Salmonella phage LSPA1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Guernseyvirinae; Jerseyvirus; Salmonella virus LSPA1

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 58 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A088F847|A0A088F847_9CAUD Uncharacterized protein OS=Salmonella phage LSPA1 OX=1540823 GN=LSPA1_47 PE=4 SV=1
MM1 pKa = 7.13NWVSVNEE8 pKa = 4.29ALPEE12 pKa = 4.32SKK14 pKa = 10.37DD15 pKa = 3.7DD16 pKa = 3.86SVLVCSVDD24 pKa = 3.1GSKK27 pKa = 11.13YY28 pKa = 10.46DD29 pKa = 4.26DD30 pKa = 3.46NGFPEE35 pKa = 4.53GGIDD39 pKa = 3.59FVHH42 pKa = 6.27IQDD45 pKa = 4.0YY46 pKa = 10.83FDD48 pKa = 6.61DD49 pKa = 3.76ITAGLDD55 pKa = 3.29EE56 pKa = 5.5NGNQLYY62 pKa = 7.47TKK64 pKa = 9.84RR65 pKa = 11.84YY66 pKa = 7.78IEE68 pKa = 3.98MGITHH73 pKa = 7.23WMYY76 pKa = 11.09LPEE79 pKa = 5.3LPEE82 pKa = 4.17DD83 pKa = 3.73AKK85 pKa = 11.43

Molecular weight: 9.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A088FC34|A0A088FC34_9CAUD Uncharacterized protein OS=Salmonella phage LSPA1 OX=1540823 GN=LSPA1_56 PE=4 SV=1
MM1 pKa = 7.52SLATDD6 pKa = 3.44ILKK9 pKa = 10.53RR10 pKa = 11.84AGLDD14 pKa = 3.35LAPPSSAVTKK24 pKa = 10.32KK25 pKa = 7.19VTRR28 pKa = 11.84VKK30 pKa = 10.48EE31 pKa = 3.74KK32 pKa = 10.81AKK34 pKa = 10.52RR35 pKa = 11.84KK36 pKa = 8.28PKK38 pKa = 9.97PQAKK42 pKa = 9.12PLNEE46 pKa = 4.14MPDD49 pKa = 3.63VYY51 pKa = 10.82PRR53 pKa = 11.84IPGVHH58 pKa = 4.79QPKK61 pKa = 9.28YY62 pKa = 10.21CAGKK66 pKa = 10.09GLWRR70 pKa = 11.84AHH72 pKa = 6.43SYY74 pKa = 10.54DD75 pKa = 3.01GKK77 pKa = 10.98KK78 pKa = 10.05VVNLGEE84 pKa = 4.35FSSQARR90 pKa = 11.84AHH92 pKa = 6.01MAVKK96 pKa = 10.46LYY98 pKa = 10.67KK99 pKa = 9.81LWRR102 pKa = 11.84KK103 pKa = 9.46RR104 pKa = 11.84GYY106 pKa = 10.35SEE108 pKa = 5.16IPHH111 pKa = 6.33KK112 pKa = 10.54PSIRR116 pKa = 11.84LYY118 pKa = 8.83TFRR121 pKa = 6.0

Molecular weight: 13.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

58

0

58

12561

41

852

216.6

23.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.569 ± 0.66

0.947 ± 0.129

5.979 ± 0.203

6.799 ± 0.405

3.758 ± 0.161

7.905 ± 0.302

1.672 ± 0.177

4.872 ± 0.263

5.979 ± 0.389

7.826 ± 0.343

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.699 ± 0.169

4.538 ± 0.27

3.893 ± 0.197

3.614 ± 0.209

5.692 ± 0.247

5.7 ± 0.297

6.504 ± 0.345

7.364 ± 0.314

1.393 ± 0.165

3.296 ± 0.207
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski