Aspergillus saccharolyticus JOP 1030-1

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus; Aspergillus saccharolyticus

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10061 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A319ACF1|A0A319ACF1_9EURO Uncharacterized protein OS=Aspergillus saccharolyticus JOP 1030-1 OX=1450539 GN=BP01DRAFT_388382 PE=4 SV=1
MM1 pKa = 7.11VRR3 pKa = 11.84QLALACGLLAAVAVQAAPAEE23 pKa = 4.41PAHH26 pKa = 6.16PMVTEE31 pKa = 4.51APDD34 pKa = 3.27ASLLHH39 pKa = 6.49KK40 pKa = 10.48RR41 pKa = 11.84ATTCTFSGSEE51 pKa = 4.19GASKK55 pKa = 10.64VSKK58 pKa = 10.85SKK60 pKa = 9.78TDD62 pKa = 2.91CSTIYY67 pKa = 10.59LSALAVPSGTTLDD80 pKa = 4.91LKK82 pKa = 10.98DD83 pKa = 5.0LNDD86 pKa = 3.71GTHH89 pKa = 6.5VIFEE93 pKa = 4.49GEE95 pKa = 4.12TTFGYY100 pKa = 10.01EE101 pKa = 3.67EE102 pKa = 4.39WEE104 pKa = 4.59GPLVSVSGTDD114 pKa = 3.1ITVEE118 pKa = 4.17GASGAVLNGDD128 pKa = 4.21GSRR131 pKa = 11.84WWDD134 pKa = 3.48GEE136 pKa = 3.94GSDD139 pKa = 5.38GGKK142 pKa = 8.39TKK144 pKa = 10.66PKK146 pKa = 10.23FFAAHH151 pKa = 7.33DD152 pKa = 4.23LTSSTIKK159 pKa = 10.41SIYY162 pKa = 9.57IEE164 pKa = 4.05NSPVQVFSIDD174 pKa = 3.15GSTDD178 pKa = 2.92LTMTDD183 pKa = 2.82ITIDD187 pKa = 3.65NTDD190 pKa = 3.45GDD192 pKa = 4.42TDD194 pKa = 4.09DD195 pKa = 5.53LAANTDD201 pKa = 3.28GFDD204 pKa = 3.25IGEE207 pKa = 4.38STDD210 pKa = 3.19ITITGATVYY219 pKa = 10.89NQDD222 pKa = 3.35DD223 pKa = 3.99CVAINSGEE231 pKa = 4.14NIYY234 pKa = 10.35FAAGVCSGGHH244 pKa = 5.63GLSIGSVGGRR254 pKa = 11.84DD255 pKa = 3.68DD256 pKa = 3.79NTVKK260 pKa = 10.76NVTFYY265 pKa = 11.32DD266 pKa = 3.84VNVLKK271 pKa = 10.54SQQAIRR277 pKa = 11.84IKK279 pKa = 10.53AIYY282 pKa = 9.49GDD284 pKa = 3.76TGSISDD290 pKa = 3.48ITYY293 pKa = 10.53HH294 pKa = 6.92EE295 pKa = 4.44IAFSDD300 pKa = 3.41ATDD303 pKa = 3.42YY304 pKa = 11.77GIVVEE309 pKa = 4.53QNYY312 pKa = 10.88DD313 pKa = 3.64DD314 pKa = 3.92TSKK317 pKa = 9.89TPTTGIPITDD327 pKa = 4.11FTLEE331 pKa = 4.32TIIGTCADD339 pKa = 4.59DD340 pKa = 4.66DD341 pKa = 4.05CTDD344 pKa = 3.21IYY346 pKa = 10.48IACGSGACSDD356 pKa = 3.8WSWSGVSVTGGKK368 pKa = 10.1VSTDD372 pKa = 3.51CLNVPSGISCDD383 pKa = 3.58LL384 pKa = 3.82

Molecular weight: 40.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A318Z116|A0A318Z116_9EURO Serine/threonine-protein kinase bur1 OS=Aspergillus saccharolyticus JOP 1030-1 OX=1450539 GN=BP01DRAFT_361075 PE=4 SV=1
MM1 pKa = 7.88PSHH4 pKa = 6.91KK5 pKa = 10.39SFRR8 pKa = 11.84TKK10 pKa = 10.45QKK12 pKa = 9.54LAKK15 pKa = 9.74AQRR18 pKa = 11.84QNRR21 pKa = 11.84PIPQWIRR28 pKa = 11.84LRR30 pKa = 11.84TGNTIRR36 pKa = 11.84YY37 pKa = 5.79NAKK40 pKa = 8.89RR41 pKa = 11.84RR42 pKa = 11.84HH43 pKa = 4.14WRR45 pKa = 11.84KK46 pKa = 7.41TRR48 pKa = 11.84LGII51 pKa = 4.46

Molecular weight: 6.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10061

0

10061

4793634

49

6402

476.5

52.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.857 ± 0.023

1.277 ± 0.011

5.476 ± 0.019

6.115 ± 0.025

3.66 ± 0.017

6.725 ± 0.021

2.529 ± 0.011

4.764 ± 0.018

4.287 ± 0.022

9.236 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.105 ± 0.009

3.432 ± 0.011

6.101 ± 0.024

4.131 ± 0.015

6.325 ± 0.022

8.357 ± 0.031

6.124 ± 0.018

6.21 ± 0.019

1.461 ± 0.01

2.827 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski