Amino acid dipepetide frequency for Salmonella phage LSPA1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.863AlaAla: 11.863 ± 1.761
0.796AlaCys: 0.796 ± 0.25
5.892AlaAsp: 5.892 ± 0.778
6.449AlaGlu: 6.449 ± 0.915
3.583AlaPhe: 3.583 ± 0.475
7.723AlaGly: 7.723 ± 0.764
1.752AlaHis: 1.752 ± 0.309
4.14AlaIle: 4.14 ± 0.663
5.573AlaLys: 5.573 ± 0.854
7.803AlaLeu: 7.803 ± 0.808
2.389AlaMet: 2.389 ± 0.552
3.424AlaAsn: 3.424 ± 0.566
2.787AlaPro: 2.787 ± 0.555
3.424AlaGln: 3.424 ± 0.726
4.777AlaArg: 4.777 ± 0.454
6.051AlaSer: 6.051 ± 0.799
5.573AlaThr: 5.573 ± 0.598
6.768AlaVal: 6.768 ± 0.809
1.433AlaTrp: 1.433 ± 0.358
3.424AlaTyr: 3.424 ± 0.516
0.0AlaXaa: 0.0 ± 0.0
Cys
0.876CysAla: 0.876 ± 0.239
0.159CysCys: 0.159 ± 0.105
0.717CysAsp: 0.717 ± 0.254
0.955CysGlu: 0.955 ± 0.256
0.159CysPhe: 0.159 ± 0.11
0.637CysGly: 0.637 ± 0.217
0.08CysHis: 0.08 ± 0.086
0.398CysIle: 0.398 ± 0.224
0.478CysLys: 0.478 ± 0.213
0.557CysLeu: 0.557 ± 0.334
0.239CysMet: 0.239 ± 0.118
0.478CysAsn: 0.478 ± 0.259
0.239CysPro: 0.239 ± 0.176
0.398CysGln: 0.398 ± 0.17
0.478CysArg: 0.478 ± 0.216
0.637CysSer: 0.637 ± 0.196
0.557CysThr: 0.557 ± 0.206
0.637CysVal: 0.637 ± 0.233
0.239CysTrp: 0.239 ± 0.13
0.557CysTyr: 0.557 ± 0.191
0.0CysXaa: 0.0 ± 0.0
Asp
5.732AspAla: 5.732 ± 0.831
0.637AspCys: 0.637 ± 0.29
4.299AspAsp: 4.299 ± 0.611
3.981AspGlu: 3.981 ± 0.547
3.742AspPhe: 3.742 ± 0.488
6.29AspGly: 6.29 ± 0.82
0.717AspHis: 0.717 ± 0.249
3.742AspIle: 3.742 ± 0.445
2.787AspLys: 2.787 ± 0.607
5.255AspLeu: 5.255 ± 0.597
1.354AspMet: 1.354 ± 0.255
2.707AspAsn: 2.707 ± 0.443
1.752AspPro: 1.752 ± 0.427
0.478AspGln: 0.478 ± 0.166
2.627AspArg: 2.627 ± 0.395
2.946AspSer: 2.946 ± 0.403
3.981AspThr: 3.981 ± 0.482
4.14AspVal: 4.14 ± 0.38
0.876AspTrp: 0.876 ± 0.332
1.752AspTyr: 1.752 ± 0.295
0.0AspXaa: 0.0 ± 0.0
Glu
6.768GluAla: 6.768 ± 0.693
0.318GluCys: 0.318 ± 0.159
4.061GluAsp: 4.061 ± 0.381
5.016GluGlu: 5.016 ± 0.903
3.344GluPhe: 3.344 ± 0.699
4.459GluGly: 4.459 ± 0.718
0.717GluHis: 0.717 ± 0.213
3.901GluIle: 3.901 ± 0.567
4.538GluLys: 4.538 ± 0.693
5.494GluLeu: 5.494 ± 0.669
2.787GluMet: 2.787 ± 0.419
2.866GluAsn: 2.866 ± 0.432
1.831GluPro: 1.831 ± 0.525
3.344GluGln: 3.344 ± 0.743
4.14GluArg: 4.14 ± 0.63
3.822GluSer: 3.822 ± 0.557
3.742GluThr: 3.742 ± 0.67
4.459GluVal: 4.459 ± 0.405
0.876GluTrp: 0.876 ± 0.346
1.513GluTyr: 1.513 ± 0.374
0.0GluXaa: 0.0 ± 0.0
Phe
2.707PheAla: 2.707 ± 0.471
0.478PheCys: 0.478 ± 0.175
3.344PheAsp: 3.344 ± 0.443
2.946PheGlu: 2.946 ± 0.583
1.035PhePhe: 1.035 ± 0.245
3.344PheGly: 3.344 ± 0.464
0.717PheHis: 0.717 ± 0.214
2.627PheIle: 2.627 ± 0.459
1.354PheLys: 1.354 ± 0.283
1.831PheLeu: 1.831 ± 0.318
0.398PheMet: 0.398 ± 0.149
1.433PheAsn: 1.433 ± 0.389
1.592PhePro: 1.592 ± 0.39
1.513PheGln: 1.513 ± 0.344
2.15PheArg: 2.15 ± 0.293
2.787PheSer: 2.787 ± 0.418
3.105PheThr: 3.105 ± 0.625
2.229PheVal: 2.229 ± 0.421
0.876PheTrp: 0.876 ± 0.326
1.115PheTyr: 1.115 ± 0.301
0.0PheXaa: 0.0 ± 0.0
Gly
6.608GlyAla: 6.608 ± 0.68
0.955GlyCys: 0.955 ± 0.238
4.459GlyAsp: 4.459 ± 0.531
5.892GlyGlu: 5.892 ± 0.686
2.787GlyPhe: 2.787 ± 0.437
6.847GlyGly: 6.847 ± 1.144
1.274GlyHis: 1.274 ± 0.477
3.264GlyIle: 3.264 ± 0.579
5.653GlyLys: 5.653 ± 0.744
5.812GlyLeu: 5.812 ± 0.643
2.229GlyMet: 2.229 ± 0.685
4.379GlyAsn: 4.379 ± 0.503
1.592GlyPro: 1.592 ± 0.372
2.787GlyGln: 2.787 ± 0.42
4.459GlyArg: 4.459 ± 0.551
5.334GlySer: 5.334 ± 0.57
4.379GlyThr: 4.379 ± 0.537
6.529GlyVal: 6.529 ± 0.852
1.115GlyTrp: 1.115 ± 0.295
2.707GlyTyr: 2.707 ± 0.537
0.0GlyXaa: 0.0 ± 0.0
His
1.194HisAla: 1.194 ± 0.254
0.318HisCys: 0.318 ± 0.178
1.035HisAsp: 1.035 ± 0.292
0.717HisGlu: 0.717 ± 0.265
0.398HisPhe: 0.398 ± 0.178
1.035HisGly: 1.035 ± 0.338
1.115HisHis: 1.115 ± 0.265
0.955HisIle: 0.955 ± 0.253
1.274HisLys: 1.274 ± 0.316
1.115HisLeu: 1.115 ± 0.382
0.557HisMet: 0.557 ± 0.22
0.717HisAsn: 0.717 ± 0.258
0.876HisPro: 0.876 ± 0.305
1.035HisGln: 1.035 ± 0.286
0.717HisArg: 0.717 ± 0.238
1.035HisSer: 1.035 ± 0.301
0.796HisThr: 0.796 ± 0.334
1.354HisVal: 1.354 ± 0.408
0.08HisTrp: 0.08 ± 0.071
0.398HisTyr: 0.398 ± 0.223
0.0HisXaa: 0.0 ± 0.0
Ile
4.777IleAla: 4.777 ± 0.772
0.557IleCys: 0.557 ± 0.178
3.424IleAsp: 3.424 ± 0.339
2.627IleGlu: 2.627 ± 0.421
1.433IlePhe: 1.433 ± 0.347
3.185IleGly: 3.185 ± 0.517
0.398IleHis: 0.398 ± 0.155
2.627IleIle: 2.627 ± 0.509
3.025IleLys: 3.025 ± 0.546
2.707IleLeu: 2.707 ± 0.452
1.194IleMet: 1.194 ± 0.282
3.105IleAsn: 3.105 ± 0.507
2.627IlePro: 2.627 ± 0.527
1.831IleGln: 1.831 ± 0.368
2.548IleArg: 2.548 ± 0.371
2.627IleSer: 2.627 ± 0.514
4.936IleThr: 4.936 ± 0.655
2.946IleVal: 2.946 ± 0.469
1.115IleTrp: 1.115 ± 0.315
1.035IleTyr: 1.035 ± 0.403
0.0IleXaa: 0.0 ± 0.0
Lys
5.414LysAla: 5.414 ± 0.961
0.796LysCys: 0.796 ± 0.284
3.583LysAsp: 3.583 ± 0.539
4.299LysGlu: 4.299 ± 0.588
1.911LysPhe: 1.911 ± 0.296
3.742LysGly: 3.742 ± 0.465
1.433LysHis: 1.433 ± 0.291
1.831LysIle: 1.831 ± 0.419
3.424LysLys: 3.424 ± 0.547
5.573LysLeu: 5.573 ± 0.625
3.025LysMet: 3.025 ± 0.6
2.389LysAsn: 2.389 ± 0.438
3.025LysPro: 3.025 ± 0.723
2.15LysGln: 2.15 ± 0.455
4.618LysArg: 4.618 ± 0.789
2.229LysSer: 2.229 ± 0.622
3.901LysThr: 3.901 ± 0.504
3.264LysVal: 3.264 ± 0.67
0.478LysTrp: 0.478 ± 0.187
2.707LysTyr: 2.707 ± 0.398
0.0LysXaa: 0.0 ± 0.0
Leu
7.166LeuAla: 7.166 ± 0.706
1.115LeuCys: 1.115 ± 0.411
4.22LeuAsp: 4.22 ± 0.613
4.936LeuGlu: 4.936 ± 0.678
2.15LeuPhe: 2.15 ± 0.383
5.255LeuGly: 5.255 ± 0.5
0.796LeuHis: 0.796 ± 0.274
4.618LeuIle: 4.618 ± 0.508
5.334LeuLys: 5.334 ± 0.798
7.166LeuLeu: 7.166 ± 0.904
1.752LeuMet: 1.752 ± 0.313
4.459LeuAsn: 4.459 ± 0.726
4.14LeuPro: 4.14 ± 0.616
2.07LeuGln: 2.07 ± 0.418
5.175LeuArg: 5.175 ± 0.653
4.299LeuSer: 4.299 ± 0.809
5.175LeuThr: 5.175 ± 0.526
5.096LeuVal: 5.096 ± 0.556
0.876LeuTrp: 0.876 ± 0.367
2.468LeuTyr: 2.468 ± 0.509
0.0LeuXaa: 0.0 ± 0.0
Met
2.627MetAla: 2.627 ± 0.39
0.08MetCys: 0.08 ± 0.077
1.115MetAsp: 1.115 ± 0.34
1.354MetGlu: 1.354 ± 0.328
0.955MetPhe: 0.955 ± 0.284
1.99MetGly: 1.99 ± 0.32
0.637MetHis: 0.637 ± 0.222
1.354MetIle: 1.354 ± 0.362
1.592MetLys: 1.592 ± 0.334
2.15MetLeu: 2.15 ± 0.49
0.717MetMet: 0.717 ± 0.267
0.717MetAsn: 0.717 ± 0.286
1.274MetPro: 1.274 ± 0.388
0.717MetGln: 0.717 ± 0.226
1.672MetArg: 1.672 ± 0.313
1.752MetSer: 1.752 ± 0.298
2.627MetThr: 2.627 ± 0.411
2.309MetVal: 2.309 ± 0.456
0.478MetTrp: 0.478 ± 0.178
0.876MetTyr: 0.876 ± 0.269
0.0MetXaa: 0.0 ± 0.0
Asn
4.379AsnAla: 4.379 ± 0.532
0.478AsnCys: 0.478 ± 0.221
2.15AsnAsp: 2.15 ± 0.418
3.583AsnGlu: 3.583 ± 0.441
1.354AsnPhe: 1.354 ± 0.274
3.503AsnGly: 3.503 ± 0.605
0.398AsnHis: 0.398 ± 0.168
2.866AsnIle: 2.866 ± 0.499
2.468AsnLys: 2.468 ± 0.382
3.503AsnLeu: 3.503 ± 0.439
0.955AsnMet: 0.955 ± 0.494
2.07AsnAsn: 2.07 ± 0.435
2.15AsnPro: 2.15 ± 0.503
1.513AsnGln: 1.513 ± 0.439
2.309AsnArg: 2.309 ± 0.315
2.309AsnSer: 2.309 ± 0.357
2.15AsnThr: 2.15 ± 0.416
4.777AsnVal: 4.777 ± 0.53
0.955AsnTrp: 0.955 ± 0.282
1.513AsnTyr: 1.513 ± 0.372
0.0AsnXaa: 0.0 ± 0.0
Pro
2.787ProAla: 2.787 ± 0.4
0.318ProCys: 0.318 ± 0.155
2.707ProAsp: 2.707 ± 0.511
4.618ProGlu: 4.618 ± 0.669
1.672ProPhe: 1.672 ± 0.282
2.627ProGly: 2.627 ± 0.526
0.876ProHis: 0.876 ± 0.235
1.035ProIle: 1.035 ± 0.337
2.15ProLys: 2.15 ± 0.44
3.344ProLeu: 3.344 ± 0.484
0.876ProMet: 0.876 ± 0.315
1.911ProAsn: 1.911 ± 0.534
1.274ProPro: 1.274 ± 0.329
1.274ProGln: 1.274 ± 0.288
2.07ProArg: 2.07 ± 0.407
2.309ProSer: 2.309 ± 0.429
1.752ProThr: 1.752 ± 0.351
3.662ProVal: 3.662 ± 0.539
0.398ProTrp: 0.398 ± 0.167
1.274ProTyr: 1.274 ± 0.339
0.0ProXaa: 0.0 ± 0.0
Gln
3.901GlnAla: 3.901 ± 0.612
0.159GlnCys: 0.159 ± 0.104
1.831GlnAsp: 1.831 ± 0.296
2.468GlnGlu: 2.468 ± 0.525
0.955GlnPhe: 0.955 ± 0.344
2.468GlnGly: 2.468 ± 0.396
0.557GlnHis: 0.557 ± 0.242
1.911GlnIle: 1.911 ± 0.38
2.15GlnLys: 2.15 ± 0.492
3.264GlnLeu: 3.264 ± 0.486
1.115GlnMet: 1.115 ± 0.295
1.672GlnAsn: 1.672 ± 0.27
1.911GlnPro: 1.911 ± 0.338
1.752GlnGln: 1.752 ± 0.541
1.99GlnArg: 1.99 ± 0.402
1.194GlnSer: 1.194 ± 0.319
2.15GlnThr: 2.15 ± 0.389
2.787GlnVal: 2.787 ± 0.435
0.478GlnTrp: 0.478 ± 0.183
1.433GlnTyr: 1.433 ± 0.32
0.0GlnXaa: 0.0 ± 0.0
Arg
5.016ArgAla: 5.016 ± 0.623
0.239ArgCys: 0.239 ± 0.151
2.946ArgAsp: 2.946 ± 0.448
2.946ArgGlu: 2.946 ± 0.482
2.07ArgPhe: 2.07 ± 0.391
4.936ArgGly: 4.936 ± 0.481
1.354ArgHis: 1.354 ± 0.376
2.548ArgIle: 2.548 ± 0.372
3.901ArgLys: 3.901 ± 0.701
4.299ArgLeu: 4.299 ± 0.527
1.831ArgMet: 1.831 ± 0.326
3.185ArgAsn: 3.185 ± 0.511
1.672ArgPro: 1.672 ± 0.343
3.503ArgGln: 3.503 ± 0.457
4.299ArgArg: 4.299 ± 0.558
2.229ArgSer: 2.229 ± 0.308
2.866ArgThr: 2.866 ± 0.41
4.857ArgVal: 4.857 ± 0.59
0.796ArgTrp: 0.796 ± 0.211
1.433ArgTyr: 1.433 ± 0.414
0.0ArgXaa: 0.0 ± 0.0
Ser
5.653SerAla: 5.653 ± 0.972
0.318SerCys: 0.318 ± 0.135
3.105SerAsp: 3.105 ± 0.525
3.185SerGlu: 3.185 ± 0.441
2.627SerPhe: 2.627 ± 0.437
5.892SerGly: 5.892 ± 0.691
0.796SerHis: 0.796 ± 0.301
2.548SerIle: 2.548 ± 0.435
2.787SerLys: 2.787 ± 0.473
4.857SerLeu: 4.857 ± 0.537
1.274SerMet: 1.274 ± 0.313
2.866SerAsn: 2.866 ± 0.444
1.99SerPro: 1.99 ± 0.466
1.911SerGln: 1.911 ± 0.363
3.185SerArg: 3.185 ± 0.459
2.389SerSer: 2.389 ± 0.508
4.14SerThr: 4.14 ± 0.608
4.697SerVal: 4.697 ± 0.698
0.955SerTrp: 0.955 ± 0.271
1.831SerTyr: 1.831 ± 0.377
0.0SerXaa: 0.0 ± 0.0
Thr
6.927ThrAla: 6.927 ± 0.763
0.318ThrCys: 0.318 ± 0.137
3.742ThrAsp: 3.742 ± 0.578
2.946ThrGlu: 2.946 ± 0.404
2.946ThrPhe: 2.946 ± 0.56
6.369ThrGly: 6.369 ± 0.867
1.035ThrHis: 1.035 ± 0.284
3.185ThrIle: 3.185 ± 0.438
3.424ThrLys: 3.424 ± 0.562
4.459ThrLeu: 4.459 ± 0.724
1.274ThrMet: 1.274 ± 0.41
1.99ThrAsn: 1.99 ± 0.406
4.22ThrPro: 4.22 ± 0.476
1.831ThrGln: 1.831 ± 0.392
3.025ThrArg: 3.025 ± 0.394
4.777ThrSer: 4.777 ± 0.726
4.459ThrThr: 4.459 ± 0.562
4.538ThrVal: 4.538 ± 0.691
0.955ThrTrp: 0.955 ± 0.235
2.627ThrTyr: 2.627 ± 0.473
0.0ThrXaa: 0.0 ± 0.0
Val
7.086ValAla: 7.086 ± 0.789
0.876ValCys: 0.876 ± 0.29
4.538ValAsp: 4.538 ± 0.461
5.653ValGlu: 5.653 ± 0.497
2.468ValPhe: 2.468 ± 0.413
4.697ValGly: 4.697 ± 0.56
0.876ValHis: 0.876 ± 0.209
3.742ValIle: 3.742 ± 0.559
4.697ValLys: 4.697 ± 0.492
5.653ValLeu: 5.653 ± 0.832
1.513ValMet: 1.513 ± 0.361
3.105ValAsn: 3.105 ± 0.464
2.468ValPro: 2.468 ± 0.557
2.389ValGln: 2.389 ± 0.408
3.583ValArg: 3.583 ± 0.482
5.732ValSer: 5.732 ± 0.697
5.653ValThr: 5.653 ± 0.682
5.414ValVal: 5.414 ± 0.623
0.637ValTrp: 0.637 ± 0.286
2.866ValTyr: 2.866 ± 0.378
0.0ValXaa: 0.0 ± 0.0
Trp
1.513TrpAla: 1.513 ± 0.567
0.159TrpCys: 0.159 ± 0.109
0.876TrpAsp: 0.876 ± 0.286
0.717TrpGlu: 0.717 ± 0.209
0.796TrpPhe: 0.796 ± 0.285
0.955TrpGly: 0.955 ± 0.254
0.478TrpHis: 0.478 ± 0.229
0.318TrpIle: 0.318 ± 0.149
0.318TrpLys: 0.318 ± 0.165
1.354TrpLeu: 1.354 ± 0.356
0.557TrpMet: 0.557 ± 0.197
0.796TrpAsn: 0.796 ± 0.286
0.398TrpPro: 0.398 ± 0.216
0.557TrpGln: 0.557 ± 0.18
0.876TrpArg: 0.876 ± 0.307
0.796TrpSer: 0.796 ± 0.25
0.717TrpThr: 0.717 ± 0.242
1.354TrpVal: 1.354 ± 0.325
0.159TrpTrp: 0.159 ± 0.109
0.239TrpTyr: 0.239 ± 0.163
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.707TyrAla: 2.707 ± 0.467
0.398TyrCys: 0.398 ± 0.193
1.752TyrAsp: 1.752 ± 0.469
2.707TyrGlu: 2.707 ± 0.339
1.194TyrPhe: 1.194 ± 0.332
3.105TyrGly: 3.105 ± 0.422
0.717TyrHis: 0.717 ± 0.208
1.115TyrIle: 1.115 ± 0.295
2.866TyrLys: 2.866 ± 0.412
2.07TyrLeu: 2.07 ± 0.345
0.876TyrMet: 0.876 ± 0.199
1.115TyrAsn: 1.115 ± 0.272
1.115TyrPro: 1.115 ± 0.331
1.672TyrGln: 1.672 ± 0.429
2.229TyrArg: 2.229 ± 0.428
1.752TyrSer: 1.752 ± 0.351
2.389TyrThr: 2.389 ± 0.44
1.831TyrVal: 1.831 ± 0.294
0.159TyrTrp: 0.159 ± 0.119
1.194TyrTyr: 1.194 ± 0.237
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 58 proteins (12561 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski