Channa argus (northern snakehead)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Actinopterygii; Actinopteri; Neopterygii; Teleostei; Osteoglossocephalai; Clupeocephala; Euteleoste

Average proteome isoelectric point is 6.62

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 22260 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G1Q080|A0A6G1Q080_9TELE Centrosomal protein POC5 OS=Channa argus OX=215402 GN=EXN66_Car011555 PE=3 SV=1
MM1 pKa = 7.33EE2 pKa = 5.33RR3 pKa = 11.84VDD5 pKa = 5.41NLLEE9 pKa = 4.44DD10 pKa = 3.53WTEE13 pKa = 3.88SLDD16 pKa = 3.43IFGSVWIDD24 pKa = 3.2VLGVPRR30 pKa = 11.84APQDD34 pKa = 3.03WSICLNLLEE43 pKa = 4.91LLCLDD48 pKa = 4.13VLSQVDD54 pKa = 3.9FLLEE58 pKa = 3.48HH59 pKa = 6.86RR60 pKa = 11.84AACFSVLGPLFTDD73 pKa = 5.13DD74 pKa = 3.93PYY76 pKa = 12.0VWLFLEE82 pKa = 4.36NN83 pKa = 3.43

Molecular weight: 9.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G1QQI5|A0A6G1QQI5_9TELE Cytochrome b-c1 complex subunit 2 OS=Channa argus OX=215402 GN=EXN66_Car020288 PE=4 SV=1
MM1 pKa = 7.45WRR3 pKa = 11.84RR4 pKa = 11.84GTLSQKK10 pKa = 10.04VPALAFGRR18 pKa = 11.84RR19 pKa = 11.84NAANIWRR26 pKa = 11.84GPHH29 pKa = 5.57RR30 pKa = 11.84LKK32 pKa = 11.13NKK34 pKa = 9.22GSHH37 pKa = 6.25PGRR40 pKa = 11.84RR41 pKa = 11.84LLPWKK46 pKa = 9.94NKK48 pKa = 9.47KK49 pKa = 9.69FSRR52 pKa = 11.84GKK54 pKa = 10.23NRR56 pKa = 11.84TKK58 pKa = 10.02KK59 pKa = 8.31TT60 pKa = 3.32

Molecular weight: 7.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22260

0

22260

12391188

49

31663

556.7

62.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.357 ± 0.015

2.27 ± 0.014

5.15 ± 0.012

6.885 ± 0.025

3.538 ± 0.014

6.234 ± 0.02

2.686 ± 0.011

4.447 ± 0.015

5.75 ± 0.024

9.487 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.368 ± 0.008

3.94 ± 0.01

5.677 ± 0.022

4.823 ± 0.016

5.486 ± 0.014

8.864 ± 0.022

5.845 ± 0.02

6.345 ± 0.018

1.135 ± 0.007

2.713 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski