Dehalogenimonas lykanthroporepellens (strain ATCC BAA-1523 / JCM 15061 / BL-DC-9)
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1619 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D8K447|D8K447_DEHLB Amidophosphoribosyltransferase OS=Dehalogenimonas lykanthroporepellens (strain ATCC BAA-1523 / JCM 15061 / BL-DC-9) OX=552811 GN=purF PE=3 SV=1
MM1 pKa = 7.49 KK2 pKa = 10.32 KK3 pKa = 9.73 HH4 pKa = 5.91 LRR6 pKa = 11.84 KK7 pKa = 10.22 LMMVGFVMVLAMSALVVGCDD27 pKa = 3.31 STTDD31 pKa = 3.68 EE32 pKa = 5.01 PDD34 pKa = 3.08 PTQSVNQKK42 pKa = 8.82 PGQGVTMSPAKK53 pKa = 8.82 PTWSTGWFQTEE64 pKa = 4.06 VFIKK68 pKa = 10.5 ALEE71 pKa = 3.87 EE72 pKa = 3.62 LGYY75 pKa = 10.72 NVEE78 pKa = 5.0 DD79 pKa = 6.08 AIALDD84 pKa = 4.05 NPIFYY89 pKa = 10.85 NSVANGDD96 pKa = 3.27 VDD98 pKa = 4.59 FWADD102 pKa = 2.8 GWFPLHH108 pKa = 6.1 NQYY111 pKa = 11.24 LPIIEE116 pKa = 4.59 GKK118 pKa = 10.55 AEE120 pKa = 4.19 VVGEE124 pKa = 3.95 FAMGGALQGYY134 pKa = 9.55 LIDD137 pKa = 3.99 KK138 pKa = 8.32 ATAVEE143 pKa = 4.09 YY144 pKa = 10.49 GITSIEE150 pKa = 4.18 DD151 pKa = 3.46 FKK153 pKa = 11.56 DD154 pKa = 3.36 PEE156 pKa = 3.8 IAALFDD162 pKa = 3.62 TDD164 pKa = 4.6 GDD166 pKa = 3.96 GKK168 pKa = 10.96 ANMVACPPGWGCEE181 pKa = 4.01 TVINHH186 pKa = 6.0 HH187 pKa = 5.89 MEE189 pKa = 4.38 AYY191 pKa = 9.83 EE192 pKa = 4.06 LADD195 pKa = 3.46 WVNADD200 pKa = 3.28 QAGYY204 pKa = 9.82 DD205 pKa = 3.36 VAMADD210 pKa = 3.33 VVARR214 pKa = 11.84 YY215 pKa = 8.59 NAGEE219 pKa = 3.95 PVFFYY224 pKa = 9.96 TWTPNWTVNALVPGEE239 pKa = 4.07 DD240 pKa = 4.01 VVWLEE245 pKa = 4.49 VPFSSLPADD254 pKa = 3.58 QADD257 pKa = 4.21 LEE259 pKa = 4.91 DD260 pKa = 3.72 EE261 pKa = 4.48 TFVANLVGKK270 pKa = 10.3 AGDD273 pKa = 3.82 SEE275 pKa = 4.83 PYY277 pKa = 10.48 NMGWPANDD285 pKa = 3.04 IQVVANSAFLDD296 pKa = 3.94 ANPAAAYY303 pKa = 10.12 LFEE306 pKa = 5.58 NIQIPLEE313 pKa = 4.81 DD314 pKa = 3.65 IFEE317 pKa = 4.43 QNYY320 pKa = 9.63 QMYY323 pKa = 9.96 QGEE326 pKa = 4.85 DD327 pKa = 3.24 RR328 pKa = 11.84 PEE330 pKa = 5.9 DD331 pKa = 3.38 IVAHH335 pKa = 5.81 AEE337 pKa = 3.41 AWIAANQSTFDD348 pKa = 3.25 AWIEE352 pKa = 4.05 GAIDD356 pKa = 3.55 AADD359 pKa = 3.07
Molecular weight: 39.39 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.695
IPC2_protein 3.77
IPC_protein 3.783
Toseland 3.567
ProMoST 3.91
Dawson 3.77
Bjellqvist 3.935
Wikipedia 3.681
Rodwell 3.605
Grimsley 3.478
Solomon 3.757
Lehninger 3.719
Nozaki 3.872
DTASelect 4.088
Thurlkill 3.617
EMBOSS 3.694
Sillero 3.897
Patrickios 1.265
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.814
Protein with the highest isoelectric point:
>tr|D8K001|D8K001_DEHLB Lysine--tRNA ligase OS=Dehalogenimonas lykanthroporepellens (strain ATCC BAA-1523 / JCM 15061 / BL-DC-9) OX=552811 GN=lysS PE=3 SV=1
MM1 pKa = 7.42 NLTLEE6 pKa = 4.24 YY7 pKa = 10.32 LLSVFIAALAVIQLGAVRR25 pKa = 11.84 AGLRR29 pKa = 11.84 GLYY32 pKa = 10.21 LGGSRR37 pKa = 11.84 LVACLVAVPCLLISGILFFTWNDD60 pKa = 3.39 RR61 pKa = 11.84 NPTGVIEE68 pKa = 4.6 GAQQAGLFSLAVAAAAAFTVILGLITNRR96 pKa = 11.84 RR97 pKa = 11.84 MPADD101 pKa = 3.93 RR102 pKa = 11.84 PTSTEE107 pKa = 3.58 GLEE110 pKa = 3.96 ALKK113 pKa = 9.73 RR114 pKa = 11.84 TTFIHH119 pKa = 7.54 AIRR122 pKa = 11.84 SRR124 pKa = 11.84 FWKK127 pKa = 10.5 SS128 pKa = 2.65
Molecular weight: 13.75 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.344
IPC2_protein 9.531
IPC_protein 10.526
Toseland 10.452
ProMoST 10.277
Dawson 10.599
Bjellqvist 10.394
Wikipedia 10.862
Rodwell 10.628
Grimsley 10.672
Solomon 10.745
Lehninger 10.701
Nozaki 10.496
DTASelect 10.365
Thurlkill 10.482
EMBOSS 10.862
Sillero 10.54
Patrickios 10.452
IPC_peptide 10.745
IPC2_peptide 9.736
IPC2.peptide.svr19 8.455
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1619
0
1619
477317
31
1597
294.8
32.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.652 ± 0.083
1.103 ± 0.027
5.545 ± 0.046
6.754 ± 0.063
3.695 ± 0.043
8.105 ± 0.065
1.911 ± 0.024
6.405 ± 0.057
4.419 ± 0.057
9.948 ± 0.063
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.407 ± 0.031
3.328 ± 0.039
4.611 ± 0.034
3.145 ± 0.033
6.528 ± 0.066
5.684 ± 0.043
5.559 ± 0.049
7.339 ± 0.054
1.102 ± 0.026
2.76 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here