Alfalfa latent virus
Average proteome isoelectric point is 6.33
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0C5H7M3|A0A0C5H7M3_9VIRU Helicase OS=Alfalfa latent virus OX=165250 PE=3 SV=1
MM1 pKa = 7.47 QLFQVILSVFSCCAVLLCLYY21 pKa = 10.07 TIDD24 pKa = 4.58 SFLNNSVCQCTVVLTGEE41 pKa = 4.12 SVKK44 pKa = 10.54 IVGCEE49 pKa = 3.77 FTSEE53 pKa = 4.24 FIEE56 pKa = 4.26 YY57 pKa = 10.66 AKK59 pKa = 9.63 TLKK62 pKa = 10.35 VQAII66 pKa = 4.02
Molecular weight: 7.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.763
IPC2_protein 4.902
IPC_protein 4.507
Toseland 4.368
ProMoST 4.596
Dawson 4.418
Bjellqvist 4.584
Wikipedia 4.279
Rodwell 4.355
Grimsley 4.291
Solomon 4.406
Lehninger 4.355
Nozaki 4.558
DTASelect 4.584
Thurlkill 4.393
EMBOSS 4.304
Sillero 4.609
Patrickios 0.006
IPC_peptide 4.418
IPC2_peptide 4.596
IPC2.peptide.svr19 4.591
Protein with the highest isoelectric point:
>tr|Q913Z7|Q913Z7_9VIRU Movement protein TGB2 OS=Alfalfa latent virus OX=165250 PE=3 SV=1
MM1 pKa = 7.61 PLIAPPNNSNSYY13 pKa = 9.8 LALAIGAGFAIIIFTLRR30 pKa = 11.84 SNQLPHH36 pKa = 7.87 VGDD39 pKa = 4.79 NIHH42 pKa = 6.42 SLPHH46 pKa = 5.56 GGFYY50 pKa = 10.28 RR51 pKa = 11.84 DD52 pKa = 3.42 GTKK55 pKa = 10.29 VIQYY59 pKa = 8.2 NSPVRR64 pKa = 11.84 TPNNWFKK71 pKa = 11.32 GPNNIQALALVLLVIGLIHH90 pKa = 6.83 ASSVKK95 pKa = 9.98 ISRR98 pKa = 11.84 GCSCSKK104 pKa = 10.9
Molecular weight: 11.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.239
IPC2_protein 9.443
IPC_protein 9.589
Toseland 9.955
ProMoST 9.853
Dawson 10.189
Bjellqvist 9.926
Wikipedia 10.379
Rodwell 10.54
Grimsley 10.277
Solomon 10.248
Lehninger 10.218
Nozaki 10.043
DTASelect 9.897
Thurlkill 10.028
EMBOSS 10.365
Sillero 10.131
Patrickios 10.292
IPC_peptide 10.248
IPC2_peptide 8.96
IPC2.peptide.svr19 8.283
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
2616
66
1908
523.2
59.26
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.766 ± 1.24
2.638 ± 0.389
5.505 ± 0.436
7.989 ± 0.968
5.81 ± 0.31
6.078 ± 0.266
2.332 ± 0.518
5.925 ± 0.637
6.46 ± 0.451
10.512 ± 1.29
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.911 ± 0.276
4.472 ± 0.503
3.517 ± 0.664
3.058 ± 0.733
5.505 ± 0.395
7.148 ± 0.489
4.205 ± 0.413
6.078 ± 0.781
0.994 ± 0.241
3.096 ± 0.647
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here