 ± 0.819
0.229ThrCys: 0.229 ± 0.196
2.896ThrAsp: 2.896 ± 0.706
3.886ThrGlu: 3.886 ± 0.567
1.829ThrPhe: 1.829 ± 0.477
4.496ThrGly: 4.496 ± 0.531
0.914ThrHis: 0.914 ± 0.285
2.896ThrIle: 2.896 ± 0.542
3.81ThrLys: 3.81 ± 0.553
3.658ThrLeu: 3.658 ± 0.449
1.448ThrMet: 1.448 ± 0.421
1.6ThrAsn: 1.6 ± 0.364
3.734ThrPro: 3.734 ± 0.628
2.286ThrGln: 2.286 ± 0.389
2.743ThrArg: 2.743 ± 0.372
3.048ThrSer: 3.048 ± 0.527
3.2ThrThr: 3.2 ± 0.479
4.42ThrVal: 4.42 ± 0.706
0.914ThrTrp: 0.914 ± 0.243
2.057ThrTyr: 2.057 ± 0.455
0.0ThrXaa: 0.0 ± 0.0
Val
6.096ValAla: 6.096 ± 0.695
0.457ValCys: 0.457 ± 0.188
4.496ValAsp: 4.496 ± 0.393
3.734ValGlu: 3.734 ± 0.692
3.048ValPhe: 3.048 ± 0.412
6.401ValGly: 6.401 ± 0.897
1.524ValHis: 1.524 ± 0.436
3.886ValIle: 3.886 ± 0.551
4.877ValLys: 4.877 ± 0.731
4.648ValLeu: 4.648 ± 0.54
1.295ValMet: 1.295 ± 0.342
3.581ValAsn: 3.581 ± 0.472
2.515ValPro: 2.515 ± 0.476
3.658ValGln: 3.658 ± 0.538
4.877ValArg: 4.877 ± 0.79
3.505ValSer: 3.505 ± 0.562
3.505ValThr: 3.505 ± 0.516
5.334ValVal: 5.334 ± 0.69
1.448ValTrp: 1.448 ± 0.311
2.134ValTyr: 2.134 ± 0.435
0.0ValXaa: 0.0 ± 0.0
Trp
1.829TrpAla: 1.829 ± 0.392
0.229TrpCys: 0.229 ± 0.129
0.762TrpAsp: 0.762 ± 0.306
1.143TrpGlu: 1.143 ± 0.251
0.838TrpPhe: 0.838 ± 0.278
0.61TrpGly: 0.61 ± 0.209
0.381TrpHis: 0.381 ± 0.139
0.762TrpIle: 0.762 ± 0.226
0.61TrpLys: 0.61 ± 0.233
2.057TrpLeu: 2.057 ± 0.514
0.305TrpMet: 0.305 ± 0.154
0.686TrpAsn: 0.686 ± 0.29
0.076TrpPro: 0.076 ± 0.078
0.762TrpGln: 0.762 ± 0.189
0.762TrpArg: 0.762 ± 0.223
1.067TrpSer: 1.067 ± 0.277
0.762TrpThr: 0.762 ± 0.218
1.067TrpVal: 1.067 ± 0.305
0.305TrpTrp: 0.305 ± 0.164
0.229TrpTyr: 0.229 ± 0.111
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.2TyrAla: 3.2 ± 0.864
0.305TyrCys: 0.305 ± 0.134
1.981TyrAsp: 1.981 ± 0.395
1.676TyrGlu: 1.676 ± 0.33
1.143TyrPhe: 1.143 ± 0.24
2.591TyrGly: 2.591 ± 0.382
0.61TyrHis: 0.61 ± 0.253
1.372TyrIle: 1.372 ± 0.246
1.143TyrLys: 1.143 ± 0.323
2.591TyrLeu: 2.591 ± 0.455
0.61TyrMet: 0.61 ± 0.231
1.829TyrAsn: 1.829 ± 0.291
1.372TyrPro: 1.372 ± 0.252
1.448TyrGln: 1.448 ± 0.274
2.667TyrArg: 2.667 ± 0.485
1.829TyrSer: 1.829 ± 0.452
1.753TyrThr: 1.753 ± 0.417
2.896TyrVal: 2.896 ± 0.444
0.533TyrTrp: 0.533 ± 0.214
0.533TyrTyr: 0.533 ± 0.173
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 48 proteins (13124 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski